################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BseAI BsgI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrGI BsrI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MunI NcoI NdeI NheI NruI NsiI NspI NspV PacI PflFI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 353 GGTACC G^GTACC AcyI 313 GGCGCC G[GA]^CG[CT]C AgeI 216 ACCGGT A^CCGGT Alw44I 435 GTGCAC G^TGCAC AlwNI 706 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 205 GGGCCC GGGCC^C ApaLI 435 GTGCAC G^TGCAC Asp718I 353 GGTACC G^GTACC BbeI 313 GGCGCC GGCGC^C BbsI 472 GAAGAC GAAGAC(2/6) BpuAI 472 GAAGAC GAAGAC(2/6) BsaAI 229 TACGTG [CT]AC^GT[GA] BsaHI 313 GGCGCC G[GA]^CG[CT]C BsaWI 216 ACCGGT [AT]^CCGG[AT] BsiEI 29 CGGCCG CG[GA][CT]^CG BspHI 491 TCATGA T^CATGA BstF5I 360 GGATG GGATG(2/0) BstXI 302 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 130 GGGTCCC [GA]G^G[ACGT]CC[CT] DsaI 6 CCACGG C^C[GA][CT]GG EaeI 29 CGGCCG [CT]^GGCC[GA] EagI 29 CGGCCG C^GGCCG EarI 568 GAAGAG CTCTTC(1/4) EclXI 29 CGGCCG C^GGCCG Eco52I 29 CGGCCG C^GGCCG EcoO109I 130 GGGTCCC [GA]G^G[ACGT]CC[CT] FokI 360 GGATG GGATG(9/13) Hin1I 313 GGCGCC G[GA]^CG[CT]C KasI 313 GGCGCC G^GCGCC KpnI 353 GGTACC GGTAC^C Ksp632I 568 GAAGAG CTCTTC(1/4) MaeII 230 ACGT A^CGT MspA1I 349 CAGCGG C[AC]G^C[GT]G NaeI 39 GCCGGC GCC^GGC NarI 313 GGCGCC GG^CGCC NgoMIV 39 GCCGGC G^CCGGC NotI 28 GCGGCCGC GC^GGCCGC PaeR7I 477 CTCGAG C^TCGAG PinAI 216 ACCGGT A^CCGGT PleI 475 GACTC GAGTC(4/5) PpuMI 130 GGGTCCC [GA]G^G[AT]CC[CT] RcaI 491 TCATGA T^CATGA SacI 480 GAGCTC GAGCT^C SfoI 313 GGCGCC GGC^GCC SgrAI 38 CGCCGGCG C[GA]^CCGG[CT]G SmlI 477 CTCGAG C^T[CT][GA]AG TfiI 192 GATTC G^A[AT]TC XhoI 477 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AvaI 292 CCCGAG C^[CT]CG[GA]G 477 CTCGAG BglI 34 GCCGCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 198 GCCGAATGGGC BsoBI 292 CCCGAG C^[CT]CG[GA]G 477 CTCGAG BsrBI 247 GAGCGG CCGCTC(-3/-3) 724 GAGCGG BssHII 686 GCGCGC G^CGCGC 688 GCGCGC BssSI 136 CACGAG CACGAG(-5/-1) 892 CACGAG EcoNI 357 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 924 CCTGGCCCAGG EcoT14I 170 CCAAGG C^C[AT][AT]GG 209 CCAAGG HgaI 732 GACGC GACGC(5/10) 696 GCGTC HinfI 192 GATTC G^A[ACGT]TC 475 GACTC MaeIII 82 GTGAC ^GT[ACGT]AC 220 GTCAC MslI 300 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 514 CACAAGGGTG SfaNI 306 GATGC GCATC(5/9) 911 GCATC StyI 170 CCAAGG C^C[AT][AT]GG 209 CCAAGG Tsp45I 82 GTGAC ^GT[GC]AC 220 GTCAC TspRI 564 CACTG CA[GC]TG[ACGT][ACGT]^ 794 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrFI BssKI BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DpnII EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MboI MboII MflI MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfcI TaqI TseI TthHB8I XhoII