(Linear) (Six-base) MAPSORT of: KIEE4145.seq Check: 7476 from: 1 to: 1734 KIEE4145.orf With 182 enzymes: SgfI * March 22, 2012 17:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 41 650 841 1734 Size: 41 609 191 893 Fragments arranged by size: 893 609 191 41 AatII G_ACGT'C Cuts at: 0 104 1734 Size: 104 1630 AccI GT'mk_AC Cuts at: 0 1448 1734 Size: 1448 286 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1075 1111 1734 Size: 1075 36 623 Fragments arranged by size: 1075 623 36 AflIII A'CryG_T Cuts at: 0 1451 1734 Size: 1451 283 AhdI GACnn_n'nnGTC Cuts at: 0 1023 1734 Size: 1023 711 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 407 1734 Size: 407 1327 AlwNI CAG_nnn'CTG Cuts at: 0 75 387 689 702 798 834 1292 1734 Size: 75 312 302 13 96 36 458 442 Fragments arranged by size: 458 442 312 302 96 75 36 13 ApaLI G'TGCA_C Cuts at: 0 664 1734 Size: 664 1070 AvaI C'yCGr_G Cuts at: 0 524 589 629 646 1001 1462 1734 Size: 524 65 40 17 355 461 272 Fragments arranged by size: 524 461 355 272 65 40 17 BanI G'GyrC_C Cuts at: 0 774 1080 1150 1524 1639 1734 Size: 774 306 70 374 115 95 Fragments arranged by size: 774 374 306 115 95 70 BanII G_rGCy'C Cuts at: 0 242 719 863 1285 1418 1734 Size: 242 477 144 422 133 316 Fragments arranged by size: 477 422 316 242 144 133 BbeI G_GCGC'C Cuts at: 0 1084 1643 1734 Size: 1084 559 91 Fragments arranged by size: 1084 559 91 BbsI GAAGACnn'nnnn_ Cuts at: 0 1674 1734 Size: 1674 60 BglI GCCn_nnn'nGGC Cuts at: 0 1117 1734 Size: 1117 617 BglII A'GATC_T Cuts at: 0 1133 1734 Size: 1133 601 BlpI GC'TnA_GC Cuts at: 0 157 655 1734 Size: 157 498 1079 Fragments arranged by size: 1079 498 157 Bme1580I G_kGCm'C Cuts at: 0 668 683 777 1009 1734 Size: 668 15 94 232 725 Fragments arranged by size: 725 668 232 94 15 BmgBI CAC'GTC Cuts at: 0 234 1164 1734 Size: 234 930 570 Fragments arranged by size: 930 570 234 BmrI ACTGGGnnnn_n' Cuts at: 0 1540 1734 Size: 1540 194 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 225 1734 Size: 225 1509 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 772 820 856 1269 1734 Size: 772 48 36 413 465 Fragments arranged by size: 772 465 413 48 36 BsaAI yAC'GTr Cuts at: 0 499 1452 1548 1590 1734 Size: 499 953 96 42 144 Fragments arranged by size: 953 499 144 96 42 BsaHI Gr'CG_yC Cuts at: 0 101 674 1081 1207 1640 1734 Size: 101 573 407 126 433 94 Fragments arranged by size: 573 433 407 126 101 94 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 770 800 1657 1687 1734 Size: 770 30 857 30 47 Fragments arranged by size: 857 770 47 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 817 853 856 859 871 1734 Size: 817 36 3 3 12 863 Fragments arranged by size: 863 817 36 12 3 3 BseYI C'CCAG_C Cuts at: 0 441 605 1706 1734 Size: 441 164 1101 28 Fragments arranged by size: 1101 441 164 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 322 373 715 1385 1390 1507 1734 Size: 322 51 342 670 5 117 227 Fragments arranged by size: 670 342 322 227 117 51 5 BsiEI CG_ry'CG Cuts at: 0 123 369 1560 1734 Size: 123 246 1191 174 Fragments arranged by size: 1191 246 174 123 BsiHKAI G_wGCw'C Cuts at: 0 668 1418 1733 1734 Size: 668 750 315 1 Fragments arranged by size: 750 668 315 1 Bsp1286I G_dGCh'C Cuts at: 0 242 668 683 719 777 863 1009 1285 Size: 242 426 15 36 58 86 146 276 Cuts at: 1285 1418 1733 1734 Size: 133 315 1 Fragments arranged by size: 426 315 276 242 146 133 86 58 36 15 1 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 41 383 555 566 650 841 1734 Size: 41 342 172 11 84 191 893 Fragments arranged by size: 893 342 191 172 84 41 11 BsrBI CCG'CTC Cuts at: 0 531 1734 Size: 531 1203 BsrFI r'CCGG_y Cuts at: 0 281 550 1252 1265 1734 Size: 281 269 702 13 469 Fragments arranged by size: 702 469 281 269 13 BsrGI T'GTAC_A Cuts at: 0 272 1734 Size: 272 1462 BssHII G'CGCG_C Cuts at: 0 214 331 1517 1734 Size: 214 117 1186 217 Fragments arranged by size: 1186 217 214 117 BstAPI GCAn_nnn'nTGC Cuts at: 0 75 438 702 1485 1503 1734 Size: 75 363 264 783 18 231 Fragments arranged by size: 783 363 264 231 75 18 BstXI CCAn_nnnn'nTGG Cuts at: 0 405 1734 Size: 405 1329 BstYI r'GATC_y Cuts at: 0 1133 1734 Size: 1133 601 BtgI C'CryG_G Cuts at: 0 245 1076 1635 1665 1734 Size: 245 831 559 30 69 Fragments arranged by size: 831 559 245 69 30 BtsI GCAGTG_nn' Cuts at: 0 106 1324 1402 1734 Size: 106 1218 78 332 Fragments arranged by size: 1218 332 106 78 DraIII CAC_nnn'GTG Cuts at: 0 1534 1734 Size: 1534 200 EaeI y'GGCC_r Cuts at: 0 95 120 366 548 1557 1734 Size: 95 25 246 182 1009 177 Fragments arranged by size: 1009 246 182 177 95 25 EagI C'GGCC_G Cuts at: 0 120 366 1557 1734 Size: 120 246 1191 177 Fragments arranged by size: 1191 246 177 120 EcoICRI GAG'CTC Cuts at: 0 1416 1734 Size: 1416 318 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 772 820 856 1075 1111 1269 1734 Size: 772 48 36 219 36 158 465 Fragments arranged by size: 772 465 219 158 48 36 36 EcoNI CCTnn'n_nnAGG Cuts at: 0 562 724 757 1057 1734 Size: 562 162 33 300 677 Fragments arranged by size: 677 562 300 162 33 EcoO109I rG'GnC_Cy Cuts at: 0 594 686 807 1670 1734 Size: 594 92 121 863 64 Fragments arranged by size: 863 594 121 92 64 HaeII r_GCGC'y Cuts at: 0 75 157 316 835 1084 1292 1643 1734 Size: 75 82 159 519 249 208 351 91 Fragments arranged by size: 519 351 249 208 159 91 82 75 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1009 1041 1734 Size: 1009 32 693 Fragments arranged by size: 1009 693 32 HincII GTy'rAC Cuts at: 0 1028 1734 Size: 1028 706 KasI G'GCGC_C Cuts at: 0 1080 1639 1734 Size: 1080 559 95 Fragments arranged by size: 1080 559 95 MspA1I CmG'CkG Cuts at: 0 1202 1562 1734 Size: 1202 360 172 Fragments arranged by size: 1202 360 172 NaeI GCC'GGC Cuts at: 0 283 552 1254 1267 1734 Size: 283 269 702 13 467 Fragments arranged by size: 702 467 283 269 13 NarI GG'CG_CC Cuts at: 0 1081 1640 1734 Size: 1081 559 94 Fragments arranged by size: 1081 559 94 NgoMIV G'CCGG_C Cuts at: 0 281 550 1252 1265 1734 Size: 281 269 702 13 469 Fragments arranged by size: 702 469 281 269 13 NotI GC'GGCC_GC Cuts at: 0 366 1557 1734 Size: 366 1191 177 Fragments arranged by size: 1191 366 177 NspI r_CATG'y Cuts at: 0 46 57 743 1734 Size: 46 11 686 991 Fragments arranged by size: 991 686 46 11 PasI CC'CwG_GG Cuts at: 0 759 1734 Size: 759 975 PflMI CCAn_nnn'nTGG Cuts at: 0 461 911 1475 1734 Size: 461 450 564 259 Fragments arranged by size: 564 461 450 259 PfoI T'CCnGG_A Cuts at: 0 725 1066 1685 1734 Size: 725 341 619 49 Fragments arranged by size: 725 619 341 49 PmlI CAC'GTG Cuts at: 0 499 1590 1734 Size: 499 1091 144 Fragments arranged by size: 1091 499 144 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 493 525 1734 Size: 493 32 1209 Fragments arranged by size: 1209 493 32 PpuMI rG'GwC_Cy Cuts at: 0 807 1734 Size: 807 927 PshAI GACnn'nnGTC Cuts at: 0 1210 1734 Size: 1210 524 PspXI vC'TCGA_Gb Cuts at: 0 589 1734 Size: 589 1145 PstI C_TGCA'G Cuts at: 0 71 380 518 566 1319 1734 Size: 71 309 138 48 753 415 Fragments arranged by size: 753 415 309 138 71 48 PvuII CAG'CTG Cuts at: 0 1202 1734 Size: 1202 532 SacI G_AGCT'C Cuts at: 0 1418 1734 Size: 1418 316 SexAI A'CCwGG_T Cuts at: 0 1021 1274 1734 Size: 1021 253 460 Fragments arranged by size: 1021 460 253 SfcI C'TryA_G Cuts at: 0 67 376 514 562 1315 1734 Size: 67 309 138 48 753 419 Fragments arranged by size: 753 419 309 138 67 48 SfoI GGC'GCC Cuts at: 0 1082 1641 1734 Size: 1082 559 93 Fragments arranged by size: 1082 559 93 SmaI CCC'GGG Cuts at: 0 631 1464 1734 Size: 631 833 270 Fragments arranged by size: 833 631 270 SmlI C'TyrA_G Cuts at: 0 589 1734 Size: 589 1145 SphI G_CATG'C Cuts at: 0 46 743 1734 Size: 46 697 991 Fragments arranged by size: 991 697 46 SrfI GCCC'GGGC Cuts at: 0 631 1734 Size: 631 1103 StuI AGG'CCT Cuts at: 0 460 1039 1063 1603 1734 Size: 460 579 24 540 131 Fragments arranged by size: 579 540 460 131 24 StyI C'CwwG_G Cuts at: 0 455 873 1734 Size: 455 418 861 Fragments arranged by size: 861 455 418 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 597 1048 1522 1734 Size: 597 451 474 212 Fragments arranged by size: 597 474 451 212 TatI w'GTAC_w Cuts at: 0 272 1298 1734 Size: 272 1026 436 Fragments arranged by size: 1026 436 272 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 245 277 770 802 1657 1689 1734 Size: 245 32 493 32 855 32 45 Fragments arranged by size: 855 493 245 45 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 766 912 1161 1683 1734 Size: 766 146 249 522 51 Fragments arranged by size: 766 522 249 146 51 XhoI C'TCGA_G Cuts at: 0 589 1734 Size: 589 1145 XmaI C'CCGG_G Cuts at: 0 629 1462 1734 Size: 629 833 272 Fragments arranged by size: 833 629 272 ZraI GAC'GTC Cuts at: 0 102 1734 Size: 102 1632 Enzymes that do cut: AarI AatII AccI AcuI AflIII AhdI AlfI AlwNI ApaLI AvaI BanI BanII BbeI BbsI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstXI BstYI BtgI BtsI DraIII EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MspA1I NaeI NarI NgoMIV NotI NspI PasI PflMI PfoI PmlI PpiI PpuMI PshAI PspXI PstI PvuII SacI SexAI SfcI SfoI SmaI SmlI SphI SrfI StuI StyI TaqII TatI TstI XcmI XhoI XmaI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AgeI AleI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BmtI Bpu10I BpuEI BsaI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrDI BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EarI EciI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI MscI MslI NcoI NdeI NheI NruI NsiI PacI PciI PmeI PsiI PspOMI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SspI SwaI Tth111I XbaI XmnI