Restriction map for
pFN21AE4082
##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
AhdI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BbeI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrGI BstBI CelII
ClaI CpoI DraI Eam1105I EarI
Eco47III EcoRI EcoRV FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MfeI
MslI MspA1I MunI NarI NdeI
NheI NotI NruI NspI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tsp509I XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 870 GACGTC GACGT^C
AccIII 15 TCCGGA T^CCGGA
AflIII 419 ACGCGT A^C[GA][CT]GT
Alw44I 553 GTGCAC G^TGCAC
AlwNI 658 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1270 CCTGAGG CC^T[ACGT]AGG
ApaI 833 GGGCCC GGGCC^C
ApaLI 553 GTGCAC G^TGCAC
AspI 756 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 279 GGTGCC G^G[CT][GA]CC
BbrPI 906 CACGTG CAC^GTG
BbvCI 1259 CCTCAGC CCTCAGC(-5/-2)
BcgI 918 CGACCTGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 235 GCCCTCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 906 CACGTG [CT]AC^GT[GA]
BsaBI 618 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 129 GAGACC GGTCTC(1/5)
BsaWI 15 TCCGGA [AT]^CCGG[AT]
BseAI 15 TCCGGA T^CCGGA
BsgI 495 CTGCAC GTGCAG(16/14)
BspEI 15 TCCGGA T^CCGGA
BsrDI 471 CATTGC GCAATG(2/0)
BssHII 528 GCGCGC G^CGCGC
Bst1107I 1245 GTATAC GTA^TAC
BstEII 204 GGTGACC G^GT[ACGT]ACC
BstPI 204 GGTGACC G^GT[ACGT]ACC
BstZ17I 1245 GTATAC GTA^TAC
Bsu36I 1270 CCTGAGG CC^T[ACGT]AGG
DraIII 745 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 661 GACCTGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1270 CCTGAGG CC^T[ACGT]AGG
EcoNI 396 CCTGATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 204 GGTGACC G^GT[ACGT]ACC
EcoT14I 209 CCATGG C^C[AT][AT]GG
EcoT22I 1002 ATGCAT ATGCA^T
HinfI 1087 GACTC G^A[ACGT]TC
MamI 618 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 419 ACGCGT A^CGCGT
MroI 15 TCCGGA T^CCGGA
MseI 25 TTAA T^TAA
NcoI 209 CCATGG C^CATGG
NsiI 1002 ATGCAT ATGCA^T
PaeR7I 321 CTCGAG C^TCGAG
PflFI 756 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1087 GACTC GAGTC(4/5)
PmaCI 906 CACGTG CAC^GTG
PmlI 906 CACGTG CAC^GTG
PshAI 588 GACCCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 324 GAGCTC GAGCT^C
SfaNI 140 GATGC GCATC(5/9)
StyI 209 CCATGG C^C[AT][AT]GG
Tru9I 25 TTAA T^TAA
Tth111I 756 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 114 CCACAGGCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 321 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 361 GGCGTC G[GA]^CG[CT]C
870 GACGTC
AvaI 321 CTCGAG C^[CT]CG[GA]G
376 CCCGAG
BalI 111 TGGCCA TGG^CCA
217 TGGCCA
BfaI 32 CTAG C^TAG
179 CTAG
BsaHI 361 GGCGTC G[GA]^CG[CT]C
870 GACGTC
BseRI 1257 CTCCTC GAGGAG(10/8)
949 GAGGAG
BsiEI 223 CGGCCG CG[GA][CT]^CG
859 CGGCCG
BsmAI 129 GAGAC GTCTC(1/5)
1255 GTCTC
BsoBI 321 CTCGAG C^[CT]CG[GA]G
376 CCCGAG
BspMI 662 ACCTGC ACCTGC(4/8)
877 ACCTGC
BsrBI 4 CCGCTC CCGCTC(-3/-3)
456 CCGCTC
BssSI 238 CTCGTG CACGAG(-5/-1)
577 CTCGTG
BstAPI 21 GCACTTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
657 GCAGGACCTGC
BstXI 929 CCATCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1226 CCACGTCCCTGG
BstYI 342 GGATCT [GA]^GATC[CT]
968 AGATCC
DsaI 209 CCATGG C^C[GA][CT]GG
220 CCACGG
EagI 223 CGGCCG C^GGCCG
859 CGGCCG
EclXI 223 CGGCCG C^GGCCG
859 CGGCCG
Eco52I 223 CGGCCG C^GGCCG
859 CGGCCG
HaeII 351 GGCGCT [GA]GCGC^[CT]
1034 GGCGCT
Hin1I 361 GGCGTC G[GA]^CG[CT]C
870 GACGTC
MaeI 32 CTAG C^TAG
179 CTAG
MflI 342 GGATCT [GA]^GATC[CT]
968 AGATCC
MluNI 111 TGGCCA TGG^CCA
217 TGGCCA
MscI 111 TGGCCA TGG^CCA
217 TGGCCA
NaeI 348 GCCGGC GCC^GGC
532 GCCGGC
NgoMIV 348 GCCGGC G^CCGGC
532 GCCGGC
PflMI 209 CCATGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
729 CCACGACCTGG
PstI 846 CTGCAG CTGCA^G
1218 CTGCAG
StuI 149 AGGCCT AGG^CCT
839 AGGCCT
Van91I 209 CCATGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
729 CCACGACCTGG
XhoII 342 GGATCT [GA]^GATC[CT]
968 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AspHI AvaII BanII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PpuMI RsaI Sau3AI Sau96I
ScrFI SfcI SmlI TaqI TseI
Tsp45I TspRI TthHB8I