ASB11 ORF clone pFN21AE4058

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Halotag ORF Clone pFN21AE4058

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Clone info.

Species

human

Clone Name

pFN21AE4058 Sequence (with vector) Restriction Map

Product ID

FHC27303

ORF Sequence

DNA sequence (969 bp)
Protein sequence (323 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4058

Symbol

ASB11

Description

ankyrin repeat and SOCS box containing 11, E3 ubiquitin protein ligase, transcript variant 1

Original Clone ID

cp94058

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 969 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 323 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS14164.1 3.2e-107 100.0 ASB11 CCDS35209.1 5.3e-87 100.0 ASB11 CCDS56596.1 1.9e-72 94.7 ASB11 CCDS3827.1 2.7e-56 53.8 ASB5 CCDS35208.1 1.2e-45 55.3 ASB9 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR002110 66 95 PF00023 Ankyrin repeat IPR002110 102 125 PF00023 Ankyrin repeat IPR002110 198 223 PF00023 Ankyrin repeat IPR002110 229 258 PF00023 Ankyrin repeat IPR001496 284 323 PF07525 SOCS protein HMMSmart IPR002110 64 93 SM00248 Ankyrin repeat IPR002110 97 126 SM00248 Ankyrin repeat IPR002110 130 159 SM00248 Ankyrin repeat IPR002110 162 191 SM00248 Ankyrin repeat IPR002110 195 224 SM00248 Ankyrin repeat IPR002110 227 256 SM00248 Ankyrin repeat IPR001496 284 323 SM00969 SOCS protein ProfileScan IPR002110 64 96 PS50088 Ankyrin repeat IPR020683 64 280 PS50297 Ankyrin repeat-containing domain IPR002110 97 129 PS50088 Ankyrin repeat IPR002110 130 162 PS50088 Ankyrin repeat IPR002110 162 190 PS50088 Ankyrin repeat IPR002110 195 223 PS50088 Ankyrin repeat IPR002110 230 259 PS50088 Ankyrin repeat IPR001496 273 323 PS50225 SOCS protein
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 16 TMFATFFFFKLLIKVFLALLTHF 38 PRIMARY 23 2 134 PLFNACCSGSAACVNVLLEFGAK 156 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_543149 323 300626 100.0 100.0 NP_001012428 302 300626 100.0 81.4 NP_001188512 306 300626 94.7 100.0 NP_543150 329 615050 53.8 99.7 XP_005262816 329 615050 53.8 99.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4058 Download

>KIEE4058  969 bp
ATGGAAGATGGTCCTGTTTTCTATGGCTTTAAAAACATTTTTATTACAATGTTTGCTACG
TTTTTTTTCTTTAAGCTTTTAATTAAAGTTTTTTTGGCTCTCCTAACCCATTTCTATATC
GTCAAAGGAAATAGAAAAGAAGCGGCTAGGATAGCAGAAGAGATCTATGGTGGAATTTCA
GATTGCTGGGCTGATCGATCCCCACTTCATGAAGCTGCAGCTCAGGGGCGCTTACTGGCC
CTTAAAACTTTAATTGCACAAGGTGTCAATGTGAACCTTGTGACAATTAACCGGGTGTCT
TCTCTCCACGAGGCATGCCTTGGAGGTCACGTGGCCTGTGCCAAAGCCTTATTGGAAAAT
GGTGCACACGTCAATGGAGTGACAGTTCACGGAGCCACACCCCTCTTCAATGCTTGCTGC
AGCGGCAGTGCTGCATGTGTCAATGTGCTGCTGGAGTTCGGAGCCAAGGCCCAGTTGGAG
GTGCACCTGGCCTCGCCCATCCATGAGGCAGTGAAGAGAGGTCACAGAGAGTGCATGGAG
ATCCTGCTGGCAAATAATGTTAACATTGACCATGAGGTGCCTCAGCTCGGAACTCCCCTA
TATGTGGCCTGCACCTACCAGAGGGTAGACTGTGTGAAGAAACTTCTAGAATTAGGAGCC
AGTGTCGACCATGGCCAGTGGCTGGACACCCCACTCCATGCTGCAGCGAGGCAGTCCAAT
GTGGAGGTCATCCACCTGCTAACCGACTATGGAGCTAACCTGAAGCGTAGAAATGCTCAG
GGCAAAAGTGCGCTTGATCTGGCGGCTCCAAAAAGCAGCGTGGAGCAGGCACTCTTGCTC
CGTGAAGGCCCACCTGCTCTTTCCCAGCTCTGCCGCCTGTGTGTCCGGAAGTGTCTCGGT
CGAGCATGTCATCAAGCCATCCACAAGCTACATCTGCCAGAGCCACTCGAACGATTCCTC
CTATACCAA

ORF Protein

Cloned ORF protein sequence for pFN21AE4058 Download

>KIEE4058  323 aa
MEDGPVFYGFKNIFITMFATFFFFKLLIKVFLALLTHFYIVKGNRKEAARIAEEIYGGIS
DCWADRSPLHEAAAQGRLLALKTLIAQGVNVNLVTINRVSSLHEACLGGHVACAKALLEN
GAHVNGVTVHGATPLFNACCSGSAACVNVLLEFGAKAQLEVHLASPIHEAVKRGHRECME
ILLANNVNIDHEVPQLGTPLYVACTYQRVDCVKKLLELGASVDHGQWLDTPLHAAARQSN
VEVIHLLTDYGANLKRRNAQGKSALDLAAPKSSVEQALLLREGPPALSQLCRLCVRKCLG
RACHQAIHKLHLPEPLERFLLYQ

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4058 Download

>pFN21AE4058  5674 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAAGATGGTCCTGTTTTCTATGGCTTTAAAAA
CATTTTTATTACAATGTTTGCTACGTTTTTTTTCTTTAAGCTTTTAATTAAAGTTTTTTT
GGCTCTCCTAACCCATTTCTATATCGTCAAAGGAAATAGAAAAGAAGCGGCTAGGATAGC
AGAAGAGATCTATGGTGGAATTTCAGATTGCTGGGCTGATCGATCCCCACTTCATGAAGC
TGCAGCTCAGGGGCGCTTACTGGCCCTTAAAACTTTAATTGCACAAGGTGTCAATGTGAA
CCTTGTGACAATTAACCGGGTGTCTTCTCTCCACGAGGCATGCCTTGGAGGTCACGTGGC
CTGTGCCAAAGCCTTATTGGAAAATGGTGCACACGTCAATGGAGTGACAGTTCACGGAGC
CACACCCCTCTTCAATGCTTGCTGCAGCGGCAGTGCTGCATGTGTCAATGTGCTGCTGGA
GTTCGGAGCCAAGGCCCAGTTGGAGGTGCACCTGGCCTCGCCCATCCATGAGGCAGTGAA
GAGAGGTCACAGAGAGTGCATGGAGATCCTGCTGGCAAATAATGTTAACATTGACCATGA
GGTGCCTCAGCTCGGAACTCCCCTATATGTGGCCTGCACCTACCAGAGGGTAGACTGTGT
GAAGAAACTTCTAGAATTAGGAGCCAGTGTCGACCATGGCCAGTGGCTGGACACCCCACT
CCATGCTGCAGCGAGGCAGTCCAATGTGGAGGTCATCCACCTGCTAACCGACTATGGAGC
TAACCTGAAGCGTAGAAATGCTCAGGGCAAAAGTGCGCTTGATCTGGCGGCTCCAAAAAG
CAGCGTGGAGCAGGCACTCTTGCTCCGTGAAGGCCCACCTGCTCTTTCCCAGCTCTGCCG
CCTGTGTGTCCGGAAGTGTCTCGGTCGAGCATGTCATCAAGCCATCCACAAGCTACATCT
GCCAGAGCCACTCGAACGATTCCTCCTATACCAAGTTTAAACGAATTCGGGCTCGGTACC
CGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCC
GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGG
CGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGA
ATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTT
CAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATC
GAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTG
ACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAG
ACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAA
ATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATAT
TGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCG
GCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA
GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT
GAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGT
GGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT
TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATG
ACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA
CTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT
CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG
CGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAA
CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCA
GGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCC
GGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT
ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATC
GCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACG
CCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATA
ACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA
GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGT
AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG
CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT
TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG
CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT
AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCG
GAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGG
CAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTC
ATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTT
TTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGA
TTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCG
GCCACGATGCGTCCGGCGTAGAGGATCGAGATCT