Restriction map for
pFN21AE4082
(Linear) (Six-base) MAPSORT of: KIEE4082.seq Check: 8349 from: 1 to: 1287
KIEE4082.orf
With 182 enzymes: SgfI *
January 29, 2014 11:45 ..
AatII G_ACGT'C
Cuts at: 0 874 1287
Size: 874 413
AccI GT'mk_AC
Cuts at: 0 596 874 1246 1287
Size: 596 278 372 41
Fragments arranged by size:
596 372 278 41
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1089 1287
Size: 1089 198
AflIII A'CryG_T
Cuts at: 0 419 1287
Size: 419 868
AlwNI CAG_nnn'CTG
Cuts at: 0 663 1287
Size: 663 624
ApaI G_GGCC'C
Cuts at: 0 837 1287
Size: 837 450
ApaLI G'TGCA_C
Cuts at: 0 553 1287
Size: 553 734
AvaI C'yCGr_G
Cuts at: 0 321 376 1287
Size: 321 55 911
Fragments arranged by size:
911 321 55
BanI G'GyrC_C
Cuts at: 0 279 1287
Size: 279 1008
BanII G_rGCy'C
Cuts at: 0 237 328 469 837 1287
Size: 237 91 141 368 450
Fragments arranged by size:
450 368 237 141 91
BbsI GAAGACnn'nnnn_
Cuts at: 0 320 874 1082 1287
Size: 320 554 208 205
Fragments arranged by size:
554 320 208 205
BbvCI CC'TCA_GC
Cuts at: 0 1260 1287
Size: 1260 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 907 941 1287
Size: 907 34 346
Fragments arranged by size:
907 346 34
BfrBI ATG'CAT
Cuts at: 0 1004 1287
Size: 1004 283
BglI GCCn_nnn'nGGC
Cuts at: 0 241 1287
Size: 241 1046
Bme1580I G_kGCm'C
Cuts at: 0 284 557 837 1287
Size: 284 273 280 450
Fragments arranged by size:
450 284 280 273
BmgBI CAC'GTC
Cuts at: 0 594 1229 1287
Size: 594 635 58
Fragments arranged by size:
635 594 58
BmrI ACTGGGnnnn_n'
Cuts at: 0 43 457 1287
Size: 43 414 830
Fragments arranged by size:
830 414 43
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 669 1137 1287
Size: 669 468 150
Fragments arranged by size:
669 468 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 259 1204 1255 1287
Size: 152 107 945 51 32
Fragments arranged by size:
945 152 107 51 32
Bpu10I CC'TnA_GC
Cuts at: 0 1260 1287
Size: 1260 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 627 949 1287
Size: 493 134 322 338
Fragments arranged by size:
493 338 322 134
BsaI GGTCTCn'nnnn_
Cuts at: 0 123 1287
Size: 123 1164
BsaAI yAC'GTr
Cuts at: 0 908 1287
Size: 908 379
BsaBI GATnn'nnATC
Cuts at: 0 622 1287
Size: 622 665
BsaHI Gr'CG_yC
Cuts at: 0 362 871 1287
Size: 362 509 416
Fragments arranged by size:
509 416 362
BsaWI w'CCGG_w
Cuts at: 0 15 1287
Size: 15 1272
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 550 580 1228 1258 1287
Size: 550 30 648 30 29
Fragments arranged by size:
648 550 30 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 964 1248 1287
Size: 964 284 39
Fragments arranged by size:
964 284 39
BseYI C'CCAG_C
Cuts at: 0 524 771 1191 1287
Size: 524 247 420 96
Fragments arranged by size:
524 420 247 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 1287
Size: 480 807
BsiEI CG_ry'CG
Cuts at: 0 226 862 1287
Size: 226 636 425
Fragments arranged by size:
636 425 226
BsiHKAI G_wGCw'C
Cuts at: 0 23 328 557 1287
Size: 23 305 229 730
Fragments arranged by size:
730 305 229 23
Bsp1286I G_dGCh'C
Cuts at: 0 23 237 284 328 469 557 837 1287
Size: 23 214 47 44 141 88 280 450
Fragments arranged by size:
450 280 214 141 88 47 44 23
BspEI T'CCGG_A
Cuts at: 0 15 1287
Size: 15 1272
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 671 886 1287
Size: 671 215 401
Fragments arranged by size:
671 401 215
BsrBI CCG'CTC
Cuts at: 0 6 458 1287
Size: 6 452 829
Fragments arranged by size:
829 452 6
BsrDI GCAATG_nn'
Cuts at: 0 470 1287
Size: 470 817
BsrFI r'CCGG_y
Cuts at: 0 348 532 861 1136 1287
Size: 348 184 329 275 151
Fragments arranged by size:
348 329 275 184 151
BssHII G'CGCG_C
Cuts at: 0 528 1287
Size: 528 759
BssSI C'ACGA_G
Cuts at: 0 238 577 1287
Size: 238 339 710
Fragments arranged by size:
710 339 238
BstAPI GCAn_nnn'nTGC
Cuts at: 0 27 663 1287
Size: 27 636 624
Fragments arranged by size:
636 624 27
BstEII G'GTnAC_C
Cuts at: 0 204 1287
Size: 204 1083
BstXI CCAn_nnnn'nTGG
Cuts at: 0 936 1233 1287
Size: 936 297 54
Fragments arranged by size:
936 297 54
BstYI r'GATC_y
Cuts at: 0 342 968 1287
Size: 342 626 319
Fragments arranged by size:
626 342 319
BstZ17I GTA'TAC
Cuts at: 0 1247 1287
Size: 1247 40
Bsu36I CC'TnA_GG
Cuts at: 0 1271 1287
Size: 1271 16
BtgI C'CryG_G
Cuts at: 0 209 220 1287
Size: 209 11 1067
Fragments arranged by size:
1067 209 11
BtsI GCAGTG_nn'
Cuts at: 0 61 299 993 1209 1215 1287
Size: 61 238 694 216 6 72
Fragments arranged by size:
694 238 216 72 61 6
DraIII CAC_nnn'GTG
Cuts at: 0 750 1287
Size: 750 537
DrdI GACnn_nn'nnGTC
Cuts at: 0 667 1287
Size: 667 620
EaeI y'GGCC_r
Cuts at: 0 53 111 217 223 545 859 1064 1287
Size: 53 58 106 6 322 314 205 223
Fragments arranged by size:
322 314 223 205 106 58 53 6
EagI C'GGCC_G
Cuts at: 0 223 859 1287
Size: 223 636 428
Fragments arranged by size:
636 428 223
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 472 1287
Size: 472 815
EcoICRI GAG'CTC
Cuts at: 0 326 1287
Size: 326 961
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 259 1089 1204 1255 1287
Size: 152 107 830 115 51 32
Fragments arranged by size:
830 152 115 107 51 32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 400 1287
Size: 400 887
EcoO109I rG'GnC_Cy
Cuts at: 0 45 119 175 438 660 1116 1275 1287
Size: 45 74 56 263 222 456 159 12
Fragments arranged by size:
456 263 222 159 74 56 45 12
HaeII r_GCGC'y
Cuts at: 0 355 1038 1287
Size: 355 683 249
Fragments arranged by size:
683 355 249
MluI A'CGCG_T
Cuts at: 0 419 1287
Size: 419 868
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 593 1287
Size: 112 481 694
Fragments arranged by size:
694 481 112
MscI TGG'CCA
Cuts at: 0 113 219 1287
Size: 113 106 1068
Fragments arranged by size:
1068 113 106
NaeI GCC'GGC
Cuts at: 0 350 534 1287
Size: 350 184 753
Fragments arranged by size:
753 350 184
NcoI C'CATG_G
Cuts at: 0 209 1287
Size: 209 1078
NgoMIV G'CCGG_C
Cuts at: 0 348 532 1287
Size: 348 184 755
Fragments arranged by size:
755 348 184
NsiI A_TGCA'T
Cuts at: 0 1006 1287
Size: 1006 281
PasI CC'CwG_GG
Cuts at: 0 124 1287
Size: 124 1163
PflMI CCAn_nnn'nTGG
Cuts at: 0 215 735 1287
Size: 215 520 552
Fragments arranged by size:
552 520 215
PfoI T'CCnGG_A
Cuts at: 0 1174 1287
Size: 1174 113
PmlI CAC'GTG
Cuts at: 0 908 1287
Size: 908 379
PpuMI rG'GwC_Cy
Cuts at: 0 45 175 660 1116 1287
Size: 45 130 485 456 171
Fragments arranged by size:
485 456 171 130 45
PshAI GACnn'nnGTC
Cuts at: 0 592 1287
Size: 592 695
PspOMI G'GGCC_C
Cuts at: 0 833 1287
Size: 833 454
PspXI vC'TCGA_Gb
Cuts at: 0 321 1287
Size: 321 966
PstI C_TGCA'G
Cuts at: 0 850 1222 1287
Size: 850 372 65
Fragments arranged by size:
850 372 65
SacI G_AGCT'C
Cuts at: 0 328 1287
Size: 328 959
SanDI GG'GwC_CC
Cuts at: 0 45 1287
Size: 45 1242
SfcI C'TryA_G
Cuts at: 0 846 1098 1218 1287
Size: 846 252 120 69
Fragments arranged by size:
846 252 120 69
SmlI C'TyrA_G
Cuts at: 0 321 508 606 964 1287
Size: 321 187 98 358 323
Fragments arranged by size:
358 323 321 187 98
StuI AGG'CCT
Cuts at: 0 151 841 1287
Size: 151 690 446
Fragments arranged by size:
690 446 151
StyI C'CwwG_G
Cuts at: 0 209 1287
Size: 209 1078
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 756 1287
Size: 756 531
Tth111I GACn'n_nGTC
Cuts at: 0 759 1287
Size: 759 528
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 121 1287
Size: 121 1166
XhoI C'TCGA_G
Cuts at: 0 321 1287
Size: 321 966
ZraI GAC'GTC
Cuts at: 0 872 1287
Size: 872 415
Enzymes that do cut:
AatII AccI AcuI AflIII AlwNI ApaI ApaLI AvaI
BanI BanII BbsI BbvCI BcgI BfrBI BglI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtsI DraIII DrdI EaeI EagI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII MluI MmeI MscI NaeI NcoI
NgoMIV NsiI PasI PflMI PfoI PmlI PpuMI PshAI
PspOMI PspXI PstI SacI SanDI SfcI SmlI StuI
StyI TaqII Tth111I XcmI XhoI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BciVI BclI BglII BlpI BmtI BsiWI
BsmI BsmBI BspHI BsrGI BstBI BtgZI ClaI CspCI
DraI EarI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MfeI MslI
MspA1I NarI NdeI NheI NotI NruI NspI PacI
PciI PmeI PpiI PsiI PsrI PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TatI TstI XbaI XmaI XmnI