TRMT61B ORF clone pFN21AE3207

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Halotag ORF Clone pFN21AE3207

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Clone info.

Species

human

Clone Name

pFN21AE3207 Sequence (with vector) Restriction Map

Product ID

FHC20956

ORF Sequence

DNA sequence (1431 bp)
Protein sequence (477 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3207

Symbol

TRMT61B

Description

tRNA methyltransferase 61B

Original Clone ID

cp93207

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1431 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 477 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1768.1 1.9e-196 100.0 TRMT61B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR014816 174 467 PF08704 tRNA methyltransferase complex GCD14 subunit Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3207 Download

>KIEE3207  1431 bp
ATGCTAATGGCATGGTGCCGCGGTCCTGTCTTGCTGTGCCTGCGGCAGGGGCTCGGAACC
AATTCATTCCTGCACGGCCTGGGGCAGGAGCCCTTCGAGGGAGCTCGGTCACTGTGTTGC
AGGTCCTCGCCTAGAGACCTGCGAGATGGAGAAAGAGAGCACGAGGCGGCACAAAGGAAA
GCCCCAGGAGCAGAGTCTTGCCCATCTCTCCCTCTGAGCATCTCGGACATTGGGACTGGA
TGTCTTTCGTCACTGGAAAACCTCAGACTGCCGACGCTGCGGGAAGAGTCATCACCTCGA
GAGCTCGAGGACTCGAGCGGAGACCAGGGCCGGTGCGGTCCCACACACCAGGGATCCGAG
GATCCTTCGATGCTCTCGCAGGCCCAGTCCGCTACCGAGGTCGAAGAGCGTCACGTCTCC
CCTTCTTGTTCAACTTCCAGAGAGAGACCCTTTCAGGCTGGGGAGCTGATTTTAGCTGAG
ACTGGGGAGGGAGAAACAAAATTTAAGAAATTATTTAGGTTGAACAACTTCGGACTCTTA
AATAGTAACTGGGGGGCAGTCCCGTTCGGCAAGATCGTGGGGAAGTTCCCCGGCCAGATA
CTGAGGAGTTCCTTCGGTAAGCAGTACATGCTGAGGAGGCCAGCCTTGGAAGACTATGTA
GTATTGATGAAAAGAGGGACTGCCATAACATTCCCAAAGGATATTAATATGATTCTCTCA
ATGATGGATATCAACCCAGGTGATACTGTTTTGGAAGCTGGCTCAGGCTCTGGTGGAATG
AGCTTATTTTTATCCAAAGCAGTTGGATCACAAGGACGAGTCATAAGTTTTGAGGTACGA
AAAGACCACCATGATCTGGCTAAGAAGAATTACAAACACTGGCGTGATTCATGGAAATTA
AGTCATGTAGAAGAGTGGCCAGACAATGTGGATTTTATTCATAAGGACATTTCAGGAGCA
ACCGAAGACATAAAATCTTTAACATTTGACGCAGTAGCTTTGGATATGTTAAATCCTCAT
GTTACTTTGCCTGTTTTTTACCCACATCTTAAGCATGGTGGTGTATGTGCTGTATATGTA
GTAAACATCACACAGGTTATTGAACTTTTAGATGGAATTCGCACCTGTGAACTTGCTCTT
TCATGTGAAAAGATAAGCGAGGTCATTGTCAGAGATTGGTTGGTTTGCCTTGCAAAACAG
AAAAATGGAATTTTAGCTCAAAAAGTAGAATCTAAAATCAACACAGATGTACAACTAGAT
TCTCAAGAGAAAATTGGAGTTAAAGGTGAGCTGTTTCAAGAGGATGACCATGAAGAATCG
CATTCTGATTTTCCATATGGATCATTTCCCTATGTTGCTAGACCAGTACACTGGCAACCT
GGTCATACAGCTTTTCTTGTCAAGTTGAGGAAGGTCAAACCACAACTTAAC

ORF Protein

Cloned ORF protein sequence for pFN21AE3207 Download

>KIEE3207  477 aa
MLMAWCRGPVLLCLRQGLGTNSFLHGLGQEPFEGARSLCCRSSPRDLRDGEREHEAAQRK
APGAESCPSLPLSISDIGTGCLSSLENLRLPTLREESSPRELEDSSGDQGRCGPTHQGSE
DPSMLSQAQSATEVEERHVSPSCSTSRERPFQAGELILAETGEGETKFKKLFRLNNFGLL
NSNWGAVPFGKIVGKFPGQILRSSFGKQYMLRRPALEDYVVLMKRGTAITFPKDINMILS
MMDINPGDTVLEAGSGSGGMSLFLSKAVGSQGRVISFEVRKDHHDLAKKNYKHWRDSWKL
SHVEEWPDNVDFIHKDISGATEDIKSLTFDAVALDMLNPHVTLPVFYPHLKHGGVCAVYV
VNITQVIELLDGIRTCELALSCEKISEVIVRDWLVCLAKQKNGILAQKVESKINTDVQLD
SQEKIGVKGELFQEDDHEESHSDFPYGSFPYVARPVHWQPGHTAFLVKLRKVKPQLN

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3207 Download

>pFN21AE3207  6136 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCTAATGGCATGGTGCCGCGGTCCTGTCTTGCT
GTGCCTGCGGCAGGGGCTCGGAACCAATTCATTCCTGCACGGCCTGGGGCAGGAGCCCTT
CGAGGGAGCTCGGTCACTGTGTTGCAGGTCCTCGCCTAGAGACCTGCGAGATGGAGAAAG
AGAGCACGAGGCGGCACAAAGGAAAGCCCCAGGAGCAGAGTCTTGCCCATCTCTCCCTCT
GAGCATCTCGGACATTGGGACTGGATGTCTTTCGTCACTGGAAAACCTCAGACTGCCGAC
GCTGCGGGAAGAGTCATCACCTCGAGAGCTCGAGGACTCGAGCGGAGACCAGGGCCGGTG
CGGTCCCACACACCAGGGATCCGAGGATCCTTCGATGCTCTCGCAGGCCCAGTCCGCTAC
CGAGGTCGAAGAGCGTCACGTCTCCCCTTCTTGTTCAACTTCCAGAGAGAGACCCTTTCA
GGCTGGGGAGCTGATTTTAGCTGAGACTGGGGAGGGAGAAACAAAATTTAAGAAATTATT
TAGGTTGAACAACTTCGGACTCTTAAATAGTAACTGGGGGGCAGTCCCGTTCGGCAAGAT
CGTGGGGAAGTTCCCCGGCCAGATACTGAGGAGTTCCTTCGGTAAGCAGTACATGCTGAG
GAGGCCAGCCTTGGAAGACTATGTAGTATTGATGAAAAGAGGGACTGCCATAACATTCCC
AAAGGATATTAATATGATTCTCTCAATGATGGATATCAACCCAGGTGATACTGTTTTGGA
AGCTGGCTCAGGCTCTGGTGGAATGAGCTTATTTTTATCCAAAGCAGTTGGATCACAAGG
ACGAGTCATAAGTTTTGAGGTACGAAAAGACCACCATGATCTGGCTAAGAAGAATTACAA
ACACTGGCGTGATTCATGGAAATTAAGTCATGTAGAAGAGTGGCCAGACAATGTGGATTT
TATTCATAAGGACATTTCAGGAGCAACCGAAGACATAAAATCTTTAACATTTGACGCAGT
AGCTTTGGATATGTTAAATCCTCATGTTACTTTGCCTGTTTTTTACCCACATCTTAAGCA
TGGTGGTGTATGTGCTGTATATGTAGTAAACATCACACAGGTTATTGAACTTTTAGATGG
AATTCGCACCTGTGAACTTGCTCTTTCATGTGAAAAGATAAGCGAGGTCATTGTCAGAGA
TTGGTTGGTTTGCCTTGCAAAACAGAAAAATGGAATTTTAGCTCAAAAAGTAGAATCTAA
AATCAACACAGATGTACAACTAGATTCTCAAGAGAAAATTGGAGTTAAAGGTGAGCTGTT
TCAAGAGGATGACCATGAAGAATCGCATTCTGATTTTCCATATGGATCATTTCCCTATGT
TGCTAGACCAGTACACTGGCAACCTGGTCATACAGCTTTTCTTGTCAAGTTGAGGAAGGT
CAAACCACAACTTAACGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT