NME8 ORF clone pFN21AE3252

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE3252

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Clone info.

Species

human

Clone Name

pFN21AE3252 Sequence (with vector) Restriction Map

Product ID

FHC12715

ORF Sequence

DNA sequence (1764 bp)
Protein sequence (588 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3252

Symbol

NME8

Alias : CILD6, DNAI8, HEL-S-99, NM23-H8, SPTRX2, TXNDC3, sptrx-2

Description

NME/NM23 family member 8

Original Clone ID

cp93252

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1764 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 588 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5452.1 2.8e-211 99.7 NME8 CCDS4197.1 1.2e-11 38.0 NME5 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013766 14 102 PF00085 Thioredoxin domain IPR001564 156 229 PF00334 Nucleoside diphosphate kinase IPR001564 316 449 PF00334 Nucleoside diphosphate kinase IPR001564 451 584 PF00334 Nucleoside diphosphate kinase HMMSmart IPR001564 315 455 SM00562 Nucleoside diphosphate kinase IPR001564 456 588 SM00562 Nucleoside diphosphate kinase ProfileScan IPR017936 1 119 PS51352 Thioredoxin-like
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_057700 588 607421,610852 99.7 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3252 Download

>KIEE3252  1764 bp
ATGGCAAGCAAAAAACGAGAAGTCCAGTTACAGACAGTCATCAATAATCAAAGCCTGTGG
GATGAGATGTTGCAGAACAAAGGCTTAACAGTGATTGATGTTTACCAAGCCTGGTGTGGA
CCTTGCAGAGCAATGCAACCTTTATTCAGAAAATTGAAAAATGAACTGAACGAAGACGAA
ATTCTGCATTTTGCTGTCGCAGAAGCTGACAACATTGTGACTTTGCAGCCATTTAGAGAT
AAATGTGAACCTGTTTTTCTCTTTAGTGTTAATGGCAAAATTATCGAAAAGATTCAGGGT
GCAAATGCACCGCTTGTTAATAAAAAAGTTATTAATTTGATCGATGAGGAGAGAAAAATT
GCAGCAGGTGAAATGGCTCGACCTCAGTATCCTGAAATTCCATTAGTAGACTCAGATTCA
GAAGTTAGTGAAGAATCACCATGTGAAAGTGTTCAGGAATTATACAGTATTGCTATTATC
AAACCGGATGCTGTGATTAGTAAAAAAGTTCTAGAAATTAAAAGAAAAATTACCAAAGCT
GGATTTATTATAGAAGCAGAGCATAAGACAGTGCTCACTGAAGAACAAGTTGTCAACTTC
TATAGTCGAATAGCAGACCAGCGTGACTTCGAAGAGTTTGTCTCTTTTATGACAAGTGGC
TTAAGCTATATTCTAGTTGTATCTCAAGGAAGTAAACACAATCCTCCCTCTGAAGAAACC
GAACCACAGACTGACACCGAACCTAACGAACGATCTGAGGATCAACCTGAGGTCGAAGCC
CAGGTTACACCTGGAATGATGAAGAACAAACAAGACAGTTTACAAGAATATCTGGAAAGA
CAACATTTAGCTCAGCTCTGTGACATTGAAGAGGATGCAGCTAATGTTGCTAAGTTCATG
GATGCTTTCTTCCCCGATTTTAAAAAAATGAAAAGCATGAAATTAGAAAAGACATTGGCA
TTACTTCGACCAAATCTCTTTCATGAAAGGAAAGATGATGTTTTGCGTATTATTAAAGAT
GAAGACTTCAAAATACTGGAGCAAAGACAAGTAGTATTATCGGAAAAAGAAGCACAAGCA
CTGTGCAAGGAATATGAAAATGAAGACTATTTTAATAAACTTATAGAAAACATGACCAGT
GGTCCATCTCTAGCCCTTGTTTTATTGAGAGACAATGGCTTGCAATACTGGAAACAATTA
CTGGGACCAAGAACTGTTGAAGAAGCCATTGAATATTTTCCAGAGAGTTTATGTGCACAG
TTTGCGATGGACAGTTTGCCGGTCAACCAGTTGTATGGCAGCGATTCATTAGAAACCGCT
GAAAGGGAAATACAGCATTTCTTTCCTCTTCAAAGCACTTTAGGCTTGATTAAACCTCAT
GCAACAAGTGAACAAAGAGAGCAGATCCTGAAGATAGTTAAGGAGGCTGGATTTGATCTG
ACACAGGTGAAGAAAATGTTCCTAACTCCTGAGCAAACTGAGAAAATTTATCCAAAAGTA
ACAGGAAAAGACTTTTATAAAGATTTATTGGAAATGTTATCTGTGGGTCCATCTATGGTC
ATGATTCTGACCAAGTGGAATGCTGTTGCAGAATGGAGACGATTGATGGGCCCAACAGAC
CCAGAAGAAGCAAAATTACTTTCCCCTGACTCCATCCGAGCCCAGTTTGGAATAAGTAAA
TTGAAAAACATTGTCCATGGAGCATCTAACGCCTATGAAGCAAAAGAGGTTGTTAATAGA
CTCTTTGAGGATCCTGAGGAAAAC

ORF Protein

Cloned ORF protein sequence for pFN21AE3252 Download

>KIEE3252  588 aa
MASKKREVQLQTVINNQSLWDEMLQNKGLTVIDVYQAWCGPCRAMQPLFRKLKNELNEDE
ILHFAVAEADNIVTLQPFRDKCEPVFLFSVNGKIIEKIQGANAPLVNKKVINLIDEERKI
AAGEMARPQYPEIPLVDSDSEVSEESPCESVQELYSIAIIKPDAVISKKVLEIKRKITKA
GFIIEAEHKTVLTEEQVVNFYSRIADQRDFEEFVSFMTSGLSYILVVSQGSKHNPPSEET
EPQTDTEPNERSEDQPEVEAQVTPGMMKNKQDSLQEYLERQHLAQLCDIEEDAANVAKFM
DAFFPDFKKMKSMKLEKTLALLRPNLFHERKDDVLRIIKDEDFKILEQRQVVLSEKEAQA
LCKEYENEDYFNKLIENMTSGPSLALVLLRDNGLQYWKQLLGPRTVEEAIEYFPESLCAQ
FAMDSLPVNQLYGSDSLETAEREIQHFFPLQSTLGLIKPHATSEQREQILKIVKEAGFDL
TQVKKMFLTPEQTEKIYPKVTGKDFYKDLLEMLSVGPSMVMILTKWNAVAEWRRLMGPTD
PEEAKLLSPDSIRAQFGISKLKNIVHGASNAYEAKEVVNRLFEDPEEN

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3252 Download

>pFN21AE3252  6469 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCAAGCAAAAAACGAGAAGTCCAGTTACAGAC
AGTCATCAATAATCAAAGCCTGTGGGATGAGATGTTGCAGAACAAAGGCTTAACAGTGAT
TGATGTTTACCAAGCCTGGTGTGGACCTTGCAGAGCAATGCAACCTTTATTCAGAAAATT
GAAAAATGAACTGAACGAAGACGAAATTCTGCATTTTGCTGTCGCAGAAGCTGACAACAT
TGTGACTTTGCAGCCATTTAGAGATAAATGTGAACCTGTTTTTCTCTTTAGTGTTAATGG
CAAAATTATCGAAAAGATTCAGGGTGCAAATGCACCGCTTGTTAATAAAAAAGTTATTAA
TTTGATCGATGAGGAGAGAAAAATTGCAGCAGGTGAAATGGCTCGACCTCAGTATCCTGA
AATTCCATTAGTAGACTCAGATTCAGAAGTTAGTGAAGAATCACCATGTGAAAGTGTTCA
GGAATTATACAGTATTGCTATTATCAAACCGGATGCTGTGATTAGTAAAAAAGTTCTAGA
AATTAAAAGAAAAATTACCAAAGCTGGATTTATTATAGAAGCAGAGCATAAGACAGTGCT
CACTGAAGAACAAGTTGTCAACTTCTATAGTCGAATAGCAGACCAGCGTGACTTCGAAGA
GTTTGTCTCTTTTATGACAAGTGGCTTAAGCTATATTCTAGTTGTATCTCAAGGAAGTAA
ACACAATCCTCCCTCTGAAGAAACCGAACCACAGACTGACACCGAACCTAACGAACGATC
TGAGGATCAACCTGAGGTCGAAGCCCAGGTTACACCTGGAATGATGAAGAACAAACAAGA
CAGTTTACAAGAATATCTGGAAAGACAACATTTAGCTCAGCTCTGTGACATTGAAGAGGA
TGCAGCTAATGTTGCTAAGTTCATGGATGCTTTCTTCCCCGATTTTAAAAAAATGAAAAG
CATGAAATTAGAAAAGACATTGGCATTACTTCGACCAAATCTCTTTCATGAAAGGAAAGA
TGATGTTTTGCGTATTATTAAAGATGAAGACTTCAAAATACTGGAGCAAAGACAAGTAGT
ATTATCGGAAAAAGAAGCACAAGCACTGTGCAAGGAATATGAAAATGAAGACTATTTTAA
TAAACTTATAGAAAACATGACCAGTGGTCCATCTCTAGCCCTTGTTTTATTGAGAGACAA
TGGCTTGCAATACTGGAAACAATTACTGGGACCAAGAACTGTTGAAGAAGCCATTGAATA
TTTTCCAGAGAGTTTATGTGCACAGTTTGCGATGGACAGTTTGCCGGTCAACCAGTTGTA
TGGCAGCGATTCATTAGAAACCGCTGAAAGGGAAATACAGCATTTCTTTCCTCTTCAAAG
CACTTTAGGCTTGATTAAACCTCATGCAACAAGTGAACAAAGAGAGCAGATCCTGAAGAT
AGTTAAGGAGGCTGGATTTGATCTGACACAGGTGAAGAAAATGTTCCTAACTCCTGAGCA
AACTGAGAAAATTTATCCAAAAGTAACAGGAAAAGACTTTTATAAAGATTTATTGGAAAT
GTTATCTGTGGGTCCATCTATGGTCATGATTCTGACCAAGTGGAATGCTGTTGCAGAATG
GAGACGATTGATGGGCCCAACAGACCCAGAAGAAGCAAAATTACTTTCCCCTGACTCCAT
CCGAGCCCAGTTTGGAATAAGTAAATTGAAAAACATTGTCCATGGAGCATCTAACGCCTA
TGAAGCAAAAGAGGTTGTTAATAGACTCTTTGAGGATCCTGAGGAAAACGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT