Restriction map for
pFN21AE4213
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BanI BbeI
BbrPI BbvCI BciVI BclI BfrI
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspEI BspHI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV FbaI
FseI FspI HaeII Hin1I HindIII
HpaI KasI KpnI KspI MaeII
MamI MluI MroI MvnI NarI
NcoI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI ScaI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 1360 GTAGAC GT^[AC][GT]AC
AflIII 611 ACATGT A^C[GA][CT]GT
AocI 35 CCTCAGG CC^T[ACGT]AGG
ApaI 337 GGGCCC GGGCC^C
Asp700I 519 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 134 GTGCTC G[AT]GC[AT]^C
BamHI 1524 GGATCC G^GATCC
BanII 337 GGGCCC G[GA]GC[CT]^C
BglI 266 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1006 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1006 GCTGAGC GC^T[ACGT]AGC
BseRI 1776 CTCCTC GAGGAG(10/8)
BsiHKAI 134 GTGCTC G[AT]GC[AT]^C
BsmFI 1426 GGGAC GGGAC(10/14)
BspDI 701 ATCGAT AT^CGAT
BspLU11I 611 ACATGT A^CATGT
BsrDI 1728 CATTGC GCAATG(2/0)
BsrFI 1465 GCCGGC [GA]^CCGG[CT]
BstAPI 1631 GCACAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 35 CCTCAGG CC^T[ACGT]AGG
CelII 1006 GCTGAGC GC^T[ACGT]AGC
CfoI 756 GCGC GCG^C
Cfr10I 1465 GCCGGC [GA]^CCGG[CT]
ClaI 701 ATCGAT AT^CGAT
DraI 1516 TTTAAA TTT^AAA
DraIII 1632 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 676 GACCATCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 20 CCGTGG C^C[GA][CT]GG
EarI 1602 GAAGAG CTCTTC(1/4)
Eco81I 35 CCTCAGG CC^T[ACGT]AGG
EcoT14I 439 CCAAGG C^C[AT][AT]GG
HgaI 1866 GCGTC GACGC(5/10)
HhaI 756 GCGC GCG^C
HinP1I 756 GCGC G^CGC
HincII 984 GTCAAC GT[CT]^[GA]AC
HindII 984 GTCAAC GT[CT]^[GA]AC
Ksp632I 1602 GAAGAG CTCTTC(1/4)
NaeI 1465 GCCGGC GCC^GGC
NgoMIV 1465 GCCGGC G^CCGGC
PflMI 534 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 431 AGGACCC [GA]G^G[AT]CC[CT]
SapI 1602 GAAGAGC GCTCTTC(1/4)
SbfI 233 CCTGCAGG CCTGCA^GG
SexAI 746 ACCTGGT A^CC[AT]GGT
SfiI 265 GGCCTGGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 233 CCTGCAGG CCTGCA^GG
SspI 1060 AATATT AAT^ATT
StuI 2071 AGGCCT AGG^CCT
StyI 439 CCAAGG C^C[AT][AT]GG
Van91I 534 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 519 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwNI 141 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1671 CAGTGGCTG
AvaII 432 GGACC G^G[AT]CC
588 GGACC
BalI 782 TGGCCA TGG^CCA
1326 TGGCCA
BbsI 112 GAAGAC GAAGAC(2/6)
1786 GTCTTC
BcgI 402 CGAGCGAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
406 CGAATTGCTTGC
BcnI 261 CCGGG CC^[GC]GG
363 CCGGG
BglII 797 AGATCT A^GATCT
1553 AGATCT
BpuAI 112 GAAGAC GAAGAC(2/6)
1786 GTCTTC
BsaI 674 GAGACC GGTCTC(1/5)
474 GGTCTC
BsmI 1770 GAATGC GAATGC(1/-1)
2059 GAATGC
BsrBI 1422 GAGCGG CCGCTC(-3/-3)
2050 GAGCGG
DraII 263 GGGGCCT [GA]G^G[ACGT]CC[CT]
431 AGGACCC
EaeI 782 TGGCCA [CT]^GGCC[GA]
1326 TGGCCA
EcoO109I 263 GGGGCCT [GA]G^G[ACGT]CC[CT]
431 AGGACCC
EcoT22I 459 ATGCAT ATGCA^T
829 ATGCAT
MfeI 634 CAATTG C^AATTG
1721 CAATTG
MluNI 782 TGGCCA TGG^CCA
1326 TGGCCA
MscI 782 TGGCCA TGG^CCA
1326 TGGCCA
MslI 387 CACTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
592 CATGAGAATG
MspA1I 153 CAGCTG C[AC]G^C[GT]G
1365 CAGCTG
MunI 634 CAATTG C^AATTG
1721 CAATTG
NciI 261 CCGGG CC^[GC]GG
363 CCGGG
NsiI 459 ATGCAT ATGCA^T
829 ATGCAT
NspI 457 ACATGC [GA]CATG^[CT]
611 ACATGT
PvuII 153 CAGCTG CAG^CTG
1365 CAGCTG
TaqI 570 TCGA T^CGA
702 TCGA
TthHB8I 570 TCGA T^CGA
702 TCGA
XcmI 530 CCAGCCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1747 CCAAAGAATGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BfaI BmyI BpmI BsaJI BsiYI
BslI BsmAI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII