################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AgeI AhdI Alw44I Aor51HI ApaLI AscI Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I CelII CfoI Cfr10I ClaI CpoI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AciI 1146 GCGG CCGC(-3/-1) AflII 481 CTTAAG C^TTAAG AflIII 1455 ACATGT A^C[GA][CT]GT AlwNI 1184 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 123 CCTCAGG CC^T[ACGT]AGG ApaI 1714 GGGCCC GGGCC^C AseI 1555 ATTAAT AT^TAAT AsnI 1555 ATTAAT AT^TAAT Asp700I 877 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 298 CCCGAG C^[CT]CG[GA]G AvaII 628 GGACC G^G[AT]CC BclI 164 TGATCA T^GATCA BcnI 1689 CCCGG CC^[GC]GG BfrI 481 CTTAAG C^TTAAG BsaWI 1140 ACCGGA [AT]^CCGG[AT] BsoBI 298 CCCGAG C^[CT]CG[GA]G BspLU11I 1455 ACATGT A^CATGT BspMI 1607 ACCTGC ACCTGC(4/8) BsrBI 1144 GAGCGG CCGCTC(-3/-3) BstEII 1603 GGTGACC G^GT[ACGT]ACC BstPI 1603 GGTGACC G^GT[ACGT]ACC BstXI 18 CCACTGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 123 CCTCAGG CC^T[ACGT]AGG Eco81I 123 CCTCAGG CC^T[ACGT]AGG EcoNI 1582 CCTGGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1603 GGTGACC G^GT[ACGT]ACC EcoRV 694 GATATC GAT^ATC FauI 1146 GCGGG CCCGC(4/6) FbaI 164 TGATCA T^GATCA HincII 1165 GTTGAC GT[CT]^[GA]AC HindII 1165 GTTGAC GT[CT]^[GA]AC MslI 458 CACAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 1689 CCCGG CC^[GC]GG NdeI 1478 CATATG CA^TATG PflMI 606 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 774 GAGTC GAGTC(4/5) PshBI 1555 ATTAAT AT^TAAT SbfI 1608 CCTGCAGG CCTGCA^GG SexAI 1254 ACCTGGT A^CC[AT]GGT Sse8387I 1608 CCTGCAGG CCTGCA^GG Tsp45I 1604 GTGAC ^GT[GC]AC Van91I 606 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1417 TCTAGA T^CTAGA XmnI 877 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AlwI 1129 GGATC GGATC(4/5) 1500 GGATC BalI 466 TGGCCA TGG^CCA 1523 TGGCCA BanII 1288 GGGCTC G[GA]GC[CT]^C 1714 GGGCCC BglII 1414 AGATCT A^GATCT 1761 AGATCT BmyI 1288 GGGCTC G[AGT]GC[ACT]^C 1714 GGGCCC BsgI 380 CTGCAC GTGCAG(16/14) 1331 GTGCAG BsmFI 1267 GGGAC GGGAC(10/14) 256 GTCCC Bsp1286I 1288 GGGCTC G[AGT]GC[ACT]^C 1714 GGGCCC BsrDI 714 CATTGC GCAATG(2/0) 1837 GCAATG BstYI 1414 AGATCT [GA]^GATC[CT] 1761 AGATCT Cac8I 1632 GCAGGC GC[ACGT]^[ACGT]GC 1683 GCTGGC DraI 535 TTTAAA TTT^AAA 1308 TTTAAA DraIII 311 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG 1253 CACCTGGTG EaeI 466 TGGCCA [CT]^GGCC[GA] 1523 TGGCCA EcoRI 411 GAATTC G^AATTC 516 GAATTC EcoT14I 243 CCAAGG C^C[AT][AT]GG 1390 CCAAGG HapII 1141 CCGG C^CGG 1690 CCGG HindIII 347 AAGCTT A^AGCTT 478 AAGCTT HpaII 1141 CCGG C^CGG 1690 CCGG MaeII 645 ACGT A^CGT 1348 ACGT MaeIII 975 GTTAC ^GT[ACGT]AC 1604 GTGAC MflI 1414 AGATCT [GA]^GATC[CT] 1761 AGATCT MluNI 466 TGGCCA TGG^CCA 1523 TGGCCA MscI 466 TGGCCA TGG^CCA 1523 TGGCCA MspA1I 100 CAGCTG C[AC]G^C[GT]G 1048 CAGCTG MspI 1141 CCGG C^CGG 1690 CCGG NspI 488 ACATGC [GA]CATG^[CT] 1455 ACATGT PstI 959 CTGCAG CTGCA^G 1609 CTGCAG PvuII 100 CAGCTG CAG^CTG 1048 CAGCTG ScaI 1517 AGTACT AGT^ACT 1748 AGTACT SfcI 959 CTGCAG C^T[GA][CT]AG 1609 CTGCAG SmlI 481 CTTAAG C^T[CT][GA]AG 1062 CTCAAG StyI 243 CCAAGG C^C[AT][AT]GG 1390 CCAAGG TaqI 344 TCGA T^CGA 1423 TCGA TthHB8I 344 TCGA T^CGA 1423 TCGA XhoII 1414 AGATCT [GA]^GATC[CT] 1761 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AcsI AfaI AluI ApoI BbsI BbvI BfaI BpuAI BsaBI BsaJI BseRI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MamI MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp509I TspRI