Restriction map for
pFN21AE3258
(Circular) (Six-base) MAPSORT of: pFN21AE3258.seq Check: 8607 from: 1 to: 6556
pFN21AE3258 6556 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4366 278
Size: 53 83 186 936 2830 2468
Fragments arranged by size:
2830 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3899 1937
Size: 1962 4594
Acc65I G'GTAC_C
Cuts at: 3877 3877
Size: 6556
AclI AA'CG_TT
Cuts at: 1563 4683 5056 1563
Size: 3120 373 3063
Fragments arranged by size:
3120 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2031 2096 2100 2252 3624 4619 6003 6271
Size: 399 65 4 152 1372 995 1384 268
Cuts at: 6271 1632
Size: 1917
Fragments arranged by size:
1917 1384 1372 995 399 268 152 65
4
AflII C'TTAA_G
Cuts at: 820 1017 2486 820
Size: 197 1469 4890
Fragments arranged by size:
4890 1469 197
AflIII A'CryG_T
Cuts at: 1203 3460 5455 6432 1203
Size: 2257 1995 977 1327
Fragments arranged by size:
2257 1995 1327 977
AgeI A'CCGG_T
Cuts at: 5387 5387
Size: 6556
AhdI GACnn_n'nnGTC
Cuts at: 5285 5285
Size: 6556
AleI CACnn'nnGTG
Cuts at: 1166 2467 1166
Size: 1301 5255
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6556
AlwNI CAG_nnn'CTG
Cuts at: 3194 4299 5871 3194
Size: 1105 1572 3879
Fragments arranged by size:
3879 1572 1105
ApaI G_GGCC'C
Cuts at: 3723 3723
Size: 6556
ApaLI G'TGCA_C
Cuts at: 4613 5769 4613
Size: 1156 5400
ApoI r'AATT_y
Cuts at: 1493 2416 2521 3073 3865 4091 4243 6352 1493
Size: 923 105 552 792 226 152 2109 1697
Fragments arranged by size:
2109 1697 923 792 552 226 152 105
AseI AT'TA_AT
Cuts at: 160 1032 3561 5110 160
Size: 872 2529 1549 1606
Fragments arranged by size:
2529 1606 1549 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6556
AvaI C'yCGr_G
Cuts at: 1870 1943 2303 3881 1870
Size: 73 360 1578 4545
Fragments arranged by size:
4545 1578 360 73
BamHI G'GATC_C
Cuts at: 3886 4253 3886
Size: 367 6189
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3877 5332 618
Size: 325 217 627 2090 1455 1842
Fragments arranged by size:
2090 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3297 3723 3875 1391
Size: 1906 426 152 4072
Fragments arranged by size:
4072 1906 426 152
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2334 2599 2754 2879 3099 928
Size: 971 435 265 155 125 220 4385
Fragments arranged by size:
4385 971 435 265 220 155 125
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4747 4781 1903
Size: 34 2810 34 3678
Fragments arranged by size:
3678 2810 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4448 5658 4448
Size: 1210 5346
BclI T'GATC_A
Cuts at: 1548 2169 1548
Size: 621 5935
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5167 136
Size: 107 122 71 631 605 3495 1525
Fragments arranged by size:
3495 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3419 3766 6551 3419
Size: 347 2785 3424
Fragments arranged by size:
3424 2785 347
BlpI GC'TnA_GC
Cuts at: 3973 3973
Size: 6556
Bme1580I G_kGCm'C
Cuts at: 1790 3723 4617 5773 1790
Size: 1933 894 1156 2573
Fragments arranged by size:
2573 1933 1156 894
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6556
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2151 2753 3126
Size: 416 53 83 399 24 734 602 373
Cuts at: 3126 3766 4287 5245 442
Size: 640 521 958 1753
Fragments arranged by size:
1753 958 734 640 602 521 416 399
373 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6556
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5216 1239
Size: 3977 2579
Bpu10I CC'TnA_GC
Cuts at: 2857 2857
Size: 6556
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3052 4681 5546 5844 3052
Size: 1629 865 298 3764
Fragments arranged by size:
3764 1629 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5219 882
Size: 617 3720 2219
Fragments arranged by size:
3720 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4347 493
Size: 711 3143 2702
Fragments arranged by size:
3143 2702 711
BsaBI GATnn'nnATC
Cuts at: 2238 3062 3424 3765 6550 2238
Size: 824 362 341 2785 2244
Fragments arranged by size:
2785 2244 824 362 341
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4363 4745 5380 275
Size: 53 83 186 936 2830 382 635 1451
Fragments arranged by size:
2830 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3145 4989 5387 5661 5808 6219 3145
Size: 1844 398 274 147 411 3482
Fragments arranged by size:
3482 1844 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6331
Fragments arranged by size:
6331 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2568 2681 2780 1186
Size: 1382 113 99 4962
Fragments arranged by size:
4962 1382 113 99
BseYI C'CCAG_C
Cuts at: 2617 3269 5759 6255 2617
Size: 652 2490 496 2918
Fragments arranged by size:
2918 2490 652 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2370 3357 1163
Size: 573 634 987 4362
Fragments arranged by size:
4362 987 634 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4006 4767 5795 1540
Size: 275 186 2005 761 1028 2301
Fragments arranged by size:
2301 2005 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4617 4702 5773 4617
Size: 85 1071 5400
Fragments arranged by size:
5400 1071 85
BsmI GAATG_Cn'
Cuts at: 4067 4160 4067
Size: 93 6463
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3297 3723 3875 4617 4702 5773 1391
Size: 399 1507 426 152 742 85 1071 2174
Fragments arranged by size:
2174 1507 1071 742 426 399 152 85
BspEI T'CCGG_A
Cuts at: 6219 6219
Size: 6556
BspHI T'CATG_A
Cuts at: 4445 6175 4445
Size: 1730 4826
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3621 3911 844
Size: 2777 290 3489
Fragments arranged by size:
3489 2777 290
BsrBI CCG'CTC
Cuts at: 1394 1823 3151 4443 1394
Size: 429 1328 1292 3507
Fragments arranged by size:
3507 1328 1292 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2718 3849 5051 5225 65
Size: 1183 1470 1131 1202 174 1396
Fragments arranged by size:
1470 1396 1202 1183 1131 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5200 5387 1700
Size: 110 3390 187 2869
Fragments arranged by size:
3390 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6556
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6556
BssSI C'ACGA_G
Cuts at: 4616 5628 4616
Size: 1012 5544
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6556
BstBI TT'CG_AA
Cuts at: 5360 5360
Size: 6556
BstEII G'GTnAC_C
Cuts at: 1184 3608 1184
Size: 2424 4132
BstXI CCAn_nnnn'nTGG
Cuts at: 2030 2030
Size: 6556
BstYI r'GATC_y
Cuts at: 1972 3419 3766 3886 4253 4638 4655 6107 6193
Size: 1447 347 120 367 385 17 1452 86
Cuts at: 6193 6205 6551 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1447 385 367 347 346 120
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2129 2129
Size: 6556
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5065
Fragments arranged by size:
5065 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2301 487
Size: 683 56 1075 4742
Fragments arranged by size:
4742 1075 683 56
BtsI GCAGTG_nn'
Cuts at: 2474 4072 4866 4886 2474
Size: 1598 794 20 4144
Fragments arranged by size:
4144 1598 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6556
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6521
DraI TTT'AAA
Cuts at: 2542 3315 3860 2542
Size: 773 545 5238
Fragments arranged by size:
5238 773 545
DraIII CAC_nnn'GTG
Cuts at: 2321 3263 2321
Size: 942 5614
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5563 809
Size: 507 4247 1802
Fragments arranged by size:
4247 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2471 3528 4003
Size: 54 1425 50 275 18 641 1057 475
Cuts at: 4003 4892 6521 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1057 889 641 475 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4003 1537
Size: 275 2191 4090
Fragments arranged by size:
4090 2191 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2511 2787 3615 4486 1353
Size: 1158 276 828 871 3423
Fragments arranged by size:
3423 1158 871 828 276
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5143 5517 5663 387
Size: 1064 138 3554 374 146 1280
Fragments arranged by size:
3554 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2031 2096 2100 2252 3624 4619 5216
Size: 393 399 65 4 152 1372 995 597
Cuts at: 5216 6003 6271 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1372 995 787 597 399 393 268
152 65 4
EcoNI CCTnn'n_nnAGG
Cuts at: 3591 3591
Size: 6556
EcoO109I rG'GnC_Cy
Cuts at: 3280 3719 3720 3280
Size: 439 1 6116
Fragments arranged by size:
6116 439 1
EcoRI G'AATT_C
Cuts at: 2416 2521 3865 4243 2416
Size: 105 1344 378 4729
Fragments arranged by size:
4729 1344 378 105
EcoRV GAT'ATC
Cuts at: 2701 2701
Size: 6556
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2903 3420 2903
Size: 517 6039
FspI TGC'GCA
Cuts at: 5062 5062
Size: 6556
HaeII r_GCGC'y
Cuts at: 1678 5703 1678
Size: 4025 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2366 2398 2897 2909 2929
Size: 32 135 32 794 32 499 12 20
Cuts at: 2929 2941 3026 3058 3170 3202 5197 5229 5271
Size: 12 85 32 112 32 1995 32 42
Cuts at: 5271 5303 1373
Size: 32 2626
Fragments arranged by size:
2626 1995 794 499 135 112 85 42
32 32 32 32 32 32 32 20
12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3172 3900 4146 669
Size: 988 281 1234 728 246 3079
Fragments arranged by size:
3079 1234 988 728 281 246
HindIII A'AGCT_T
Cuts at: 748 2352 2483 748
Size: 1604 131 4821
Fragments arranged by size:
4821 1604 131
HpaI GTT'AAC
Cuts at: 4146 4146
Size: 6556
KpnI G_GTAC'C
Cuts at: 3881 3881
Size: 6556
MfeI C'AATT_G
Cuts at: 4155 4155
Size: 6556
MluI A'CGCG_T
Cuts at: 6432 6432
Size: 6556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2115 2224 5670 5854 1305
Size: 810 109 3446 184 2007
Fragments arranged by size:
3446 2007 810 184 109
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2473 3530 10
Size: 54 1425 343 641 1057 3036
Fragments arranged by size:
3036 1425 1057 641 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2467 4514 4873 5032 518
Size: 648 1301 2047 359 159 2042
Fragments arranged by size:
2047 2042 1301 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2107 3055 3972 4649 5797 6042 1606
Size: 501 948 917 677 1148 245 2120
Fragments arranged by size:
2120 1148 948 917 677 501 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5065
Fragments arranged by size:
5065 939 552
NdeI CA'TA_TG
Cuts at: 387 3484 387
Size: 3097 3459
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6556
NotI GC'GGCC_GC
Cuts at: 4003 4003
Size: 6556
NspI r_CATG'y
Cuts at: 1132 1229 2497 3464 3914 5459 1132
Size: 97 1268 967 450 1545 2229
Fragments arranged by size:
2229 1545 1268 967 450 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6556
PciI A'CATG_T
Cuts at: 3460 5455 3460
Size: 1995 4561
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2617 1108
Size: 1509 5047
PfoI T'CCnGG_A
Cuts at: 2874 2874
Size: 6556
PmeI GTTT'AAAC
Cuts at: 3860 3860
Size: 6556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2112 2144 4625 4657 6138 6170 2112
Size: 32 2481 32 1481 32 2498
Fragments arranged by size:
2498 2481 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6556
PsiI TTA'TAA
Cuts at: 2238 4126 2238
Size: 1888 4668
PspOMI G'GGCC_C
Cuts at: 3719 3719
Size: 6556
PstI C_TGCA'G
Cuts at: 830 2968 3618 3908 830
Size: 2138 650 290 3478
Fragments arranged by size:
3478 2138 650 290
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6556
PvuII CAG'CTG
Cuts at: 2107 3055 2107
Size: 948 5608
SalI G'TCGA_C
Cuts at: 1936 3898 1936
Size: 1962 4594
SbfI CC_TGCA'GG
Cuts at: 3618 3908 3618
Size: 290 6266
ScaI AGT'ACT
Cuts at: 3524 3755 4804 6516 3524
Size: 231 1049 1712 3564
Fragments arranged by size:
3564 1712 1049 231
SexAI A'CCwGG_T
Cuts at: 3259 3259
Size: 6556
SfcI C'TryA_G
Cuts at: 826 1045 2964 3614 3904 5039 5720 5911 826
Size: 219 1919 650 290 1135 681 191 1471
Fragments arranged by size:
1919 1471 1135 681 650 290 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6556
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6556
SmaI CCC'GGG
Cuts at: 1872 3883 1872
Size: 2011 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2486 3067 4660 5561 5823 820
Size: 197 926 543 581 1593 901 262 1553
Fragments arranged by size:
1593 1553 926 901 581 543 262 197
SnaBI TAC'GTA
Cuts at: 493 4347 493
Size: 3854 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6556
SphI G_CATG'C
Cuts at: 1132 3914 1132
Size: 2782 3774
SspI AAT'ATT
Cuts at: 5 52 4480 5
Size: 47 4428 2081
Fragments arranged by size:
4428 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6556
StyI C'CwwG_G
Cuts at: 513 1065 2004 2248 3395 3995 513
Size: 552 939 244 1147 600 3074
Fragments arranged by size:
3074 1147 939 600 552 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4583 4600 4753 4938 5383 325
Size: 1255 3003 17 153 185 445 1498
Fragments arranged by size:
3003 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 3522 3753
Size: 274 80 33 51 1443 207 1338 231
Cuts at: 3753 4802 6514 96
Size: 1049 1712 138
Fragments arranged by size:
1712 1443 1338 1049 274 231 207 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3319 3351 6516 6548 3319
Size: 32 3165 32 3327
Fragments arranged by size:
3327 3165 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6556
XbaI T'CTAG_A
Cuts at: 3422 3892 3422
Size: 470 6086
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6556
XmaI C'CCGG_G
Cuts at: 1870 3881 1870
Size: 2011 4545
XmnI GAAnn'nnTTC
Cuts at: 2886 4685 2886
Size: 1799 4757
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4364 276
Size: 53 83 186 936 2830 2468
Fragments arranged by size:
2830 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI