Restriction map for
pFN21AE3974
(Linear) (Six-base) MAPSORT of: KIEE3974.seq Check: 8228 from: 1 to: 864
KIEE3974.orf
With 182 enzymes: SgfI *
March 22, 2012 17:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 864
Size: 181 683
AfeI AGC'GCT
Cuts at: 0 61 864
Size: 61 803
AlwNI CAG_nnn'CTG
Cuts at: 0 196 864
Size: 196 668
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 778 811 864
Size: 778 33 53
Fragments arranged by size:
778 53 33
BanI G'GyrC_C
Cuts at: 0 670 864
Size: 670 194
BanII G_rGCy'C
Cuts at: 0 408 629 864
Size: 408 221 235
Fragments arranged by size:
408 235 221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 220 254 864
Size: 220 34 610
Fragments arranged by size:
610 220 34
BlpI GC'TnA_GC
Cuts at: 0 463 732 864
Size: 463 269 132
Fragments arranged by size:
463 269 132
BmrI ACTGGGnnnn_n'
Cuts at: 0 378 864
Size: 378 486
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 722 775 864
Size: 722 53 89
Fragments arranged by size:
722 89 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 864
Size: 511 353
BsaI GGTCTCn'nnnn_
Cuts at: 0 689 864
Size: 689 175
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 37 67 122 152 218 248 864
Size: 37 30 55 30 66 30 616
Fragments arranged by size:
616 66 55 37 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 619 864
Size: 619 245
BseYI C'CCAG_C
Cuts at: 0 467 864
Size: 467 397
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 864
Size: 129 735
BsiHKAI G_wGCw'C
Cuts at: 0 629 733 864
Size: 629 104 131
Fragments arranged by size:
629 131 104
BsmI GAATG_Cn'
Cuts at: 0 32 243 864
Size: 32 211 621
Fragments arranged by size:
621 211 32
Bsp1286I G_dGCh'C
Cuts at: 0 408 629 733 864
Size: 408 221 104 131
Fragments arranged by size:
408 221 131 104
BsrBI CCG'CTC
Cuts at: 0 419 462 864
Size: 419 43 402
Fragments arranged by size:
419 402 43
BssSI C'ACGA_G
Cuts at: 0 680 864
Size: 680 184
BstXI CCAn_nnnn'nTGG
Cuts at: 0 339 368 864
Size: 339 29 496
Fragments arranged by size:
496 339 29
BtsI GCAGTG_nn'
Cuts at: 0 815 823 864
Size: 815 8 41
Fragments arranged by size:
815 41 8
EaeI y'GGCC_r
Cuts at: 0 689 864
Size: 689 175
EcoICRI GAG'CTC
Cuts at: 0 627 864
Size: 627 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 722 775 864
Size: 181 541 53 89
Fragments arranged by size:
541 181 89 53
EcoO109I rG'GnC_Cy
Cuts at: 0 114 306 422 816 864
Size: 114 192 116 394 48
Fragments arranged by size:
394 192 116 114 48
HaeII r_GCGC'y
Cuts at: 0 63 864
Size: 63 801
MfeI C'AATT_G
Cuts at: 0 74 864
Size: 74 790
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 624 864
Size: 624 240
MscI TGG'CCA
Cuts at: 0 691 864
Size: 691 173
MslI CAynn'nnrTG
Cuts at: 0 104 864
Size: 104 760
MspA1I CmG'CkG
Cuts at: 0 467 864
Size: 467 397
PflMI CCAn_nnn'nTGG
Cuts at: 0 14 864
Size: 14 850
PfoI T'CCnGG_A
Cuts at: 0 738 864
Size: 738 126
PpuMI rG'GwC_Cy
Cuts at: 0 114 306 864
Size: 114 192 558
Fragments arranged by size:
558 192 114
PvuII CAG'CTG
Cuts at: 0 467 864
Size: 467 397
SacI G_AGCT'C
Cuts at: 0 629 864
Size: 629 235
SmlI C'TyrA_G
Cuts at: 0 490 864
Size: 490 374
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 573 864
Size: 573 291
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 122 154 218 250 864
Size: 122 32 64 32 614
Fragments arranged by size:
614 122 64 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 567 640 864
Size: 567 73 224
Fragments arranged by size:
567 224 73
Enzymes that do cut:
AcuI AfeI AlwNI BaeI BanI BanII BcgI BlpI
BmrI BpmI BpuEI BsaI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BsrBI BssSI BstXI BtsI EaeI
EcoICRI Eco57MI EcoO109I HaeII MfeI MmeI MscI MslI
MspA1I PflMI PfoI PpuMI PvuII SacI SmlI TaqII
TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BamHI BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII Bme1580I BmgBI BmtI
BplI Bpu10I BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EagI EarI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KasI KpnI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TatI Tth111I XbaI XhoI XmaI
XmnI ZraI