KLHL30 ORF clone pFN21AE4145

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE4145

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Clone info.

Species

human

Clone Name

pFN21AE4145 Sequence (with vector) Restriction Map

Product ID

FHC30938

ORF Sequence

DNA sequence (1734 bp)
Protein sequence (578 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4145

Symbol

KLHL30

Description

kelch-like family member 30

Original Clone ID

cp94145

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1734 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 578 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS46555.2 0 99.7 KLHL30 CCDS30575.1 5.8e-70 38.4 KLHL21 CCDS3246.1 1.3e-47 30.5 KLHL24 CCDS34094.1 1.9e-44 29.2 KLHL2 CCDS54815.1 1.9e-44 29.2 KLHL2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013069 23 130 PF00651 BTB/POZ IPR011705 135 235 PF07707 BTB/Kelch-associated IPR006652 318 363 PF01344 Kelch repeat type 1 IPR006652 366 407 PF01344 Kelch repeat type 1 IPR006652 507 545 PF01344 Kelch repeat type 1 HMMSmart IPR000210 33 130 SM00225 BTB/POZ-like IPR011705 135 237 SM00875 BTB/Kelch-associated IPR006652 327 377 SM00612 Kelch repeat type 1 IPR006652 378 422 SM00612 Kelch repeat type 1 IPR006652 472 513 SM00612 Kelch repeat type 1 IPR006652 514 564 SM00612 Kelch repeat type 1 ProfileScan IPR000210 33 100 PS50097 BTB/POZ-like
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055666 597 616262 38.4 96.0 NP_001311238 500 616262 38.5 85.3 XP_016862149 600 611295 30.5 94.8 XP_011511240 600 611295 30.5 94.8 NP_060114 600 611295 30.5 94.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4145 Download

>KIEE4145  1734 bp
ATGGTGCGGAACGTGGATGACCTGGATTTCCACCTGCCCTCGCATGCCCAGGACATGCTG
GATGGCCTGCAGCGCCTGCGCTCTCAGCCCAAGCTGGCCGACGTCACACTGCTGGTGGGC
GGCCGGGAGCTGCCATGCCACCGCAGCCTCCTGGCGCTCAGCAGCCCCTACTTCCATGCC
ATGTTTGCGGGTGACTTCGCCGAGAGCTTCTCTGCGCGCGTGGAGCTGCGGGACGTGGAG
CCCGCCGTGGTGGGACAACTGGTGGACTTCGTGTACACAGGCCGGCTGACCATCACGCAG
GGCAACGTGGAGGCGCTGACACGCACGGCTGCGCGCCTGCACTTCCCCTCGGTGCAGAAG
GTCTGCGGCCGCTACCTGCAGCAGCAACTGGATGCCGCCAACTGCCTGGGCATCTGTGAG
TTCGGGGAGCAGCAAGGGCTGCTGGGCGTGGCTGCCAAGGCCTGGGCCTTCCTGCGAGAG
AACTTTGAGGCTGTGGCACGTGAGGACGAGTTCCTGCAGCTTCCCCGAGAGCGGCTGGTC
ACTTGTCTGGCCGGCGACCTGCTGCAGGTACAGCCGGAGCAAAGCCGACTCGAGGCCCTG
ATGCGCTGGGTGCGCCATGACCCGCAGGCCCGGGCCGTCCACCTGCCCGAGCTGCTCAGC
CTAGTGCACCTGGACGCCGTGCCCAGGCCCTGCGTGCAGCAACTGCTGGCCTCAGAGCCC
CTGATCCAGGAGTCAGAGGCATGCCGGGCAGCCCTGTCCCAGGGCCATGATGGGGCACCA
CTCGCCCTCCAGCAGAAGCTGGAGGAGGTCCTGGTGGTGGTGGGCGGGCAGGCGCTGGAG
GAGGAGGAGGCAGGTGAGGAGCCCACCCCCGGCCTTGGGAACTTCGCCTTCTACAACAGC
AAGGCCAAGAGGTGGATGGCACTTCCAGACTTCCCCGACTATCACAAGTGGGGTTTCTCC
CTGGCGGCCCTGAACAACAACATCTATGTCACAGGTGGCTCTCGGGGCACAAAGACAGAC
ACCTGGTCAACCACCCAGGCCTGGTGCTTCCCCCTGAAGGAGGCCTCCTGGAAGCCCGTG
GCGCCCATGCTGAAGCCCCGCACCAACCACGCCAGCGCGGCCCTCAATGGGGAGATCTAC
GTTATCGGCGGCACCACCCTGGACGTGGTGGAGGTGGAGAGCTATGACCCCTACACGGAC
AGCTGGACGCCCGTCAGCCCGGCCCTCAAATACGTCAGCAACTTCTCGGCTGCCGGCTGC
CGGGGCCGGCTCTACCTGGTGGGCTCCAGCGCCTGCAAGTACAACGCCCTGGCCCTGCAG
TGCTACAACCCTGTCACAGATGCGTGGAGTGTGATCGCCTCGCCCTTCCTGCCCAAGTAC
CTGTCCTCGCCTCGCTGTGCTGCACTGCACGGGGAGCTCTACCTCATTGGGGACAACACC
AAGAAGGTCTACGTGTACGACCCCGGGGCCAACCTGTGGCAGAAGGTGCAGTCACAGCAC
AGCCTGCATGAGAACGGCGCGCTGGTGCCACTGGGTGATGCGCTGTACGTGACGGGCGGC
CGCTGGCAGGGCATGGAAGGTGACTACCACGTGGAGATGGAGGCCTACGACACGGTTCGG
GACACCTGGACCCGCCACGGCGCCCTGCCCCGGCTCTGGCTCTACCACGGGGCCTCCACC
GTCTTCCTGGATGTCTCCAAGTGGACCCAGCCCTCCGGCCCCACCCAGGAGCAC

ORF Protein

Cloned ORF protein sequence for pFN21AE4145 Download

>KIEE4145  578 aa
MVRNVDDLDFHLPSHAQDMLDGLQRLRSQPKLADVTLLVGGRELPCHRSLLALSSPYFHA
MFAGDFAESFSARVELRDVEPAVVGQLVDFVYTGRLTITQGNVEALTRTAARLHFPSVQK
VCGRYLQQQLDAANCLGICEFGEQQGLLGVAAKAWAFLRENFEAVAREDEFLQLPRERLV
TCLAGDLLQVQPEQSRLEALMRWVRHDPQARAVHLPELLSLVHLDAVPRPCVQQLLASEP
LIQESEACRAALSQGHDGAPLALQQKLEEVLVVVGGQALEEEEAGEEPTPGLGNFAFYNS
KAKRWMALPDFPDYHKWGFSLAALNNNIYVTGGSRGTKTDTWSTTQAWCFPLKEASWKPV
APMLKPRTNHASAALNGEIYVIGGTTLDVVEVESYDPYTDSWTPVSPALKYVSNFSAAGC
RGRLYLVGSSACKYNALALQCYNPVTDAWSVIASPFLPKYLSSPRCAALHGELYLIGDNT
KKVYVYDPGANLWQKVQSQHSLHENGALVPLGDALYVTGGRWQGMEGDYHVEMEAYDTVR
DTWTRHGALPRLWLYHGASTVFLDVSKWTQPSGPTQEH

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4145 Download

>pFN21AE4145  6439 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGTGCGGAACGTGGATGACCTGGATTTCCACCT
GCCCTCGCATGCCCAGGACATGCTGGATGGCCTGCAGCGCCTGCGCTCTCAGCCCAAGCT
GGCCGACGTCACACTGCTGGTGGGCGGCCGGGAGCTGCCATGCCACCGCAGCCTCCTGGC
GCTCAGCAGCCCCTACTTCCATGCCATGTTTGCGGGTGACTTCGCCGAGAGCTTCTCTGC
GCGCGTGGAGCTGCGGGACGTGGAGCCCGCCGTGGTGGGACAACTGGTGGACTTCGTGTA
CACAGGCCGGCTGACCATCACGCAGGGCAACGTGGAGGCGCTGACACGCACGGCTGCGCG
CCTGCACTTCCCCTCGGTGCAGAAGGTCTGCGGCCGCTACCTGCAGCAGCAACTGGATGC
CGCCAACTGCCTGGGCATCTGTGAGTTCGGGGAGCAGCAAGGGCTGCTGGGCGTGGCTGC
CAAGGCCTGGGCCTTCCTGCGAGAGAACTTTGAGGCTGTGGCACGTGAGGACGAGTTCCT
GCAGCTTCCCCGAGAGCGGCTGGTCACTTGTCTGGCCGGCGACCTGCTGCAGGTACAGCC
GGAGCAAAGCCGACTCGAGGCCCTGATGCGCTGGGTGCGCCATGACCCGCAGGCCCGGGC
CGTCCACCTGCCCGAGCTGCTCAGCCTAGTGCACCTGGACGCCGTGCCCAGGCCCTGCGT
GCAGCAACTGCTGGCCTCAGAGCCCCTGATCCAGGAGTCAGAGGCATGCCGGGCAGCCCT
GTCCCAGGGCCATGATGGGGCACCACTCGCCCTCCAGCAGAAGCTGGAGGAGGTCCTGGT
GGTGGTGGGCGGGCAGGCGCTGGAGGAGGAGGAGGCAGGTGAGGAGCCCACCCCCGGCCT
TGGGAACTTCGCCTTCTACAACAGCAAGGCCAAGAGGTGGATGGCACTTCCAGACTTCCC
CGACTATCACAAGTGGGGTTTCTCCCTGGCGGCCCTGAACAACAACATCTATGTCACAGG
TGGCTCTCGGGGCACAAAGACAGACACCTGGTCAACCACCCAGGCCTGGTGCTTCCCCCT
GAAGGAGGCCTCCTGGAAGCCCGTGGCGCCCATGCTGAAGCCCCGCACCAACCACGCCAG
CGCGGCCCTCAATGGGGAGATCTACGTTATCGGCGGCACCACCCTGGACGTGGTGGAGGT
GGAGAGCTATGACCCCTACACGGACAGCTGGACGCCCGTCAGCCCGGCCCTCAAATACGT
CAGCAACTTCTCGGCTGCCGGCTGCCGGGGCCGGCTCTACCTGGTGGGCTCCAGCGCCTG
CAAGTACAACGCCCTGGCCCTGCAGTGCTACAACCCTGTCACAGATGCGTGGAGTGTGAT
CGCCTCGCCCTTCCTGCCCAAGTACCTGTCCTCGCCTCGCTGTGCTGCACTGCACGGGGA
GCTCTACCTCATTGGGGACAACACCAAGAAGGTCTACGTGTACGACCCCGGGGCCAACCT
GTGGCAGAAGGTGCAGTCACAGCACAGCCTGCATGAGAACGGCGCGCTGGTGCCACTGGG
TGATGCGCTGTACGTGACGGGCGGCCGCTGGCAGGGCATGGAAGGTGACTACCACGTGGA
GATGGAGGCCTACGACACGGTTCGGGACACCTGGACCCGCCACGGCGCCCTGCCCCGGCT
CTGGCTCTACCACGGGGCCTCCACCGTCTTCCTGGATGTCTCCAAGTGGACCCAGCCCTC
CGGCCCCACCCAGGAGCACGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT