(Circular) (Six-base) MAPSORT of: pFN21AE3222.seq Check: 3112 from: 1 to: 6217 pFN21AE3222 6217 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4027 278 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3560 1937 Size: 1623 4594 Acc65I G'GTAC_C Cuts at: 3538 3538 Size: 6217 AclI AA'CG_TT Cuts at: 1563 2550 4344 4717 1563 Size: 987 1794 373 3063 Fragments arranged by size: 3063 1794 987 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3307 4280 5664 5932 1632 Size: 1675 973 1384 268 1917 Fragments arranged by size: 1917 1675 1384 973 268 AflII C'TTAA_G Cuts at: 820 1017 2015 820 Size: 197 998 5022 Fragments arranged by size: 5022 998 197 AflIII A'CryG_T Cuts at: 1203 5116 6093 1203 Size: 3913 977 1327 Fragments arranged by size: 3913 1327 977 AgeI A'CCGG_T Cuts at: 5048 5048 Size: 6217 AhdI GACnn_n'nnGTC Cuts at: 4946 4946 Size: 6217 AleI CACnn'nnGTG Cuts at: 1166 2165 1166 Size: 999 5218 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6217 AlwNI CAG_nnn'CTG Cuts at: 2899 3413 3960 5532 2899 Size: 514 547 1572 3584 Fragments arranged by size: 3584 1572 547 514 ApaLI G'TGCA_C Cuts at: 4274 5430 4274 Size: 1156 5061 ApoI r'AATT_y Cuts at: 1493 2312 2331 2795 3188 3526 3752 3904 6013 Size: 819 19 464 393 338 226 152 2109 Cuts at: 6013 1493 Size: 1697 Fragments arranged by size: 2109 1697 819 464 393 338 226 152 19 AseI AT'TA_AT Cuts at: 160 1032 4771 160 Size: 872 3739 1606 Fragments arranged by size: 3739 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 AvaI C'yCGr_G Cuts at: 1870 1943 3105 3513 3542 1870 Size: 73 1162 408 29 4545 Fragments arranged by size: 4545 1162 408 73 29 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2376 2409 2668 2701 2376 Size: 33 259 33 5892 Fragments arranged by size: 5892 259 33 33 BamHI G'GATC_C Cuts at: 3547 3914 3547 Size: 367 5850 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3538 4993 618 Size: 325 217 627 1751 1455 1842 Fragments arranged by size: 1842 1751 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2652 2691 2769 3438 3536 1391 Size: 1261 39 78 669 98 4072 Fragments arranged by size: 4072 1261 669 98 78 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2411 928 Size: 971 512 4734 Fragments arranged by size: 4734 971 512 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3086 3120 4408 4442 1903 Size: 34 1149 34 1288 34 3678 Fragments arranged by size: 3678 1288 1149 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2669 4109 5319 2669 Size: 1440 1210 3567 Fragments arranged by size: 3567 1440 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6217 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4828 136 Size: 107 122 71 631 605 3156 1525 Fragments arranged by size: 3156 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6212 6212 Size: 6217 BlpI GC'TnA_GC Cuts at: 3093 3634 3093 Size: 541 5676 Bme1580I G_kGCm'C Cuts at: 1790 2141 2718 4278 5434 1790 Size: 351 577 1560 1156 2573 Fragments arranged by size: 2573 1560 1156 577 351 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6217 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3948 4906 442 Size: 416 53 83 399 24 2531 958 1753 Fragments arranged by size: 2531 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6217 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2428 2428 Size: 6217 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2299 2835 2940 3045 3390 4877 1239 Size: 1060 536 105 105 345 1487 2579 Fragments arranged by size: 2579 1487 1060 536 345 105 105 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2288 4342 5207 5505 2288 Size: 2054 865 298 3000 Fragments arranged by size: 3000 2054 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2072 4880 882 Size: 617 573 2808 2219 Fragments arranged by size: 2808 2219 617 573 BsaAI yAC'GTr Cuts at: 493 1204 4008 493 Size: 711 2804 2702 Fragments arranged by size: 2804 2702 711 BsaBI GATnn'nnATC Cuts at: 2502 2948 6211 2502 Size: 446 3263 2508 Fragments arranged by size: 3263 2508 446 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4024 4406 5041 275 Size: 53 83 186 936 2491 382 635 1451 Fragments arranged by size: 2491 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4650 5048 5322 5469 5880 4650 Size: 398 274 147 411 4987 Fragments arranged by size: 4987 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2039 2069 2364 2394 1178 Size: 30 165 30 636 30 295 30 5001 Fragments arranged by size: 5001 636 295 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2777 3494 1186 Size: 1591 717 3909 Fragments arranged by size: 3909 1591 717 BseYI C'CCAG_C Cuts at: 3274 5420 5916 3274 Size: 2146 496 3575 Fragments arranged by size: 3575 2146 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2018 2582 3009 1163 Size: 573 282 564 427 4371 Fragments arranged by size: 4371 573 564 427 282 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3667 4428 5456 1540 Size: 275 186 1666 761 1028 2301 Fragments arranged by size: 2301 1666 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2691 2769 3504 4278 4363 5434 2691 Size: 78 735 774 85 1071 3474 Fragments arranged by size: 3474 1071 774 735 85 78 BsmI GAATG_Cn' Cuts at: 3728 3821 3728 Size: 93 6124 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2141 2652 2691 2718 2769 3438 3504 Size: 399 351 511 39 27 51 669 66 Cuts at: 3504 3536 4278 4363 5434 1391 Size: 32 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 669 511 399 351 85 66 51 39 32 27 BspEI T'CCGG_A Cuts at: 5880 5880 Size: 6217 BspHI T'CATG_A Cuts at: 2665 4106 5836 2665 Size: 1441 1730 3046 Fragments arranged by size: 3046 1730 1441 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3572 844 Size: 2728 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4104 1394 Size: 429 2281 3507 Fragments arranged by size: 3507 2281 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4712 4886 65 Size: 1183 3464 174 1396 Fragments arranged by size: 3464 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4861 5048 1700 Size: 110 3051 187 2869 Fragments arranged by size: 3051 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6217 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6217 BssSI C'ACGA_G Cuts at: 4277 5289 4277 Size: 1012 5205 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6217 BstBI TT'CG_AA Cuts at: 3316 5021 3316 Size: 1705 4512 BstEII G'GTnAC_C Cuts at: 1184 2456 1184 Size: 1272 4945 BstXI CCAn_nnnn'nTGG Cuts at: 2118 2118 Size: 6217 BstYI r'GATC_y Cuts at: 1972 2943 3547 3914 4299 4316 5768 5854 5866 Size: 971 604 367 385 17 1452 86 12 Cuts at: 5866 6212 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 971 604 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2058 3364 513 Size: 552 939 54 1306 3366 Fragments arranged by size: 3366 1306 939 552 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5478 Fragments arranged by size: 5478 683 56 BtsI GCAGTG_nn' Cuts at: 2030 3144 3733 4527 4547 2030 Size: 1114 589 794 20 3700 Fragments arranged by size: 3700 1114 794 589 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6217 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2496 2531 529 Size: 35 1932 35 4215 Fragments arranged by size: 4215 1932 35 35 DraI TTT'AAA Cuts at: 3521 3521 Size: 6217 DraIII CAC_nnn'GTG Cuts at: 3491 3491 Size: 6217 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2199 5224 809 Size: 507 883 3025 1802 Fragments arranged by size: 3025 1802 883 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3664 4553 6182 Size: 54 1425 50 275 18 1834 889 1629 Cuts at: 6182 8 Size: 43 Fragments arranged by size: 1834 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3664 1537 Size: 275 1852 4090 Fragments arranged by size: 4090 1852 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2017 2075 4147 1353 Size: 664 58 2072 3423 Fragments arranged by size: 3423 2072 664 58 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4804 5178 5324 387 Size: 1064 138 3215 374 146 1280 Fragments arranged by size: 3215 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2689 2767 2689 Size: 78 6139 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2299 2835 2940 3045 3307 3390 4280 Size: 393 667 536 105 105 262 83 890 Cuts at: 4280 4877 5664 5932 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 890 787 667 597 536 393 268 262 105 105 83 EcoO109I rG'GnC_Cy Cuts at: 2810 3109 2810 Size: 299 5918 EcoRI G'AATT_C Cuts at: 3526 3904 3526 Size: 378 5839 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2491 2645 3442 2491 Size: 154 797 5266 Fragments arranged by size: 5266 797 154 FspI TGC'GCA Cuts at: 4723 4723 Size: 6217 HaeII r_GCGC'y Cuts at: 1678 5364 1678 Size: 3686 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2037 2069 2986 3018 3236 Size: 32 135 32 465 32 917 32 218 Cuts at: 3236 3268 4858 4890 4932 4964 1373 Size: 32 1590 32 42 32 2626 Fragments arranged by size: 2626 1590 917 465 218 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3561 3807 669 Size: 988 281 1623 246 3079 Fragments arranged by size: 3079 1623 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6217 HpaI GTT'AAC Cuts at: 3807 3807 Size: 6217 KpnI G_GTAC'C Cuts at: 3542 3542 Size: 6217 MfeI C'AATT_G Cuts at: 3255 3816 3255 Size: 561 5656 MluI A'CGCG_T Cuts at: 6093 6093 Size: 6217 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3108 5331 5515 1305 Size: 1803 2223 184 2007 Fragments arranged by size: 2223 2007 1803 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4395 Fragments arranged by size: 4395 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2057 2116 2165 2360 2670 4175 4534 Size: 648 891 59 49 195 310 1505 359 Cuts at: 4534 4693 518 Size: 159 2042 Fragments arranged by size: 2042 1505 891 648 359 310 195 159 59 49 MspA1I CmG'CkG Cuts at: 1606 2984 3633 4310 5458 5703 1606 Size: 1378 649 677 1148 245 2120 Fragments arranged by size: 2120 1378 1148 677 649 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2058 3364 513 Size: 552 939 54 1306 3366 Fragments arranged by size: 3366 1306 939 552 54 NdeI CA'TA_TG Cuts at: 387 387 Size: 6217 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6217 NotI GC'GGCC_GC Cuts at: 3664 3664 Size: 6217 NspI r_CATG'y Cuts at: 1132 1229 3575 5120 1132 Size: 97 2346 1545 2229 Fragments arranged by size: 2346 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6217 PciI A'CATG_T Cuts at: 5116 5116 Size: 6217 PflMI CCAn_nnn'nTGG Cuts at: 1108 2234 1108 Size: 1126 5091 PfoI T'CCnGG_A Cuts at: 2812 2847 2812 Size: 35 6182 PmeI GTTT'AAAC Cuts at: 3521 3521 Size: 6217 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4286 4318 5799 5831 4286 Size: 32 1481 32 4672 Fragments arranged by size: 4672 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2810 3109 2810 Size: 299 5918 PshAI GACnn'nnGTC Cuts at: 1613 2347 1613 Size: 734 5483 PsiI TTA'TAA Cuts at: 3787 3787 Size: 6217 PspXI vC'TCGA_Gb Cuts at: 3513 3513 Size: 6217 PstI C_TGCA'G Cuts at: 830 3163 3450 3569 830 Size: 2333 287 119 3478 Fragments arranged by size: 3478 2333 287 119 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6217 PvuII CAG'CTG Cuts at: 2984 2984 Size: 6217 SacI G_AGCT'C Cuts at: 2691 2769 2691 Size: 78 6139 SalI G'TCGA_C Cuts at: 1936 3559 1936 Size: 1623 4594 SbfI CC_TGCA'GG Cuts at: 3569 3569 Size: 6217 ScaI AGT'ACT Cuts at: 2615 4465 6177 2615 Size: 1850 1712 2655 Fragments arranged by size: 2655 1850 1712 SfcI C'TryA_G Cuts at: 826 1045 3159 3446 3565 4700 5381 5572 826 Size: 219 2114 287 119 1135 681 191 1471 Fragments arranged by size: 2114 1471 1135 681 287 219 191 119 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6217 SmaI CCC'GGG Cuts at: 1872 3544 1872 Size: 1672 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2015 2303 3513 4321 5222 5484 Size: 197 926 72 288 1210 808 901 262 Cuts at: 5484 820 Size: 1553 Fragments arranged by size: 1553 1210 926 901 808 288 262 197 72 SnaBI TAC'GTA Cuts at: 493 4008 493 Size: 3515 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6217 SphI G_CATG'C Cuts at: 1132 3575 1132 Size: 2443 3774 SspI AAT'ATT Cuts at: 5 52 4141 5 Size: 47 4089 2081 Fragments arranged by size: 4089 2081 47 StuI AGG'CCT Cuts at: 1517 2013 1517 Size: 496 5721 StyI C'CwwG_G Cuts at: 513 1065 2004 2058 2710 3112 3364 3656 513 Size: 552 939 54 652 402 252 292 3074 Fragments arranged by size: 3074 939 652 552 402 292 252 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4244 4261 4414 4599 5044 325 Size: 1255 2664 17 153 185 445 1498 Fragments arranged by size: 2664 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2613 4463 6175 Size: 274 80 33 51 1443 636 1850 1712 Cuts at: 6175 96 Size: 138 Fragments arranged by size: 1850 1712 1443 636 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2364 2396 2725 2757 2829 2861 3057 3089 6177 Size: 32 329 32 72 32 196 32 3088 Cuts at: 6177 6209 2364 Size: 32 2372 Fragments arranged by size: 3088 2372 329 196 72 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6217 XbaI T'CTAG_A Cuts at: 3553 3553 Size: 6217 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2366 2366 Size: 6217 XhoI C'TCGA_G Cuts at: 1943 3513 1943 Size: 1570 4647 XmaI C'CCGG_G Cuts at: 1870 3542 1870 Size: 1672 4545 XmnI GAAnn'nnTTC Cuts at: 2928 4346 2928 Size: 1418 4799 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4025 276 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI