ZNRF4 ORF clone pFN21AE4082

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE4082

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Clone info.

Species

human

Clone Name

pFN21AE4082 Sequence (with vector) Restriction Map

Product ID

FHC29888

ORF Sequence

DNA sequence (1287 bp)
Protein sequence (429 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4082

Symbol

ZNRF4

Alias : RNF204, SPERIZIN, Ssrzf1, spzn

Description

zinc and ring finger 4

Original Clone ID

cp94082

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1287 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 429 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS42475.1 3.5e-126 99.5 ZNRF4 CCDS3146.1 1.5e-27 40.8 RNF13 CCDS11060.1 8.7e-27 39.7 RNF167 CCDS82040.1 1.9e-25 40.1 RNF167 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003137 156 222 PF02225 Protease-associated domain IPR018957 309 351 PF00097 Zinc finger HMMSmart IPR001841 309 351 SM00184 Zinc finger ProfileScan IPR001841 309 352 PS50089 Zinc finger
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 80 LVAVKALLVLSLLQVPAQAVVRA 102 SECONDARY 23 2 253 TVSWVLGCTLALVVSAFFVLNHL 275 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_859061 429 612063 99.5 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4082 Download

>KIEE4082  1287 bp
ATGCCGCTCTGCCGTCCGGAGCACTTAATGCCTAGAGCCAGCAGGGTCCCAGTGGCCGCG
TCACTGCCTCTGAGCCACGCGGTCATTCCAACTCAACTGCCCTCGCGTCCTGGCCACAGG
CCCCCTGGGAGACCCCGGAGATGCCCAAAGGCCTCATGCCTGCCGCCTCCAGTGGGACCT
AGCAGCACACAGACAGCGAAGCGGGTGACCATGGGGTGGCCACGGCCGGGCCGAGCCCTC
GTGGCAGTCAAAGCCTTGCTGGTCTTGTCGCTGCTCCAGGTGCCCGCGCAGGCAGTGGTA
CGGGCCGTGCTGGAAGACAACTCGAGCTCGGTGGACTTTGCGGATCTGCCGGCGCTGTTC
GGCGTCCCCCTGGCCCCCGAGGGCATACGGGGCTACCTGATGGAGGTCAAGCCAGCCAAC
GCGTGCCATCCCATCGAGGCCCCGCGACTGGGCAACCGCTCTCTGGGCTCCATTGCGCTG
ATCCGCCGCTACGACTGCACCTTCGACCTCAAGGTGCTGAACGCCCAGCGCGCCGGCTTC
GAGGCGGCCATCGTGCACAACGTCCACTCCGACGACCTCGTGAGCATGACCCACGTCTAC
GAGGACTTGAGGGGCCAGATCGCCATCCCCTCAGTGTTCGTGAGCGAGGCCGCCTCGCAG
GACCTGCGGGTCATCCTGGGCTGCAACAAGTCGGCCCACGCGCTGCTCCTGCCCGACGAC
CCACCGTGCCACGACCTGGGCTGTCACCCCGTGCTGACCGTGTCCTGGGTGCTGGGCTGT
ACCCTGGCCCTGGTCGTATCAGCCTTCTTTGTCCTGAACCACCTGTGGCTCTGGGCCCAG
GCCTGCTGCAGCCACAGACGGCCGGTGAAGACGTCTACCTGCCAGAAGGCCCAGGTCCGC
ACCTTCACGTGGCACAACGACCTGTGTGCCATCTGCCTGGATGAGTACGAGGAGGGCGAC
CAACTCAAGATCCTGCCCTGCTCCCACACCTACCACTGCAAATGCATTGACCCCTGGTTC
TCCCAAGCCCCCCGGCGCTCCTGCCCCGTGTGCAAACAGTCGGTGGCCGCCACAGAAGAC
AGCTTTGACTCCACCACCTACAGCTTCAGGGACGAGGACCCCTCCCTACCGGGCCACCGG
CCCCCCATCTGGGCCATTCAAGTCCAGCTACGCTCCCGGAGGCTGGAGCTGCTGGGCCGC
GCCAGTCCCCACTGCCACTGCAGCACCACGTCCCTGGAGGCAGAGTATACCACTGTCTCC
TCAGCCCCTCCTGAGGCCCCTGGTCAG

ORF Protein

Cloned ORF protein sequence for pFN21AE4082 Download

>KIEE4082  429 aa
MPLCRPEHLMPRASRVPVAASLPLSHAVIPTQLPSRPGHRPPGRPRRCPKASCLPPPVGP
SSTQTAKRVTMGWPRPGRALVAVKALLVLSLLQVPAQAVVRAVLEDNSSSVDFADLPALF
GVPLAPEGIRGYLMEVKPANACHPIEAPRLGNRSLGSIALIRRYDCTFDLKVLNAQRAGF
EAAIVHNVHSDDLVSMTHVYEDLRGQIAIPSVFVSEAASQDLRVILGCNKSAHALLLPDD
PPCHDLGCHPVLTVSWVLGCTLALVVSAFFVLNHLWLWAQACCSHRRPVKTSTCQKAQVR
TFTWHNDLCAICLDEYEEGDQLKILPCSHTYHCKCIDPWFSQAPRRSCPVCKQSVAATED
SFDSTTYSFRDEDPSLPGHRPPIWAIQVQLRSRRLELLGRASPHCHCSTTSLEAEYTTVS
SAPPEAPGQ

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4082 Download

>pFN21AE4082  5992 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCGCTCTGCCGTCCGGAGCACTTAATGCCTAG
AGCCAGCAGGGTCCCAGTGGCCGCGTCACTGCCTCTGAGCCACGCGGTCATTCCAACTCA
ACTGCCCTCGCGTCCTGGCCACAGGCCCCCTGGGAGACCCCGGAGATGCCCAAAGGCCTC
ATGCCTGCCGCCTCCAGTGGGACCTAGCAGCACACAGACAGCGAAGCGGGTGACCATGGG
GTGGCCACGGCCGGGCCGAGCCCTCGTGGCAGTCAAAGCCTTGCTGGTCTTGTCGCTGCT
CCAGGTGCCCGCGCAGGCAGTGGTACGGGCCGTGCTGGAAGACAACTCGAGCTCGGTGGA
CTTTGCGGATCTGCCGGCGCTGTTCGGCGTCCCCCTGGCCCCCGAGGGCATACGGGGCTA
CCTGATGGAGGTCAAGCCAGCCAACGCGTGCCATCCCATCGAGGCCCCGCGACTGGGCAA
CCGCTCTCTGGGCTCCATTGCGCTGATCCGCCGCTACGACTGCACCTTCGACCTCAAGGT
GCTGAACGCCCAGCGCGCCGGCTTCGAGGCGGCCATCGTGCACAACGTCCACTCCGACGA
CCTCGTGAGCATGACCCACGTCTACGAGGACTTGAGGGGCCAGATCGCCATCCCCTCAGT
GTTCGTGAGCGAGGCCGCCTCGCAGGACCTGCGGGTCATCCTGGGCTGCAACAAGTCGGC
CCACGCGCTGCTCCTGCCCGACGACCCACCGTGCCACGACCTGGGCTGTCACCCCGTGCT
GACCGTGTCCTGGGTGCTGGGCTGTACCCTGGCCCTGGTCGTATCAGCCTTCTTTGTCCT
GAACCACCTGTGGCTCTGGGCCCAGGCCTGCTGCAGCCACAGACGGCCGGTGAAGACGTC
TACCTGCCAGAAGGCCCAGGTCCGCACCTTCACGTGGCACAACGACCTGTGTGCCATCTG
CCTGGATGAGTACGAGGAGGGCGACCAACTCAAGATCCTGCCCTGCTCCCACACCTACCA
CTGCAAATGCATTGACCCCTGGTTCTCCCAAGCCCCCCGGCGCTCCTGCCCCGTGTGCAA
ACAGTCGGTGGCCGCCACAGAAGACAGCTTTGACTCCACCACCTACAGCTTCAGGGACGA
GGACCCCTCCCTACCGGGCCACCGGCCCCCCATCTGGGCCATTCAAGTCCAGCTACGCTC
CCGGAGGCTGGAGCTGCTGGGCCGCGCCAGTCCCCACTGCCACTGCAGCACCACGTCCCT
GGAGGCAGAGTATACCACTGTCTCCTCAGCCCCTCCTGAGGCCCCTGGTCAGGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT