Restriction map for
pFN21AE4150
(Linear) (Six-base) MAPSORT of: KIEE4150.seq Check: 8879 from: 1 to: 1755
KIEE4150.orf
With 182 enzymes: SgfI *
March 22, 2012 17:28 ..
AccI GT'mk_AC
Cuts at: 0 1569 1755
Size: 1569 186
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 1755
Size: 572 1183
AlwNI CAG_nnn'CTG
Cuts at: 0 331 1755
Size: 331 1424
ApoI r'AATT_y
Cuts at: 0 176 458 528 1009 1101 1755
Size: 176 282 70 481 92 654
Fragments arranged by size:
654 481 282 176 92 70
BanI G'GyrC_C
Cuts at: 0 984 1290 1755
Size: 984 306 465
Fragments arranged by size:
984 465 306
BbsI GAAGACnn'nnnn_
Cuts at: 0 1643 1755
Size: 1643 112
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 48 82 1755
Size: 48 34 1673
Fragments arranged by size:
1673 48 34
BfrBI ATG'CAT
Cuts at: 0 281 643 1755
Size: 281 362 1112
Fragments arranged by size:
1112 362 281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 370 1755
Size: 283 87 1385
Fragments arranged by size:
1385 283 87
BsaAI yAC'GTr
Cuts at: 0 1697 1755
Size: 1697 58
BsaBI GATnn'nnATC
Cuts at: 0 1544 1755
Size: 1544 211
BsaHI Gr'CG_yC
Cuts at: 0 1079 1755
Size: 1079 676
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1172 1202 1755
Size: 1172 30 553
Fragments arranged by size:
1172 553 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 920 1690 1733 1755
Size: 920 770 43 22
Fragments arranged by size:
920 770 43 22
BsiHKAI G_wGCw'C
Cuts at: 0 885 1755
Size: 885 870
BsmI GAATG_Cn'
Cuts at: 0 90 1117 1755
Size: 90 1027 638
Fragments arranged by size:
1027 638 90
Bsp1286I G_dGCh'C
Cuts at: 0 885 1755
Size: 885 870
BsrGI T'GTAC_A
Cuts at: 0 669 1755
Size: 669 1086
Bsu36I CC'TnA_GG
Cuts at: 0 1212 1755
Size: 1212 543
BtgI C'CryG_G
Cuts at: 0 1433 1755
Size: 1433 322
ClaI AT'CG_AT
Cuts at: 0 1613 1755
Size: 1613 142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1537 1572 1755
Size: 1537 35 183
Fragments arranged by size:
1537 183 35
DraIII CAC_nnn'GTG
Cuts at: 0 192 1755
Size: 192 1563
EarI CTCTTCn'nnn_
Cuts at: 0 548 620 1755
Size: 548 72 1135
Fragments arranged by size:
1135 548 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 370 572 1755
Size: 283 87 202 1183
Fragments arranged by size:
1183 283 202 87
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1589 1755
Size: 1589 166
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 214 840 1755
Size: 214 626 915
Fragments arranged by size:
915 626 214
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 37 69 858 890 1216 1248 1672 1704
Size: 37 32 789 32 326 32 424 32
Cuts at: 1704 1710 1742 1755
Size: 6 32 13
Fragments arranged by size:
789 424 326 37 32 32 32 32
32 13 6
HincII GTy'rAC
Cuts at: 0 1745 1755
Size: 1745 10
HpaI GTT'AAC
Cuts at: 0 1745 1755
Size: 1745 10
MspA1I CmG'CkG
Cuts at: 0 1327 1711 1755
Size: 1327 384 44
Fragments arranged by size:
1327 384 44
NcoI C'CATG_G
Cuts at: 0 1433 1755
Size: 1433 322
NdeI CA'TA_TG
Cuts at: 0 480 520 1755
Size: 480 40 1235
Fragments arranged by size:
1235 480 40
NsiI A_TGCA'T
Cuts at: 0 283 645 1755
Size: 283 362 1110
Fragments arranged by size:
1110 362 283
NspI r_CATG'y
Cuts at: 0 69 577 1755
Size: 69 508 1178
Fragments arranged by size:
1178 508 69
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 827 859 1755
Size: 827 32 896
Fragments arranged by size:
896 827 32
PstI C_TGCA'G
Cuts at: 0 110 1326 1755
Size: 110 1216 429
Fragments arranged by size:
1216 429 110
PvuII CAG'CTG
Cuts at: 0 1711 1755
Size: 1711 44
ScaI AGT'ACT
Cuts at: 0 893 1173 1190 1755
Size: 893 280 17 565
Fragments arranged by size:
893 565 280 17
SfcI C'TryA_G
Cuts at: 0 106 1247 1322 1755
Size: 106 1141 75 433
Fragments arranged by size:
1141 433 106 75
StyI C'CwwG_G
Cuts at: 0 1433 1755
Size: 1433 322
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1453 1483 1755
Size: 1453 30 272
Fragments arranged by size:
1453 272 30
TatI w'GTAC_w
Cuts at: 0 669 891 950 1171 1188 1256 1755
Size: 669 222 59 221 17 68 499
Fragments arranged by size:
669 499 222 221 68 59 17
XbaI T'CTAG_A
Cuts at: 0 1146 1755
Size: 1146 609
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 439 1755
Size: 439 1316
XmnI GAAnn'nnTTC
Cuts at: 0 101 243 1755
Size: 101 142 1512
Fragments arranged by size:
1512 142 101
Enzymes that do cut:
AccI AcuI AlwNI ApoI BanI BbsI BcgI BfrBI
BpmI BsaAI BsaBI BsaHI BsaXI BsgI BsiHKAI BsmI
Bsp1286I BsrGI Bsu36I BtgI ClaI CspCI DraIII EarI
Eco57MI EcoNI FalI Hin4I HincII HpaI MspA1I NcoI
NdeI NsiI NspI PpiI PstI PvuII ScaI SfcI
StyI TaqII TatI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaWI BseRI BseYI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgZI BtsI DraI DrdI EaeI EagI EciI
EcoICRI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
NaeI NarI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PfoI PmeI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XhoI XmaI ZraI