Restriction map for
pFN21AE4058
(Circular) (Six-base) MAPSORT of: pFN21AE4058.seq Check: 5204 from: 1 to: 5674
pFN21AE4058 5674 bp
With 182 enzymes: SgfI *
March 28, 2012 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2748 2866 2748
Size: 118 5556
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3484 278
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2631 2670 3017 1937
Size: 694 39 347 4594
Fragments arranged by size:
4594 694 347 39
Acc65I G'GTAC_C
Cuts at: 2995 2995
Size: 5674
AclI AA'CG_TT
Cuts at: 1563 3801 4174 1563
Size: 2238 373 3063
Fragments arranged by size:
3063 2238 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2786 3737 5121 5389 1632
Size: 1154 951 1384 268 1917
Fragments arranged by size:
1917 1384 1154 951 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5477
AflIII A'CryG_T
Cuts at: 1203 2371 4573 5550 1203
Size: 1168 2202 977 1327
Fragments arranged by size:
2202 1327 1168 977
AgeI A'CCGG_T
Cuts at: 4505 4505
Size: 5674
AhdI GACnn_n'nnGTC
Cuts at: 4403 4403
Size: 5674
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5674
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5674
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2373 2405 2946 2978 2373
Size: 32 541 32 5069
Fragments arranged by size:
5069 541 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2686 3417 4989 2686
Size: 731 1572 3371
Fragments arranged by size:
3371 1572 731
ApaLI G'TGCA_C
Cuts at: 2367 2486 3731 4887 2367
Size: 119 1245 1156 3154
Fragments arranged by size:
3154 1245 1156 119
ApoI r'AATT_y
Cuts at: 1493 2178 2983 3209 3361 5470 1493
Size: 685 805 226 152 2109 1697
Fragments arranged by size:
2109 1697 805 685 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4228 160
Size: 872 3196 1606
Fragments arranged by size:
3196 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
AvaI C'yCGr_G
Cuts at: 1870 1943 2999 1870
Size: 73 1056 4545
Fragments arranged by size:
4545 1056 73
BamHI G'GATC_C
Cuts at: 3004 3371 3004
Size: 367 5307
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2581 2995 4450 618
Size: 325 217 627 794 414 1455 1842
Fragments arranged by size:
1842 1455 794 627 414 325 217
BanII G_rGCy'C
Cuts at: 1391 2993 1391
Size: 1602 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2295 928
Size: 971 396 4307
Fragments arranged by size:
4307 971 396
BbvCI CC'TCA_GC
Cuts at: 2586 2586
Size: 5674
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3865 3899 1903
Size: 34 1928 34 3678
Fragments arranged by size:
3678 1928 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3566 4776 3566
Size: 1210 4464
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5674
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4285 136
Size: 107 122 71 631 605 2613 1525
Fragments arranged by size:
2613 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2166 5669 2166
Size: 3503 2171
BlpI GC'TnA_GC
Cuts at: 3091 3091
Size: 5674
Bme1580I G_kGCm'C
Cuts at: 1790 2371 2490 3735 4891 1790
Size: 581 119 1245 1156 2573
Fragments arranged by size:
2573 1245 1156 581 119
BmgBI CAC'GTC
Cuts at: 1318 2374 1318
Size: 1056 4618
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2470 3405 4363
Size: 416 53 83 399 24 1053 935 958
Cuts at: 4363 442
Size: 1753
Fragments arranged by size:
1753 1053 958 935 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2477 4334 1239
Size: 1238 1857 2579
Fragments arranged by size:
2579 1857 1238
Bpu10I CC'TnA_GC
Cuts at: 2226 2586 2781 2226
Size: 360 195 5119
Fragments arranged by size:
5119 360 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3799 4664 4962 3799
Size: 865 298 4511
Fragments arranged by size:
4511 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4337 882
Size: 617 2838 2219
Fragments arranged by size:
2838 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2335 3465 493
Size: 711 1131 1130 2702
Fragments arranged by size:
2702 1131 1130 711
BsaBI GATnn'nnATC
Cuts at: 5668 5668
Size: 5674
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3481 3863 4498 275
Size: 53 83 186 936 1948 382 635 1451
Fragments arranged by size:
1948 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2889 4107 4505 4779 4926 5337 2889
Size: 1218 398 274 147 411 3226
Fragments arranged by size:
3226 1218 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2319 2349 2373 2403 2652
Size: 30 165 30 916 30 24 30 249
Cuts at: 2652 2682 2826 2856 2946 2976 1178
Size: 30 144 30 90 30 3876
Fragments arranged by size:
3876 916 249 165 144 90 30 30
30 30 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5674
BseYI C'CCAG_C
Cuts at: 2190 2868 4877 5373 2190
Size: 678 2009 496 2491
Fragments arranged by size:
2491 2009 678 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2599 1163
Size: 573 863 4238
Fragments arranged by size:
4238 863 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2905 3124 3885 4913 1540
Size: 275 186 904 219 761 1028 2301
Fragments arranged by size:
2301 1028 904 761 275 219 186
BsiHKAI G_wGCw'C
Cuts at: 2371 2490 3735 3820 4891 2371
Size: 119 1245 85 1071 3154
Fragments arranged by size:
3154 1245 1071 119 85
BsmI GAATG_Cn'
Cuts at: 3185 3278 3185
Size: 93 5581
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2371 2490 2993 3735 3820 4891 1391
Size: 399 581 119 503 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 581 503 399 119 85
BspEI T'CCGG_A
Cuts at: 2889 5337 2889
Size: 2448 3226
BspHI T'CATG_A
Cuts at: 2212 3563 5293 2212
Size: 1351 1730 2593
Fragments arranged by size:
2593 1730 1351
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2748 2866 3029 844
Size: 1904 118 163 3489
Fragments arranged by size:
3489 1904 163 118
BsrBI CCG'CTC
Cuts at: 1394 1823 3561 1394
Size: 429 1738 3507
Fragments arranged by size:
3507 1738 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4169 4343 65
Size: 1183 2921 174 1396
Fragments arranged by size:
2921 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4318 4505 1700
Size: 110 2508 187 2869
Fragments arranged by size:
2869 2508 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5674
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5674
BssSI C'ACGA_G
Cuts at: 2312 3734 4746 2312
Size: 1422 1012 3240
Fragments arranged by size:
3240 1422 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5674
BstBI TT'CG_AA
Cuts at: 4478 4478
Size: 5674
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5674
BstYI r'GATC_y
Cuts at: 1972 2166 2544 3004 3371 3756 3773 5225 5311
Size: 194 378 460 367 385 17 1452 86
Cuts at: 5311 5323 5669 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 460 385 378 367 346 194
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2674 513
Size: 552 939 670 3513
Fragments arranged by size:
3513 939 670 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4935
Fragments arranged by size:
4935 683 56
BtsI GCAGTG_nn'
Cuts at: 2437 2520 3190 3984 4004 2437
Size: 83 670 794 20 4107
Fragments arranged by size:
4107 794 670 83 20
ClaI AT'CG_AT
Cuts at: 1554 2200 1554
Size: 646 5028
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5639
DraI TTT'AAA
Cuts at: 2035 2978 2035
Size: 943 4731
DraIII CAC_nnn'GTG
Cuts at: 2267 2267
Size: 5674
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4681 809
Size: 507 3365 1802
Fragments arranged by size:
3365 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2677 3121 4010
Size: 54 1425 50 275 18 847 444 889
Cuts at: 4010 5639 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 847 444 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3121 1537
Size: 275 1309 4090
Fragments arranged by size:
4090 1309 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2157 2414 2513 3604 1353
Size: 804 257 99 1091 3423
Fragments arranged by size:
3423 1091 804 257 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4261 4635 4781 387
Size: 1064 138 2672 374 146 1280
Fragments arranged by size:
2672 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2477 2786 3737 4334 5121 5389 1239
Size: 393 845 309 951 597 787 268 1524
Fragments arranged by size:
1524 951 845 787 597 393 309 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2623 2623
Size: 5674
EcoRI G'AATT_C
Cuts at: 2983 3361 2983
Size: 378 5296
FspI TGC'GCA
Cuts at: 4180 4180
Size: 5674
HaeII r_GCGC'y
Cuts at: 1678 2236 4821 1678
Size: 558 2585 2531
Fragments arranged by size:
2585 2531 558
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2652 2684 4315 4347 4389
Size: 32 135 32 1080 32 1631 32 42
Cuts at: 4389 4421 1373
Size: 32 2626
Fragments arranged by size:
2626 1631 1080 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2566 2671 3018 3264 669
Size: 988 281 628 105 347 246 3079
Fragments arranged by size:
3079 988 628 347 281 246 105
HindIII A'AGCT_T
Cuts at: 748 2078 748
Size: 1330 4344
HpaI GTT'AAC
Cuts at: 2566 3264 2566
Size: 698 4976
KpnI G_GTAC'C
Cuts at: 2999 2999
Size: 5674
MfeI C'AATT_G
Cuts at: 3273 3273
Size: 5674
MluI A'CGCG_T
Cuts at: 5550 5550
Size: 5674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2460 4788 4972 1305
Size: 1155 2328 184 2007
Fragments arranged by size:
2328 2007 1155 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2679 10
Size: 54 1425 343 847 3005
Fragments arranged by size:
3005 1425 847 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2376 3632 3991 4150 518
Size: 648 1210 1256 359 159 2042
Fragments arranged by size:
2042 1256 1210 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2427 3090 3767 4915 5160 1606
Size: 821 663 677 1148 245 2120
Fragments arranged by size:
2120 1148 821 677 663 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2674 513
Size: 552 939 670 3513
Fragments arranged by size:
3513 939 670 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5674
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5674
NotI GC'GGCC_GC
Cuts at: 3121 3121
Size: 5674
NspI r_CATG'y
Cuts at: 1132 1229 2322 2442 2913 3032 4577 1132
Size: 97 1093 120 471 119 1545 2229
Fragments arranged by size:
2229 1545 1093 471 120 119 97
PacI TTA_AT'TAA
Cuts at: 2088 2088
Size: 5674
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5674
PciI A'CATG_T
Cuts at: 4573 4573
Size: 5674
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2686 1108
Size: 1578 4096
PmeI GTTT'AAAC
Cuts at: 2978 2978
Size: 5674
PmlI CAC'GTG
Cuts at: 2335 2335
Size: 5674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2373 2405 2946 2978 3743 3775 5256 5288 2373
Size: 32 541 32 765 32 1481 32 2759
Fragments arranged by size:
2759 1481 765 541 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5674
PsiI TTA'TAA
Cuts at: 3244 3244
Size: 5674
PstI C_TGCA'G
Cuts at: 830 2224 2426 2710 3026 830
Size: 1394 202 284 316 3478
Fragments arranged by size:
3478 1394 316 284 202
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5674
SalI G'TCGA_C
Cuts at: 1936 2669 3016 1936
Size: 733 347 4594
Fragments arranged by size:
4594 733 347
SbfI CC_TGCA'GG
Cuts at: 3026 3026
Size: 5674
ScaI AGT'ACT
Cuts at: 3922 5634 3922
Size: 1712 3962
SfcI C'TryA_G
Cuts at: 826 1045 2220 2422 2706 3022 4157 4838 5029
Size: 219 1175 202 284 316 1135 681 191
Cuts at: 5029 826
Size: 1471
Fragments arranged by size:
1471 1175 1135 681 316 284 219 202
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5674
SmaI CCC'GGG
Cuts at: 1872 3001 1872
Size: 1129 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3778 4679 4941 820
Size: 197 926 1835 901 262 1553
Fragments arranged by size:
1835 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3465 493
Size: 2972 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5674
SphI G_CATG'C
Cuts at: 1132 2322 3032 1132
Size: 1190 710 3774
Fragments arranged by size:
3774 1190 710
SspI AAT'ATT
Cuts at: 5 52 3598 5
Size: 47 3546 2081
Fragments arranged by size:
3546 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5674
StyI C'CwwG_G
Cuts at: 513 1065 2004 2323 2469 2674 3113 513
Size: 552 939 319 146 205 439 3074
Fragments arranged by size:
3074 939 552 439 319 205 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2891 3701 3718 3871 4056 4501 325
Size: 1255 1311 810 17 153 185 445 1498
Fragments arranged by size:
1498 1311 1255 810 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3920 5632 96
Size: 274 80 33 51 1443 1943 1712 138
Fragments arranged by size:
1943 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2319 2351 2826 2858 5634 5666 2319
Size: 32 475 32 2776 32 2327
Fragments arranged by size:
2776 2327 475 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5674
XbaI T'CTAG_A
Cuts at: 2650 3010 2650
Size: 360 5314
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2353 2476 2353
Size: 123 5551
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5674
XmaI C'CCGG_G
Cuts at: 1870 2999 1870
Size: 1129 4545
XmnI GAAnn'nnTTC
Cuts at: 3803 3803
Size: 5674
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3482 276
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI