################################## # NO CUT ENZYMES ( 175 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DpnII DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeII MamI MboI MboII MflI MluI MroI MspI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp45I Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 25 enzymes) # ################################### AlwNI 191 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 59 AGCGCT AGC^GCT BalI 689 TGGCCA TGG^CCA BanI 670 GGCACC G^G[CT][GA]CC BcgI 231 CGAGGTGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaI 695 GAGACC GGTCTC(1/5) BseRI 628 CTCCTC GAGGAG(10/8) BsgI 144 CTGCAC GTGCAG(16/14) BsmAI 695 GAGAC GTCTC(1/5) BssSI 680 CACGAG CACGAG(-5/-1) EaeI 689 TGGCCA [CT]^GGCC[GA] Eco47III 59 AGCGCT AGC^GCT HaeII 59 AGCGCT [GA]GCGC^[CT] HphI 234 GGTGA GGTGA(8/7) MfeI 74 CAATTG C^AATTG MluNI 689 TGGCCA TGG^CCA MscI 689 TGGCCA TGG^CCA MslI 100 CATGATAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 465 CAGCTG C[AC]G^C[GT]G MunI 74 CAATTG C^AATTG PflMI 8 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 465 CAGCTG CAG^CTG SacI 625 GAGCTC GAGCT^C SmlI 490 CTTGAG C^T[CT][GA]AG Van91I 8 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AspHI 625 GAGCTC G[AT]GC[AT]^C 729 GTGCTC AvaII 114 GGACC G^G[AT]CC 306 GGTCC BanII 404 GGGCTC G[GA]GC[CT]^C 625 GAGCTC BfaI 412 CTAG C^TAG 583 CTAG BlpI 462 GCTCAGC GC^T[ACGT]AGC 731 GCTCAGC BpmI 737 CTCCAG CTGGAG(16/14) 790 CTCCAG Bpu1102I 462 GCTCAGC GC^T[ACGT]AGC 731 GCTCAGC BsiHKAI 625 GAGCTC G[AT]GC[AT]^C 729 GTGCTC BsmFI 591 GGGAC GGGAC(10/14) 786 GGGAC BsmI 237 GAATGC GAATGC(1/-1) 32 GCATTC BsrBI 460 CCGCTC CCGCTC(-3/-3) 417 GAGCGG BstXI 332 CCAATGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 361 CCACTTCACTGG CelII 462 GCTCAGC GC^T[ACGT]AGC 731 GCTCAGC MaeI 412 CTAG C^TAG 583 CTAG MaeIII 210 GTTAC ^GT[ACGT]AC 852 GTTAC MseI 64 TTAA T^TAA 380 TTAA PpuMI 113 AGGACCT [GA]G^G[AT]CC[CT] 305 AGGTCCT Tru9I 64 TTAA T^TAA 380 TTAA Tsp509I 75 AATT ^AATT 656 AATT XcmI 560 CCATCTCCCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 633 CCACAGAGCTGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AluI BbvI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HhaI HinP1I HinfI ItaI MnlI MvaI MwoI NlaIII NlaIV PleI Sau96I ScrFI SfaNI TseI TspRI