Restriction map for
pFN21AE4213
(Circular) (Six-base) MAPSORT of: pFN21AE4213.seq Check: 6116 from: 1 to: 6820
pFN21AE4213 6820 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2594 2594
Size: 6820
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4630 278
Size: 53 83 186 936 3094 2468
Fragments arranged by size:
3094 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3366 4163 1937
Size: 1429 797 4594
Fragments arranged by size:
4594 1429 797
Acc65I G'GTAC_C
Cuts at: 4141 4141
Size: 6820
AclI AA'CG_TT
Cuts at: 1563 4947 5320 1563
Size: 3384 373 3063
Fragments arranged by size:
3384 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2140 2316 3296 3887 4883 6267 6535 1632
Size: 508 176 980 591 996 1384 268 1917
Fragments arranged by size:
1917 1384 996 980 591 508 268 176
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6623
AflIII A'CryG_T
Cuts at: 1203 2616 5719 6696 1203
Size: 1413 3103 977 1327
Fragments arranged by size:
3103 1413 1327 977
AgeI A'CCGG_T
Cuts at: 5651 5651
Size: 6820
AhdI GACnn_n'nnGTC
Cuts at: 5549 5549
Size: 6820
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6820
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6820
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3272 3304 3272
Size: 32 6788
AlwNI CAG_nnn'CTG
Cuts at: 2151 3681 4563 6135 2151
Size: 1530 882 1572 2836
Fragments arranged by size:
2836 1572 1530 882
ApaI G_GGCC'C
Cuts at: 2346 2346
Size: 6820
ApaLI G'TGCA_C
Cuts at: 4877 6033 4877
Size: 1156 5664
ApoI r'AATT_y
Cuts at: 1493 4129 4355 4507 6616 1493
Size: 2636 226 152 2109 1697
Fragments arranged by size:
2636 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5374 160
Size: 872 4342 1606
Fragments arranged by size:
4342 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6820
AvaI C'yCGr_G
Cuts at: 1870 1943 4145 1870
Size: 73 2202 4545
Fragments arranged by size:
4545 2202 73
BamHI G'GATC_C
Cuts at: 3529 4150 4517 3529
Size: 621 367 5832
Fragments arranged by size:
5832 621 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4141 5596 618
Size: 325 217 627 2354 1455 1842
Fragments arranged by size:
2354 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2346 4139 1391
Size: 955 1793 4072
Fragments arranged by size:
4072 1793 955
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2124 3784 928
Size: 971 225 1660 3964
Fragments arranged by size:
3964 1660 971 225
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2396 2400 2430 2434 5011 5045 1903
Size: 34 459 4 30 4 2577 34 3678
Fragments arranged by size:
3678 2577 459 34 34 30 4 4
BciVI GTATCCnnnnn_n'
Cuts at: 4712 5922 4712
Size: 1210 5610
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6820
BfrBI ATG'CAT
Cuts at: 2466 2836 2466
Size: 370 6450
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2277 5431 136
Size: 107 122 71 631 605 605 3154 1525
Fragments arranged by size:
3154 1525 631 605 605 122 107 71
BglII A'GATC_T
Cuts at: 2802 3558 6815 2802
Size: 756 3257 2807
Fragments arranged by size:
3257 2807 756
BlpI GC'TnA_GC
Cuts at: 3012 4237 3012
Size: 1225 5595
Bme1580I G_kGCm'C
Cuts at: 1790 2167 2346 3858 4881 6037 1790
Size: 377 179 1512 1023 1156 2573
Fragments arranged by size:
2573 1512 1156 1023 377 179
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6820
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4551 5509 442
Size: 416 53 83 399 24 3134 958 1753
Fragments arranged by size:
3134 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6820
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3251 3450 3251
Size: 199 6621
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 2753 3407 5480 1239
Size: 857 657 654 2073 2579
Fragments arranged by size:
2579 2073 857 657 654
Bpu10I CC'TnA_GC
Cuts at: 4053 4053
Size: 6820
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2267 2705 3087 4065 4945 5810 6108 2267
Size: 438 382 978 880 865 298 2979
Fragments arranged by size:
2979 978 880 865 438 382 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2485 2673 5483 882
Size: 617 986 188 2810 2219
Fragments arranged by size:
2810 2219 986 617 188
BsaAI yAC'GTr
Cuts at: 493 1204 4611 493
Size: 711 3407 2702
Fragments arranged by size:
3407 2702 711
BsaBI GATnn'nnATC
Cuts at: 6814 6814
Size: 6820
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4627 5009 5644 275
Size: 53 83 186 936 3094 382 635 1451
Fragments arranged by size:
3094 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5253 5651 5925 6072 6483 5253
Size: 398 274 147 411 5590
Fragments arranged by size:
5590 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6595
Fragments arranged by size:
6595 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3772 1186
Size: 2586 4234
BseYI C'CCAG_C
Cuts at: 2017 3922 6023 6519 2017
Size: 1905 2101 496 2318
Fragments arranged by size:
2318 2101 1905 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6247
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4270 5031 6059 1540
Size: 275 186 2269 761 1028 2301
Fragments arranged by size:
2301 2269 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2143 4881 4966 6037 2143
Size: 2738 85 1071 2926
Fragments arranged by size:
2926 2738 1071 85
BsmI GAATG_Cn'
Cuts at: 3781 4070 4331 4424 3781
Size: 289 261 93 6177
Fragments arranged by size:
6177 289 261 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2143 2167 2346 3858 4139 4881 4966
Size: 399 353 24 179 1512 281 742 85
Cuts at: 4966 6037 1391
Size: 1071 2174
Fragments arranged by size:
2174 1512 1071 742 399 353 281 179
85 24
BspEI T'CCGG_A
Cuts at: 6483 6483
Size: 6820
BspHI T'CATG_A
Cuts at: 4709 6439 4709
Size: 1730 5090
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2232 2246 2594 2803 2947 4175 844
Size: 1388 14 348 209 144 1228 3489
Fragments arranged by size:
3489 1388 1228 348 209 144 14
BsrBI CCG'CTC
Cuts at: 1394 1823 3429 4057 4707 1394
Size: 429 1606 628 650 3507
Fragments arranged by size:
3507 1606 650 628 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3732 5315 5489 65
Size: 1183 2484 1583 174 1396
Fragments arranged by size:
2484 1583 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3470 5464 5651 1700
Size: 110 1660 1994 187 2869
Fragments arranged by size:
2869 1994 1660 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6820
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6820
BssSI C'ACGA_G
Cuts at: 4880 5892 4880
Size: 1012 5808
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3642 794
Size: 2848 3972
BstBI TT'CG_AA
Cuts at: 5624 5624
Size: 6820
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6820
BstYI r'GATC_y
Cuts at: 1972 2802 3273 3529 3558 4150 4517 4902 4919
Size: 830 471 256 29 592 367 385 17
Cuts at: 4919 6371 6457 6469 6815 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 830 592 471 385 367 346
256 86 29 17 12
Bsu36I CC'TnA_GG
Cuts at: 2041 2041
Size: 6820
BtgI C'CryG_G
Cuts at: 513 1065 2004 2025 513
Size: 552 939 21 5308
Fragments arranged by size:
5308 939 552 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2153 487
Size: 683 56 927 5154
Fragments arranged by size:
5154 927 683 56
BtsI GCAGTG_nn'
Cuts at: 2391 4336 5130 5150 2391
Size: 1945 794 20 4061
Fragments arranged by size:
4061 1945 794 20
ClaI AT'CG_AT
Cuts at: 1554 2707 1554
Size: 1153 5667
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6785
DraI TTT'AAA
Cuts at: 3523 4124 3523
Size: 601 6219
DraIII CAC_nnn'GTG
Cuts at: 3642 3642
Size: 6820
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2687 5827 809
Size: 507 1371 3140 1802
Fragments arranged by size:
3140 1802 1371 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2787 3331 4267
Size: 54 1425 50 275 18 957 544 936
Cuts at: 4267 5156 6785 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 957 936 889 544 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4267 1537
Size: 275 2455 4090
Fragments arranged by size:
4090 2455 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3602 4750 1353
Size: 2249 1148 3423
Fragments arranged by size:
3423 2249 1148
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5407 5781 5927 387
Size: 1064 138 3818 374 146 1280
Fragments arranged by size:
3818 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2140 2316 2753 3296 3407 3887
Size: 393 464 44 176 437 543 111 480
Cuts at: 3887 4883 5480 6267 6535 1239
Size: 996 597 787 268 1524
Fragments arranged by size:
1524 996 787 597 543 480 464 437
393 268 176 111 44
EcoO109I rG'GnC_Cy
Cuts at: 2269 2437 2269
Size: 168 6652
EcoRI G'AATT_C
Cuts at: 4129 4507 4129
Size: 378 6442
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2141 2141
Size: 6820
FspI TGC'GCA
Cuts at: 5326 5326
Size: 6820
HaeII r_GCGC'y
Cuts at: 1678 5967 1678
Size: 4289 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3581 3613 5461 5493 5535
Size: 32 135 32 2009 32 1848 32 42
Cuts at: 5535 5567 1373
Size: 32 2626
Fragments arranged by size:
2626 2009 1848 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2991 4164 4410 669
Size: 988 281 1053 1173 246 3079
Fragments arranged by size:
3079 1173 1053 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6820
HpaI GTT'AAC
Cuts at: 4410 4410
Size: 6820
KpnI G_GTAC'C
Cuts at: 4145 4145
Size: 6820
MfeI C'AATT_G
Cuts at: 2639 3726 4419 2639
Size: 1087 693 5040
Fragments arranged by size:
5040 1087 693
MluI A'CGCG_T
Cuts at: 6696 6696
Size: 6820
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4004 5934 6118 1305
Size: 2699 1930 184 2007
Fragments arranged by size:
2699 2007 1930 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2789 3333 10
Size: 54 1425 343 957 544 3497
Fragments arranged by size:
3497 1425 957 544 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2396 2601 4778 5137 5296 518
Size: 648 1230 205 2177 359 159 2042
Fragments arranged by size:
2177 2042 1230 648 359 205 159
MspA1I CmG'CkG
Cuts at: 1606 2160 3372 4236 4913 6061 6306 1606
Size: 554 1212 864 677 1148 245 2120
Fragments arranged by size:
2120 1212 1148 864 677 554 245
NaeI GCC'GGC
Cuts at: 3472 3472
Size: 6820
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5329
Fragments arranged by size:
5329 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6820
NgoMIV G'CCGG_C
Cuts at: 3470 3470
Size: 6820
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6820
NotI GC'GGCC_GC
Cuts at: 4267 4267
Size: 6820
NsiI A_TGCA'T
Cuts at: 2468 2838 2468
Size: 370 6450
NspI r_CATG'y
Cuts at: 1132 1229 2466 2620 4178 5723 1132
Size: 97 1237 154 1558 1545 2229
Fragments arranged by size:
2229 1558 1545 1237 154 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6820
PciI A'CATG_T
Cuts at: 2616 5719 2616
Size: 3103 3717
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2545 1108
Size: 1437 5383
PfoI T'CCnGG_A
Cuts at: 3885 3885
Size: 6820
PmeI GTTT'AAAC
Cuts at: 4124 4124
Size: 6820
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4889 4921 6402 6434 4889
Size: 32 1481 32 5275
Fragments arranged by size:
5275 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2437 2437
Size: 6820
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6820
PsiI TTA'TAA
Cuts at: 4390 4390
Size: 6820
PspOMI G'GGCC_C
Cuts at: 2342 2342
Size: 6820
PstI C_TGCA'G
Cuts at: 830 2192 2243 2304 4076 4172 830
Size: 1362 51 61 1772 96 3478
Fragments arranged by size:
3478 1772 1362 96 61 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6820
PvuII CAG'CTG
Cuts at: 2160 3372 2160
Size: 1212 5608
SalI G'TCGA_C
Cuts at: 1936 4162 1936
Size: 2226 4594
SapI GCTCTTCn'nnn_
Cuts at: 3602 3602
Size: 6820
SbfI CC_TGCA'GG
Cuts at: 2243 4172 2243
Size: 1929 4891
ScaI AGT'ACT
Cuts at: 5068 6780 5068
Size: 1712 5108
SexAI A'CCwGG_T
Cuts at: 2751 2751
Size: 6820
SfcI C'TryA_G
Cuts at: 826 1045 2188 2239 2300 3050 4072 4168 5303
Size: 219 1143 51 61 750 1022 96 1135
Cuts at: 5303 5984 6175 826
Size: 681 191 1471
Fragments arranged by size:
1471 1143 1135 1022 750 681 219 191
96 61 51
SfiI GGCCn_nnn'nGGCC
Cuts at: 2277 2277
Size: 6820
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6820
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6820
SmaI CCC'GGG
Cuts at: 1872 4147 1872
Size: 2275 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2282 2720 3102 4080 4924 5825
Size: 197 926 339 438 382 978 844 901
Cuts at: 5825 6087 820
Size: 262 1553
Fragments arranged by size:
1553 978 926 901 844 438 382 339
262 197
SnaBI TAC'GTA
Cuts at: 493 4611 493
Size: 4118 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6820
SphI G_CATG'C
Cuts at: 1132 4178 1132
Size: 3046 3774
SspI AAT'ATT
Cuts at: 5 52 3067 4744 5
Size: 47 3015 1677 2081
Fragments arranged by size:
3015 2081 1677 47
StuI AGG'CCT
Cuts at: 1517 4078 1517
Size: 2561 4259
StyI C'CwwG_G
Cuts at: 513 1065 2004 2444 4259 513
Size: 552 939 440 1815 3074
Fragments arranged by size:
3074 1815 939 552 440
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 3866 4847 4864 5017 5202 5647
Size: 1255 417 1869 981 17 153 185 445
Cuts at: 5647 325
Size: 1498
Fragments arranged by size:
1869 1498 1255 981 445 417 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2619 3239 3315
Size: 274 80 33 51 1443 642 620 76
Cuts at: 3315 5066 6778 96
Size: 1751 1712 138
Fragments arranged by size:
1751 1712 1443 642 620 274 138 80
76 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3207 3239 6780 6812 3207
Size: 32 3541 32 3215
Fragments arranged by size:
3541 3215 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6820
XbaI T'CTAG_A
Cuts at: 4156 4156
Size: 6820
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2542 3759 2542
Size: 1217 5603
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6820
XmaI C'CCGG_G
Cuts at: 1870 4145 1870
Size: 2275 4545
XmnI GAAnn'nnTTC
Cuts at: 2528 4949 2528
Size: 2421 4399
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4628 276
Size: 53 83 186 936 3094 2468
Fragments arranged by size:
3094 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI
BstXI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI
NarI NruI PacI PmlI PspXI PsrI RsrII SacI
SacII SanDI SfoI SrfI SwaI