Restriction map for
pFN21AE4058
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AfaI AflII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanII BbeI
BcgI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BseRI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
CpoI DraII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HgaI Hin1I
HphI KasI KpnI KspI MamI
MfeI MluI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PaeR7I PflFI
PinAI PleI PmeI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsaI
RsrII SacI SacII SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccIII 884 TCCGGA T^CCGGA
AcsI 173 GAATTT [GA]^AATT[CT]
AflIII 366 ACACGT A^C[GA][CT]GT
AlwNI 676 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 173 GAATTT [GA]^AATT[CT]
AvaII 10 GGTCC G^G[AT]CC
BalI 672 TGGCCA TGG^CCA
BanI 576 GGTGCC G^G[CT][GA]CC
BbrPI 328 CACGTG CAC^GTG
BbsI 297 GTCTTC GAAGAC(2/6)
BbvCI 580 CCTCAGC CCTCAGC(-5/-2)
BcnI 291 CCGGG CC^[GC]GG
BglII 161 AGATCT A^GATCT
BpmI 451 CTGGAG CTGGAG(16/14)
BpuAI 297 GTCTTC GAAGAC(2/6)
BsaAI 328 CACGTG [CT]AC^GT[GA]
BsaWI 884 TCCGGA [AT]^CCGG[AT]
BseAI 884 TCCGGA T^CCGGA
BsgI 609 CTGCAC GTGCAG(16/14)
BsiEI 897 CGGTCG CG[GA][CT]^CG
BsmAI 893 GTCTC GTCTC(1/5)
BspDI 194 ATCGAT AT^CGAT
BspEI 884 TCCGGA T^CCGGA
BspHI 207 TCATGA T^CATGA
BssSI 307 CACGAG CACGAG(-5/-1)
BstNI 486 CCTGG CC^[AT]GG
ClaI 194 ATCGAT AT^CGAT
DraI 28 TTTAAA TTT^AAA
DraIII 257 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 669 CCATGG C^C[GA][CT]GG
EaeI 672 TGGCCA [CT]^GGCC[GA]
EcoNI 614 CCTACCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 486 CCTGG ^CC[AT]GG
HaeII 227 GGCGCT [GA]GCGC^[CT]
HindIII 73 AAGCTT A^AGCTT
HinfI 953 GATTC G^A[ACGT]TC
HpaI 559 GTTAAC GTT^AAC
MluNI 672 TGGCCA TGG^CCA
MroI 884 TCCGGA T^CCGGA
MscI 672 TGGCCA TGG^CCA
MslI 367 CACGTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 420 CAGCGG C[AC]G^C[GT]G
MvaI 486 CCTGG CC^[AT]GG
NciI 291 CCGGG CC^[GC]GG
NcoI 669 CCATGG C^CATGG
PacI 79 TTAATTAA TTAAT^TAA
PflMI 675 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 328 CACGTG CAC^GTG
PmlI 328 CACGTG CAC^GTG
RcaI 207 TCATGA T^CATGA
SalI 664 GTCGAC G^TCGAC
SphI 313 GCATGC GCATG^C
TfiI 953 GATTC G^A[AT]TC
Van91I 675 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 645 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 625 GTAGAC GT^[AC][GT]AC
664 GTCGAC
Alw44I 362 GTGCAC G^TGCAC
481 GTGCAC
AlwI 197 GATCC GGATC(4/5)
540 GATCC
ApaLI 362 GTGCAC G^TGCAC
481 GTGCAC
AspHI 362 GTGCAC G[AT]GC[AT]^C
481 GTGCAC
BfaI 146 CTAG C^TAG
646 CTAG
BmyI 362 GTGCAC G[AGT]GC[ACT]^C
481 GTGCAC
BsiHKAI 362 GTGCAC G[AT]GC[AT]^C
481 GTGCAC
Bsp1286I 362 GTGCAC G[AGT]GC[ACT]^C
481 GTGCAC
BspMI 734 ACCTGC ACCTGC(4/8)
852 ACCTGC
BssKI 291 CCGGG ^CC[ACGT]GG
486 CCTGG
BstYI 161 AGATCT [GA]^GATC[CT]
539 AGATCC
CfoI 228 GCGC GCG^C
790 GCGC
HapII 291 CCGG C^CGG
885 CCGG
HhaI 228 GCGC GCG^C
790 GCGC
HinP1I 228 GCGC G^CGC
790 GCGC
HincII 559 GTTAAC GT[CT]^[GA]AC
664 GTCGAC
HindII 559 GTTAAC GT[CT]^[GA]AC
664 GTCGAC
HpaII 291 CCGG C^CGG
885 CCGG
MaeI 146 CTAG C^TAG
646 CTAG
MflI 161 AGATCT [GA]^GATC[CT]
539 AGATCC
MspI 291 CCGG C^CGG
885 CCGG
ScrFI 291 CCGGG CC^[ACGT]GG
486 CCTGG
XcmI 341 CCAAAGCCTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
464 CCAAGGCCCAGTTGG
XhoII 161 AGATCT [GA]^GATC[CT]
539 AGATCC
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BsrI BstF5I Cac8I Cfr13I
DdeI DpnII EarI EcoT14I Fnu4HI
FokI HaeIII ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MseI
MwoI NdeII NlaIII NlaIV NspI
PstI Sau3AI Sau96I SfcI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I