(Circular) (Six-base) MAPSORT of: pFN21AE3974.seq Check: 7839 from: 1 to: 5569 pFN21AE3974 5569 bp With 182 enzymes: SgfI * March 28, 2012 11:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3379 278 Size: 53 83 186 936 1843 2468 Fragments arranged by size: 2468 1843 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2912 1937 Size: 975 4594 Acc65I G'GTAC_C Cuts at: 2890 2890 Size: 5569 AclI AA'CG_TT Cuts at: 1563 3696 4069 1563 Size: 2133 373 3063 Fragments arranged by size: 3063 2133 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2186 3632 5016 5284 1632 Size: 554 1446 1384 268 1917 Fragments arranged by size: 1917 1446 1384 554 268 AfeI AGC'GCT Cuts at: 2066 2066 Size: 5569 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5372 AflIII A'CryG_T Cuts at: 1203 4468 5445 1203 Size: 3265 977 1327 Fragments arranged by size: 3265 1327 977 AgeI A'CCGG_T Cuts at: 4400 4400 Size: 5569 AhdI GACnn_n'nnGTC Cuts at: 4298 4298 Size: 5569 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5569 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5569 AlwNI CAG_nnn'CTG Cuts at: 2201 3312 4884 2201 Size: 1111 1572 2886 Fragments arranged by size: 2886 1572 1111 ApaLI G'TGCA_C Cuts at: 3626 4782 3626 Size: 1156 4413 ApoI r'AATT_y Cuts at: 1493 2878 3104 3256 5365 1493 Size: 1385 226 152 2109 1697 Fragments arranged by size: 2109 1697 1385 226 152 AseI AT'TA_AT Cuts at: 160 1032 4123 160 Size: 872 3091 1606 Fragments arranged by size: 3091 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5569 AvaI C'yCGr_G Cuts at: 1870 1943 2894 1870 Size: 73 951 4545 Fragments arranged by size: 4545 951 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2783 2816 2783 Size: 33 5536 BamHI G'GATC_C Cuts at: 2899 3266 2899 Size: 367 5202 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2675 2890 4345 618 Size: 325 217 627 888 215 1455 1842 Fragments arranged by size: 1842 1455 888 627 325 217 215 BanII G_rGCy'C Cuts at: 1391 2413 2634 2888 1391 Size: 1022 221 254 4072 Fragments arranged by size: 4072 1022 254 221 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4598 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2225 2259 3760 3794 1903 Size: 34 288 34 1501 34 3678 Fragments arranged by size: 3678 1501 288 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3461 4671 3461 Size: 1210 4359 BclI T'GATC_A Cuts at: 1548 1548 Size: 5569 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4180 136 Size: 107 122 71 631 605 2508 1525 Fragments arranged by size: 2508 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5564 5564 Size: 5569 BlpI GC'TnA_GC Cuts at: 2468 2737 2986 2468 Size: 269 249 5051 Fragments arranged by size: 5051 269 249 Bme1580I G_kGCm'C Cuts at: 1790 3630 4786 1790 Size: 1840 1156 2573 Fragments arranged by size: 2573 1840 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5569 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2383 3300 4258 Size: 416 53 83 399 24 966 917 958 Cuts at: 4258 442 Size: 1753 Fragments arranged by size: 1753 966 958 917 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5569 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2727 2780 4229 1239 Size: 1488 53 1449 2579 Fragments arranged by size: 2579 1488 1449 53 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2516 3694 4559 4857 2516 Size: 1178 865 298 3228 Fragments arranged by size: 3228 1178 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2694 4232 882 Size: 617 1195 1538 2219 Fragments arranged by size: 2219 1538 1195 617 BsaAI yAC'GTr Cuts at: 493 1204 3360 493 Size: 711 2156 2702 Fragments arranged by size: 2702 2156 711 BsaBI GATnn'nnATC Cuts at: 5563 5563 Size: 5569 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3376 3758 4393 275 Size: 53 83 186 936 1843 382 635 1451 Fragments arranged by size: 1843 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4002 4400 4674 4821 5232 4002 Size: 398 274 147 411 4339 Fragments arranged by size: 4339 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2042 2072 2127 2157 2223 Size: 30 165 30 639 30 55 30 66 Cuts at: 2223 2253 1178 Size: 30 4494 Fragments arranged by size: 4494 639 165 66 55 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2624 1186 Size: 1438 4131 BseYI C'CCAG_C Cuts at: 2472 4772 5268 2472 Size: 2300 496 2773 Fragments arranged by size: 2773 2300 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2134 1163 Size: 573 398 4598 Fragments arranged by size: 4598 573 398 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3019 3780 4808 1540 Size: 275 186 1018 761 1028 2301 Fragments arranged by size: 2301 1028 1018 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2634 2738 3630 3715 4786 2634 Size: 104 892 85 1071 3417 Fragments arranged by size: 3417 1071 892 104 85 BsmI GAATG_Cn' Cuts at: 2037 2248 3080 3173 2037 Size: 211 832 93 4433 Fragments arranged by size: 4433 832 211 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2413 2634 2738 2888 3630 3715 4786 Size: 399 623 221 104 150 742 85 1071 Cuts at: 4786 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 623 399 221 150 104 85 BspEI T'CCGG_A Cuts at: 5232 5232 Size: 5569 BspHI T'CATG_A Cuts at: 3458 5188 3458 Size: 1730 3839 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2924 844 Size: 2080 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2424 2467 3456 1394 Size: 429 601 43 989 3507 Fragments arranged by size: 3507 989 601 429 43 BsrDI GCAATG_nn' Cuts at: 65 1248 4064 4238 65 Size: 1183 2816 174 1396 Fragments arranged by size: 2816 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4213 4400 1700 Size: 110 2403 187 2869 Fragments arranged by size: 2869 2403 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5569 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5569 BssSI C'ACGA_G Cuts at: 2685 3629 4641 2685 Size: 944 1012 3613 Fragments arranged by size: 3613 1012 944 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5569 BstBI TT'CG_AA Cuts at: 4373 4373 Size: 5569 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5569 BstXI CCAn_nnnn'nTGG Cuts at: 2344 2373 2344 Size: 29 5540 BstYI r'GATC_y Cuts at: 1972 2899 3266 3651 3668 5120 5206 5218 5564 Size: 927 367 385 17 1452 86 12 346 Cuts at: 5564 1972 Size: 1977 Fragments arranged by size: 1977 1452 927 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5017 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4830 Fragments arranged by size: 4830 683 56 BtsI GCAGTG_nn' Cuts at: 2820 2828 3085 3879 3899 2820 Size: 8 257 794 20 4490 Fragments arranged by size: 4490 794 257 20 8 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5569 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5534 DraI TTT'AAA Cuts at: 2873 2873 Size: 5569 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4576 809 Size: 507 3260 1802 Fragments arranged by size: 3260 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2694 3016 3905 Size: 54 1425 50 275 18 864 322 889 Cuts at: 3905 5534 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 864 322 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3016 1537 Size: 275 1204 4090 Fragments arranged by size: 4090 1204 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3499 1353 Size: 2146 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4156 4530 4676 387 Size: 1064 138 2567 374 146 1280 Fragments arranged by size: 2567 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2632 2632 Size: 5569 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2186 2727 2780 3632 4229 5016 5284 Size: 393 554 541 53 852 597 787 268 Cuts at: 5284 1239 Size: 1524 Fragments arranged by size: 1524 852 787 597 554 541 393 268 53 EcoO109I rG'GnC_Cy Cuts at: 2119 2311 2427 2821 2119 Size: 192 116 394 4867 Fragments arranged by size: 4867 394 192 116 EcoRI G'AATT_C Cuts at: 2878 3256 2878 Size: 378 5191 FspI TGC'GCA Cuts at: 4075 4075 Size: 5569 HaeII r_GCGC'y Cuts at: 1678 2068 4716 1678 Size: 390 2648 2531 Fragments arranged by size: 2648 2531 390 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4210 4242 4284 4316 1373 Size: 32 135 32 2638 32 42 32 2626 Fragments arranged by size: 2638 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2913 3159 669 Size: 988 281 975 246 3079 Fragments arranged by size: 3079 988 975 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5569 HpaI GTT'AAC Cuts at: 3159 3159 Size: 5569 KpnI G_GTAC'C Cuts at: 2894 2894 Size: 5569 MfeI C'AATT_G Cuts at: 2079 3168 2079 Size: 1089 4480 MluI A'CGCG_T Cuts at: 5445 5445 Size: 5569 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2629 4683 4867 1305 Size: 1324 2054 184 2007 Fragments arranged by size: 2054 2007 1324 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2696 10 Size: 54 1425 343 864 2883 Fragments arranged by size: 2883 1425 864 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2109 3527 3886 4045 518 Size: 648 943 1418 359 159 2042 Fragments arranged by size: 2042 1418 943 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2472 2985 3662 4810 5055 1606 Size: 866 513 677 1148 245 2120 Fragments arranged by size: 2120 1148 866 677 513 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5017 NdeI CA'TA_TG Cuts at: 387 387 Size: 5569 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5569 NotI GC'GGCC_GC Cuts at: 3016 3016 Size: 5569 NspI r_CATG'y Cuts at: 1132 1229 2927 4472 1132 Size: 97 1698 1545 2229 Fragments arranged by size: 2229 1698 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5569 PciI A'CATG_T Cuts at: 4468 4468 Size: 5569 PflMI CCAn_nnn'nTGG Cuts at: 1108 2019 1108 Size: 911 4658 PfoI T'CCnGG_A Cuts at: 2743 2743 Size: 5569 PmeI GTTT'AAAC Cuts at: 2873 2873 Size: 5569 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3638 3670 5151 5183 3638 Size: 32 1481 32 4024 Fragments arranged by size: 4024 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2119 2311 2119 Size: 192 5377 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5569 PsiI TTA'TAA Cuts at: 3139 3139 Size: 5569 PstI C_TGCA'G Cuts at: 830 2921 830 Size: 2091 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5569 PvuII CAG'CTG Cuts at: 2472 2472 Size: 5569 SacI G_AGCT'C Cuts at: 2634 2634 Size: 5569 SalI G'TCGA_C Cuts at: 1936 2911 1936 Size: 975 4594 SbfI CC_TGCA'GG Cuts at: 2921 2921 Size: 5569 ScaI AGT'ACT Cuts at: 3817 5529 3817 Size: 1712 3857 SfcI C'TryA_G Cuts at: 826 1045 2917 4052 4733 4924 826 Size: 219 1872 1135 681 191 1471 Fragments arranged by size: 1872 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5569 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5569 SmaI CCC'GGG Cuts at: 1872 2896 1872 Size: 1024 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2495 3673 4574 4836 820 Size: 197 926 552 1178 901 262 1553 Fragments arranged by size: 1553 1178 926 901 552 262 197 SnaBI TAC'GTA Cuts at: 493 3360 493 Size: 2867 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5569 SphI G_CATG'C Cuts at: 1132 2927 1132 Size: 1795 3774 SspI AAT'ATT Cuts at: 5 52 3493 5 Size: 47 3441 2081 Fragments arranged by size: 3441 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5569 StyI C'CwwG_G Cuts at: 513 1065 3008 513 Size: 552 1943 3074 Fragments arranged by size: 3074 1943 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2578 3596 3613 3766 3951 4396 325 Size: 1255 998 1018 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1018 998 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3815 5527 96 Size: 274 80 33 51 1443 1838 1712 138 Fragments arranged by size: 1838 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2127 2159 2223 2255 5529 5561 2127 Size: 32 64 32 3274 32 2135 Fragments arranged by size: 3274 2135 64 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5569 XbaI T'CTAG_A Cuts at: 2905 2905 Size: 5569 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2572 2645 2572 Size: 73 5496 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5569 XmaI C'CCGG_G Cuts at: 1870 2894 1870 Size: 1024 4545 XmnI GAAnn'nnTTC Cuts at: 3698 3698 Size: 5569 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3377 276 Size: 53 83 186 936 1843 2468 Fragments arranged by size: 2468 1843 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI