################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AgeI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BamHI BbvCI BcgI BciVI BclI BfrI BlnI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrDI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DrdI EarI Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NcoI NdeI NheI NruI NsiI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AatII 100 GACGTC GACGT^C AccI 1447 GTCTAC GT^[AC][GT]AC AflIII 1451 ACGTGT A^C[GA][CT]GT AhdI 1018 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 664 GTGCAC G^TGCAC AlwI 723 GATCC GGATC(4/5) ApaLI 664 GTGCAC G^TGCAC AspEI 1018 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbsI 1681 GTCTTC GAAGAC(2/6) BfaI 661 CTAG C^TAG BglI 1111 GCCAGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1133 AGATCT A^GATCT BpuAI 1681 GTCTTC GAAGAC(2/6) BsmAI 1693 GTCTC GTCTC(1/5) Bsp1407I 272 TGTACA T^GTACA BsrBI 529 GAGCGG CCGCTC(-3/-3) BsrGI 272 TGTACA T^GTACA BstXI 398 CCAACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1133 AGATCT [GA]^GATC[CT] DraIII 1529 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1018 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HincII 1026 GTCAAC GT[CT]^[GA]AC HindII 1026 GTCAAC GT[CT]^[GA]AC MaeI 661 CTAG C^TAG MboII 1682 TCTTC GAAGA(8/7) MflI 1133 AGATCT [GA]^GATC[CT] PaeR7I 589 CTCGAG C^TCGAG PpuMI 806 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 1206 GACGCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1200 CAGCTG CAG^CTG SacI 1414 GAGCTC GAGCT^C SmlI 589 CTCGAG C^T[CT][GA]AG SspBI 272 TGTACA T^GTACA TaqI 590 TCGA T^CGA TthHB8I 590 TCGA T^CGA XhoI 589 CTCGAG C^TCGAG XhoII 1133 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### BbeI 1080 GGCGCC GGCGC^C 1639 GGCGCC BbrPI 497 CACGTG CAC^GTG 1588 CACGTG BlpI 156 GCTCAGC GC^T[ACGT]AGC 654 GCTCAGC Bpu1102I 156 GCTCAGC GC^T[ACGT]AGC 654 GCTCAGC CelII 156 GCTCAGC GC^T[ACGT]AGC 654 GCTCAGC EcoT14I 455 CCAAGG C^C[AT][AT]GG 873 CCTTGG HgaI 673 GACGC GACGC(5/10) 1206 GACGC HinfI 587 GACTC G^A[ACGT]TC 730 GAGTC KasI 1080 GGCGCC G^GCGCC 1639 GGCGCC MspA1I 1200 CAGCTG C[AC]G^C[GT]G 1560 CCGCTG NarI 1080 GGCGCC GG^CGCC 1639 GGCGCC NotI 365 GCGGCCGC GC^GGCCGC 1556 GCGGCCGC PleI 587 GACTC GAGTC(4/5) 730 GAGTC PmaCI 497 CACGTG CAC^GTG 1588 CACGTG PmlI 497 CACGTG CAC^GTG 1588 CACGTG SexAI 1021 ACCTGGT A^CC[AT]GGT 1274 ACCTGGT SfoI 1080 GGCGCC GGC^GCC 1639 GGCGCC SmaI 629 CCCGGG CCC^GGG 1462 CCCGGG SphI 42 GCATGC GCATG^C 739 GCATGC StyI 455 CCAAGG C^C[AT][AT]GG 873 CCTTGG XmaCI 629 CCCGGG C^CCGGG 1462 CCCGGG XmaI 629 CCCGGG C^CCGGG 1462 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI MaeII MaeIII MboI MnlI MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI TseI Tsp45I TspRI Van91I XcmI