(Linear) (Six-base) MAPSORT of: KIEE3222.seq Check: 6452 from: 1 to: 1512 KIEE3222.orf With 182 enzymes: SgfI * July 3, 2012 17:29 .. AclI AA'CG_TT Cuts at: 0 545 1512 Size: 545 967 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1302 1512 Size: 1302 210 AflII C'TTAA_G Cuts at: 0 10 1512 Size: 10 1502 AleI CACnn'nnGTG Cuts at: 0 160 1512 Size: 160 1352 AlwNI CAG_nnn'CTG Cuts at: 0 894 1408 1512 Size: 894 514 104 Fragments arranged by size: 894 514 104 ApoI r'AATT_y Cuts at: 0 307 326 790 1183 1512 Size: 307 19 464 393 329 Fragments arranged by size: 464 393 329 307 19 AvaI C'yCGr_G Cuts at: 0 1100 1512 Size: 1100 412 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 371 404 663 696 1512 Size: 371 33 259 33 816 Fragments arranged by size: 816 371 259 33 33 BanII G_rGCy'C Cuts at: 0 647 686 764 1433 1512 Size: 647 39 78 669 79 Fragments arranged by size: 669 647 79 78 39 BbsI GAAGACnn'nnnn_ Cuts at: 0 406 1512 Size: 406 1106 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1081 1115 1512 Size: 1081 34 397 Fragments arranged by size: 1081 397 34 BciVI GTATCCnnnnn_n' Cuts at: 0 664 1512 Size: 664 848 BlpI GC'TnA_GC Cuts at: 0 1088 1512 Size: 1088 424 Bme1580I G_kGCm'C Cuts at: 0 136 713 1512 Size: 136 577 799 Fragments arranged by size: 799 577 136 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 423 1512 Size: 423 1089 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 294 830 935 1040 1385 1512 Size: 294 536 105 105 345 127 Fragments arranged by size: 536 345 294 127 105 105 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 283 1512 Size: 283 1229 BsaI GGTCTCn'nnnn_ Cuts at: 0 67 1512 Size: 67 1445 BsaBI GATnn'nnATC Cuts at: 0 497 943 1512 Size: 497 446 569 Fragments arranged by size: 569 497 446 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 34 64 359 389 1512 Size: 34 30 295 30 1123 Fragments arranged by size: 1123 295 34 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 772 1489 1512 Size: 772 717 23 Fragments arranged by size: 772 717 23 BseYI C'CCAG_C Cuts at: 0 1269 1512 Size: 1269 243 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 13 577 1004 1512 Size: 13 564 427 508 Fragments arranged by size: 564 508 427 13 BsiHKAI G_wGCw'C Cuts at: 0 686 764 1499 1512 Size: 686 78 735 13 Fragments arranged by size: 735 686 78 13 Bsp1286I G_dGCh'C Cuts at: 0 136 647 686 713 764 1433 1499 1512 Size: 136 511 39 27 51 669 66 13 Fragments arranged by size: 669 511 136 66 51 39 27 13 BspHI T'CATG_A Cuts at: 0 660 1512 Size: 660 852 BstBI TT'CG_AA Cuts at: 0 1311 1512 Size: 1311 201 BstEII G'GTnAC_C Cuts at: 0 451 1512 Size: 451 1061 BstXI CCAn_nnnn'nTGG Cuts at: 0 113 1512 Size: 113 1399 BstYI r'GATC_y Cuts at: 0 938 1512 Size: 938 574 BtgI C'CryG_G Cuts at: 0 53 1359 1512 Size: 53 1306 153 Fragments arranged by size: 1306 153 53 BtsI GCAGTG_nn' Cuts at: 0 25 1139 1512 Size: 25 1114 373 Fragments arranged by size: 1114 373 25 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 491 526 1512 Size: 491 35 986 Fragments arranged by size: 986 491 35 DraIII CAC_nnn'GTG Cuts at: 0 1486 1512 Size: 1486 26 DrdI GACnn_nn'nnGTC Cuts at: 0 194 1512 Size: 194 1318 EarI CTCTTCn'nnn_ Cuts at: 0 12 70 1512 Size: 12 58 1442 Fragments arranged by size: 1442 58 12 EcoICRI GAG'CTC Cuts at: 0 684 762 1512 Size: 684 78 750 Fragments arranged by size: 750 684 78 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 294 830 935 1040 1302 1385 1512 Size: 294 536 105 105 262 83 127 Fragments arranged by size: 536 294 262 127 105 105 83 EcoO109I rG'GnC_Cy Cuts at: 0 805 1104 1512 Size: 805 299 408 Fragments arranged by size: 805 408 299 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 486 640 1437 1512 Size: 486 154 797 75 Fragments arranged by size: 797 486 154 75 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 32 64 981 1013 1231 1263 1512 Size: 32 32 917 32 218 32 249 Fragments arranged by size: 917 249 218 32 32 32 32 MfeI C'AATT_G Cuts at: 0 1250 1512 Size: 1250 262 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1103 1512 Size: 1103 409 MslI CAynn'nnrTG Cuts at: 0 52 111 160 355 665 1512 Size: 52 59 49 195 310 847 Fragments arranged by size: 847 310 195 59 52 49 MspA1I CmG'CkG Cuts at: 0 979 1512 Size: 979 533 NcoI C'CATG_G Cuts at: 0 53 1359 1512 Size: 53 1306 153 Fragments arranged by size: 1306 153 53 PflMI CCAn_nnn'nTGG Cuts at: 0 229 1512 Size: 229 1283 PfoI T'CCnGG_A Cuts at: 0 807 842 1512 Size: 807 35 670 Fragments arranged by size: 807 670 35 PpuMI rG'GwC_Cy Cuts at: 0 805 1104 1512 Size: 805 299 408 Fragments arranged by size: 805 408 299 PshAI GACnn'nnGTC Cuts at: 0 342 1512 Size: 342 1170 PstI C_TGCA'G Cuts at: 0 1158 1445 1512 Size: 1158 287 67 Fragments arranged by size: 1158 287 67 PvuII CAG'CTG Cuts at: 0 979 1512 Size: 979 533 SacI G_AGCT'C Cuts at: 0 686 764 1512 Size: 686 78 748 Fragments arranged by size: 748 686 78 ScaI AGT'ACT Cuts at: 0 610 1512 Size: 610 902 SfcI C'TryA_G Cuts at: 0 1154 1441 1512 Size: 1154 287 71 Fragments arranged by size: 1154 287 71 SmlI C'TyrA_G Cuts at: 0 10 298 1512 Size: 10 288 1214 Fragments arranged by size: 1214 288 10 StuI AGG'CCT Cuts at: 0 8 1512 Size: 8 1504 StyI C'CwwG_G Cuts at: 0 53 705 1107 1359 1512 Size: 53 652 402 252 153 Fragments arranged by size: 652 402 252 153 53 TatI w'GTAC_w Cuts at: 0 608 1512 Size: 608 904 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 359 391 720 752 824 856 1052 1084 Size: 359 32 329 32 72 32 196 32 Cuts at: 1084 1512 Size: 428 Fragments arranged by size: 428 359 329 196 72 32 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 361 1512 Size: 361 1151 XmnI GAAnn'nnTTC Cuts at: 0 923 1512 Size: 923 589 Enzymes that do cut: AclI AcuI AflII AleI AlwNI ApoI AvaI BaeI BanII BbsI BcgI BciVI BlpI Bme1580I BplI BpmI BpuEI BsaI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BstBI BstEII BstXI BstYI BtgI BtsI CspCI DraIII DrdI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I MfeI MmeI MslI MspA1I NcoI PflMI PfoI PpuMI PshAI PstI PvuII SacI ScaI SfcI SmlI StuI StyI TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BamHI BanI BbeI BbvCI BclI BfrBI BglI BglII BmgBI BmrI BmtI Bpu10I BsaAI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstZ17I Bsu36I BtgZI ClaI DraI EaeI EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI XhoI XmaI ZraI