(Circular) (Six-base) MAPSORT of: pFN21AE4083.seq Check: 591 from: 1 to: 6010 pFN21AE4083 6010 bp With 182 enzymes: SgfI * March 28, 2012 13:10 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3820 278 Size: 53 83 186 936 2284 2468 Fragments arranged by size: 2468 2284 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3353 1937 Size: 1416 4594 Acc65I G'GTAC_C Cuts at: 3331 3331 Size: 6010 AclI AA'CG_TT Cuts at: 1563 4137 4510 1563 Size: 2574 373 3063 Fragments arranged by size: 3063 2574 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2094 4073 5457 5725 1632 Size: 462 1979 1384 268 1917 Fragments arranged by size: 1979 1917 1384 462 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5813 AflIII A'CryG_T Cuts at: 1203 3227 4909 5886 1203 Size: 2024 1682 977 1327 Fragments arranged by size: 2024 1682 1327 977 AgeI A'CCGG_T Cuts at: 4841 4841 Size: 6010 AhdI GACnn_n'nnGTC Cuts at: 4739 4739 Size: 6010 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6010 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6010 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2637 2669 2887 2919 2637 Size: 32 218 32 5728 Fragments arranged by size: 5728 218 32 32 AlwNI CAG_nnn'CTG Cuts at: 2156 2402 3753 5325 2156 Size: 246 1351 1572 2841 Fragments arranged by size: 2841 1572 1351 246 ApaLI G'TGCA_C Cuts at: 4067 5223 4067 Size: 1156 4854 ApoI r'AATT_y Cuts at: 1493 2432 2520 2780 3017 3146 3319 3545 3697 Size: 939 88 260 237 129 173 226 152 Cuts at: 3697 5806 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 939 260 237 226 173 152 129 88 AseI AT'TA_AT Cuts at: 160 1032 4564 160 Size: 872 3532 1606 Fragments arranged by size: 3532 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 AvaI C'yCGr_G Cuts at: 1870 1943 3335 1870 Size: 73 1392 4545 Fragments arranged by size: 4545 1392 73 BamHI G'GATC_C Cuts at: 3340 3707 3340 Size: 367 5643 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2818 3331 4786 618 Size: 325 217 627 1031 513 1455 1842 Fragments arranged by size: 1842 1455 1031 627 513 325 217 BanII G_rGCy'C Cuts at: 1391 3329 1391 Size: 1938 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2459 928 Size: 971 560 4479 Fragments arranged by size: 4479 971 560 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2108 2142 4201 4235 1903 Size: 34 50 34 87 34 2059 34 3678 Fragments arranged by size: 3678 2059 87 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2970 3902 5112 2970 Size: 932 1210 3868 Fragments arranged by size: 3868 1210 932 BclI T'GATC_A Cuts at: 1548 1548 Size: 6010 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4621 136 Size: 107 122 71 631 605 2949 1525 Fragments arranged by size: 2949 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6005 6005 Size: 6010 BlpI GC'TnA_GC Cuts at: 3427 3427 Size: 6010 Bme1580I G_kGCm'C Cuts at: 1790 2326 4071 5227 1790 Size: 536 1745 1156 2573 Fragments arranged by size: 2573 1745 1156 536 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6010 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3741 4699 442 Size: 416 53 83 399 24 2324 958 1753 Fragments arranged by size: 2324 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6010 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2191 2191 Size: 6010 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2133 2253 2275 2424 4670 1239 Size: 894 120 22 149 2246 2579 Fragments arranged by size: 2579 2246 894 149 120 22 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4135 5000 5298 4135 Size: 865 298 4847 Fragments arranged by size: 4847 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4673 882 Size: 617 3174 2219 Fragments arranged by size: 3174 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3801 493 Size: 711 2597 2702 Fragments arranged by size: 2702 2597 711 BsaBI GATnn'nnATC Cuts at: 6004 6004 Size: 6010 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3817 4199 4834 275 Size: 53 83 186 936 2284 382 635 1451 Fragments arranged by size: 2284 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4443 4841 5115 5262 5673 4443 Size: 398 274 147 411 4780 Fragments arranged by size: 4780 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5785 Fragments arranged by size: 5785 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2074 1186 Size: 888 5122 BseYI C'CCAG_C Cuts at: 5213 5709 5213 Size: 496 5514 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2123 2295 1163 Size: 573 387 172 4878 Fragments arranged by size: 4878 573 387 172 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3460 4221 5249 1540 Size: 275 186 1459 761 1028 2301 Fragments arranged by size: 2301 1459 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4071 4156 5227 4071 Size: 85 1071 4854 Fragments arranged by size: 4854 1071 85 BsmI GAATG_Cn' Cuts at: 3521 3614 3521 Size: 93 5917 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2326 3329 4071 4156 5227 1391 Size: 399 536 1003 742 85 1071 2174 Fragments arranged by size: 2174 1071 1003 742 536 399 85 BspEI T'CCGG_A Cuts at: 5673 5673 Size: 6010 BspHI T'CATG_A Cuts at: 2053 3899 5629 2053 Size: 1846 1730 2434 Fragments arranged by size: 2434 1846 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3365 844 Size: 2521 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3897 1394 Size: 429 2074 3507 Fragments arranged by size: 3507 2074 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4505 4679 65 Size: 1183 3257 174 1396 Fragments arranged by size: 3257 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4654 4841 1700 Size: 110 2844 187 2869 Fragments arranged by size: 2869 2844 187 110 BsrGI T'GTAC_A Cuts at: 96 2335 96 Size: 2239 3771 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6010 BssSI C'ACGA_G Cuts at: 4070 5082 4070 Size: 1012 4998 BstAPI GCAn_nnn'nTGC Cuts at: 794 2282 794 Size: 1488 4522 BstBI TT'CG_AA Cuts at: 4814 4814 Size: 6010 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6010 BstYI r'GATC_y Cuts at: 1972 2645 3340 3707 4092 4109 5561 5647 5659 Size: 673 695 367 385 17 1452 86 12 Cuts at: 5659 6005 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 695 673 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2326 513 Size: 552 1261 4197 Fragments arranged by size: 4197 1261 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5271 Fragments arranged by size: 5271 683 56 BtsI GCAGTG_nn' Cuts at: 2170 3526 4320 4340 2170 Size: 1356 794 20 3840 Fragments arranged by size: 3840 1356 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6010 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5975 DraI TTT'AAA Cuts at: 2709 2842 3314 2709 Size: 133 472 5405 Fragments arranged by size: 5405 472 133 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2038 2089 5017 809 Size: 507 722 51 2928 1802 Fragments arranged by size: 2928 1802 722 507 51 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3457 4346 5975 Size: 54 1425 50 275 18 1627 889 1629 Cuts at: 5975 8 Size: 43 Fragments arranged by size: 1629 1627 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3457 1537 Size: 275 1645 4090 Fragments arranged by size: 4090 1645 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2450 3186 3940 1353 Size: 1097 736 754 3423 Fragments arranged by size: 3423 1097 754 736 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4597 4971 5117 387 Size: 1064 138 3008 374 146 1280 Fragments arranged by size: 3008 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2133 2253 2275 2424 4073 4670 Size: 393 462 39 120 22 149 1649 597 Cuts at: 4670 5457 5725 1239 Size: 787 268 1524 Fragments arranged by size: 1649 1524 787 597 462 393 268 149 120 39 22 EcoO109I rG'GnC_Cy Cuts at: 2601 2601 Size: 6010 EcoRI G'AATT_C Cuts at: 2432 3319 3697 2432 Size: 887 378 4745 Fragments arranged by size: 4745 887 378 FspI TGC'GCA Cuts at: 4516 4516 Size: 6010 HaeII r_GCGC'y Cuts at: 1678 5157 1678 Size: 3479 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4651 4683 4725 4757 1373 Size: 32 135 32 3079 32 42 32 2626 Fragments arranged by size: 3079 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3354 3600 669 Size: 988 281 1416 246 3079 Fragments arranged by size: 3079 1416 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6010 HpaI GTT'AAC Cuts at: 3600 3600 Size: 6010 KpnI G_GTAC'C Cuts at: 3335 3335 Size: 6010 MfeI C'AATT_G Cuts at: 3609 3609 Size: 6010 MluI A'CGCG_T Cuts at: 5886 5886 Size: 6010 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2737 5124 5308 1305 Size: 1432 2387 184 2007 Fragments arranged by size: 2387 2007 1432 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4188 Fragments arranged by size: 4188 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2319 2331 3968 4327 4486 518 Size: 648 1153 12 1637 359 159 2042 Fragments arranged by size: 2042 1637 1153 648 359 159 12 MspA1I CmG'CkG Cuts at: 1606 2011 3426 4103 5251 5496 1606 Size: 405 1415 677 1148 245 2120 Fragments arranged by size: 2120 1415 1148 677 405 245 NcoI C'CATG_G Cuts at: 513 1065 2326 513 Size: 552 1261 4197 Fragments arranged by size: 4197 1261 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6010 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6010 NotI GC'GGCC_GC Cuts at: 3457 3457 Size: 6010 NspI r_CATG'y Cuts at: 1132 1229 3231 3368 4913 1132 Size: 97 2002 137 1545 2229 Fragments arranged by size: 2229 2002 1545 137 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6010 PciI A'CATG_T Cuts at: 3227 4909 3227 Size: 1682 4328 PflMI CCAn_nnn'nTGG Cuts at: 1108 2156 3080 1108 Size: 1048 924 4038 Fragments arranged by size: 4038 1048 924 PfoI T'CCnGG_A Cuts at: 2149 2149 Size: 6010 PmeI GTTT'AAAC Cuts at: 3314 3314 Size: 6010 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3091 3123 4079 4111 5592 5624 3091 Size: 32 956 32 1481 32 3477 Fragments arranged by size: 3477 1481 956 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2601 2601 Size: 6010 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6010 PsiI TTA'TAA Cuts at: 2933 3580 2933 Size: 647 5363 PstI C_TGCA'G Cuts at: 830 3242 3362 830 Size: 2412 120 3478 Fragments arranged by size: 3478 2412 120 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6010 SalI G'TCGA_C Cuts at: 1936 3352 1936 Size: 1416 4594 SbfI CC_TGCA'GG Cuts at: 3362 3362 Size: 6010 ScaI AGT'ACT Cuts at: 4258 5970 4258 Size: 1712 4298 SfcI C'TryA_G Cuts at: 826 1045 3238 3358 4493 5174 5365 826 Size: 219 2193 120 1135 681 191 1471 Fragments arranged by size: 2193 1471 1135 681 219 191 120 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6010 SmaI CCC'GGG Cuts at: 1872 3337 1872 Size: 1465 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4114 5015 5277 820 Size: 197 926 2171 901 262 1553 Fragments arranged by size: 2171 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3801 493 Size: 3308 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6010 SphI G_CATG'C Cuts at: 1132 3368 1132 Size: 2236 3774 SspI AAT'ATT Cuts at: 5 52 3060 3934 5 Size: 47 3008 874 2081 Fragments arranged by size: 3008 2081 874 47 StuI AGG'CCT Cuts at: 1517 2071 1517 Size: 554 5456 StyI C'CwwG_G Cuts at: 513 1065 2326 3449 513 Size: 552 1261 1123 3074 Fragments arranged by size: 3074 1261 1123 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4037 4054 4207 4392 4837 325 Size: 1255 2457 17 153 185 445 1498 Fragments arranged by size: 2457 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2335 4256 5968 Size: 274 80 33 51 1443 358 1921 1712 Cuts at: 5968 96 Size: 138 Fragments arranged by size: 1921 1712 1443 358 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5970 6002 5970 Size: 32 5978 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6010 XbaI T'CTAG_A Cuts at: 3346 3346 Size: 6010 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6010 XmaI C'CCGG_G Cuts at: 1870 3335 1870 Size: 1465 4545 XmnI GAAnn'nnTTC Cuts at: 4139 4139 Size: 6010 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3818 276 Size: 53 83 186 936 2284 2468 Fragments arranged by size: 2468 2284 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI