(Circular) (Six-base) MAPSORT of: pFN21AE3252.seq Check: 2641 from: 1 to: 6469 pFN21AE3252 6469 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2360 2360 Size: 6469 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4279 278 Size: 53 83 186 936 2743 2468 Fragments arranged by size: 2743 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2412 3812 1937 Size: 475 1400 4594 Fragments arranged by size: 4594 1400 475 Acc65I G'GTAC_C Cuts at: 3790 3790 Size: 6469 AclI AA'CG_TT Cuts at: 1563 3768 4596 4969 1563 Size: 2205 828 373 3063 Fragments arranged by size: 3063 2205 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2604 2736 3434 4532 5916 6184 1632 Size: 972 132 698 1098 1384 268 1917 Fragments arranged by size: 1917 1384 1098 972 698 268 132 AflII C'TTAA_G Cuts at: 820 1017 2665 820 Size: 197 1648 4624 Fragments arranged by size: 4624 1648 197 AflIII A'CryG_T Cuts at: 1203 5368 6345 1203 Size: 4165 977 1327 Fragments arranged by size: 4165 1327 977 AgeI A'CCGG_T Cuts at: 5300 5300 Size: 6469 AhdI GACnn_n'nnGTC Cuts at: 3144 5198 3144 Size: 2054 4415 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6469 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2153 1184 Size: 969 5500 AlwNI CAG_nnn'CTG Cuts at: 2210 3412 4212 5784 2210 Size: 1202 800 1572 2895 Fragments arranged by size: 2895 1572 1202 800 ApaI G_GGCC'C Cuts at: 3617 3617 Size: 6469 ApaLI G'TGCA_C Cuts at: 3258 4526 5682 3258 Size: 1268 1156 4045 Fragments arranged by size: 4045 1268 1156 ApoI r'AATT_y Cuts at: 1493 2184 2400 3489 3778 4004 4156 6265 1493 Size: 691 216 1089 289 226 152 2109 1697 Fragments arranged by size: 2109 1697 1089 691 289 226 216 152 AseI AT'TA_AT Cuts at: 160 1032 2337 5023 160 Size: 872 1305 2686 1606 Fragments arranged by size: 2686 1606 1305 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 AvaI C'yCGr_G Cuts at: 1870 1943 3794 1870 Size: 73 1851 4545 Fragments arranged by size: 4545 1851 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2375 2408 2375 Size: 33 6436 BamHI G'GATC_C Cuts at: 3754 3799 4166 3754 Size: 45 367 6057 Fragments arranged by size: 6057 367 45 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3790 5245 618 Size: 325 217 627 2003 1455 1842 Fragments arranged by size: 2003 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3617 3667 3788 1391 Size: 2226 50 121 4072 Fragments arranged by size: 4072 2226 121 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2184 3033 3114 928 Size: 971 285 849 81 4283 Fragments arranged by size: 4283 971 849 285 81 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2269 2303 4660 4694 1903 Size: 34 332 34 2357 34 3678 Fragments arranged by size: 3678 2357 332 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2403 4361 5571 2403 Size: 1958 1210 3301 Fragments arranged by size: 3301 1958 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6469 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5080 136 Size: 107 122 71 631 605 3408 1525 Fragments arranged by size: 3408 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6464 6464 Size: 6469 BlpI GC'TnA_GC Cuts at: 2856 3886 2856 Size: 1030 5439 Bme1580I G_kGCm'C Cuts at: 1790 3262 3617 4530 5686 1790 Size: 1472 355 913 1156 2573 Fragments arranged by size: 2573 1472 1156 913 355 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6469 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3215 3661 4200 Size: 416 53 83 399 24 1798 446 539 Cuts at: 4200 5158 442 Size: 958 1753 Fragments arranged by size: 1798 1753 958 539 446 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6469 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2662 2662 Size: 6469 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3062 5129 1239 Size: 1823 2067 2579 Fragments arranged by size: 2579 2067 1823 Bpu10I CC'TnA_GC Cuts at: 3474 3474 Size: 6469 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2673 4594 5459 5757 2673 Size: 1921 865 298 3385 Fragments arranged by size: 3385 1921 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5132 882 Size: 617 3633 2219 Fragments arranged by size: 3633 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4260 493 Size: 711 3056 2702 Fragments arranged by size: 3056 2702 711 BsaBI GATnn'nnATC Cuts at: 6463 6463 Size: 6469 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4276 4658 5293 275 Size: 53 83 186 936 2743 382 635 1451 Fragments arranged by size: 2743 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2488 4902 5300 5574 5721 6132 2488 Size: 2414 398 274 147 411 2825 Fragments arranged by size: 2825 2414 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6244 Fragments arranged by size: 6244 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2366 1186 Size: 1180 5289 BseYI C'CCAG_C Cuts at: 5672 6168 5672 Size: 496 5973 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5896 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3919 4680 5708 1540 Size: 275 186 1918 761 1028 2301 Fragments arranged by size: 2301 1918 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2580 3262 4530 4615 5686 2580 Size: 682 1268 85 1071 3363 Fragments arranged by size: 3363 1268 1071 682 85 BsmI GAATG_Cn' Cuts at: 3589 3980 4073 3589 Size: 391 93 5985 Fragments arranged by size: 5985 391 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3595 3595 Size: 6469 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2580 3262 3617 3667 3788 4530 4615 Size: 399 790 682 355 50 121 742 85 Cuts at: 4615 5686 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 790 742 682 399 355 121 85 50 BspEI T'CCGG_A Cuts at: 6132 6132 Size: 6469 BspHI T'CATG_A Cuts at: 2986 3564 4358 6088 2986 Size: 578 794 1730 3367 Fragments arranged by size: 3367 1730 794 578 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2360 3824 844 Size: 1516 1464 3489 Fragments arranged by size: 3489 1516 1464 BsrBI CCG'CTC Cuts at: 1394 1823 4356 1394 Size: 429 2533 3507 Fragments arranged by size: 3507 2533 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2142 4964 5138 65 Size: 1183 894 2822 174 1396 Fragments arranged by size: 2822 1396 1183 894 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3283 5113 5300 1700 Size: 110 1473 1830 187 2869 Fragments arranged by size: 2869 1830 1473 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6469 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6469 BssSI C'ACGA_G Cuts at: 4529 5541 4529 Size: 1012 5457 BstAPI GCAn_nnn'nTGC Cuts at: 794 3266 794 Size: 2472 3997 BstBI TT'CG_AA Cuts at: 2634 5273 2634 Size: 2639 3830 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6469 BstXI CCAn_nnnn'nTGG Cuts at: 3299 3299 Size: 6469 BstYI r'GATC_y Cuts at: 1972 3408 3754 3799 4166 4551 4568 6020 6106 Size: 1436 346 45 367 385 17 1452 86 Cuts at: 6106 6118 6464 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1436 385 367 346 346 86 45 17 12 Bsu36I CC'TnA_GG Cuts at: 2772 3759 2772 Size: 987 5482 BtgI C'CryG_G Cuts at: 513 1065 2004 3700 513 Size: 552 939 1696 3282 Fragments arranged by size: 3282 1696 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3284 487 Size: 683 56 2058 3672 Fragments arranged by size: 3672 2058 683 56 BtsI GCAGTG_nn' Cuts at: 3985 4779 4799 3985 Size: 794 20 5655 Fragments arranged by size: 5655 794 20 ClaI AT'CG_AT Cuts at: 1554 2346 1554 Size: 792 5677 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2042 2077 529 Size: 35 1478 35 4921 Fragments arranged by size: 4921 1478 35 35 DraI TTT'AAA Cuts at: 2926 3773 2926 Size: 847 5622 DraIII CAC_nnn'GTG Cuts at: 2447 2447 Size: 6469 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5476 809 Size: 507 4160 1802 Fragments arranged by size: 4160 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3916 4805 6434 Size: 54 1425 50 275 18 2086 889 1629 Cuts at: 6434 8 Size: 43 Fragments arranged by size: 2086 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3916 1537 Size: 275 2104 4090 Fragments arranged by size: 4090 2104 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2631 2868 3357 4399 1353 Size: 1278 237 489 1042 3423 Fragments arranged by size: 3423 1278 1042 489 237 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5056 5430 5576 387 Size: 1064 138 3467 374 146 1280 Fragments arranged by size: 3467 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2604 2736 3062 3434 4532 5129 5916 Size: 393 972 132 326 372 1098 597 787 Cuts at: 5916 6184 1239 Size: 268 1524 Fragments arranged by size: 1524 1098 972 787 597 393 372 326 268 132 EcoRI G'AATT_C Cuts at: 3778 4156 3778 Size: 378 6091 FspI TGC'GCA Cuts at: 4975 4975 Size: 6469 HaeII r_GCGC'y Cuts at: 1678 5616 1678 Size: 3938 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2964 2996 3130 3162 3364 Size: 32 135 32 1392 32 134 32 202 Cuts at: 3364 3393 3396 3425 5110 5142 5184 5216 1373 Size: 29 3 29 1685 32 42 32 2626 Fragments arranged by size: 2626 1685 1392 202 135 134 42 32 32 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 2599 3289 3813 4059 669 Size: 988 281 661 690 524 246 3079 Fragments arranged by size: 3079 988 690 661 524 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6469 HpaI GTT'AAC Cuts at: 4059 4059 Size: 6469 KpnI G_GTAC'C Cuts at: 3794 3794 Size: 6469 MfeI C'AATT_G Cuts at: 4068 4068 Size: 6469 MluI A'CGCG_T Cuts at: 6345 6345 Size: 6469 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5583 5767 1305 Size: 4278 184 2007 Fragments arranged by size: 4278 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4647 Fragments arranged by size: 4647 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4427 4786 4945 518 Size: 648 3261 359 159 2042 Fragments arranged by size: 3261 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3323 3885 4562 5710 5955 1606 Size: 1717 562 677 1148 245 2120 Fragments arranged by size: 2120 1717 1148 677 562 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3700 513 Size: 552 939 1696 3282 Fragments arranged by size: 3282 1696 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6469 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6469 NotI GC'GGCC_GC Cuts at: 3916 3916 Size: 6469 NspI r_CATG'y Cuts at: 1132 1229 3827 5372 1132 Size: 97 2598 1545 2229 Fragments arranged by size: 2598 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6469 PciI A'CATG_T Cuts at: 5368 5368 Size: 6469 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6469 PmeI GTTT'AAAC Cuts at: 3773 3773 Size: 6469 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4538 4570 6051 6083 4538 Size: 32 1481 32 4924 Fragments arranged by size: 4924 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6469 PsiI TTA'TAA Cuts at: 3522 4039 3522 Size: 517 5952 PspOMI G'GGCC_C Cuts at: 3613 3613 Size: 6469 PstI C_TGCA'G Cuts at: 830 3821 830 Size: 2991 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6469 SalI G'TCGA_C Cuts at: 1936 3811 1936 Size: 1875 4594 SbfI CC_TGCA'GG Cuts at: 3821 3821 Size: 6469 ScaI AGT'ACT Cuts at: 4717 6429 4717 Size: 1712 4757 SfcI C'TryA_G Cuts at: 826 1045 2605 3817 4952 5633 5824 826 Size: 219 1560 1212 1135 681 191 1471 Fragments arranged by size: 1560 1471 1212 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6469 SmaI CCC'GGG Cuts at: 1872 3796 1872 Size: 1924 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2665 2688 4573 5474 5736 820 Size: 197 926 722 23 1885 901 262 1553 Fragments arranged by size: 1885 1553 926 901 722 262 197 23 SnaBI TAC'GTA Cuts at: 493 4260 493 Size: 3767 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6469 SphI G_CATG'C Cuts at: 1132 3827 1132 Size: 2695 3774 SspI AAT'ATT Cuts at: 5 52 3239 4393 5 Size: 47 3187 1154 2081 Fragments arranged by size: 3187 2081 1154 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6469 StyI C'CwwG_G Cuts at: 513 1065 2004 3700 3908 513 Size: 552 939 1696 208 3074 Fragments arranged by size: 3074 1696 939 552 208 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4496 4513 4666 4851 5296 325 Size: 1255 2916 17 153 185 445 1498 Fragments arranged by size: 2916 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4715 6427 96 Size: 274 80 33 51 1443 2738 1712 138 Fragments arranged by size: 2738 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6429 6461 6429 Size: 32 6437 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6469 XbaI T'CTAG_A Cuts at: 2515 3805 2515 Size: 1290 5179 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2117 2792 2117 Size: 675 5794 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6469 XmaI C'CCGG_G Cuts at: 1870 3794 1870 Size: 1924 4545 XmnI GAAnn'nnTTC Cuts at: 2295 2454 3240 3461 4598 2295 Size: 159 786 221 1137 4166 Fragments arranged by size: 4166 1137 786 221 159 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4277 276 Size: 53 83 186 936 2743 2468 Fragments arranged by size: 2743 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BfrBI BsiWI BstZ17I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI