Restriction map for
pFN21AE4150
##################################
# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsoBI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I CelII CfoI
Cfr10I CpoI DraI DraII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRI EcoRV
FauI FbaI FseI FspI HaeII
HapII HhaI HinP1I HindIII HpaII
KasI KpnI KspI MfeI MflI
MluI MluNI MroI MscI MslI
MspI MunI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I Van91I XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 1568 GTAGAC GT^[AC][GT]AC
AciI 1327 GCGG CCGC(-3/-1)
AcyI 1078 GGCGTC G[GA]^CG[CT]C
AlwNI 326 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1211 CCTTAGG CC^T[ACGT]AGG
AspHI 881 GTGCTC G[AT]GC[AT]^C
BbsI 1650 GTCTTC GAAGAC(2/6)
BcgI 59 CGACCTACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 881 GTGCTC G[AGT]GC[ACT]^C
BpuAI 1650 GTCTTC GAAGAC(2/6)
BsaAI 1695 TACGTG [CT]AC^GT[GA]
BsaBI 1540 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1078 GGCGTC G[GA]^CG[CT]C
BsiHKAI 881 GTGCTC G[AT]GC[AT]^C
BsmFI 328 GGGAC GGGAC(10/14)
Bsp1286I 881 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 669 TGTACA T^GTACA
BspDI 1612 ATCGAT AT^CGAT
BsrGI 669 TGTACA T^GTACA
BsrI 235 ACTGG ACTGG(1/-1)
Bsu36I 1211 CCTTAGG CC^T[ACGT]AGG
ClaI 1612 ATCGAT AT^CGAT
DraIII 187 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1433 CCATGG C^C[GA][CT]GG
Eco81I 1211 CCTTAGG CC^T[ACGT]AGG
EcoNI 1585 CCTAGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1433 CCATGG C^C[AT][AT]GG
HaeIII 303 GGCC GG^CC
Hin1I 1078 GGCGTC G[GA]^CG[CT]C
HincII 1743 GTTAAC GT[CT]^[GA]AC
HindII 1743 GTTAAC GT[CT]^[GA]AC
HpaI 1743 GTTAAC GTT^AAC
HphI 166 GGTGA GGTGA(8/7)
MaeII 1696 ACGT A^CGT
MamI 1540 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1433 CCATGG C^CATGG
PvuII 1709 CAGCTG CAG^CTG
SfaNI 1429 GCATC GCATC(5/9)
SspBI 669 TGTACA T^GTACA
StyI 1433 CCATGG C^C[AT][AT]GG
XbaI 1146 TCTAGA T^CTAGA
XcmI 432 CCAACTTGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Asp700I 97 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
239 GAAACCTTTC
AvaII 45 GGACC G^G[AT]CC
1366 GGTCC
BanI 984 GGCACC G^G[CT][GA]CC
1290 GGCACC
BpmI 298 CTCCAG CTGGAG(16/14)
385 CTCCAG
BsaJI 1415 CCTGGG C^C[ACGT][ACGT]GG
1433 CCATGG
BsmAI 628 GAGAC GTCTC(1/5)
1510 GTCTC
BsmI 1111 GAATGC GAATGC(1/-1)
90 GCATTC
Cfr13I 45 GGACC G^G[ACGT]CC
1366 GGTCC
EarI 553 GAAGAG CTCTTC(1/4)
625 GAAGAG
EcoT22I 279 ATGCAT ATGCA^T
641 ATGCAT
Ksp632I 553 GAAGAG CTCTTC(1/4)
625 GAAGAG
MspA1I 1325 CAGCGG C[AC]G^C[GT]G
1709 CAGCTG
MwoI 295 GCTCTCCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1429 GCATCCATGGC
NdeI 479 CATATG CA^TATG
519 CATATG
NlaIV 984 GGCACC GG[ACGT]^[ACGT]CC
1290 GGCACC
NsiI 279 ATGCAT ATGCA^T
641 ATGCAT
NspI 65 ACATGC [GA]CATG^[CT]
573 ACATGC
PstI 106 CTGCAG CTGCA^G
1322 CTGCAG
Sau96I 45 GGACC G^G[ACGT]CC
1366 GGTCC
TaqI 159 TCGA T^CGA
1613 TCGA
TthHB8I 159 TCGA T^CGA
1613 TCGA
XmnI 97 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
239 GAAACCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BfaI BsgI BsiYI BslI
BssKI BstF5I BstNI Cac8I DdeI
DpnII EcoRII Fnu4HI FokI HgaI
HinfI ItaI MaeI MaeIII MboI
MboII MnlI MseI MvaI NdeII
NlaIII PleI RsaI Sau3AI ScaI
ScrFI SfcI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI