ASB8 ORF clone pFN21AE3974

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Halotag ORF Clone pFN21AE3974

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Clone info.

Species

human

Clone Name

pFN21AE3974 Sequence (with vector) Restriction Map

Product ID

FHC24703

ORF Sequence

DNA sequence (864 bp)
Protein sequence (288 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3974

Symbol

ASB8

Alias : PP14212

Description

ankyrin repeat and SOCS box containing 8

Original Clone ID

cp93974

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 864 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 288 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8761.1 3.3e-90 100.0 ASB8 CCDS81686.1 1.4e-21 100.0 ASB8 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR002110 56 83 PF00023 Ankyrin repeat IPR002110 86 115 PF00023 Ankyrin repeat IPR002110 119 148 PF00023 Ankyrin repeat IPR002110 151 179 PF00023 Ankyrin repeat IPR001496 247 286 PF07525 SOCS protein HMMSmart IPR002110 52 81 SM00248 Ankyrin repeat IPR002110 85 113 SM00248 Ankyrin repeat IPR002110 117 146 SM00248 Ankyrin repeat IPR002110 150 179 SM00248 Ankyrin repeat IPR001496 247 287 SM00969 SOCS protein ProfileScan IPR002110 52 84 PS50088 Ankyrin repeat IPR020683 52 188 PS50297 Ankyrin repeat-containing domain IPR002110 85 116 PS50088 Ankyrin repeat IPR002110 117 149 PS50088 Ankyrin repeat IPR002110 150 182 PS50088 Ankyrin repeat IPR001496 235 288 PS50225 SOCS protein
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001306226 288 615053 100.0 100.0 NP_077000 288 615053 100.0 100.0 NP_001306225 288 615053 100.0 100.0 NP_078977 278 615055 32.7 86.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3974 Download

>KIEE3974  864 bp
ATGAGTTCCAGTATGTGGTATATTATGCAGAGCATTCAGAGCAAATACTCTCTCTCCGAG
CGCTTAATCCGAACAATTGCTGCCATCCGTTCCTTCCCACATGATAATGTAGAGGACCTC
ATCAGAGGGGGAGCAGATGTGAACTGCACTCATGGCACACTGAAGCCCTTGCACTGTGCC
TGTATGGTGTCAGATGCTGACTGTGTGGAGTTACTTCTGGAAAAAGGAGCCGAGGTGAAT
GCCCTGGATGGGTATAACCGAACAGCCCTCCACTATGCAGCAGAGAAAGATGAGGCTTGT
GTGGAGGTCCTATTGGAGTATGGTGCAAACCCCAATGCTTTGGATGGCAACAGAGATACC
CCACTTCACTGGGCAGCCTTTAAGAACAATGCTGAGTGTGTGCGGGCTCTCCTAGAGAGC
GGGGCCTCTGTCAATGCCCTGGATTACAACAATGATACACCGCTCAGCTGGGCTGCCATG
AAGGGAAATCTTGAGAGTGTCAGCATCCTTCTGGATTATGGCGCAGAGGTCAGAGTCATC
AACCTAATAGGCCAGACACCCATCTCCCGCCTGGTGGCTCTGCTAGTCAGGGGACTTGGA
ACAGAGAAAGAGGACTCTTGCTTTGAGCTCCTCCACAGAGCTGTTGGACACTTTGAATTG
AGGAAAAATGGCACCATGCCACGAGAGGTGGCCAGAGACCCGCAGCTATGTGAAAAACTG
ACTGTTCTGTGCTCAGCTCCAGGAACTCTAAAAACACTCGCTCGCTATGCCGTGCGCCGT
AGCCTGGGACTCCAGTATCTCCCCGATGCAGTGAAGGGCCTTCCACTGCCAGCTTCTTTG
AAGGAATACCTGTTACTTTTAGAA

ORF Protein

Cloned ORF protein sequence for pFN21AE3974 Download

>KIEE3974  288 aa
MSSSMWYIMQSIQSKYSLSERLIRTIAAIRSFPHDNVEDLIRGGADVNCTHGTLKPLHCA
CMVSDADCVELLLEKGAEVNALDGYNRTALHYAAEKDEACVEVLLEYGANPNALDGNRDT
PLHWAAFKNNAECVRALLESGASVNALDYNNDTPLSWAAMKGNLESVSILLDYGAEVRVI
NLIGQTPISRLVALLVRGLGTEKEDSCFELLHRAVGHFELRKNGTMPREVARDPQLCEKL
TVLCSAPGTLKTLARYAVRRSLGLQYLPDAVKGLPLPASLKEYLLLLE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3974 Download

>pFN21AE3974  5569 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGTTCCAGTATGTGGTATATTATGCAGAGCAT
TCAGAGCAAATACTCTCTCTCCGAGCGCTTAATCCGAACAATTGCTGCCATCCGTTCCTT
CCCACATGATAATGTAGAGGACCTCATCAGAGGGGGAGCAGATGTGAACTGCACTCATGG
CACACTGAAGCCCTTGCACTGTGCCTGTATGGTGTCAGATGCTGACTGTGTGGAGTTACT
TCTGGAAAAAGGAGCCGAGGTGAATGCCCTGGATGGGTATAACCGAACAGCCCTCCACTA
TGCAGCAGAGAAAGATGAGGCTTGTGTGGAGGTCCTATTGGAGTATGGTGCAAACCCCAA
TGCTTTGGATGGCAACAGAGATACCCCACTTCACTGGGCAGCCTTTAAGAACAATGCTGA
GTGTGTGCGGGCTCTCCTAGAGAGCGGGGCCTCTGTCAATGCCCTGGATTACAACAATGA
TACACCGCTCAGCTGGGCTGCCATGAAGGGAAATCTTGAGAGTGTCAGCATCCTTCTGGA
TTATGGCGCAGAGGTCAGAGTCATCAACCTAATAGGCCAGACACCCATCTCCCGCCTGGT
GGCTCTGCTAGTCAGGGGACTTGGAACAGAGAAAGAGGACTCTTGCTTTGAGCTCCTCCA
CAGAGCTGTTGGACACTTTGAATTGAGGAAAAATGGCACCATGCCACGAGAGGTGGCCAG
AGACCCGCAGCTATGTGAAAAACTGACTGTTCTGTGCTCAGCTCCAGGAACTCTAAAAAC
ACTCGCTCGCTATGCCGTGCGCCGTAGCCTGGGACTCCAGTATCTCCCCGATGCAGTGAA
GGGCCTTCCACTGCCAGCTTCTTTGAAGGAATACCTGTTACTTTTAGAAGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT