Restriction map for
pFN21AE3222
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BclI BcnI
BglI BglII BlnI BsaAI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MluI MluNI MroI MscI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AclI 544 AACGTT AA^CGTT
AflII 10 CTTAAG C^TTAAG
AlwI 939 GATCC GGATC(4/5)
Asp700I 919 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1100 CTCGGG C^[CT]CG[GA]G
BbsI 413 GTCTTC GAAGAC(2/6)
BcgI 1092 GCATACATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 670 GGATAC GTATCC(6/5)
BfrI 10 CTTAAG C^TTAAG
BlpI 1087 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1087 GCTCAGC GC^T[ACGT]AGC
BpuAI 413 GTCTTC GAAGAC(2/6)
BsaI 61 GGTCTC GGTCTC(1/5)
BsmFI 22 GTCCC GGGAC(10/14)
BsoBI 1100 CTCGGG C^[CT]CG[GA]G
BspHI 660 TCATGA T^CATGA
BstBI 1310 TTCGAA TT^CGAA
BstEII 451 GGTTACC G^GT[ACGT]ACC
BstPI 451 GGTTACC G^GT[ACGT]ACC
BstXI 106 CCACCAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 938 AGATCC [GA]^GATC[CT]
CelII 1087 GCTCAGC GC^T[ACGT]AGC
CfoI 604 GCGC GCG^C
DpnII 939 GATC ^GATC
DraIII 1481 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 188 GACAAAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 451 GGTTACC G^GT[ACGT]ACC
HapII 1280 CCGG C^CGG
HhaI 604 GCGC GCG^C
HinP1I 604 GCGC G^CGC
HpaII 1280 CCGG C^CGG
MaeII 545 ACGT A^CGT
MboI 939 GATC ^GATC
MfeI 1250 CAATTG C^AATTG
MflI 938 AGATCC [GA]^GATC[CT]
MspA1I 977 CAGCTG C[AC]G^C[GT]G
MspI 1280 CCGG C^CGG
MunI 1250 CAATTG C^AATTG
NdeII 939 GATC ^GATC
NspV 1310 TTCGAA TT^CGAA
PflMI 223 CCACTAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 338 GACTAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 544 AACGTT AA^CGTT
PvuII 977 CAGCTG CAG^CTG
RcaI 660 TCATGA T^CATGA
Sau3AI 939 GATC ^GATC
ScaI 608 AGTACT AGT^ACT
SfuI 1310 TTCGAA TT^CGAA
StuI 6 AGGCCT AGG^CCT
Van91I 223 CCACTAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 354 CCAAATGCTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 938 AGATCC [GA]^GATC[CT]
XmnI 919 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 693 CCGC CCGC(-3/-1)
942 CCGC
AlwNI 889 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1403 CAGACCCTG
BsaBI 493 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
939 GATCCGCATC
BseRI 1498 CTCCTC GAGGAG(10/8)
757 GAGGAG
BsiYI 223 CCACTAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1077 CCTCTCTCTGG
BslI 223 CCACTAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1077 CCTCTCTCTGG
DraII 804 AGGTCCT [GA]G^G[ACGT]CC[CT]
1103 GGGTCCT
DsaI 53 CCATGG C^C[GA][CT]GG
1359 CCATGG
EarI 64 CTCTTC CTCTTC(1/4)
17 GAAGAG
EcoO109I 804 AGGTCCT [GA]G^G[ACGT]CC[CT]
1103 GGGTCCT
Ksp632I 64 CTCTTC CTCTTC(1/4)
17 GAAGAG
MamI 493 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
939 GATCCGCATC
NcoI 53 CCATGG C^CATGG
1359 CCATGG
PpuMI 804 AGGTCCT [GA]G^G[AT]CC[CT]
1103 GGGTCCT
PstI 1154 CTGCAG CTGCA^G
1441 CTGCAG
SacI 682 GAGCTC GAGCT^C
760 GAGCTC
SfcI 1154 CTGCAG C^T[GA][CT]AG
1441 CTGCAG
SmlI 10 CTTAAG C^T[CT][GA]AG
298 CTCAAG
TfiI 1299 GATTC G^A[AT]TC
1366 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AcsI AfaI AluI ApoI AspHI
AvaII BanII BbvI BfaI BmyI
BpmI BsaJI BsgI BsiHKAI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeIII MboII MnlI
MseI MslI MvaI MwoI NlaIII
NlaIV PleI RsaI Sau96I ScrFI
SfaNI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I