(Circular) (Six-base) MAPSORT of: pFN21AE4150.seq Check: 7215 from: 1 to: 6460 pFN21AE4150 6460 bp With 182 enzymes: SgfI * March 28, 2012 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4270 278 Size: 53 83 186 936 2734 2468 Fragments arranged by size: 2734 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3574 3803 1937 Size: 1637 229 4594 Fragments arranged by size: 4594 1637 229 Acc65I G'GTAC_C Cuts at: 3781 3781 Size: 6460 AclI AA'CG_TT Cuts at: 1563 4587 4960 1563 Size: 3024 373 3063 Fragments arranged by size: 3063 3024 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2577 4523 5907 6175 1632 Size: 945 1946 1384 268 1917 Fragments arranged by size: 1946 1917 1384 945 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6263 AflIII A'CryG_T Cuts at: 1203 5359 6336 1203 Size: 4156 977 1327 Fragments arranged by size: 4156 1327 977 AgeI A'CCGG_T Cuts at: 5291 5291 Size: 6460 AhdI GACnn_n'nnGTC Cuts at: 5189 5189 Size: 6460 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6460 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6460 AlwNI CAG_nnn'CTG Cuts at: 2336 4203 5775 2336 Size: 1867 1572 3021 Fragments arranged by size: 3021 1867 1572 ApaLI G'TGCA_C Cuts at: 4517 5673 4517 Size: 1156 5304 ApoI r'AATT_y Cuts at: 1493 2181 2463 2533 3014 3106 3769 3995 4147 Size: 688 282 70 481 92 663 226 152 Cuts at: 4147 6256 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 688 663 481 282 226 152 92 70 AseI AT'TA_AT Cuts at: 160 1032 5014 160 Size: 872 3982 1606 Fragments arranged by size: 3982 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6460 AvaI C'yCGr_G Cuts at: 1870 1943 3785 1870 Size: 73 1842 4545 Fragments arranged by size: 4545 1842 73 BamHI G'GATC_C Cuts at: 3790 4157 3790 Size: 367 6093 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2989 3295 3781 5236 618 Size: 325 217 627 1202 306 486 1455 1842 Fragments arranged by size: 1842 1455 1202 627 486 325 306 217 BanII G_rGCy'C Cuts at: 1391 3779 1391 Size: 2388 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3648 928 Size: 971 1749 3740 Fragments arranged by size: 3740 1749 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2053 2087 4651 4685 1903 Size: 34 50 34 32 34 2564 34 3678 Fragments arranged by size: 3678 2564 50 34 34 34 34 32 BciVI GTATCCnnnnn_n' Cuts at: 4352 5562 4352 Size: 1210 5250 BclI T'GATC_A Cuts at: 1548 1548 Size: 6460 BfrBI ATG'CAT Cuts at: 2286 2648 2286 Size: 362 6098 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5071 136 Size: 107 122 71 631 605 3399 1525 Fragments arranged by size: 3399 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6455 6455 Size: 6460 BlpI GC'TnA_GC Cuts at: 3877 3877 Size: 6460 Bme1580I G_kGCm'C Cuts at: 1790 4521 5677 1790 Size: 2731 1156 2573 Fragments arranged by size: 2731 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6460 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4191 5149 442 Size: 416 53 83 399 24 2774 958 1753 Fragments arranged by size: 2774 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6460 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2288 2375 5120 1239 Size: 1049 87 2745 2579 Fragments arranged by size: 2745 2579 1049 87 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4585 5450 5748 4585 Size: 865 298 5297 Fragments arranged by size: 5297 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5123 882 Size: 617 3624 2219 Fragments arranged by size: 3624 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3702 4251 493 Size: 711 2498 549 2702 Fragments arranged by size: 2702 2498 711 549 BsaBI GATnn'nnATC Cuts at: 3549 6454 3549 Size: 2905 3555 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3084 4267 4649 5284 Size: 53 83 186 936 1551 1183 382 635 Cuts at: 5284 275 Size: 1451 Fragments arranged by size: 1551 1451 1183 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4893 5291 5565 5712 6123 4893 Size: 398 274 147 411 5230 Fragments arranged by size: 5230 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3177 3207 1178 Size: 30 165 30 1774 30 4431 Fragments arranged by size: 4431 1774 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6460 BseYI C'CCAG_C Cuts at: 5663 6159 5663 Size: 496 5964 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2925 3695 3738 1163 Size: 573 1189 770 43 3885 Fragments arranged by size: 3885 1189 770 573 43 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3910 4671 5699 1540 Size: 275 186 1909 761 1028 2301 Fragments arranged by size: 2301 1909 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2890 4521 4606 5677 2890 Size: 1631 85 1071 3673 Fragments arranged by size: 3673 1631 1071 85 BsmI GAATG_Cn' Cuts at: 2095 3122 3971 4064 2095 Size: 1027 849 93 4491 Fragments arranged by size: 4491 1027 849 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2890 3779 4521 4606 5677 1391 Size: 399 1100 889 742 85 1071 2174 Fragments arranged by size: 2174 1100 1071 889 742 399 85 BspEI T'CCGG_A Cuts at: 6123 6123 Size: 6460 BspHI T'CATG_A Cuts at: 4349 6079 4349 Size: 1730 4730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3815 844 Size: 2971 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4347 1394 Size: 429 2524 3507 Fragments arranged by size: 3507 2524 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4955 5129 65 Size: 1183 3707 174 1396 Fragments arranged by size: 3707 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5104 5291 1700 Size: 110 3294 187 2869 Fragments arranged by size: 3294 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2674 96 Size: 2578 3882 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6460 BssSI C'ACGA_G Cuts at: 4520 5532 4520 Size: 1012 5448 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6460 BstBI TT'CG_AA Cuts at: 5264 5264 Size: 6460 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6460 BstYI r'GATC_y Cuts at: 1972 3790 4157 4542 4559 6011 6097 6109 6455 Size: 1818 367 385 17 1452 86 12 346 Cuts at: 6455 1972 Size: 1977 Fragments arranged by size: 1977 1818 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3217 3217 Size: 6460 BtgI C'CryG_G Cuts at: 513 1065 3438 513 Size: 552 2373 3535 Fragments arranged by size: 3535 2373 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5721 Fragments arranged by size: 5721 683 56 BtsI GCAGTG_nn' Cuts at: 3976 4770 4790 3976 Size: 794 20 5646 Fragments arranged by size: 5646 794 20 ClaI AT'CG_AT Cuts at: 1554 3618 1554 Size: 2064 4396 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3542 3577 529 Size: 35 2978 35 3412 Fragments arranged by size: 3412 2978 35 35 DraI TTT'AAA Cuts at: 3764 3764 Size: 6460 DraIII CAC_nnn'GTG Cuts at: 2197 2197 Size: 6460 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5467 809 Size: 507 4151 1802 Fragments arranged by size: 4151 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3907 4796 6425 Size: 54 1425 50 275 18 2077 889 1629 Cuts at: 6425 8 Size: 43 Fragments arranged by size: 2077 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3907 1537 Size: 275 2095 4090 Fragments arranged by size: 4090 2095 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2553 2625 4390 1353 Size: 1200 72 1765 3423 Fragments arranged by size: 3423 1765 1200 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5047 5421 5567 387 Size: 1064 138 3458 374 146 1280 Fragments arranged by size: 3458 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2288 2375 2577 4523 5120 5907 6175 Size: 393 656 87 202 1946 597 787 268 Cuts at: 6175 1239 Size: 1524 Fragments arranged by size: 1946 1524 787 656 597 393 268 202 87 EcoNI CCTnn'n_nnAGG Cuts at: 3594 3594 Size: 6460 EcoRI G'AATT_C Cuts at: 3769 4147 3769 Size: 378 6082 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2219 2845 2219 Size: 626 5834 FspI TGC'GCA Cuts at: 4966 4966 Size: 6460 HaeII r_GCGC'y Cuts at: 1678 5607 1678 Size: 3929 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2042 2074 2863 2895 3221 Size: 32 135 32 470 32 789 32 326 Cuts at: 3221 3253 3677 3709 3715 3747 5101 5133 5175 Size: 32 424 32 6 32 1354 32 42 Cuts at: 5175 5207 1373 Size: 32 2626 Fragments arranged by size: 2626 1354 789 470 424 326 135 42 32 32 32 32 32 32 32 32 32 6 HincII GTy'rAC Cuts at: 669 1657 1938 3750 3804 4050 669 Size: 988 281 1812 54 246 3079 Fragments arranged by size: 3079 1812 988 281 246 54 HindIII A'AGCT_T Cuts at: 748 748 Size: 6460 HpaI GTT'AAC Cuts at: 3750 4050 3750 Size: 300 6160 KpnI G_GTAC'C Cuts at: 3785 3785 Size: 6460 MfeI C'AATT_G Cuts at: 4059 4059 Size: 6460 MluI A'CGCG_T Cuts at: 6336 6336 Size: 6460 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5574 5758 1305 Size: 4269 184 2007 Fragments arranged by size: 4269 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4638 Fragments arranged by size: 4638 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4418 4777 4936 518 Size: 648 3252 359 159 2042 Fragments arranged by size: 3252 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3332 3716 3876 4553 5701 5946 1606 Size: 1726 384 160 677 1148 245 2120 Fragments arranged by size: 2120 1726 1148 677 384 245 160 NcoI C'CATG_G Cuts at: 513 1065 3438 513 Size: 552 2373 3535 Fragments arranged by size: 3535 2373 552 NdeI CA'TA_TG Cuts at: 387 2485 2525 387 Size: 2098 40 4322 Fragments arranged by size: 4322 2098 40 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6460 NotI GC'GGCC_GC Cuts at: 3907 3907 Size: 6460 NsiI A_TGCA'T Cuts at: 2288 2650 2288 Size: 362 6098 NspI r_CATG'y Cuts at: 1132 1229 2074 2582 3818 5363 1132 Size: 97 845 508 1236 1545 2229 Fragments arranged by size: 2229 1545 1236 845 508 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6460 PciI A'CATG_T Cuts at: 5359 5359 Size: 6460 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6460 PmeI GTTT'AAAC Cuts at: 3764 3764 Size: 6460 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2832 2864 4529 4561 6042 6074 2832 Size: 32 1665 32 1481 32 3218 Fragments arranged by size: 3218 1665 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6460 PsiI TTA'TAA Cuts at: 4030 4030 Size: 6460 PstI C_TGCA'G Cuts at: 830 2115 3331 3812 830 Size: 1285 1216 481 3478 Fragments arranged by size: 3478 1285 1216 481 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6460 PvuII CAG'CTG Cuts at: 3716 3716 Size: 6460 SalI G'TCGA_C Cuts at: 1936 3802 1936 Size: 1866 4594 SbfI CC_TGCA'GG Cuts at: 3812 3812 Size: 6460 ScaI AGT'ACT Cuts at: 2898 3178 3195 4708 6420 2898 Size: 280 17 1513 1712 2938 Fragments arranged by size: 2938 1712 1513 280 17 SfcI C'TryA_G Cuts at: 826 1045 2111 3252 3327 3808 4943 5624 5815 Size: 219 1066 1141 75 481 1135 681 191 Cuts at: 5815 826 Size: 1471 Fragments arranged by size: 1471 1141 1135 1066 681 481 219 191 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6460 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6460 SmaI CCC'GGG Cuts at: 1872 3787 1872 Size: 1915 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4564 5465 5727 820 Size: 197 926 2621 901 262 1553 Fragments arranged by size: 2621 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4251 493 Size: 3758 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6460 SphI G_CATG'C Cuts at: 1132 3818 1132 Size: 2686 3774 SspI AAT'ATT Cuts at: 5 52 4384 5 Size: 47 4332 2081 Fragments arranged by size: 4332 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6460 StyI C'CwwG_G Cuts at: 513 1065 3438 3899 513 Size: 552 2373 461 3074 Fragments arranged by size: 3074 2373 552 461 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3458 3488 4487 4504 4657 4842 5287 Size: 1255 1878 30 999 17 153 185 445 Cuts at: 5287 325 Size: 1498 Fragments arranged by size: 1878 1498 1255 999 445 185 153 30 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2674 2896 2955 Size: 274 80 33 51 1443 697 222 59 Cuts at: 2955 3176 3193 3261 4706 6418 96 Size: 221 17 68 1445 1712 138 Fragments arranged by size: 1712 1445 1443 697 274 222 221 138 80 68 59 51 33 17 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6420 6452 6420 Size: 32 6428 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6460 XbaI T'CTAG_A Cuts at: 3151 3796 3151 Size: 645 5815 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2444 2444 Size: 6460 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6460 XmaI C'CCGG_G Cuts at: 1870 3785 1870 Size: 1915 4545 XmnI GAAnn'nnTTC Cuts at: 2106 2248 4589 2106 Size: 142 2341 3977 Fragments arranged by size: 3977 2341 142 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4268 276 Size: 53 83 186 936 2734 2468 Fragments arranged by size: 2734 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI