SCG2 ORF clone pFN21AE3258

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE3258

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Clone info.

Species

human

Clone Name

pFN21AE3258 Sequence (with vector) Restriction Map

Product ID

FHC12720

ORF Sequence

DNA sequence (1851 bp)
Protein sequence (617 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3258

Symbol

SCG2

Alias : CHGC, EM66, SN, SgII

Description

secretogranin II

Original Clone ID

cp93258

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1851 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 617 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2457.1 3.6e-193 99.5 SCG2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001990 1 614 PF01271 Chromogranin/secretogranin
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 7 HWLGAALSLIPLIFLISGAEAA 28 PRIMARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_003460 617 118930 99.5 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3258 Download

>KIEE3258  1851 bp
ATGGCTGAAGCAAAGACCCACTGGCTTGGAGCAGCCCTGTCTCTTATCCCTTTAATTTTC
CTCATCTCTGGGGCTGAAGCAGCTTCATTTCAGAGAAACCAGCTGCTTCAGAAAGAACCA
GACCTCAGGTTGGAAAATGTCCAAAAGTTTCCCAGTCCTGAAATGATCAGGGCTTTGGAG
TACATAGAAAACCTCCGACAACAAGCTCATAAGGAAGAAAGCAGCCCAGATTATAATCCC
TACCAAGGTGTCTCTGTCCCCCTTCAGCAAAAAGAAAATGGCGATGAAAGCCACTTGCCC
GAGAGGGATTCACTGAGTGAAGAAGACTGGATGAGAATAATACTCGAAGCTTTGAGACAG
GCTGAAAATGAGCCTCAGTCTGCACCAAAAGAAAATAAGCCCTATGCCTTGAATTCAGAA
AAGAACTTTCCAATGGACATGAGTGATGATTATGAGACACAGCAGTGGCCAGAAAGAAAG
CTTAAGCACATGCAATTCCCTCCTATGTATGAAGAGAATTCCAGGGATAACCCCTTTAAA
CGCACAAATGAAATAGTGGAGGAACAATATACTCCTCAAAGCCTTGCTACATTGGAATCT
GTCTTCCAAGAGCTGGGGAAACTGACAGGACCAAACAACCAGAAACGTGAGAGGATGGAT
GAGGAGCAAAAACTTTATACGGATGATGAAGATGATATCTACAAGGCTAATAACATTGCC
TATGAAGATGTGGTCGGGGGAGAAGACTGGAACCCAGTAGAGGAGAAAATAGAGAGTCAA
ACCCAGGAAGAGGTGAGAGACAGCAAAGAGAATATAGAAAAAAATGAACAAATCAATGAT
GAGATGAAACGCTCAGGGCAGCTTGGCATCCAGGAAGAAGGTCTTCGGAAAGAGAGTAAA
GACCAACTCTCAGATGATGTCTCCAAAGTAATTGCCTATTTGAAAAGGTTAGTAAATGCT
GCAGGAAGTGGGAGGTTACAGAATGGGCAAAATGGGGAAAGGGCCACCAGGCTTTTTGAG
AAACCTCTTGATTCTCAGTCTATTTATCAGCTGATTGAAATCTCAAGGAATTTACAGATA
CCCCCAGAAGACTTAATTGAGATGCTCAAAACTGGGGAGAAGCCGAATGGATCAGTGGAA
CCGGAGCGGGAGCTTGACCTTCCTGTTGACCTAGATGACATCTCAGAGGCTGACTTAGAC
CATCCAGACCTGTTCCAAAATAGGATGCTCTCCAAGAGTGGCTACCCTAAAACACCTGGT
GGTGCTGGGACTGAGGCCCTACCAGACGGGCTCAGTGTTGAGGATATTTTAAATCTTTTA
GGGATGGAGAGTGCAGCAAATCAGAAAACGTCGTATTTTCCCAATCCATATAACCAGGAG
AAAGTTCTGCCAAGGCTCCCTTATGGTGCTGGAAGATCTAGATCGAACCAGCTTCCCAAA
GCTGCCTGGATTCCACATGTTGAAAACAGACAGATGGCATATGAAAACCTGAACGACAAG
GATCAAGAATTAGGTGAGTACTTGGCCAGGATGCTAGTTAAATACCCTGAGATCATTAAT
TCAAACCAAGTGAAGCGAGTTCCTGGTCAAGGCTCATCTGAAGGTGACCTGCAGGAAGAG
GAACAAATTGAGCAGGCCATCAAAGAGCATTTGAATCAAGGCAGCTCTCAGGAGACTGAC
AAGCTGGCCCCGGTGAGCAAAAGGTTCCCTGTGGGGCCCCCGAAGAATGATGATACCCCA
AATAGGCAGTACTGGGATGAAGATCTGTTAATGAAAGTGCTGGAATACCTCAACCAAGAA
AAGGCAGAAAAGGGAAGGGAGCATATTGCTAAGAGAGCAATGGAAAATATG

ORF Protein

Cloned ORF protein sequence for pFN21AE3258 Download

>KIEE3258  617 aa
MAEAKTHWLGAALSLIPLIFLISGAEAASFQRNQLLQKEPDLRLENVQKFPSPEMIRALE
YIENLRQQAHKEESSPDYNPYQGVSVPLQQKENGDESHLPERDSLSEEDWMRIILEALRQ
AENEPQSAPKENKPYALNSEKNFPMDMSDDYETQQWPERKLKHMQFPPMYEENSRDNPFK
RTNEIVEEQYTPQSLATLESVFQELGKLTGPNNQKRERMDEEQKLYTDDEDDIYKANNIA
YEDVVGGEDWNPVEEKIESQTQEEVRDSKENIEKNEQINDEMKRSGQLGIQEEGLRKESK
DQLSDDVSKVIAYLKRLVNAAGSGRLQNGQNGERATRLFEKPLDSQSIYQLIEISRNLQI
PPEDLIEMLKTGEKPNGSVEPERELDLPVDLDDISEADLDHPDLFQNRMLSKSGYPKTPG
GAGTEALPDGLSVEDILNLLGMESAANQKTSYFPNPYNQEKVLPRLPYGAGRSRSNQLPK
AAWIPHVENRQMAYENLNDKDQELGEYLARMLVKYPEIINSNQVKRVPGQGSSEGDLQEE
EQIEQAIKEHLNQGSSQETDKLAPVSKRFPVGPPKNDDTPNRQYWDEDLLMKVLEYLNQE
KAEKGREHIAKRAMENM

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3258 Download

>pFN21AE3258  6556 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCTGAAGCAAAGACCCACTGGCTTGGAGCAGC
CCTGTCTCTTATCCCTTTAATTTTCCTCATCTCTGGGGCTGAAGCAGCTTCATTTCAGAG
AAACCAGCTGCTTCAGAAAGAACCAGACCTCAGGTTGGAAAATGTCCAAAAGTTTCCCAG
TCCTGAAATGATCAGGGCTTTGGAGTACATAGAAAACCTCCGACAACAAGCTCATAAGGA
AGAAAGCAGCCCAGATTATAATCCCTACCAAGGTGTCTCTGTCCCCCTTCAGCAAAAAGA
AAATGGCGATGAAAGCCACTTGCCCGAGAGGGATTCACTGAGTGAAGAAGACTGGATGAG
AATAATACTCGAAGCTTTGAGACAGGCTGAAAATGAGCCTCAGTCTGCACCAAAAGAAAA
TAAGCCCTATGCCTTGAATTCAGAAAAGAACTTTCCAATGGACATGAGTGATGATTATGA
GACACAGCAGTGGCCAGAAAGAAAGCTTAAGCACATGCAATTCCCTCCTATGTATGAAGA
GAATTCCAGGGATAACCCCTTTAAACGCACAAATGAAATAGTGGAGGAACAATATACTCC
TCAAAGCCTTGCTACATTGGAATCTGTCTTCCAAGAGCTGGGGAAACTGACAGGACCAAA
CAACCAGAAACGTGAGAGGATGGATGAGGAGCAAAAACTTTATACGGATGATGAAGATGA
TATCTACAAGGCTAATAACATTGCCTATGAAGATGTGGTCGGGGGAGAAGACTGGAACCC
AGTAGAGGAGAAAATAGAGAGTCAAACCCAGGAAGAGGTGAGAGACAGCAAAGAGAATAT
AGAAAAAAATGAACAAATCAATGATGAGATGAAACGCTCAGGGCAGCTTGGCATCCAGGA
AGAAGGTCTTCGGAAAGAGAGTAAAGACCAACTCTCAGATGATGTCTCCAAAGTAATTGC
CTATTTGAAAAGGTTAGTAAATGCTGCAGGAAGTGGGAGGTTACAGAATGGGCAAAATGG
GGAAAGGGCCACCAGGCTTTTTGAGAAACCTCTTGATTCTCAGTCTATTTATCAGCTGAT
TGAAATCTCAAGGAATTTACAGATACCCCCAGAAGACTTAATTGAGATGCTCAAAACTGG
GGAGAAGCCGAATGGATCAGTGGAACCGGAGCGGGAGCTTGACCTTCCTGTTGACCTAGA
TGACATCTCAGAGGCTGACTTAGACCATCCAGACCTGTTCCAAAATAGGATGCTCTCCAA
GAGTGGCTACCCTAAAACACCTGGTGGTGCTGGGACTGAGGCCCTACCAGACGGGCTCAG
TGTTGAGGATATTTTAAATCTTTTAGGGATGGAGAGTGCAGCAAATCAGAAAACGTCGTA
TTTTCCCAATCCATATAACCAGGAGAAAGTTCTGCCAAGGCTCCCTTATGGTGCTGGAAG
ATCTAGATCGAACCAGCTTCCCAAAGCTGCCTGGATTCCACATGTTGAAAACAGACAGAT
GGCATATGAAAACCTGAACGACAAGGATCAAGAATTAGGTGAGTACTTGGCCAGGATGCT
AGTTAAATACCCTGAGATCATTAATTCAAACCAAGTGAAGCGAGTTCCTGGTCAAGGCTC
ATCTGAAGGTGACCTGCAGGAAGAGGAACAAATTGAGCAGGCCATCAAAGAGCATTTGAA
TCAAGGCAGCTCTCAGGAGACTGACAAGCTGGCCCCGGTGAGCAAAAGGTTCCCTGTGGG
GCCCCCGAAGAATGATGATACCCCAAATAGGCAGTACTGGGATGAAGATCTGTTAATGAA
AGTGCTGGAATACCTCAACCAAGAAAAGGCAGAAAAGGGAAGGGAGCATATTGCTAAGAG
AGCAATGGAAAATATGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT