MID2 ORF clone pFN21AE4213

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE4213

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Clone info.

Species

human

Clone Name

pFN21AE4213 Sequence (with vector) Restriction Map

Product ID

FHC12697

ORF Sequence

DNA sequence (2115 bp)
Protein sequence (705 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4213

Symbol

MID2

Alias : FXY2, MRX101, RNF60, TRIM1, XLID101

Description

midline 2, transcript variant 2

Original Clone ID

cp94213

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2115 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 705 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS14533.2 3.9e-206 100.0 MID2 CCDS14138.1 6.9e-155 76.2 MID1 CCDS14532.2 6.2e-128 95.9 MID2 CCDS75952.1 1.4e-59 70.5 MID1 CCDS75953.1 4.1e-43 65.0 MID1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR003879 502 519 PR01407 Butyrophylin-like IPR003879 543 567 PR01407 Butyrophylin-like IPR003879 615 639 PR01407 Butyrophylin-like HMMPfam IPR018957 30 79 PF00097 Zinc finger IPR000315 193 231 PF00643 Zinc finger IPR003961 414 491 PF00041 Fibronectin IPR003877 558 667 PF00622 SPla/RYanodine receptor SPRY HMMSmart IPR001841 30 79 SM00184 Zinc finger IPR000315 134 184 SM00336 Zinc finger IPR000315 190 232 SM00336 Zinc finger IPR003649 239 365 SM00502 B-box IPR003961 402 492 SM00060 Fibronectin IPR018355 558 676 SM00449 SPla/RYanodine receptor subgroup ProfileScan IPR001841 30 80 PS50089 Zinc finger IPR000315 190 232 PS50119 Zinc finger IPR017903 340 399 PS51262 COS domain IPR003961 398 501 PS50853 Fibronectin IPR001870 486 679 PS50188 B302/SPRY domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_438112 705 300204,300928 100.0 100.0 XP_016884728 685 300204,300928 100.0 97.2 NP_000372 667 189960,300000,300552 76.2 94.3 NP_001180206 667 189960,300000,300552 76.2 94.3 XP_016885025 667 189960,300000,300552 76.2 94.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4213 Download

>KIEE4213  2115 bp
ATGGGTGAAAGCCCAGCCTCCGTGGTTCTTAATGCCTCAGGAGGACTATTTTCACTAAAG
ATGGAAACACTGGAGTCTGAATTGACCTGTCCAATCTGCCTAGAGTTGTTTGAAGACCCC
CTTCTGCTCCCTTGTGCTCACAGCCTCTGCTTCAGCTGTGCCCATCGCATTTTGGTATCA
AGCTGCAGCTCTGGTGAATCCATTGAACCCATTACTGCTTTCCAGTGTCCTACCTGCAGG
TATGTTATCTCGCTGAACCACCGGGGCCTGGATGGCCTCAAGAGGAATGTGACTCTGCAG
AACATTATTGATCGCTTCCAGAAGGCTTCAGTCAGTGGGCCCAATTCCCCTAGTGAGAGC
CGCCGGGAAAGGACTTACAGGCCCACCACTGCCATGTCTAGCGAGCGAATTGCTTGCCAA
TTCTGTGAGCAGGACCCGCCAAGGGATGCAGTAAAAACATGCATCACCTGTGAGGTCTCC
TACTGTGACCGTTGCCTGCGGGCCACGCACCCCAACAAGAAACCTTTCACCAGCCACCGC
CTGGTGGAACCAGTGCCAGACACACATCTTCGAGGGATCACCTGCCTGGACCATGAGAAT
GAGAAAGTGAACATGTACTGTGTATCTGATGACCAATTGATCTGTGCCTTATGCAAACTG
GTGGGTCGTCACCGAGACCATCAGGTCGCATCCCTGAATGATCGATTTGAGAAACTCAAG
CAAACTCTGGAGATGAACCTCACCAACCTGGTTAAGCGCAACAGCGAACTAGAAAATCAA
ATGGCCAAACTAATACAGATCTGCCAGCAGGTTGAGGTGAATACTGCTATGCATGAGGCA
AAACTTATGGAAGAATGTGACGAGTTGGTAGAGATCATCCAGCAGAGGAAGCAAATGATC
GCTGTCAAAATCAAAGAGACAAAGGTTATGAAACTGAGAAAGTTGGCACAGCAGGTTGCT
AATTGCCGCCAGTGTCTTGAACGGTCAACAGTCCTCATCAACCAAGCTGAGCATATCCTG
AAAGAAAATGACCAGGCACGGTTTCTACAGTCTGCAAAAAATATTGCTGAGAGGGTCGCT
ATGGCAACTGCATCTTCTCAAGTTCTGATTCCAGACATCAATTTTAATGATGCCTTTGAA
AACTTTGCTTTAGATTTTTCCAGAGAAAAGAAACTGCTAGAGGGGTTAGATTATTTAACA
GCCCCAAACCCACCATCTATCCGAGAAGAACTCTGTACTGCCTCCCATGACACCATTACA
GTCCACTGGATCTCGGATGATGAGTTCAGCATCAGCTCCTATGAGCTTCAGTACACCATA
TTCACTGGCCAGGCTAACTTCATCAGCCTGTATAATTCAGTAGACAGCTGGATGATTGTT
CCCAACATTAAACAGAACCATTACACAGTGCATGGACTCCAGAGCGGGACTCGCTACATC
TTCATCGTTAAAGCCATAAACCAAGCCGGCAGCCGGAACAGTGAACCTACCCGACTAAAA
ACAAACAGCCAACCCTTTAAATTGGATCCCAAAATGACTCACAAGAAGTTGAAGATCTCC
AATGATGGATTGCAGATGGAGAAGGATGAAAGCTCTCTAAAGAAGAGCCACACCCCAGAG
AGGTTTAGTGGCACAGGGTGCTATGGGGCAGCAGGAAATATATTCATTGACAGTGGCTGC
CACTATTGGGAGGTGGTCATGGGTTCCTCAACATGGTATGCAATTGGCATTGCCTACAAA
TCAGCTCCAAAGAATGAATGGATTGGCAAGAATGCCTCCTCATGGGTCTTCTCTCGCTGC
AATAGTAACTTCGTGGTGAGACACAACAACAAGGAAATGCTGGTGGATGTGCCCCCACAC
CTGAAGCGTCTGGGTGTCCTCCTGGATTATGACAACAATATGCTGTCTTTCTATGACCCA
GCTAACTCTCTCCATCTTCATACTTTTGATGTGACCTTCATTCTTCCAGTTTGTCCAACA
TTTACAATCTGGAACAAATCCCTAATGATCCTGTCTGGCTTGCCTGCCCCAGATTTTATT
GATTACCCTGAGCGGCAGGAATGCAACTGCAGGCCTCAAGAATCCCCTTATGTTTCTGGG
ATGAAAACCTGTCAT

ORF Protein

Cloned ORF protein sequence for pFN21AE4213 Download

>KIEE4213  705 aa
MGESPASVVLNASGGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVS
SCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSES
RRERTYRPTTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHR
LVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLK
QTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMI
AVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVA
MATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTIT
VHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYI
FIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPE
RFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRC
NSNFVVRHNNKEMLVDVPPHLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPT
FTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4213 Download

>pFN21AE4213  6820 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGTGAAAGCCCAGCCTCCGTGGTTCTTAATGC
CTCAGGAGGACTATTTTCACTAAAGATGGAAACACTGGAGTCTGAATTGACCTGTCCAAT
CTGCCTAGAGTTGTTTGAAGACCCCCTTCTGCTCCCTTGTGCTCACAGCCTCTGCTTCAG
CTGTGCCCATCGCATTTTGGTATCAAGCTGCAGCTCTGGTGAATCCATTGAACCCATTAC
TGCTTTCCAGTGTCCTACCTGCAGGTATGTTATCTCGCTGAACCACCGGGGCCTGGATGG
CCTCAAGAGGAATGTGACTCTGCAGAACATTATTGATCGCTTCCAGAAGGCTTCAGTCAG
TGGGCCCAATTCCCCTAGTGAGAGCCGCCGGGAAAGGACTTACAGGCCCACCACTGCCAT
GTCTAGCGAGCGAATTGCTTGCCAATTCTGTGAGCAGGACCCGCCAAGGGATGCAGTAAA
AACATGCATCACCTGTGAGGTCTCCTACTGTGACCGTTGCCTGCGGGCCACGCACCCCAA
CAAGAAACCTTTCACCAGCCACCGCCTGGTGGAACCAGTGCCAGACACACATCTTCGAGG
GATCACCTGCCTGGACCATGAGAATGAGAAAGTGAACATGTACTGTGTATCTGATGACCA
ATTGATCTGTGCCTTATGCAAACTGGTGGGTCGTCACCGAGACCATCAGGTCGCATCCCT
GAATGATCGATTTGAGAAACTCAAGCAAACTCTGGAGATGAACCTCACCAACCTGGTTAA
GCGCAACAGCGAACTAGAAAATCAAATGGCCAAACTAATACAGATCTGCCAGCAGGTTGA
GGTGAATACTGCTATGCATGAGGCAAAACTTATGGAAGAATGTGACGAGTTGGTAGAGAT
CATCCAGCAGAGGAAGCAAATGATCGCTGTCAAAATCAAAGAGACAAAGGTTATGAAACT
GAGAAAGTTGGCACAGCAGGTTGCTAATTGCCGCCAGTGTCTTGAACGGTCAACAGTCCT
CATCAACCAAGCTGAGCATATCCTGAAAGAAAATGACCAGGCACGGTTTCTACAGTCTGC
AAAAAATATTGCTGAGAGGGTCGCTATGGCAACTGCATCTTCTCAAGTTCTGATTCCAGA
CATCAATTTTAATGATGCCTTTGAAAACTTTGCTTTAGATTTTTCCAGAGAAAAGAAACT
GCTAGAGGGGTTAGATTATTTAACAGCCCCAAACCCACCATCTATCCGAGAAGAACTCTG
TACTGCCTCCCATGACACCATTACAGTCCACTGGATCTCGGATGATGAGTTCAGCATCAG
CTCCTATGAGCTTCAGTACACCATATTCACTGGCCAGGCTAACTTCATCAGCCTGTATAA
TTCAGTAGACAGCTGGATGATTGTTCCCAACATTAAACAGAACCATTACACAGTGCATGG
ACTCCAGAGCGGGACTCGCTACATCTTCATCGTTAAAGCCATAAACCAAGCCGGCAGCCG
GAACAGTGAACCTACCCGACTAAAAACAAACAGCCAACCCTTTAAATTGGATCCCAAAAT
GACTCACAAGAAGTTGAAGATCTCCAATGATGGATTGCAGATGGAGAAGGATGAAAGCTC
TCTAAAGAAGAGCCACACCCCAGAGAGGTTTAGTGGCACAGGGTGCTATGGGGCAGCAGG
AAATATATTCATTGACAGTGGCTGCCACTATTGGGAGGTGGTCATGGGTTCCTCAACATG
GTATGCAATTGGCATTGCCTACAAATCAGCTCCAAAGAATGAATGGATTGGCAAGAATGC
CTCCTCATGGGTCTTCTCTCGCTGCAATAGTAACTTCGTGGTGAGACACAACAACAAGGA
AATGCTGGTGGATGTGCCCCCACACCTGAAGCGTCTGGGTGTCCTCCTGGATTATGACAA
CAATATGCTGTCTTTCTATGACCCAGCTAACTCTCTCCATCTTCATACTTTTGATGTGAC
CTTCATTCTTCCAGTTTGTCCAACATTTACAATCTGGAACAAATCCCTAATGATCCTGTC
TGGCTTGCCTGCCCCAGATTTTATTGATTACCCTGAGCGGCAGGAATGCAACTGCAGGCC
TCAAGAATCCCCTTATGTTTCTGGGATGAAAACCTGTCATGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT