(Circular) (Six-base) MAPSORT of: pFN21AE3678.seq Check: 4661 from: 1 to: 5656 pFN21AE3678 5656 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3466 278 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2999 1937 Size: 1062 4594 Acc65I G'GTAC_C Cuts at: 2358 2977 2358 Size: 619 5037 AclI AA'CG_TT Cuts at: 1563 3783 4156 1563 Size: 2220 373 3063 Fragments arranged by size: 3063 2220 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2445 2592 3719 5103 5371 1632 Size: 813 147 1127 1384 268 1917 Fragments arranged by size: 1917 1384 1127 813 268 147 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5459 AflIII A'CryG_T Cuts at: 1203 4555 5532 1203 Size: 3352 977 1327 Fragments arranged by size: 3352 1327 977 AgeI A'CCGG_T Cuts at: 2221 4487 2221 Size: 2266 3390 AhdI GACnn_n'nnGTC Cuts at: 4385 4385 Size: 5656 AleI CACnn'nnGTG Cuts at: 1166 2523 1166 Size: 1357 4299 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5656 AlwNI CAG_nnn'CTG Cuts at: 2716 3399 4971 2716 Size: 683 1572 3401 Fragments arranged by size: 3401 1572 683 ApaI G_GGCC'C Cuts at: 2214 2214 Size: 5656 ApaLI G'TGCA_C Cuts at: 2440 3713 4869 2440 Size: 1273 1156 3227 Fragments arranged by size: 3227 1273 1156 ApoI r'AATT_y Cuts at: 1493 2965 3191 3343 5452 1493 Size: 1472 226 152 2109 1697 Fragments arranged by size: 2109 1697 1472 226 152 AseI AT'TA_AT Cuts at: 160 1032 4210 160 Size: 872 3178 1606 Fragments arranged by size: 3178 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 AvaI C'yCGr_G Cuts at: 1870 1943 2297 2482 2981 1870 Size: 73 354 185 499 4545 Fragments arranged by size: 4545 499 354 185 73 BamHI G'GATC_C Cuts at: 2986 3353 2986 Size: 367 5289 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2218 2318 2358 2806 2977 Size: 325 217 627 431 100 40 448 171 Cuts at: 2977 4432 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 448 431 325 217 171 100 40 BanII G_rGCy'C Cuts at: 1391 2214 2489 2496 2975 1391 Size: 823 275 7 479 4072 Fragments arranged by size: 4072 823 479 275 7 BbeI G_GCGC'C Cuts at: 2322 2322 Size: 5656 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2484 928 Size: 971 585 4100 Fragments arranged by size: 4100 971 585 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3847 3881 1903 Size: 34 1910 34 3678 Fragments arranged by size: 3678 1910 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3548 4758 3548 Size: 1210 4446 BclI T'GATC_A Cuts at: 1548 1548 Size: 5656 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2045 2209 4267 Size: 107 122 71 631 605 373 164 2058 Cuts at: 4267 136 Size: 1525 Fragments arranged by size: 2058 1525 631 605 373 164 122 107 71 BglII A'GATC_T Cuts at: 5651 5651 Size: 5656 BlpI GC'TnA_GC Cuts at: 3073 3073 Size: 5656 Bme1580I G_kGCm'C Cuts at: 1790 2214 2444 2816 2840 3717 4873 1790 Size: 424 230 372 24 877 1156 2573 Fragments arranged by size: 2573 1156 877 424 372 230 24 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5656 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3387 4345 442 Size: 416 53 83 399 24 1970 958 1753 Fragments arranged by size: 1970 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5656 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2726 2744 4316 1239 Size: 1487 18 1572 2579 Fragments arranged by size: 2579 1572 1487 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3781 4646 4944 3781 Size: 865 298 4493 Fragments arranged by size: 4493 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4319 882 Size: 617 2820 2219 Fragments arranged by size: 2820 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2236 3447 493 Size: 711 1032 1211 2702 Fragments arranged by size: 2702 1211 1032 711 BsaBI GATnn'nnATC Cuts at: 5650 5650 Size: 5656 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2319 3463 3845 4480 Size: 53 83 186 936 786 1144 382 635 Cuts at: 4480 275 Size: 1451 Fragments arranged by size: 1451 1144 936 786 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2221 4089 4487 4761 4908 5319 2221 Size: 1868 398 274 147 411 2558 Fragments arranged by size: 2558 1868 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5431 Fragments arranged by size: 5431 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2159 2162 2660 2741 1186 Size: 973 3 498 81 4101 Fragments arranged by size: 4101 973 498 81 3 BseYI C'CCAG_C Cuts at: 2314 2833 2892 4859 5355 2314 Size: 519 59 1967 496 2615 Fragments arranged by size: 2615 1967 519 496 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5083 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2037 3106 3867 4895 1540 Size: 275 186 36 1069 761 1028 2301 Fragments arranged by size: 2301 1069 1028 761 275 186 36 BsiHKAI G_wGCw'C Cuts at: 2444 2489 2952 3717 3802 4873 2444 Size: 45 463 765 85 1071 3227 Fragments arranged by size: 3227 1071 765 463 85 45 BsmI GAATG_Cn' Cuts at: 3167 3260 3167 Size: 93 5563 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2214 2444 2489 2496 2816 2840 2952 Size: 399 424 230 45 7 320 24 112 Cuts at: 2952 2975 3717 3802 4873 1391 Size: 23 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 424 399 320 230 112 85 45 24 23 7 BspEI T'CCGG_A Cuts at: 5319 5319 Size: 5656 BspHI T'CATG_A Cuts at: 2496 3545 5275 2496 Size: 1049 1730 2877 Fragments arranged by size: 2877 1730 1049 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3011 844 Size: 2167 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2254 2731 3543 1394 Size: 429 431 477 812 3507 Fragments arranged by size: 3507 812 477 431 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4151 4325 65 Size: 1183 2903 174 1396 Fragments arranged by size: 2903 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2044 2152 2221 2803 4300 4487 1700 Size: 110 234 108 69 582 1497 187 2869 Fragments arranged by size: 2869 1497 582 234 187 110 108 69 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5656 BssHII G'CGCG_C Cuts at: 1925 2691 2693 1925 Size: 766 2 4888 Fragments arranged by size: 4888 766 2 BssSI C'ACGA_G Cuts at: 2141 2897 3716 4728 2141 Size: 756 819 1012 3069 Fragments arranged by size: 3069 1012 819 756 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5656 BstBI TT'CG_AA Cuts at: 4460 4460 Size: 5656 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5656 BstXI CCAn_nnnn'nTGG Cuts at: 2314 2314 Size: 5656 BstYI r'GATC_y Cuts at: 1972 2265 2284 2451 2986 3353 3738 3755 5207 Size: 293 19 167 535 367 385 17 1452 Cuts at: 5207 5293 5305 5651 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 535 385 367 346 293 167 86 19 17 12 BtgI C'CryG_G Cuts at: 513 1065 2011 513 Size: 552 946 4158 Fragments arranged by size: 4158 946 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2550 487 Size: 683 56 1324 3593 Fragments arranged by size: 3593 1324 683 56 BtsI GCAGTG_nn' Cuts at: 2798 3172 3966 3986 2798 Size: 374 794 20 4468 Fragments arranged by size: 4468 794 374 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5656 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5621 DraI TTT'AAA Cuts at: 2960 2960 Size: 5656 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4663 809 Size: 507 3347 1802 Fragments arranged by size: 3347 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 3103 3992 Size: 54 1425 50 275 18 204 1069 889 Cuts at: 3992 5621 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1069 889 275 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2034 3103 1537 Size: 275 222 1069 4090 Fragments arranged by size: 4090 1069 275 222 EarI CTCTTCn'nnn_ Cuts at: 1353 2568 3586 1353 Size: 1215 1018 3423 Fragments arranged by size: 3423 1215 1018 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2621 4243 4617 4763 387 Size: 1064 138 1032 1622 374 146 1280 Fragments arranged by size: 1622 1280 1064 1032 374 146 138 EcoICRI GAG'CTC Cuts at: 2487 2487 Size: 5656 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2445 2592 2726 2744 3719 4316 5103 Size: 393 813 147 134 18 975 597 787 Cuts at: 5103 5371 1239 Size: 268 1524 Fragments arranged by size: 1524 975 813 787 597 393 268 147 134 18 EcoNI CCTnn'n_nnAGG Cuts at: 2366 2933 2366 Size: 567 5089 EcoO109I rG'GnC_Cy Cuts at: 2136 2136 Size: 5656 EcoRI G'AATT_C Cuts at: 2965 3343 2965 Size: 378 5278 FspI TGC'GCA Cuts at: 4162 4162 Size: 5656 HaeII r_GCGC'y Cuts at: 1678 2322 2620 2723 2747 2780 4803 1678 Size: 644 298 103 24 33 2023 2531 Fragments arranged by size: 2531 2023 644 298 103 33 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2120 2152 2471 2503 2528 Size: 32 135 32 548 32 319 32 25 Cuts at: 2528 2560 4297 4329 4371 4403 1373 Size: 32 1737 32 42 32 2626 Fragments arranged by size: 2626 1737 548 319 135 42 32 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 3000 3246 669 Size: 988 281 1062 246 3079 Fragments arranged by size: 3079 1062 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5656 HpaI GTT'AAC Cuts at: 3246 3246 Size: 5656 KasI G'GCGC_C Cuts at: 2318 2318 Size: 5656 KpnI G_GTAC'C Cuts at: 2362 2981 2362 Size: 619 5037 MfeI C'AATT_G Cuts at: 3255 3255 Size: 5656 MluI A'CGCG_T Cuts at: 5532 5532 Size: 5656 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4770 4954 1305 Size: 3465 184 2007 Fragments arranged by size: 3465 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3834 Fragments arranged by size: 3834 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2309 2523 3614 3973 4132 518 Size: 648 1143 214 1091 359 159 2042 Fragments arranged by size: 2042 1143 1091 648 359 214 159 MspA1I CmG'CkG Cuts at: 1606 2356 3072 3749 4897 5142 1606 Size: 750 716 677 1148 245 2120 Fragments arranged by size: 2120 1148 750 716 677 245 NaeI GCC'GGC Cuts at: 2046 2046 Size: 5656 NarI GG'CG_CC Cuts at: 2319 2319 Size: 5656 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5104 NdeI CA'TA_TG Cuts at: 387 387 Size: 5656 NgoMIV G'CCGG_C Cuts at: 2044 2044 Size: 5656 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5656 NotI GC'GGCC_GC Cuts at: 2034 3103 2034 Size: 1069 4587 NspI r_CATG'y Cuts at: 1132 1229 3014 4559 1132 Size: 97 1785 1545 2229 Fragments arranged by size: 2229 1785 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5656 PciI A'CATG_T Cuts at: 4555 4555 Size: 5656 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5656 PfoI T'CCnGG_A Cuts at: 2454 2454 Size: 5656 PmeI GTTT'AAAC Cuts at: 2960 2960 Size: 5656 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3725 3757 5238 5270 3725 Size: 32 1481 32 4111 Fragments arranged by size: 4111 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2136 2136 Size: 5656 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5656 PsiI TTA'TAA Cuts at: 3226 3226 Size: 5656 PspOMI G'GGCC_C Cuts at: 2210 2210 Size: 5656 PspXI vC'TCGA_Gb Cuts at: 2482 2482 Size: 5656 PstI C_TGCA'G Cuts at: 830 2340 2667 2751 3008 830 Size: 1510 327 84 257 3478 Fragments arranged by size: 3478 1510 327 257 84 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5656 SacI G_AGCT'C Cuts at: 2489 2489 Size: 5656 SalI G'TCGA_C Cuts at: 1936 2998 1936 Size: 1062 4594 SanDI GG'GwC_CC Cuts at: 2136 2136 Size: 5656 SbfI CC_TGCA'GG Cuts at: 3008 3008 Size: 5656 ScaI AGT'ACT Cuts at: 3904 5616 3904 Size: 1712 3944 SexAI A'CCwGG_T Cuts at: 2952 2952 Size: 5656 SfcI C'TryA_G Cuts at: 826 1045 2336 2663 2747 3004 4139 4820 5011 Size: 219 1291 327 84 257 1135 681 191 Cuts at: 5011 826 Size: 1471 Fragments arranged by size: 1471 1291 1135 681 327 257 219 191 84 SfoI GGC'GCC Cuts at: 2320 2320 Size: 5656 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 SgrAI Cr'CCGG_yG Cuts at: 1700 2044 1700 Size: 344 5312 SmaI CCC'GGG Cuts at: 1872 2983 1872 Size: 1111 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2482 3760 4661 4923 820 Size: 197 926 539 1278 901 262 1553 Fragments arranged by size: 1553 1278 926 901 539 262 197 SnaBI TAC'GTA Cuts at: 493 3447 493 Size: 2954 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5656 SphI G_CATG'C Cuts at: 1132 3014 1132 Size: 1882 3774 SspI AAT'ATT Cuts at: 5 52 3580 5 Size: 47 3528 2081 Fragments arranged by size: 3528 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5656 StyI C'CwwG_G Cuts at: 513 1065 2175 2214 3095 513 Size: 552 1110 39 881 3074 Fragments arranged by size: 3074 1110 881 552 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3683 3700 3853 4038 4483 325 Size: 1255 2103 17 153 185 445 1498 Fragments arranged by size: 2103 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2391 3902 5614 Size: 274 80 33 51 1443 414 1511 1712 Cuts at: 5614 96 Size: 138 Fragments arranged by size: 1712 1511 1443 414 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5616 5648 5616 Size: 32 5624 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5656 XbaI T'CTAG_A Cuts at: 2992 2992 Size: 5656 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2950 2950 Size: 5656 XhoI C'TCGA_G Cuts at: 1943 2482 1943 Size: 539 5117 XmaI C'CCGG_G Cuts at: 1870 2981 1870 Size: 1111 4545 XmnI GAAnn'nnTTC Cuts at: 3785 3785 Size: 5656 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3464 276 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PsrI PvuII RsrII SacII SapI SfiI SrfI SwaI