(Linear) (Six-base) MAPSORT of: KIEE4213.seq Check: 5962 from: 1 to: 2115 KIEE4213.orf With 182 enzymes: SgfI * July 3, 2012 18:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 589 2115 Size: 589 1526 AccI GT'mk_AC Cuts at: 0 1361 2115 Size: 1361 754 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 135 311 1291 1882 2115 Size: 135 176 980 591 233 Fragments arranged by size: 980 591 233 176 135 AflIII A'CryG_T Cuts at: 0 611 2115 Size: 611 1504 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1267 1299 2115 Size: 1267 32 816 Fragments arranged by size: 1267 816 32 AlwNI CAG_nnn'CTG Cuts at: 0 146 1676 2115 Size: 146 1530 439 Fragments arranged by size: 1530 439 146 ApaI G_GGCC'C Cuts at: 0 341 2115 Size: 341 1774 BamHI G'GATC_C Cuts at: 0 1524 2115 Size: 1524 591 BanII G_rGCy'C Cuts at: 0 341 2115 Size: 341 1774 BbsI GAAGACnn'nnnn_ Cuts at: 0 119 1779 2115 Size: 119 1660 336 Fragments arranged by size: 1660 336 119 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 391 395 425 429 2115 Size: 391 4 30 4 1686 Fragments arranged by size: 1686 391 30 4 4 BfrBI ATG'CAT Cuts at: 0 461 831 2115 Size: 461 370 1284 Fragments arranged by size: 1284 461 370 BglI GCCn_nnn'nGGC Cuts at: 0 272 2115 Size: 272 1843 BglII A'GATC_T Cuts at: 0 797 1553 2115 Size: 797 756 562 Fragments arranged by size: 797 756 562 BlpI GC'TnA_GC Cuts at: 0 1007 2115 Size: 1007 1108 Bme1580I G_kGCm'C Cuts at: 0 162 341 1853 2115 Size: 162 179 1512 262 Fragments arranged by size: 1512 262 179 162 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1246 1445 2115 Size: 1246 199 670 Fragments arranged by size: 1246 670 199 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 91 748 1402 2115 Size: 91 657 654 713 Fragments arranged by size: 713 657 654 91 Bpu10I CC'TnA_GC Cuts at: 0 2048 2115 Size: 2048 67 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 262 700 1082 2060 2115 Size: 262 438 382 978 55 Fragments arranged by size: 978 438 382 262 55 BsaI GGTCTCn'nnnn_ Cuts at: 0 480 668 2115 Size: 480 188 1447 Fragments arranged by size: 1447 480 188 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1767 2115 Size: 1767 348 BseYI C'CCAG_C Cuts at: 0 12 1917 2115 Size: 12 1905 198 Fragments arranged by size: 1905 198 12 BsiHKAI G_wGCw'C Cuts at: 0 138 2115 Size: 138 1977 BsmI GAATG_Cn' Cuts at: 0 1776 2065 2115 Size: 1776 289 50 Fragments arranged by size: 1776 289 50 Bsp1286I G_dGCh'C Cuts at: 0 138 162 341 1853 2115 Size: 138 24 179 1512 262 Fragments arranged by size: 1512 262 179 138 24 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 227 241 589 798 942 2115 Size: 227 14 348 209 144 1173 Fragments arranged by size: 1173 348 227 209 144 14 BsrBI CCG'CTC Cuts at: 0 1424 2052 2115 Size: 1424 628 63 Fragments arranged by size: 1424 628 63 BsrDI GCAATG_nn' Cuts at: 0 1727 2115 Size: 1727 388 BsrFI r'CCGG_y Cuts at: 0 1465 2115 Size: 1465 650 BstAPI GCAn_nnn'nTGC Cuts at: 0 1637 2115 Size: 1637 478 BstYI r'GATC_y Cuts at: 0 797 1268 1524 1553 2115 Size: 797 471 256 29 562 Fragments arranged by size: 797 562 471 256 29 Bsu36I CC'TnA_GG Cuts at: 0 36 2115 Size: 36 2079 BtgI C'CryG_G Cuts at: 0 20 2115 Size: 20 2095 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 148 2115 Size: 148 1967 BtsI GCAGTG_nn' Cuts at: 0 386 2115 Size: 386 1729 ClaI AT'CG_AT Cuts at: 0 702 2115 Size: 702 1413 DraI TTT'AAA Cuts at: 0 1518 2115 Size: 1518 597 DraIII CAC_nnn'GTG Cuts at: 0 1637 2115 Size: 1637 478 DrdI GACnn_nn'nnGTC Cuts at: 0 682 2115 Size: 682 1433 EaeI y'GGCC_r Cuts at: 0 782 1326 2115 Size: 782 544 789 Fragments arranged by size: 789 782 544 EarI CTCTTCn'nnn_ Cuts at: 0 1597 2115 Size: 1597 518 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 91 135 311 748 1291 1402 1882 2115 Size: 91 44 176 437 543 111 480 233 Fragments arranged by size: 543 480 437 233 176 111 91 44 EcoO109I rG'GnC_Cy Cuts at: 0 264 432 2115 Size: 264 168 1683 Fragments arranged by size: 1683 264 168 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 136 2115 Size: 136 1979 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1576 1608 2115 Size: 1576 32 507 Fragments arranged by size: 1576 507 32 HincII GTy'rAC Cuts at: 0 986 2115 Size: 986 1129 MfeI C'AATT_G Cuts at: 0 634 1721 2115 Size: 634 1087 394 Fragments arranged by size: 1087 634 394 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1999 2115 Size: 1999 116 MscI TGG'CCA Cuts at: 0 784 1328 2115 Size: 784 544 787 Fragments arranged by size: 787 784 544 MslI CAynn'nnrTG Cuts at: 0 391 596 2115 Size: 391 205 1519 Fragments arranged by size: 1519 391 205 MspA1I CmG'CkG Cuts at: 0 155 1367 2115 Size: 155 1212 748 Fragments arranged by size: 1212 748 155 NaeI GCC'GGC Cuts at: 0 1467 2115 Size: 1467 648 NgoMIV G'CCGG_C Cuts at: 0 1465 2115 Size: 1465 650 NsiI A_TGCA'T Cuts at: 0 463 833 2115 Size: 463 370 1282 Fragments arranged by size: 1282 463 370 NspI r_CATG'y Cuts at: 0 461 615 2115 Size: 461 154 1500 Fragments arranged by size: 1500 461 154 PciI A'CATG_T Cuts at: 0 611 2115 Size: 611 1504 PflMI CCAn_nnn'nTGG Cuts at: 0 540 2115 Size: 540 1575 PfoI T'CCnGG_A Cuts at: 0 1880 2115 Size: 1880 235 PpuMI rG'GwC_Cy Cuts at: 0 432 2115 Size: 432 1683 PspOMI G'GGCC_C Cuts at: 0 337 2115 Size: 337 1778 PstI C_TGCA'G Cuts at: 0 187 238 299 2071 2115 Size: 187 51 61 1772 44 Fragments arranged by size: 1772 187 61 51 44 PvuII CAG'CTG Cuts at: 0 155 1367 2115 Size: 155 1212 748 Fragments arranged by size: 1212 748 155 SapI GCTCTTCn'nnn_ Cuts at: 0 1597 2115 Size: 1597 518 SbfI CC_TGCA'GG Cuts at: 0 238 2115 Size: 238 1877 SexAI A'CCwGG_T Cuts at: 0 746 2115 Size: 746 1369 SfcI C'TryA_G Cuts at: 0 183 234 295 1045 2067 2115 Size: 183 51 61 750 1022 48 Fragments arranged by size: 1022 750 183 61 51 48 SfiI GGCCn_nnn'nGGCC Cuts at: 0 272 2115 Size: 272 1843 SmlI C'TyrA_G Cuts at: 0 277 715 1097 2075 2115 Size: 277 438 382 978 40 Fragments arranged by size: 978 438 382 277 40 SspI AAT'ATT Cuts at: 0 1062 2115 Size: 1062 1053 StuI AGG'CCT Cuts at: 0 2073 2115 Size: 2073 42 StyI C'CwwG_G Cuts at: 0 439 2115 Size: 439 1676 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1861 2115 Size: 1861 254 TatI w'GTAC_w Cuts at: 0 614 1234 1310 2115 Size: 614 620 76 805 Fragments arranged by size: 805 620 614 76 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1202 1234 2115 Size: 1202 32 881 Fragments arranged by size: 1202 881 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 537 1754 2115 Size: 537 1217 361 Fragments arranged by size: 1217 537 361 XmnI GAAnn'nnTTC Cuts at: 0 523 2115 Size: 523 1592 Enzymes that do cut: AarI AccI AcuI AflIII AloI AlwNI ApaI BamHI BanII BbsI BcgI BfrBI BglI BglII BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI EarI Eco57MI EcoO109I FalI Hin4I HincII MfeI MmeI MscI MslI MspA1I NaeI NgoMIV NsiI NspI PciI PflMI PfoI PpuMI PspOMI PstI PvuII SapI SbfI SexAI SfcI SfiI SmlI SspI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BciVI BclI BmgBI BmrI BmtI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I CspCI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MluI NarI NcoI NdeI NheI NotI NruI PacI PasI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI ScaI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XhoI XmaI ZraI