Restriction map for
pFN21AE5002
(Circular) (Six-base) MAPSORT of: pFN21AE5002.seq Check: 1751 from: 1 to: 6613
pFN21AE5002 6613 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4423 278
Size: 53 83 186 936 2887 2468
Fragments arranged by size:
2887 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2569 3956 1937
Size: 632 1387 4594
Fragments arranged by size:
4594 1387 632
Acc65I G'GTAC_C
Cuts at: 2931 3934 2931
Size: 1003 5610
AclI AA'CG_TT
Cuts at: 1563 2280 4740 5113 1563
Size: 717 2460 373 3063
Fragments arranged by size:
3063 2460 717 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2647 2885 2895 3494 3863 4676 6060 6328
Size: 1015 238 10 599 369 813 1384 268
Cuts at: 6328 1632
Size: 1917
Fragments arranged by size:
1917 1384 1015 813 599 369 268 238
10
AflII C'TTAA_G
Cuts at: 820 1017 2554 820
Size: 197 1537 4879
Fragments arranged by size:
4879 1537 197
AflIII A'CryG_T
Cuts at: 1203 2988 3247 3854 5512 6489 1203
Size: 1785 259 607 1658 977 1327
Fragments arranged by size:
1785 1658 1327 977 607 259
AgeI A'CCGG_T
Cuts at: 5444 5444
Size: 6613
AhdI GACnn_n'nnGTC
Cuts at: 5342 5342
Size: 6613
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6613
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3623 1184
Size: 2439 4174
AlwNI CAG_nnn'CTG
Cuts at: 3401 3493 3698 4356 5928 3401
Size: 92 205 658 1572 4086
Fragments arranged by size:
4086 1572 658 205 92
ApaLI G'TGCA_C
Cuts at: 4670 5826 4670
Size: 1156 5457
ApoI r'AATT_y
Cuts at: 1493 2578 2631 2816 3230 3299 3354 3722 3922
Size: 1085 53 185 414 69 55 368 200
Cuts at: 3922 4148 4300 6409 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1085 414 368 226 200 185
152 69 55 53
AseI AT'TA_AT
Cuts at: 160 1032 5167 160
Size: 872 4135 1606
Fragments arranged by size:
4135 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6613
AvaI C'yCGr_G
Cuts at: 1870 1943 3938 1870
Size: 73 1995 4545
Fragments arranged by size:
4545 1995 73
BamHI G'GATC_C
Cuts at: 3943 4310 3943
Size: 367 6246
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2044 2931 3934 5389 618
Size: 325 217 627 257 887 1003 1455 1842
Fragments arranged by size:
1842 1455 1003 887 627 325 257 217
BanII G_rGCy'C
Cuts at: 1391 3932 1391
Size: 2541 4072
BbeI G_GCGC'C
Cuts at: 2048 2048
Size: 6613
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2149 2249 3638 928
Size: 971 250 100 1389 3903
Fragments arranged by size:
3903 1389 971 250 100
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4804 4838 1903
Size: 34 50 34 2783 34 3678
Fragments arranged by size:
3678 2783 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2223 4505 5715 2223
Size: 2282 1210 3121
Fragments arranged by size:
3121 2282 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6613
BfrBI ATG'CAT
Cuts at: 2595 2595
Size: 6613
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5224 136
Size: 107 122 71 631 605 3552 1525
Fragments arranged by size:
3552 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6608 6608
Size: 6613
BlpI GC'TnA_GC
Cuts at: 4030 4030
Size: 6613
Bme1580I G_kGCm'C
Cuts at: 1790 4674 5830 1790
Size: 2884 1156 2573
Fragments arranged by size:
2884 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6613
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2257 2849 2916
Size: 416 53 83 399 24 840 592 67
Cuts at: 2916 4344 5302 442
Size: 1428 958 1753
Fragments arranged by size:
1753 1428 958 840 592 416 399 83
67 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6613
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5273 1239
Size: 4034 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4738 5603 5901 4738
Size: 865 298 5450
Fragments arranged by size:
5450 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5276 882
Size: 617 3777 2219
Fragments arranged by size:
3777 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4404 493
Size: 711 3200 2702
Fragments arranged by size:
3200 2702 711
BsaBI GATnn'nnATC
Cuts at: 3200 6607 3200
Size: 3407 3206
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2045 2067 2349 4420
Size: 53 83 186 936 512 22 282 2071
Cuts at: 4420 4802 5437 275
Size: 382 635 1451
Fragments arranged by size:
2071 1451 936 635 512 382 282 186
83 53 22
BsaWI w'CCGG_w
Cuts at: 2014 5046 5444 5718 5865 6276 2014
Size: 3032 398 274 147 411 2351
Fragments arranged by size:
3032 2351 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2054 2084 1178
Size: 30 165 30 651 30 5707
Fragments arranged by size:
5707 651 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2062 2105 3035 1186
Size: 876 43 930 4764
Fragments arranged by size:
4764 930 876 43
BseYI C'CCAG_C
Cuts at: 5816 6312 5816
Size: 496 6117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6040
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4063 4824 5852 1540
Size: 275 186 2062 761 1028 2301
Fragments arranged by size:
2301 2062 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4674 4759 5830 4674
Size: 85 1071 5457
Fragments arranged by size:
5457 1071 85
BsmI GAATG_Cn'
Cuts at: 4124 4217 4124
Size: 93 6520
BsmBI CGTCTCn'nnnn_
Cuts at: 2074 2096 2074
Size: 22 6591
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3932 4674 4759 5830 1391
Size: 399 2142 742 85 1071 2174
Fragments arranged by size:
2174 2142 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6276 6276
Size: 6613
BspHI T'CATG_A
Cuts at: 3012 4502 6232 3012
Size: 1490 1730 3393
Fragments arranged by size:
3393 1730 1490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3968 844
Size: 3124 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4500 1394
Size: 429 2677 3507
Fragments arranged by size:
3507 2677 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5108 5282 65
Size: 1183 3860 174 1396
Fragments arranged by size:
3860 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2041 5257 5444 1700
Size: 110 231 3216 187 2869
Fragments arranged by size:
3216 2869 231 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6613
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6613
BssSI C'ACGA_G
Cuts at: 3050 4673 5685 3050
Size: 1623 1012 3978
Fragments arranged by size:
3978 1623 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6613
BstBI TT'CG_AA
Cuts at: 2160 5417 2160
Size: 3257 3356
BstEII G'GTnAC_C
Cuts at: 1184 2620 1184
Size: 1436 5177
BstYI r'GATC_y
Cuts at: 1972 3195 3943 4310 4695 4712 6164 6250 6262
Size: 1223 748 367 385 17 1452 86 12
Cuts at: 6262 6608 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1223 748 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2141 2141
Size: 6613
BtgI C'CryG_G
Cuts at: 513 1065 3828 513
Size: 552 2763 3298
Fragments arranged by size:
3298 2763 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5874
Fragments arranged by size:
5874 683 56
BtsI GCAGTG_nn'
Cuts at: 3423 3465 4129 4923 4943 3423
Size: 42 664 794 20 5093
Fragments arranged by size:
5093 794 664 42 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6613
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2355 2390 529
Size: 35 1791 35 4752
Fragments arranged by size:
4752 1791 35 35
DraI TTT'AAA
Cuts at: 3917 3917
Size: 6613
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5620 809
Size: 507 4304 1802
Fragments arranged by size:
4304 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2846 4060 4949
Size: 54 1425 50 275 18 1016 1214 889
Cuts at: 4949 6578 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1214 1016 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4060 1537
Size: 275 2248 4090
Fragments arranged by size:
4090 2248 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2157 2163 4543 1353
Size: 804 6 2380 3423
Fragments arranged by size:
3423 2380 804 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5200 5574 5720 387
Size: 1064 138 3611 374 146 1280
Fragments arranged by size:
3611 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2647 2885 2895 3494 3863 4676 5273
Size: 393 1015 238 10 599 369 813 597
Cuts at: 5273 6060 6328 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1015 813 787 599 597 393 369
268 238 10
EcoO109I rG'GnC_Cy
Cuts at: 2145 2905 2145
Size: 760 5853
EcoRI G'AATT_C
Cuts at: 3922 4300 3922
Size: 378 6235
EcoRV GAT'ATC
Cuts at: 3382 3382
Size: 6613
FspI TGC'GCA
Cuts at: 5119 5119
Size: 6613
HaeII r_GCGC'y
Cuts at: 1678 2048 5760 1678
Size: 370 3712 2531
Fragments arranged by size:
3712 2531 370
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3371 3403 5254 5286 5328
Size: 32 135 32 1799 32 1851 32 42
Cuts at: 5328 5360 1373
Size: 32 2626
Fragments arranged by size:
2626 1851 1799 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2802 3957 4203 669
Size: 988 281 864 1155 246 3079
Fragments arranged by size:
3079 1155 988 864 281 246
HindIII A'AGCT_T
Cuts at: 748 3100 748
Size: 2352 4261
HpaI GTT'AAC
Cuts at: 4203 4203
Size: 6613
KasI G'GCGC_C
Cuts at: 2044 2044
Size: 6613
KpnI G_GTAC'C
Cuts at: 2935 3938 2935
Size: 1003 5610
MfeI C'AATT_G
Cuts at: 4212 4212
Size: 6613
MluI A'CGCG_T
Cuts at: 6489 6489
Size: 6613
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2263 5727 5911 1305
Size: 958 3464 184 2007
Fragments arranged by size:
3464 2007 958 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2848 10
Size: 54 1425 343 1016 3775
Fragments arranged by size:
3775 1425 1016 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3605 4571 4930 5089 518
Size: 648 2439 966 359 159 2042
Fragments arranged by size:
2439 2042 966 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3490 4029 4706 5854 6099 1606
Size: 1884 539 677 1148 245 2120
Fragments arranged by size:
2120 1884 1148 677 539 245
NaeI GCC'GGC
Cuts at: 2043 2043
Size: 6613
NarI GG'CG_CC
Cuts at: 2045 2045
Size: 6613
NcoI C'CATG_G
Cuts at: 513 1065 3828 513
Size: 552 2763 3298
Fragments arranged by size:
3298 2763 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6613
NgoMIV G'CCGG_C
Cuts at: 2041 2041
Size: 6613
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6613
NotI GC'GGCC_GC
Cuts at: 4060 4060
Size: 6613
NsiI A_TGCA'T
Cuts at: 2597 2597
Size: 6613
NspI r_CATG'y
Cuts at: 1132 1229 3251 3604 3858 3971 5516 1132
Size: 97 2022 353 254 113 1545 2229
Fragments arranged by size:
2229 2022 1545 353 254 113 97
PasI CC'CwG_GG
Cuts at: 1348 2026 1348
Size: 678 5935
PciI A'CATG_T
Cuts at: 3247 3854 5512 3247
Size: 607 1658 4348
Fragments arranged by size:
4348 1658 607
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6613
PfoI T'CCnGG_A
Cuts at: 2451 2451
Size: 6613
PmeI GTTT'AAAC
Cuts at: 3917 3917
Size: 6613
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4682 4714 6195 6227 4682
Size: 32 1481 32 5068
Fragments arranged by size:
5068 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2905 2905
Size: 6613
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6613
PsiI TTA'TAA
Cuts at: 4183 4183
Size: 6613
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2966 2998 2966
Size: 32 6581
PstI C_TGCA'G
Cuts at: 830 2181 3460 3586 3965 830
Size: 1351 1279 126 379 3478
Fragments arranged by size:
3478 1351 1279 379 126
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6613
PvuII CAG'CTG
Cuts at: 3490 3490
Size: 6613
SalI G'TCGA_C
Cuts at: 1936 3955 1936
Size: 2019 4594
SanDI GG'GwC_CC
Cuts at: 2905 2905
Size: 6613
SbfI CC_TGCA'GG
Cuts at: 3965 3965
Size: 6613
ScaI AGT'ACT
Cuts at: 4861 6573 4861
Size: 1712 4901
SfcI C'TryA_G
Cuts at: 826 1045 2177 2420 2528 3456 3582 3961 5096
Size: 219 1132 243 108 928 126 379 1135
Cuts at: 5096 5777 5968 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1132 928 681 379 243 219
191 126 108
SfoI GGC'GCC
Cuts at: 2046 2046
Size: 6613
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6613
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6613
SmaI CCC'GGG
Cuts at: 1872 3940 1872
Size: 2068 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2554 4717 5618 5880 820
Size: 197 926 611 2163 901 262 1553
Fragments arranged by size:
2163 1553 926 901 611 262 197
SnaBI TAC'GTA
Cuts at: 493 4404 493
Size: 3911 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6613
SphI G_CATG'C
Cuts at: 1132 3971 1132
Size: 2839 3774
SspI AAT'ATT
Cuts at: 5 52 4537 5
Size: 47 4485 2081
Fragments arranged by size:
4485 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6613
StyI C'CwwG_G
Cuts at: 513 1065 2097 3678 3828 4052 513
Size: 552 1032 1581 150 224 3074
Fragments arranged by size:
3074 1581 1032 552 224 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2135 3163 3289 3304 4640 4657 4810
Size: 1255 555 1028 126 15 1336 17 153
Cuts at: 4810 4995 5440 325
Size: 185 445 1498
Fragments arranged by size:
1498 1336 1255 1028 555 445 185 153
126 17 15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2323 3114 3130
Size: 274 80 33 51 1443 346 791 16
Cuts at: 3130 4859 6571 96
Size: 1729 1712 138
Fragments arranged by size:
1729 1712 1443 791 346 274 138 80
51 33 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6573 6605 6573
Size: 32 6581
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6613
XbaI T'CTAG_A
Cuts at: 3949 3949
Size: 6613
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6613
XmaI C'CCGG_G
Cuts at: 1870 3938 1870
Size: 2068 4545
XmnI GAAnn'nnTTC
Cuts at: 2819 3300 3780 4742 2819
Size: 481 480 962 4690
Fragments arranged by size:
4690 962 481 480
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4421 276
Size: 53 83 186 936 2887 2468
Fragments arranged by size:
2887 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI
FalI FseI FspAI NruI PacI PmlI PspOMI PspXI
RsrII SacI SacII SapI SexAI SfiI SrfI SwaI
XcmI