RNFT1 ORF clone pFN21AE4083

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Halotag ORF Clone pFN21AE4083

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Clone info.

Species

human

Clone Name

pFN21AE4083 Sequence (with vector) Restriction Map

Product ID

FHC26132

ORF Sequence

DNA sequence (1305 bp)
Protein sequence (435 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4083

Symbol

RNFT1

Alias : PTD016

Description

ring finger protein, transmembrane 1

Original Clone ID

cp94083

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1305 bp
Restriction map A Restriction map B

Features of the protein sequence

Description

Length: 435 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11622.2 6.5e-130 100.0 RNFT1 CCDS44987.1 6.4e-44 39.8 RNFT2 CCDS9180.2 6.6e-33 36.8 RNFT2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR018957 375 412 PF00097 Zinc finger HMMSmart IPR001841 375 412 SM00184 Zinc finger ProfileScan IPR001841 375 413 PS50089 Zinc finger
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 147 LFKWLQKSLPYILILSVKLVMQH 169 SECONDARY 23 2 173 ISLGIGLLTTFMYANKSIVNQVF 195 SECONDARY 23 3 203 IQCAWLLVFLAGSSVLLYYTFHS 225 PRIMARY 23 4 254 TDFILKFFFMGLKCLILLVPSFI 276 PRIMARY 23 5 292 LCQYYRTFVPIPVWFRYLISYGE 314 SECONDARY 23 6 320 RWSLGILLALLYLILKLLEFFGH 342 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_057209 435 615172 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4083 Download

>KIEE4083  1305 bp
ATGCCGCTGTTCTTGCTGTCGCTCCCGACACCTCCGTCCGCTTCTGGTCATGAGAGGAGA
CAGAGGCCTGAAGCAAAGACATCTGGGTCAGAGAAAAAGTATTTAAGGGCCATGCAAGCC
AATCGTAGCCAACTGCACAGTCCTCCAGGAACTGGAAGCAGTGAGGATGCCTCAACCCCT
CAGTGTGTCCACACAAGATTGACAGGAGAGGGTTCTTGCCCTCATTCTGGAGATGTTCAT
ATCCAGATAAACTCCATACCTAAAGAATGTGCAGAAAATGCAAGCTCCAGAAATATAAGG
TCAGGTGTCCATAGCTGTGCCCATGGATGTGTACACAGTCGCTTACGGGGTCACTCCCAC
AGTGAAGCAAGGCTGACTGATGATACTGCCGCAGAATCTGGAGATCATGGTAGTAGCTCC
TTCTCAGAATTCCGCTATCTCTTCAAGTGGCTGCAAAAAAGTCTTCCATATATTTTGATT
CTGAGCGTCAAACTTGTTATGCAGCATATAACAGGAATTTCTCTTGGAATTGGGCTGCTA
ACAACTTTTATGTATGCAAACAAAAGCATTGTAAATCAGGTTTTTCTAAGAGAAAGGTCC
TCAAAGATTCAGTGTGCTTGGTTACTGGTATTCTTAGCAGGATCTTCTGTTCTTTTATAT
TACACCTTTCATTCTCAGTCACTTTATTACAGCTTAATTTTTTTAAATCCTACTTTGGAC
CATTTGAGCTTCTGGGAAGTATTTTGGATTGTTGGAATTACAGACTTCATTCTGAAATTC
TTTTTCATGGGCTTAAAATGCCTTATTTTATTGGTGCCTTCTTTCATCATGCCTTTTAAA
TCTAAGGGTTACTGGTATATGCTTTTAGAAGAATTGTGTCAATACTACCGAACTTTTGTT
CCCATACCAGTTTGGTTTCGCTACCTTATAAGCTATGGGGAGTTTGGTAACGTAACTAGA
TGGAGTCTTGGGATACTGCTGGCTTTACTCTACCTCATATTAAAACTTTTGGAATTTTTT
GGGCATCTGAGAACTTTCAGACAGGTTTTACGAATATTTTTTACACAACCAAGTTATGGA
GTGGCTGCCAGCAAGAGACAGTGTTCAGATGTGGATGATATTTGTTCAATATGTCAAGCT
GAATTTCAGAAGCCAATTCTTCTCATCTGTCAGCATATATTTTGTGAAGAGTGCATGACC
TTATGGTTTAACAGAGAGAAAACATGTCCACTCTGCAGAACTGTGATTTCAGACCATATA
AACAAATGGAAGGATGGAGCCACTTCATCACACCTTCAAATATAT

ORF Protein

Cloned ORF protein sequence for pFN21AE4083 Download

>KIEE4083  435 aa
MPLFLLSLPTPPSASGHERRQRPEAKTSGSEKKYLRAMQANRSQLHSPPGTGSSEDASTP
QCVHTRLTGEGSCPHSGDVHIQINSIPKECAENASSRNIRSGVHSCAHGCVHSRLRGHSH
SEARLTDDTAAESGDHGSSSFSEFRYLFKWLQKSLPYILILSVKLVMQHITGISLGIGLL
TTFMYANKSIVNQVFLRERSSKIQCAWLLVFLAGSSVLLYYTFHSQSLYYSLIFLNPTLD
HLSFWEVFWIVGITDFILKFFFMGLKCLILLVPSFIMPFKSKGYWYMLLEELCQYYRTFV
PIPVWFRYLISYGEFGNVTRWSLGILLALLYLILKLLEFFGHLRTFRQVLRIFFTQPSYG
VAASKRQCSDVDDICSICQAEFQKPILLICQHIFCEECMTLWFNREKTCPLCRTVISDHI
NKWKDGATSSHLQIY

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4083 Download

>pFN21AE4083  6010 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCGCTGTTCTTGCTGTCGCTCCCGACACCTCC
GTCCGCTTCTGGTCATGAGAGGAGACAGAGGCCTGAAGCAAAGACATCTGGGTCAGAGAA
AAAGTATTTAAGGGCCATGCAAGCCAATCGTAGCCAACTGCACAGTCCTCCAGGAACTGG
AAGCAGTGAGGATGCCTCAACCCCTCAGTGTGTCCACACAAGATTGACAGGAGAGGGTTC
TTGCCCTCATTCTGGAGATGTTCATATCCAGATAAACTCCATACCTAAAGAATGTGCAGA
AAATGCAAGCTCCAGAAATATAAGGTCAGGTGTCCATAGCTGTGCCCATGGATGTGTACA
CAGTCGCTTACGGGGTCACTCCCACAGTGAAGCAAGGCTGACTGATGATACTGCCGCAGA
ATCTGGAGATCATGGTAGTAGCTCCTTCTCAGAATTCCGCTATCTCTTCAAGTGGCTGCA
AAAAAGTCTTCCATATATTTTGATTCTGAGCGTCAAACTTGTTATGCAGCATATAACAGG
AATTTCTCTTGGAATTGGGCTGCTAACAACTTTTATGTATGCAAACAAAAGCATTGTAAA
TCAGGTTTTTCTAAGAGAAAGGTCCTCAAAGATTCAGTGTGCTTGGTTACTGGTATTCTT
AGCAGGATCTTCTGTTCTTTTATATTACACCTTTCATTCTCAGTCACTTTATTACAGCTT
AATTTTTTTAAATCCTACTTTGGACCATTTGAGCTTCTGGGAAGTATTTTGGATTGTTGG
AATTACAGACTTCATTCTGAAATTCTTTTTCATGGGCTTAAAATGCCTTATTTTATTGGT
GCCTTCTTTCATCATGCCTTTTAAATCTAAGGGTTACTGGTATATGCTTTTAGAAGAATT
GTGTCAATACTACCGAACTTTTGTTCCCATACCAGTTTGGTTTCGCTACCTTATAAGCTA
TGGGGAGTTTGGTAACGTAACTAGATGGAGTCTTGGGATACTGCTGGCTTTACTCTACCT
CATATTAAAACTTTTGGAATTTTTTGGGCATCTGAGAACTTTCAGACAGGTTTTACGAAT
ATTTTTTACACAACCAAGTTATGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGATGTGGA
TGATATTTGTTCAATATGTCAAGCTGAATTTCAGAAGCCAATTCTTCTCATCTGTCAGCA
TATATTTTGTGAAGAGTGCATGACCTTATGGTTTAACAGAGAGAAAACATGTCCACTCTG
CAGAACTGTGATTTCAGACCATATAAACAAATGGAAGGATGGAGCCACTTCATCACACCT
TCAAATATATGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT