################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AfaI AflIII AgeI Aor51HI AscI Asp718I AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbvCI BclI BcnI BglI BglII BlnI BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsoBI Bsp1407I BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstUI BstZ17I CfoI CpoI DraII DrdI EaeI EagI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 406 GTAGAC GT^[AC][GT]AC AflII 660 CTTAAG C^TTAAG AhdI 1134 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1253 GTGCAC G^TGCAC ApaI 1608 GGGCCC GGGCC^C ApaLI 1253 GTGCAC G^TGCAC AseI 331 ATTAAT AT^TAAT AsnI 331 ATTAAT AT^TAAT AspEI 1134 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 1749 GGATCC G^GATCC BcgI 275 GCAAAATTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 387 GTATCC GTATCC(6/5) BfrI 660 CTTAAG C^TTAAG BlpI 850 GCTCAGC GC^T[ACGT]AGC BpmI 1036 CTGGAG CTGGAG(16/14) Bpu1102I 850 GCTCAGC GC^T[ACGT]AGC BsaWI 483 ACCGGA [AT]^CCGG[AT] BseRI 346 GAGGAG GAGGAG(10/8) BsiYI 1316 CCGCTGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 1316 CCGCTGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 1596 GAGACG CGTCTC(1/5) BsmFI 1203 GGGAC GGGAC(10/14) BsmI 1578 GAATGC GAATGC(1/-1) BspDI 340 ATCGAT AT^CGAT BspMI 364 GCAGGT ACCTGC(4/8) BsrDI 130 GCAATG GCAATG(2/0) BsrFI 1278 GCCGGT [GA]^CCGG[CT] BstAPI 1255 GCACAGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 628 TTCGAA TT^CGAA BstXI 1287 CCAGTTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 850 GCTCAGC GC^T[ACGT]AGC Cfr10I 1278 GCCGGT [GA]^CCGG[CT] ClaI 340 ATCGAT AT^CGAT DraI 919 TTTAAA TTT^AAA DraIII 437 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1695 CCATGG C^C[GA][CT]GG Eam1105I 1134 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT14I 1695 CCATGG C^C[AT][AT]GG HaeIII 1609 GGCC GG^CC MspA1I 1316 CCGCTG C[AC]G^C[GT]G NcoI 1695 CCATGG C^CATGG NspV 628 TTCGAA TT^CGAA PshBI 331 ATTAAT AT^TAAT SfcI 600 CTATAG C^T[GA][CT]AG SfuI 628 TTCGAA TT^CGAA SspI 1232 AATATT AAT^ATT StyI 1695 CCATGG C^C[AT][AT]GG XbaI 510 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AciI 310 CCGC CCGC(-3/-1) 1316 CCGC AlwNI 200 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1402 CAGATCCTG AocI 766 CCTGAGG CC^T[ACGT]AGG 1753 CCTGAGG AspHI 571 GTGCTC G[AT]GC[AT]^C 1253 GTGCAC BanII 1608 GGGCCC G[GA]GC[CT]^C 1658 GAGCCC BsaJI 779 CCCAGG C^C[ACGT][ACGT]GG 1695 CCATGG BsiHKAI 571 GTGCTC G[AT]GC[AT]^C 1253 GTGCAC BspHI 981 TCATGA T^CATGA 1559 TCATGA BstYI 1403 AGATCC [GA]^GATC[CT] 1749 GGATCC Bsu36I 766 CCTGAGG CC^T[ACGT]AGG 1753 CCTGAGG Cac8I 4 GCAAGC GC[ACGT]^[ACGT]GC 1178 GCTTGC Eco81I 766 CCTGAGG CC^T[ACGT]AGG 1753 CCTGAGG HapII 484 CCGG C^CGG 1279 CCGG HincII 592 GTCAAC GT[CT]^[GA]AC 1282 GTCAAC HindII 592 GTCAAC GT[CT]^[GA]AC 1282 GTCAAC HpaII 484 CCGG C^CGG 1279 CCGG MflI 1403 AGATCC [GA]^GATC[CT] 1749 GGATCC MspI 484 CCGG C^CGG 1279 CCGG RcaI 981 TCATGA T^CATGA 1559 TCATGA SmlI 660 CTTAAG C^T[CT][GA]AG 683 CTCAAG XcmI 105 CCAAGCCTGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 780 CCAGGTTACACCTGG XhoII 1403 AGATCC [GA]^GATC[CT] 1749 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AcsI AluI AlwI ApoI Asp700I AvaII BbsI BbvI BfaI BmyI BpuAI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI