(Circular) (Six-base) MAPSORT of: pFN21AE4145.seq Check: 3552 from: 1 to: 6439 pFN21AE4145 6439 bp With 182 enzymes: SgfI * March 28, 2012 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2046 2655 2846 2046 Size: 609 191 5639 Fragments arranged by size: 5639 609 191 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2109 4249 278 Size: 53 83 186 936 573 2140 2468 Fragments arranged by size: 2468 2140 936 573 186 83 53 AccI GT'mk_AC Cuts at: 1937 3453 3782 1937 Size: 1516 329 4594 Fragments arranged by size: 4594 1516 329 Acc65I G'GTAC_C Cuts at: 3760 3760 Size: 6439 AclI AA'CG_TT Cuts at: 1563 4566 4939 1563 Size: 3003 373 3063 Fragments arranged by size: 3063 3003 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3080 3116 4502 5886 6154 1632 Size: 1448 36 1386 1384 268 1917 Fragments arranged by size: 1917 1448 1386 1384 268 36 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6242 AflIII A'CryG_T Cuts at: 1203 3456 5338 6315 1203 Size: 2253 1882 977 1327 Fragments arranged by size: 2253 1882 1327 977 AgeI A'CCGG_T Cuts at: 5270 5270 Size: 6439 AhdI GACnn_n'nnGTC Cuts at: 3028 5168 3028 Size: 2140 4299 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6439 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2412 1184 Size: 1228 5211 AlwNI CAG_nnn'CTG Cuts at: 2080 2392 2694 2707 2803 2839 3297 4182 5754 Size: 312 302 13 96 36 458 885 1572 Cuts at: 5754 2080 Size: 2765 Fragments arranged by size: 2765 1572 885 458 312 302 96 36 13 ApaLI G'TGCA_C Cuts at: 2669 4496 5652 2669 Size: 1827 1156 3456 Fragments arranged by size: 3456 1827 1156 ApoI r'AATT_y Cuts at: 1493 3748 3974 4126 6235 1493 Size: 2255 226 152 2109 1697 Fragments arranged by size: 2255 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4993 160 Size: 872 3961 1606 Fragments arranged by size: 3961 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6439 AvaI C'yCGr_G Cuts at: 1870 1943 2529 2594 2634 2651 3006 3467 3764 Size: 73 586 65 40 17 355 461 297 Cuts at: 3764 1870 Size: 4545 Fragments arranged by size: 4545 586 461 355 297 73 65 40 17 BamHI G'GATC_C Cuts at: 3769 4136 3769 Size: 367 6072 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2779 3085 3155 3529 3644 Size: 325 217 627 992 306 70 374 115 Cuts at: 3644 3760 5215 618 Size: 116 1455 1842 Fragments arranged by size: 1842 1455 992 627 374 325 306 217 116 115 70 BanII G_rGCy'C Cuts at: 1391 2247 2724 2868 3290 3423 3758 1391 Size: 856 477 144 422 133 335 4072 Fragments arranged by size: 4072 856 477 422 335 144 133 BbeI G_GCGC'C Cuts at: 3089 3648 3089 Size: 559 5880 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3679 928 Size: 971 1780 3688 Fragments arranged by size: 3688 1780 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4630 4664 1903 Size: 34 2693 34 3678 Fragments arranged by size: 3678 2693 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4331 5541 4331 Size: 1210 5229 BclI T'GATC_A Cuts at: 1548 1548 Size: 6439 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3122 5050 136 Size: 107 122 71 631 605 1450 1928 1525 Fragments arranged by size: 1928 1525 1450 631 605 122 107 71 BglII A'GATC_T Cuts at: 3138 6434 3138 Size: 3296 3143 BlpI GC'TnA_GC Cuts at: 2162 2660 3856 2162 Size: 498 1196 4745 Fragments arranged by size: 4745 1196 498 Bme1580I G_kGCm'C Cuts at: 1790 2673 2688 2782 3014 4500 5656 1790 Size: 883 15 94 232 1486 1156 2573 Fragments arranged by size: 2573 1486 1156 883 232 94 15 BmgBI CAC'GTC Cuts at: 1318 2239 3169 1318 Size: 921 930 4588 Fragments arranged by size: 4588 930 921 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3545 4170 5128 Size: 416 53 83 399 24 2128 625 958 Cuts at: 5128 442 Size: 1753 Fragments arranged by size: 2128 1753 958 625 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6439 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2230 2230 Size: 6439 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2777 2825 2861 3274 5099 1239 Size: 1538 48 36 413 1825 2579 Fragments arranged by size: 2579 1825 1538 413 48 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4564 5429 5727 4564 Size: 865 298 5276 Fragments arranged by size: 5276 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5102 882 Size: 617 3603 2219 Fragments arranged by size: 3603 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2504 3457 3553 3595 4230 493 Size: 711 1300 953 96 42 635 2702 Fragments arranged by size: 2702 1300 953 711 635 96 42 BsaBI GATnn'nnATC Cuts at: 6433 6433 Size: 6439 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2106 2679 3086 3212 Size: 53 83 186 936 573 573 407 126 Cuts at: 3212 3645 4246 4628 5263 275 Size: 433 601 382 635 1451 Fragments arranged by size: 1451 936 635 601 573 573 433 407 382 186 126 83 53 BsaWI w'CCGG_w Cuts at: 4872 5270 5544 5691 6102 4872 Size: 398 274 147 411 5209 Fragments arranged by size: 5209 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2775 2805 3662 3692 1178 Size: 30 165 30 1372 30 857 30 3925 Fragments arranged by size: 3925 1372 857 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2822 2858 2861 2864 2876 1186 Size: 1636 36 3 3 12 4749 Fragments arranged by size: 4749 1636 36 12 3 3 BseYI C'CCAG_C Cuts at: 2446 2610 3711 5642 6138 2446 Size: 164 1101 1931 496 2747 Fragments arranged by size: 2747 1931 1101 496 164 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2327 2378 2720 3390 3395 3512 1163 Size: 573 591 51 342 670 5 117 4090 Fragments arranged by size: 4090 670 591 573 342 117 51 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2128 2374 3565 3889 4650 5678 Size: 275 186 127 246 1191 324 761 1028 Cuts at: 5678 1540 Size: 2301 Fragments arranged by size: 2301 1191 1028 761 324 275 246 186 127 BsiHKAI G_wGCw'C Cuts at: 2673 3423 3738 4500 4585 5656 2673 Size: 750 315 762 85 1071 3456 Fragments arranged by size: 3456 1071 762 750 315 85 BsmI GAATG_Cn' Cuts at: 3950 4043 3950 Size: 93 6346 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2247 2673 2688 2724 2782 2868 3014 Size: 399 457 426 15 36 58 86 146 Cuts at: 3014 3290 3423 3738 3758 4500 4585 5656 1391 Size: 276 133 315 20 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 457 426 399 315 276 146 133 86 85 58 36 20 15 BspEI T'CCGG_A Cuts at: 6102 6102 Size: 6439 BspHI T'CATG_A Cuts at: 4328 6058 4328 Size: 1730 4709 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2046 2388 2560 2571 2655 2846 3794 844 Size: 1202 342 172 11 84 191 948 3489 Fragments arranged by size: 3489 1202 948 342 191 172 84 11 BsrBI CCG'CTC Cuts at: 1394 1823 2536 4326 1394 Size: 429 713 1790 3507 Fragments arranged by size: 3507 1790 713 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4934 5108 65 Size: 1183 3686 174 1396 Fragments arranged by size: 3686 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2286 2555 3257 3270 5083 5270 1700 Size: 110 476 269 702 13 1813 187 2869 Fragments arranged by size: 2869 1813 702 476 269 187 110 13 BsrGI T'GTAC_A Cuts at: 96 2277 96 Size: 2181 4258 BssHII G'CGCG_C Cuts at: 1925 2219 2336 3522 1925 Size: 294 117 1186 4842 Fragments arranged by size: 4842 1186 294 117 BssSI C'ACGA_G Cuts at: 4499 5511 4499 Size: 1012 5427 BstAPI GCAn_nnn'nTGC Cuts at: 794 2080 2443 2707 3490 3508 794 Size: 1286 363 264 783 18 3725 Fragments arranged by size: 3725 1286 783 363 264 18 BstBI TT'CG_AA Cuts at: 5243 5243 Size: 6439 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6439 BstXI CCAn_nnnn'nTGG Cuts at: 2410 2410 Size: 6439 BstYI r'GATC_y Cuts at: 1972 3138 3769 4136 4521 4538 5990 6076 6088 Size: 1166 631 367 385 17 1452 86 12 Cuts at: 6088 6434 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1166 631 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2250 3081 3640 3670 513 Size: 552 939 246 831 559 30 3282 Fragments arranged by size: 3282 939 831 559 552 246 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5700 Fragments arranged by size: 5700 683 56 BtsI GCAGTG_nn' Cuts at: 2111 3329 3407 3955 4749 4769 2111 Size: 1218 78 548 794 20 3781 Fragments arranged by size: 3781 1218 794 548 78 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6439 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6404 DraI TTT'AAA Cuts at: 3743 3743 Size: 6439 DraIII CAC_nnn'GTG Cuts at: 3539 3539 Size: 6439 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5446 809 Size: 507 4130 1802 Fragments arranged by size: 4130 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 2125 2371 Size: 54 1425 50 275 18 270 25 246 Cuts at: 2371 2553 3562 3886 4775 6404 8 Size: 182 1009 324 889 1629 43 Fragments arranged by size: 1629 1425 1009 889 324 275 270 246 182 54 50 43 25 18 EagI C'GGCC_G Cuts at: 1537 1812 2125 2371 3562 3886 1537 Size: 275 313 246 1191 324 4090 Fragments arranged by size: 4090 1191 324 313 275 246 EarI CTCTTCn'nnn_ Cuts at: 1353 4369 1353 Size: 3016 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5026 5400 5546 387 Size: 1064 138 3437 374 146 1280 Fragments arranged by size: 3437 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3421 3421 Size: 6439 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2777 2825 2861 3080 3116 3274 4502 Size: 393 1145 48 36 219 36 158 1228 Cuts at: 4502 5099 5886 6154 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1228 1145 787 597 393 268 219 158 48 36 36 EcoNI CCTnn'n_nnAGG Cuts at: 2567 2729 2762 3062 2567 Size: 162 33 300 5944 Fragments arranged by size: 5944 300 162 33 EcoO109I rG'GnC_Cy Cuts at: 2599 2691 2812 3675 2599 Size: 92 121 863 5363 Fragments arranged by size: 5363 863 121 92 EcoRI G'AATT_C Cuts at: 3748 4126 3748 Size: 378 6061 FspI TGC'GCA Cuts at: 4945 4945 Size: 6439 HaeII r_GCGC'y Cuts at: 1678 2080 2162 2321 2840 3089 3297 3648 5586 Size: 402 82 159 519 249 208 351 1938 Cuts at: 5586 1678 Size: 2531 Fragments arranged by size: 2531 1938 519 402 351 249 208 159 82 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3014 3046 5080 5112 5154 Size: 32 135 32 1442 32 2034 32 42 Cuts at: 5154 5186 1373 Size: 32 2626 Fragments arranged by size: 2626 2034 1442 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3033 3783 4029 669 Size: 988 281 1095 750 246 3079 Fragments arranged by size: 3079 1095 988 750 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6439 HpaI GTT'AAC Cuts at: 4029 4029 Size: 6439 KasI G'GCGC_C Cuts at: 3085 3644 3085 Size: 559 5880 KpnI G_GTAC'C Cuts at: 3764 3764 Size: 6439 MfeI C'AATT_G Cuts at: 4038 4038 Size: 6439 MluI A'CGCG_T Cuts at: 6315 6315 Size: 6439 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5553 5737 1305 Size: 4248 184 2007 Fragments arranged by size: 4248 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4617 Fragments arranged by size: 4617 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4397 4756 4915 518 Size: 648 3231 359 159 2042 Fragments arranged by size: 3231 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3207 3567 3855 4532 5680 5925 1606 Size: 1601 360 288 677 1148 245 2120 Fragments arranged by size: 2120 1601 1148 677 360 288 245 NaeI GCC'GGC Cuts at: 2288 2557 3259 3272 2288 Size: 269 702 13 5455 Fragments arranged by size: 5455 702 269 13 NarI GG'CG_CC Cuts at: 3086 3645 3086 Size: 559 5880 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4948 Fragments arranged by size: 4948 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6439 NgoMIV G'CCGG_C Cuts at: 2286 2555 3257 3270 2286 Size: 269 702 13 5455 Fragments arranged by size: 5455 702 269 13 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6439 NotI GC'GGCC_GC Cuts at: 2371 3562 3886 2371 Size: 1191 324 4924 Fragments arranged by size: 4924 1191 324 NspI r_CATG'y Cuts at: 1132 1229 2051 2062 2748 3797 5342 1132 Size: 97 822 11 686 1049 1545 2229 Fragments arranged by size: 2229 1545 1049 822 686 97 11 PasI CC'CwG_GG Cuts at: 1348 2764 1348 Size: 1416 5023 PciI A'CATG_T Cuts at: 5338 5338 Size: 6439 PflMI CCAn_nnn'nTGG Cuts at: 1108 2466 2916 3480 1108 Size: 1358 450 564 4067 Fragments arranged by size: 4067 1358 564 450 PfoI T'CCnGG_A Cuts at: 2730 3071 3690 2730 Size: 341 619 5479 Fragments arranged by size: 5479 619 341 PmeI GTTT'AAAC Cuts at: 3743 3743 Size: 6439 PmlI CAC'GTG Cuts at: 2504 3595 2504 Size: 1091 5348 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2498 2530 4508 4540 6021 6053 2498 Size: 32 1978 32 1481 32 2884 Fragments arranged by size: 2884 1978 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2812 2812 Size: 6439 PshAI GACnn'nnGTC Cuts at: 1613 3215 1613 Size: 1602 4837 PsiI TTA'TAA Cuts at: 4009 4009 Size: 6439 PspXI vC'TCGA_Gb Cuts at: 2594 2594 Size: 6439 PstI C_TGCA'G Cuts at: 830 2076 2385 2523 2571 3324 3791 830 Size: 1246 309 138 48 753 467 3478 Fragments arranged by size: 3478 1246 753 467 309 138 48 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6439 PvuII CAG'CTG Cuts at: 3207 3207 Size: 6439 SacI G_AGCT'C Cuts at: 3423 3423 Size: 6439 SalI G'TCGA_C Cuts at: 1936 3781 1936 Size: 1845 4594 SbfI CC_TGCA'GG Cuts at: 3791 3791 Size: 6439 ScaI AGT'ACT Cuts at: 4687 6399 4687 Size: 1712 4727 SexAI A'CCwGG_T Cuts at: 3026 3279 3026 Size: 253 6186 SfcI C'TryA_G Cuts at: 826 1045 2072 2381 2519 2567 3320 3787 4922 Size: 219 1027 309 138 48 753 467 1135 Cuts at: 4922 5603 5794 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1027 753 681 467 309 219 191 138 48 SfoI GGC'GCC Cuts at: 3087 3646 3087 Size: 559 5880 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6439 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6439 SmaI CCC'GGG Cuts at: 1872 2636 3469 3766 1872 Size: 764 833 297 4545 Fragments arranged by size: 4545 833 764 297 SmlI C'TyrA_G Cuts at: 820 1017 1943 2594 4543 5444 5706 820 Size: 197 926 651 1949 901 262 1553 Fragments arranged by size: 1949 1553 926 901 651 262 197 SnaBI TAC'GTA Cuts at: 493 4230 493 Size: 3737 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6439 SphI G_CATG'C Cuts at: 1132 2051 2748 3797 1132 Size: 919 697 1049 3774 Fragments arranged by size: 3774 1049 919 697 SrfI GCCC'GGGC Cuts at: 2636 2636 Size: 6439 SspI AAT'ATT Cuts at: 5 52 4363 5 Size: 47 4311 2081 Fragments arranged by size: 4311 2081 47 StuI AGG'CCT Cuts at: 1517 2465 3044 3068 3608 1517 Size: 948 579 24 540 4348 Fragments arranged by size: 4348 948 579 540 24 StyI C'CwwG_G Cuts at: 513 1065 2004 2460 2878 3878 513 Size: 552 939 456 418 1000 3074 Fragments arranged by size: 3074 1000 939 552 456 418 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2602 3053 3527 3743 4466 4483 4636 Size: 1255 1022 451 474 216 723 17 153 Cuts at: 4636 4821 5266 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1022 723 474 451 445 216 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2277 3303 4685 Size: 274 80 33 51 1443 300 1026 1382 Cuts at: 4685 6397 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1382 1026 300 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2250 2282 2775 2807 3662 3694 6399 6431 2250 Size: 32 493 32 855 32 2705 32 2258 Fragments arranged by size: 2705 2258 855 493 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6439 XbaI T'CTAG_A Cuts at: 3775 3775 Size: 6439 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2771 2917 3166 3688 2771 Size: 146 249 522 5522 Fragments arranged by size: 5522 522 249 146 XhoI C'TCGA_G Cuts at: 1943 2594 1943 Size: 651 5788 XmaI C'CCGG_G Cuts at: 1870 2634 3467 3764 1870 Size: 764 833 297 4545 Fragments arranged by size: 4545 833 764 297 XmnI GAAnn'nnTTC Cuts at: 4568 4568 Size: 6439 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2107 4247 276 Size: 53 83 186 936 573 2140 2468 Fragments arranged by size: 2468 2140 936 573 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PspOMI PsrI RsrII SacII SanDI SapI SfiI SwaI