HMG20B ORF clone pFN21AE3678

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Halotag ORF Clone pFN21AE3678

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Clone info.

Species

human

Clone Name

pFN21AE3678 Sequence (with vector) Restriction Map

Product ID

FHC30780

ORF Sequence

DNA sequence (951 bp)
Protein sequence (317 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3678

Symbol

HMG20B

Alias : BRAF25, BRAF35, HMGX2, HMGXB2, PP7706, SMARCE1r, SOXL, pp8857

Description

high mobility group 20B

Original Clone ID

oc93678

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 951 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 317 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS45919.1 1.7e-88 100.0 HMG20B CCDS10295.1 1.5e-38 48.4 HMG20A The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000135 85 103 PR00886 High mobility group IPR000135 103 122 PR00886 High mobility group IPR000135 122 142 PR00886 High mobility group HMMPfam IPR000910 70 138 PF00505 High mobility group HMMSmart IPR000910 69 139 SM00398 High mobility group ProfileScan IPR000910 70 138 PS50118 High mobility group
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_006330 317 605535 100.0 100.0 XP_016881633 316 605535 99.7 100.0 XP_011519460 347 605534 48.4 98.4 NP_060670 347 605534 48.4 98.4 NP_001291433 347 605534 48.4 98.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3678 Download

>KIEE3678  951 bp
ATGTCCCACGGCCCCAAGCAGCCCGGCGCGGCCGCCGCGCCGGCGGGCGGCAAGGCTCCG
GGCCAGCATGGGGGCTTCGTGGTGACTGTCAAGCAAGAGCGCGGCGAGGGTCCACGCGCG
GGCGAGAAGGGGTCCCACGAGGAGGAGCCGGTGAAGAAACGCGGCTGGCCCAAGGGCAAG
AAGCGGAAGAAGATTCTGCCGAATGGGCCCAAGGCACCGGTCACGGGCTACGTGCGCTTC
CTGAACGAGCGGCGCGAGCAGATCCGCACGCGCCACCCGGATCTGCCCTTTCCCGAGATC
ACCAAGATGCTGGGCGCCGAGTGGAGCAAGCTGCAGCCAACGGAAAAGCAGCGGTACCTG
GATGAGGCCGAGAGAGAGAAGCAGCAGTACATGAAGGAGCTGCGGGCGTACCAGCAGTCT
GAAGCCTATAAGATGTGCACGGAGAAGATCCAGGAGAAGAAGATCAAGAAAGAAGACTCG
AGCTCTGGGCTCATGAACACTCTCCTGAATGGACACAAGGGTGGGGACTGCGATGGCTTC
TCCACCTTCGATGTTCCCATCTTCACTGAAGAGTTCTTGGACCAAAACAAAGCGCGTGAG
GCGGAGCTTCGGCGCTTGCGGAAGATGAATGTGGCCTTCGAGGAGCAGAACGCGGTACTG
CAGAGGCACACGCAGAGCATGAGCAGCGCGCGCGAGCGTCTGGAGCAGGAGCTGGCGCTG
GAGGAGCGGAGGACGCTGGCGCTGCAGCAGCAGCTCCAGGCCGTGCGCCAGGCGCTCACC
GCCAGCTTCGCCTCACTGCCGGTGCCGGGCACGGGCGAAACGCCCACGCTGGGCACTCTG
GACTTCTACATGGCCCGGCTTCACGGAGCCATCGAGCGCGACCCCGCCCAGCACGAGAAG
CTCATCGTCCGCATCAAGGAAATCCTGGCCCAGGTCGCCAGCGAGCACCTG

ORF Protein

Cloned ORF protein sequence for pFN21AE3678 Download

>KIEE3678  317 aa
MSHGPKQPGAAAAPAGGKAPGQHGGFVVTVKQERGEGPRAGEKGSHEEEPVKKRGWPKGK
KRKKILPNGPKAPVTGYVRFLNERREQIRTRHPDLPFPEITKMLGAEWSKLQPTEKQRYL
DEAEREKQQYMKELRAYQQSEAYKMCTEKIQEKKIKKEDSSSGLMNTLLNGHKGGDCDGF
STFDVPIFTEEFLDQNKAREAELRRLRKMNVAFEEQNAVLQRHTQSMSSARERLEQELAL
EERRTLALQQQLQAVRQALTASFASLPVPGTGETPTLGTLDFYMARLHGAIERDPAQHEK
LIVRIKEILAQVASEHL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3678 Download

>pFN21AE3678  5656 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCCCACGGCCCCAAGCAGCCCGGCGCGGCCGC
CGCGCCGGCGGGCGGCAAGGCTCCGGGCCAGCATGGGGGCTTCGTGGTGACTGTCAAGCA
AGAGCGCGGCGAGGGTCCACGCGCGGGCGAGAAGGGGTCCCACGAGGAGGAGCCGGTGAA
GAAACGCGGCTGGCCCAAGGGCAAGAAGCGGAAGAAGATTCTGCCGAATGGGCCCAAGGC
ACCGGTCACGGGCTACGTGCGCTTCCTGAACGAGCGGCGCGAGCAGATCCGCACGCGCCA
CCCGGATCTGCCCTTTCCCGAGATCACCAAGATGCTGGGCGCCGAGTGGAGCAAGCTGCA
GCCAACGGAAAAGCAGCGGTACCTGGATGAGGCCGAGAGAGAGAAGCAGCAGTACATGAA
GGAGCTGCGGGCGTACCAGCAGTCTGAAGCCTATAAGATGTGCACGGAGAAGATCCAGGA
GAAGAAGATCAAGAAAGAAGACTCGAGCTCTGGGCTCATGAACACTCTCCTGAATGGACA
CAAGGGTGGGGACTGCGATGGCTTCTCCACCTTCGATGTTCCCATCTTCACTGAAGAGTT
CTTGGACCAAAACAAAGCGCGTGAGGCGGAGCTTCGGCGCTTGCGGAAGATGAATGTGGC
CTTCGAGGAGCAGAACGCGGTACTGCAGAGGCACACGCAGAGCATGAGCAGCGCGCGCGA
GCGTCTGGAGCAGGAGCTGGCGCTGGAGGAGCGGAGGACGCTGGCGCTGCAGCAGCAGCT
CCAGGCCGTGCGCCAGGCGCTCACCGCCAGCTTCGCCTCACTGCCGGTGCCGGGCACGGG
CGAAACGCCCACGCTGGGCACTCTGGACTTCTACATGGCCCGGCTTCACGGAGCCATCGA
GCGCGACCCCGCCCAGCACGAGAAGCTCATCGTCCGCATCAAGGAAATCCTGGCCCAGGT
CGCCAGCGAGCACCTGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT