Restriction map for
pFN21AE4083
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# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbvCI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI BspDI BspEI
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
HphI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SwaI TaqI
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AfaI 331 GTAC GT^AC
AflIII 1222 ACATGT A^C[GA][CT]GT
AlwI 640 GGATC GGATC(4/5)
BanI 813 GGTGCC G^G[CT][GA]CC
BbsI 461 GTCTTC GAAGAC(2/6)
BcgI 114 GCAAGCCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 971 GGATAC GTATCC(6/5)
BfaI 956 CTAG C^TAG
BmyI 317 GTGCCC G[AGT]GC[ACT]^C
BpuAI 461 GTCTTC GAAGAC(2/6)
BsaJI 321 CCATGG C^C[ACGT][ACGT]GG
BseRI 54 GAGGAG GAGGAG(10/8)
Bsp1286I 317 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 330 TGTACA T^GTACA
BspHI 48 TCATGA T^CATGA
BspLU11I 1222 ACATGT A^CATGT
BsrGI 330 TGTACA T^GTACA
BssKI 145 CCAGG ^CC[ACGT]GG
BstAPI 271 GCAGAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 145 CCAGG CC^[AT]GG
BstYI 640 GGATCT [GA]^GATC[CT]
DraII 595 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 321 CCATGG C^C[GA][CT]GG
EcoO109I 595 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 427 GAATTC G^AATTC
EcoRII 145 CCAGG ^CC[AT]GG
EcoT14I 321 CCATGG C^C[AT][AT]GG
HgaI 485 GCGTC GACGC(5/10)
MaeI 956 CTAG C^TAG
MaeII 950 ACGT A^CGT
MflI 640 GGATCT [GA]^GATC[CT]
MspA1I 4 CCGCTG C[AC]G^C[GT]G
MvaI 145 CCAGG CC^[AT]GG
NcoI 321 CCATGG C^CATGG
NspI 1222 ACATGT [GA]CATG^[CT]
PleI 963 GAGTC GAGTC(4/5)
PpuMI 595 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 1233 CTGCAG CTGCA^G
RcaI 48 TCATGA T^CATGA
RsaI 331 GTAC GT^AC
ScrFI 145 CCAGG CC^[ACGT]GG
SfcI 1233 CTGCAG C^T[GA][CT]AG
SspBI 330 TGTACA T^GTACA
SspI 1053 AATATT AAT^ATT
StuI 64 AGGCCT AGG^CCT
StyI 321 CCATGG C^C[AT][AT]GG
XhoII 640 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 146 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
392 CAGAATCTG
AvaII 596 GGTCC G^G[AT]CC
717 GGACC
BsgI 133 CTGCAC GTGCAG(16/14)
269 GTGCAG
BsmAI 57 GAGAC GTCTC(1/5)
1095 GAGAC
DpnII 403 GATC ^GATC
641 GATC
DraI 702 TTTAAA TTT^AAA
835 TTTAAA
DrdI 27 GACACCTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
78 GACATCTGGGTC
EarI 439 CTCTTC CTCTTC(1/4)
1186 GAAGAG
HaeIII 65 GGCC GG^CC
108 GGCC
Ksp632I 439 CTCTTC CTCTTC(1/4)
1186 GAAGAG
MboI 403 GATC ^GATC
641 GATC
MslI 310 CATAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
322 CATGGATGTG
NdeII 403 GATC ^GATC
641 GATC
NlaIV 813 GGTGCC GG[ACGT]^[ACGT]CC
1276 GGAGCC
PflMI 145 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1069 CCAAGTTATGG
Sau3AI 403 GATC ^GATC
641 GATC
SfaNI 166 GATGC GCATC(5/9)
1023 GCATC
Tsp45I 350 GTCAC ^GT[GC]AC
678 GTCAC
Van91I 145 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1069 CCAAGTTATGG
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BpmI BsiYI BslI BsrI BstF5I
Cac8I Cfr13I DdeI Fnu4HI FokI
HinfI ItaI MaeIII MboII MnlI
MseI MwoI NlaIII Sau96I TfiI
Tru9I TseI Tsp509I TspRI