KLHL28 ORF clone pFN21AE4150

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Halotag ORF Clone pFN21AE4150

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Clone info.

Species

human

Clone Name

pFN21AE4150 Sequence (with vector) Restriction Map

Product ID

FHC11370

ORF Sequence

DNA sequence (1755 bp)
Protein sequence (585 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4150

Symbol

KLHL28

Alias : BTBD5

Description

kelch-like family member 28

Original Clone ID

cp94150

Note

We obtained this clone using the E.coli strain CopyCutter EPI400.

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1755 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 585 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS76675.1 0 100.0 KLHL28 CCDS9680.1 0 100.0 KLHL28 CCDS1310.1 2.3e-97 42.7 KLHL20 CCDS30550.1 3.9e-91 42.2 KLHL17 CCDS4192.1 3.7e-84 38.7 KLHL3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013069 39 145 PF00651 BTB/POZ IPR011705 151 251 PF07707 BTB/Kelch-associated IPR006652 334 386 PF01344 Kelch repeat type 1 IPR006652 390 433 PF01344 Kelch repeat type 1 IPR006652 437 479 PF01344 Kelch repeat type 1 IPR006652 483 526 PF01344 Kelch repeat type 1 IPR006652 529 567 PF01344 Kelch repeat type 1 HMMSmart IPR000210 49 146 SM00225 BTB/POZ-like IPR011705 151 253 SM00875 BTB/Kelch-associated IPR006652 298 345 SM00612 Kelch repeat type 1 IPR006652 346 400 SM00612 Kelch repeat type 1 IPR006652 401 447 SM00612 Kelch repeat type 1 IPR006652 448 493 SM00612 Kelch repeat type 1 IPR006652 494 540 SM00612 Kelch repeat type 1 IPR006652 541 585 SM00612 Kelch repeat type 1 ProfileScan IPR000210 49 116 PS50097 BTB/POZ-like
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_059111 587 605775,614495 38.7 96.1 NP_001244123 555 605775,614495 40.1 90.4 NP_067646 568 614522 39.0 95.6 NP_009177 593 605774 37.9 94.9 NP_001154993 597 605774 37.9 94.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4150 Download

>KIEE4150  1755 bp
ATGCACTTCAAATGCAAGTATTCCTACCCTGCTCTGTACCAGATGGACCACACATCCCCG
ACCTACATGCTTGCTAACTTAACCCACTTGCATTCTGAACAACTTCTGCAGGGCTTGAAT
CTTCTTCGCCAACATCACGAACTCTGTGACATCATTCTTCGAGTAGGTGATGTTAAAATT
CATGCTCACAAAGTGGTACTTGCCAGCGTCAGCCCGTATTTCAAAGCTATGTTCACTGGA
AACCTTTCTGAAAAAGAGAACAGTGAGGTTGAGTTTCAATGCATTGATGAAACTGCTCTC
CAGGCCATTGTGGAGTATGCCTATACAGGGACTGTTTTTATTTCTCAGGACACAGTTGAA
TCTCTCCTGCCAGCAGCAAACCTACTCCAGATAAAACTTGTCCTGAAAGAATGTTGTGCA
TTTCTTGAAAGCCAACTTGATCCTGGTAATTGTATTGGAATTTCTCGTTTTGCAGAAACA
TATGGTTGCCGTGACCTTTATTTGGCAGCCACTAAATACATATGCCAGAATTTTGAAGCT
GTTTGCCAGACTGAAGAGTTTTTTGAGCTTACACATGCTGACTTGGATGAAATTGTTTCC
AATGACTGTTTGAATGTAGCTACCGAAGAGACTGTTTTTTATGCATTAGAGTCTTGGATC
AAGTATGATGTACAAGAACGCCAGAAATACTTAGCACAGTTACTAAACAGTGTACGATTA
CCATTGTTGAGTGTTAAGTTTCTCACTAGACTATATGAAGCAAATCATCTTATTCGTGAT
GATCGCACTTGTAAACATCTTTTGAATGAAGCCCTAAAGTACCACTTTATGCCTGAACAT
AGACTCTCTCATCAGACAGTCTTGATGACACGACCTCGCTGTGCTCCCAAAGTACTTTGT
GCAGTAGGAGGGAAATCTGGACTCTTTGCCTGTTTGGATAGTGTGGAGATGTACTTTCCT
CAGAATGACTCTTGGATTGGTTTGGCACCCCTAAACATTCCTCGCTATGAATTTGGAATA
TGCGTTTTAGACCAAAAAGTATATGTTATAGGTGGTATTGCAACTAATGTGCGTCCTGGC
GTCACTATCAGAAAACATGAAAATTCAGTGGAATGCTGGAATCCTGATACAAATACTTGG
ACTTCTCTAGAGAGAATGAATGAAAGCCGAAGTACTCTTGGAGTAGTAGTACTTGCAGGA
GAACTTTATGCCTTAGGTGGTTATGATGGACAATCTTATTTACAATCTGTAGAGAAGTAC
ATTCCCAAAATAAGAAAATGGCAACCTGTGGCACCAATGACGACAACAAGAAGTTGTTTT
GCTGCAGCGGTATTGGATGGAATGATATATGCCATTGGTGGGTATGGTCCTGCCCACATG
AACAGTGTGGAGCGTTATGATCCAAGTAAGGACTCCTGGGAGATGGTTGCATCCATGGCA
GATAAAAGGATTCACTTTGGCGTGGGTGTCATGCTAGGCTTTATTTTTGTGGTGGGTGGA
CATAATGGAGTCTCACATTTGTCCAGCATTGAAAGATACGATCCTCATCAAAATCAGTGG
ACTGTGTGTAGACCAATGAAAGAACCTAGAACAGGAGTTGGTGCTGCTGTAATCGATAAC
TACCTTTATGTCGTCGGTGGTCACTCAGGGTCTTCCTATCTGAATACAGTGCAGAAATAT
GATCCTATCTCAGATACGTGGCTGGATTCAGCTGGCATGATATACTGTCGCTGCAACTTT
GGGTTAACTGCACTT

ORF Protein

Cloned ORF protein sequence for pFN21AE4150 Download

>KIEE4150  585 aa
MHFKCKYSYPALYQMDHTSPTYMLANLTHLHSEQLLQGLNLLRQHHELCDIILRVGDVKI
HAHKVVLASVSPYFKAMFTGNLSEKENSEVEFQCIDETALQAIVEYAYTGTVFISQDTVE
SLLPAANLLQIKLVLKECCAFLESQLDPGNCIGISRFAETYGCRDLYLAATKYICQNFEA
VCQTEEFFELTHADLDEIVSNDCLNVATEETVFYALESWIKYDVQERQKYLAQLLNSVRL
PLLSVKFLTRLYEANHLIRDDRTCKHLLNEALKYHFMPEHRLSHQTVLMTRPRCAPKVLC
AVGGKSGLFACLDSVEMYFPQNDSWIGLAPLNIPRYEFGICVLDQKVYVIGGIATNVRPG
VTIRKHENSVECWNPDTNTWTSLERMNESRSTLGVVVLAGELYALGGYDGQSYLQSVEKY
IPKIRKWQPVAPMTTTRSCFAAAVLDGMIYAIGGYGPAHMNSVERYDPSKDSWEMVASMA
DKRIHFGVGVMLGFIFVVGGHNGVSHLSSIERYDPHQNQWTVCRPMKEPRTGVGAAVIDN
YLYVVGGHSGSSYLNTVQKYDPISDTWLDSAGMIYCRCNFGLTAL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4150 Download

>pFN21AE4150  6460 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCACTTCAAATGCAAGTATTCCTACCCTGCTCT
GTACCAGATGGACCACACATCCCCGACCTACATGCTTGCTAACTTAACCCACTTGCATTC
TGAACAACTTCTGCAGGGCTTGAATCTTCTTCGCCAACATCACGAACTCTGTGACATCAT
TCTTCGAGTAGGTGATGTTAAAATTCATGCTCACAAAGTGGTACTTGCCAGCGTCAGCCC
GTATTTCAAAGCTATGTTCACTGGAAACCTTTCTGAAAAAGAGAACAGTGAGGTTGAGTT
TCAATGCATTGATGAAACTGCTCTCCAGGCCATTGTGGAGTATGCCTATACAGGGACTGT
TTTTATTTCTCAGGACACAGTTGAATCTCTCCTGCCAGCAGCAAACCTACTCCAGATAAA
ACTTGTCCTGAAAGAATGTTGTGCATTTCTTGAAAGCCAACTTGATCCTGGTAATTGTAT
TGGAATTTCTCGTTTTGCAGAAACATATGGTTGCCGTGACCTTTATTTGGCAGCCACTAA
ATACATATGCCAGAATTTTGAAGCTGTTTGCCAGACTGAAGAGTTTTTTGAGCTTACACA
TGCTGACTTGGATGAAATTGTTTCCAATGACTGTTTGAATGTAGCTACCGAAGAGACTGT
TTTTTATGCATTAGAGTCTTGGATCAAGTATGATGTACAAGAACGCCAGAAATACTTAGC
ACAGTTACTAAACAGTGTACGATTACCATTGTTGAGTGTTAAGTTTCTCACTAGACTATA
TGAAGCAAATCATCTTATTCGTGATGATCGCACTTGTAAACATCTTTTGAATGAAGCCCT
AAAGTACCACTTTATGCCTGAACATAGACTCTCTCATCAGACAGTCTTGATGACACGACC
TCGCTGTGCTCCCAAAGTACTTTGTGCAGTAGGAGGGAAATCTGGACTCTTTGCCTGTTT
GGATAGTGTGGAGATGTACTTTCCTCAGAATGACTCTTGGATTGGTTTGGCACCCCTAAA
CATTCCTCGCTATGAATTTGGAATATGCGTTTTAGACCAAAAAGTATATGTTATAGGTGG
TATTGCAACTAATGTGCGTCCTGGCGTCACTATCAGAAAACATGAAAATTCAGTGGAATG
CTGGAATCCTGATACAAATACTTGGACTTCTCTAGAGAGAATGAATGAAAGCCGAAGTAC
TCTTGGAGTAGTAGTACTTGCAGGAGAACTTTATGCCTTAGGTGGTTATGATGGACAATC
TTATTTACAATCTGTAGAGAAGTACATTCCCAAAATAAGAAAATGGCAACCTGTGGCACC
AATGACGACAACAAGAAGTTGTTTTGCTGCAGCGGTATTGGATGGAATGATATATGCCAT
TGGTGGGTATGGTCCTGCCCACATGAACAGTGTGGAGCGTTATGATCCAAGTAAGGACTC
CTGGGAGATGGTTGCATCCATGGCAGATAAAAGGATTCACTTTGGCGTGGGTGTCATGCT
AGGCTTTATTTTTGTGGTGGGTGGACATAATGGAGTCTCACATTTGTCCAGCATTGAAAG
ATACGATCCTCATCAAAATCAGTGGACTGTGTGTAGACCAATGAAAGAACCTAGAACAGG
AGTTGGTGCTGCTGTAATCGATAACTACCTTTATGTCGTCGGTGGTCACTCAGGGTCTTC
CTATCTGAATACAGTGCAGAAATATGATCCTATCTCAGATACGTGGCTGGATTCAGCTGG
CATGATATACTGTCGCTGCAACTTTGGGTTAACTGCACTTGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT