TRMT2B ORF clone pFN21AE3222

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE3222

How to Order Order Information

Clone info.

Species

human

Clone Name

pFN21AE3222 Sequence (with vector) Restriction Map

Product ID

FHC11686

ORF Sequence

DNA sequence (1512 bp)
Protein sequence (504 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3222

Symbol

TRMT2B

Alias : CXorf34, dJ341D10.3

Description

tRNA methyltransferase 2 homolog B (S. cerevisiae), transcript variant 1

Original Clone ID

cp93222

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1512 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 504 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS14477.1 0 100.0 TRMT2B CCDS55464.1 3.5e-155 91.1 TRMT2B CCDS13774.1 1.3e-64 37.3 TRMT2A CCDS58793.1 2.7e-56 36.2 TRMT2A CCDS82693.1 3e-38 36.2 TRMT2A The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR010280 314 454 PF05958 (Uracil-5)-methyltransferase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_073564 625 611151 37.3 100.0 NP_892029 625 611151 37.3 100.0 NP_001244923 562 611151 36.2 93.3 NP_001317968 643 611151 36.2 100.0 XP_011528441 643 611151 36.2 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3222 Download

>KIEE3222  1512 bp
ATGGCAGGCCTTAAGAGAAGAGTCCCACTGCACAGCCTCAGATACTTCATCTCCATGGTG
GGTCTCTTCTCCAAACCAGGACTGCTTCCCTGGTATGCCAGAAATCCACCAGGATGGTCA
CAGCTCTTTCTGGGCACAGTATGTAAGGGAGATTTCACCCGTGTGATAGCCACGAAATGT
CAGAAAGGACAAAAAAGTCAGAAGAAACCAAGCCATCTTGGACCACTAGATGGTTCCTGG
CAGGAAAGGCTGGCTGATGTTGTGACACCACTCTGGAGGTTGAGCTATGAAGAACAGCTC
AAGGTGAAATTTGCAGCTCAGAAGAAAATTTTACAAAGACTAGAGTCTTACATCCAAATG
CTCAATGGAGTCAGTGTGACAACGGCTGTACCCAAATCTGAGAGGCTCTCTTGTCTTCTC
CATCCTATTATACCCTCTCCTGTCATCAATGGTTACCGAAATAAGTCCACCTTCTCTGTG
AACCGAGGTCCAGATGGCAATCCAAAGACTGTGGGGTTCTACCTGGGAACTTGGAGAGAT
GGGAACGTTGTCTGTGTGCAGTCTAATCATCTGAAAAACATCCCTGAGAAACACAGTCAA
GTGGCGCAGTACTATGAAGTATTCCTTCGACAGTCTCCATTGGAGCCCTGCCTTGTATTT
CATGAAGGTGGATACTGGCGTGAGCTCACAGTCCGCACCAATAGCCAAGGGCACACAATG
GCTATCATCACTTTCCATCCCCAGAAATTAAGTCAGGAGGAGCTCCATGTTCAGAAGGAG
ATTGTAAAGGAATTTTTCATCAGAGGTCCTGGAGCAGCCTGTGGCTTGACCTCACTTTAC
TTCCAGGAAAGTACCATGACCCGTTGCAGCCATCAGCAGTCTCCCTATCAGCTTCTGTTT
GGGGAACCCTACATCTTTGAAGAACTTCTGAGCTTGAAGATCCGCATCTCTCCAGATGCC
TTTTTCCAGATTAACACAGCTGGTGCAGAGATGCTGTATCGGACTGTGGGGGAGCTGACT
GGAGTGAACTCTGACACCATCCTTCTTGACATCTGCTGTGGAACTGGTGTGATTGGCCTC
TCTCTGGCTCAGCATACATCTCGGGTCCTTGGGATTGAATTGTTGGAGCAGGCAGTGGAG
GATGCAAGATGGACTGCAGCCTTCAATGGCATCACCAACTCTGAATTTCATACTGGTCAA
GCAGAGAAGATTTTGCCAGGGCTGCTAAAGTCAAAGGAAGATGGACAGTCAATTGTTGCT
GTGGTGAACCCAGCCCGTGCCGGACTGCATTACAAGGTGATTCAAGCCATTCGAAACTTC
AGGGCCATCCACACGCTAGTTTTTGTTTCCTGCAAGCTCCATGGTGAATCCACTAGGAAT
GTCATTGAGCTGTGCTGTCCTCCAGACCCTGCTAAGAAGCTCTTAGGCGAGCCCTTTGTC
CTGCAGCAAGCTGTACCTGTGGATTTGTTCCCTCACACCCCACATTGTGAGCTGGTGCTC
CTCTTTACTCGA

ORF Protein

Cloned ORF protein sequence for pFN21AE3222 Download

>KIEE3222  504 aa
MAGLKRRVPLHSLRYFISMVGLFSKPGLLPWYARNPPGWSQLFLGTVCKGDFTRVIATKC
QKGQKSQKKPSHLGPLDGSWQERLADVVTPLWRLSYEEQLKVKFAAQKKILQRLESYIQM
LNGVSVTTAVPKSERLSCLLHPIIPSPVINGYRNKSTFSVNRGPDGNPKTVGFYLGTWRD
GNVVCVQSNHLKNIPEKHSQVAQYYEVFLRQSPLEPCLVFHEGGYWRELTVRTNSQGHTM
AIITFHPQKLSQEELHVQKEIVKEFFIRGPGAACGLTSLYFQESTMTRCSHQQSPYQLLF
GEPYIFEELLSLKIRISPDAFFQINTAGAEMLYRTVGELTGVNSDTILLDICCGTGVIGL
SLAQHTSRVLGIELLEQAVEDARWTAAFNGITNSEFHTGQAEKILPGLLKSKEDGQSIVA
VVNPARAGLHYKVIQAIRNFRAIHTLVFVSCKLHGESTRNVIELCCPPDPAKKLLGEPFV
LQQAVPVDLFPHTPHCELVLLFTR

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3222 Download

>pFN21AE3222  6217 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCAGGCCTTAAGAGAAGAGTCCCACTGCACAG
CCTCAGATACTTCATCTCCATGGTGGGTCTCTTCTCCAAACCAGGACTGCTTCCCTGGTA
TGCCAGAAATCCACCAGGATGGTCACAGCTCTTTCTGGGCACAGTATGTAAGGGAGATTT
CACCCGTGTGATAGCCACGAAATGTCAGAAAGGACAAAAAAGTCAGAAGAAACCAAGCCA
TCTTGGACCACTAGATGGTTCCTGGCAGGAAAGGCTGGCTGATGTTGTGACACCACTCTG
GAGGTTGAGCTATGAAGAACAGCTCAAGGTGAAATTTGCAGCTCAGAAGAAAATTTTACA
AAGACTAGAGTCTTACATCCAAATGCTCAATGGAGTCAGTGTGACAACGGCTGTACCCAA
ATCTGAGAGGCTCTCTTGTCTTCTCCATCCTATTATACCCTCTCCTGTCATCAATGGTTA
CCGAAATAAGTCCACCTTCTCTGTGAACCGAGGTCCAGATGGCAATCCAAAGACTGTGGG
GTTCTACCTGGGAACTTGGAGAGATGGGAACGTTGTCTGTGTGCAGTCTAATCATCTGAA
AAACATCCCTGAGAAACACAGTCAAGTGGCGCAGTACTATGAAGTATTCCTTCGACAGTC
TCCATTGGAGCCCTGCCTTGTATTTCATGAAGGTGGATACTGGCGTGAGCTCACAGTCCG
CACCAATAGCCAAGGGCACACAATGGCTATCATCACTTTCCATCCCCAGAAATTAAGTCA
GGAGGAGCTCCATGTTCAGAAGGAGATTGTAAAGGAATTTTTCATCAGAGGTCCTGGAGC
AGCCTGTGGCTTGACCTCACTTTACTTCCAGGAAAGTACCATGACCCGTTGCAGCCATCA
GCAGTCTCCCTATCAGCTTCTGTTTGGGGAACCCTACATCTTTGAAGAACTTCTGAGCTT
GAAGATCCGCATCTCTCCAGATGCCTTTTTCCAGATTAACACAGCTGGTGCAGAGATGCT
GTATCGGACTGTGGGGGAGCTGACTGGAGTGAACTCTGACACCATCCTTCTTGACATCTG
CTGTGGAACTGGTGTGATTGGCCTCTCTCTGGCTCAGCATACATCTCGGGTCCTTGGGAT
TGAATTGTTGGAGCAGGCAGTGGAGGATGCAAGATGGACTGCAGCCTTCAATGGCATCAC
CAACTCTGAATTTCATACTGGTCAAGCAGAGAAGATTTTGCCAGGGCTGCTAAAGTCAAA
GGAAGATGGACAGTCAATTGTTGCTGTGGTGAACCCAGCCCGTGCCGGACTGCATTACAA
GGTGATTCAAGCCATTCGAAACTTCAGGGCCATCCACACGCTAGTTTTTGTTTCCTGCAA
GCTCCATGGTGAATCCACTAGGAATGTCATTGAGCTGTGCTGTCCTCCAGACCCTGCTAA
GAAGCTCTTAGGCGAGCCCTTTGTCCTGCAGCAAGCTGTACCTGTGGATTTGTTCCCTCA
CACCCCACATTGTGAGCTGGTGCTCCTCTTTACTCGAGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT