(Circular) (Six-base) MAPSORT of: pFN21AE3207.seq Check: 8804 from: 1 to: 6136 pFN21AE3207 6136 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3946 278 Size: 53 83 186 936 2410 2468 Fragments arranged by size: 2468 2410 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3479 1937 Size: 1542 4594 Acc65I G'GTAC_C Cuts at: 3457 3457 Size: 6136 AclI AA'CG_TT Cuts at: 1563 3435 4263 4636 1563 Size: 1872 828 373 3063 Fragments arranged by size: 3063 1872 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4199 5583 5851 1632 Size: 2567 1384 268 1917 Fragments arranged by size: 2567 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 3053 820 Size: 197 2036 3903 Fragments arranged by size: 3903 2036 197 AflIII A'CryG_T Cuts at: 1203 5035 6012 1203 Size: 3832 977 1327 Fragments arranged by size: 3832 1327 977 AgeI A'CCGG_T Cuts at: 4967 4967 Size: 6136 AhdI GACnn_n'nnGTC Cuts at: 2244 4865 2244 Size: 2621 3515 AleI CACnn'nnGTG Cuts at: 1166 2886 1166 Size: 1720 4416 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2218 1184 Size: 1034 5102 AlwNI CAG_nnn'CTG Cuts at: 2605 2774 3879 5451 2605 Size: 169 1105 1572 3290 Fragments arranged by size: 3290 1572 1105 169 ApaLI G'TGCA_C Cuts at: 4193 5349 4193 Size: 1156 4980 ApoI r'AATT_y Cuts at: 1493 2504 3120 3213 3445 3671 3823 5932 1493 Size: 1011 616 93 232 226 152 2109 1697 Fragments arranged by size: 2109 1697 1011 616 232 226 152 93 AseI AT'TA_AT Cuts at: 160 1032 2709 4690 160 Size: 872 1677 1981 1606 Fragments arranged by size: 1981 1677 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 AvaI C'yCGr_G Cuts at: 1870 1943 2301 2309 2317 3461 1870 Size: 73 358 8 8 1144 4545 Fragments arranged by size: 4545 1144 358 73 8 8 BamHI G'GATC_C Cuts at: 2357 2365 3466 3833 2357 Size: 8 1101 367 4660 Fragments arranged by size: 4660 1101 367 8 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2019 3457 4912 618 Size: 325 217 627 232 1438 1455 1842 Fragments arranged by size: 1842 1455 1438 627 325 232 217 BanII G_rGCy'C Cuts at: 1391 2058 2097 2110 2310 3455 1391 Size: 667 39 13 200 1145 4072 Fragments arranged by size: 4072 1145 667 200 39 13 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2661 2976 928 Size: 971 762 315 4088 Fragments arranged by size: 4088 971 762 315 BbvCI CC'TCA_GC Cuts at: 2636 2636 Size: 6136 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4327 4361 1903 Size: 34 50 34 2306 34 3678 Fragments arranged by size: 3678 2306 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4028 5238 4028 Size: 1210 4926 BclI T'GATC_A Cuts at: 1548 1548 Size: 6136 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4747 136 Size: 107 122 71 631 605 3075 1525 Fragments arranged by size: 3075 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6131 6131 Size: 6136 BlpI GC'TnA_GC Cuts at: 3553 3553 Size: 6136 Bme1580I G_kGCm'C Cuts at: 1790 4197 5353 1790 Size: 2407 1156 2573 Fragments arranged by size: 2573 2407 1156 BmgBI CAC'GTC Cuts at: 1318 2419 1318 Size: 1101 5035 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2383 2496 2563 Size: 416 53 83 399 24 966 113 67 Cuts at: 2563 3867 4825 442 Size: 1304 958 1753 Fragments arranged by size: 1753 1304 966 958 416 399 113 83 67 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6136 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4796 1239 Size: 3557 2579 Bpu10I CC'TnA_GC Cuts at: 2636 2767 2636 Size: 131 6005 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3252 4261 5126 5424 3252 Size: 1009 865 298 3964 Fragments arranged by size: 3964 1009 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2133 2319 2443 4799 882 Size: 617 634 186 124 2356 2219 Fragments arranged by size: 2356 2219 634 617 186 124 BsaAI yAC'GTr Cuts at: 493 1204 3927 493 Size: 711 2723 2702 Fragments arranged by size: 2723 2702 711 BsaBI GATnn'nnATC Cuts at: 2732 6130 2732 Size: 3398 2738 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3943 4325 4960 275 Size: 53 83 186 936 2410 382 635 1451 Fragments arranged by size: 2410 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4569 4967 5241 5388 5799 4569 Size: 398 274 147 411 4906 Fragments arranged by size: 4906 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5911 Fragments arranged by size: 5911 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2623 2653 1186 Size: 1437 30 4669 Fragments arranged by size: 4669 1437 30 BseYI C'CCAG_C Cuts at: 2462 5339 5835 2462 Size: 2877 496 2763 Fragments arranged by size: 2877 2763 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2060 1163 Size: 573 324 5239 Fragments arranged by size: 5239 573 324 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3586 4347 5375 1540 Size: 275 186 1585 761 1028 2301 Fragments arranged by size: 2301 1585 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2110 2166 2310 4197 4282 5353 2110 Size: 56 144 1887 85 1071 2893 Fragments arranged by size: 2893 1887 1071 144 85 56 BsmI GAATG_Cn' Cuts at: 3325 3647 3740 3325 Size: 322 93 5721 Fragments arranged by size: 5721 322 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2425 2425 Size: 6136 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2097 2110 2166 2310 3455 4197 Size: 399 268 39 13 56 144 1145 742 Cuts at: 4197 4282 5353 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1145 1071 742 399 268 144 85 56 39 13 BspEI T'CCGG_A Cuts at: 5799 5799 Size: 6136 BspHI T'CATG_A Cuts at: 4025 5755 4025 Size: 1730 4406 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2115 2151 3491 844 Size: 1271 36 1340 3489 Fragments arranged by size: 3489 1340 1271 36 BsrBI CCG'CTC Cuts at: 1394 1823 2322 4023 1394 Size: 429 499 1701 3507 Fragments arranged by size: 3507 1701 499 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4631 4805 65 Size: 1183 3383 174 1396 Fragments arranged by size: 3383 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2334 4780 4967 1700 Size: 110 524 2446 187 2869 Fragments arranged by size: 2869 2446 524 187 110 BsrGI T'GTAC_A Cuts at: 96 3253 96 Size: 3157 2979 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6136 BssSI C'ACGA_G Cuts at: 2165 4196 5208 2165 Size: 2031 1012 3093 Fragments arranged by size: 3093 2031 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2632 794 Size: 1838 4298 BstBI TT'CG_AA Cuts at: 4940 4940 Size: 6136 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6136 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3375 2011 Size: 1364 4772 BstYI r'GATC_y Cuts at: 1972 2357 2365 3466 3833 4218 4235 5687 5773 Size: 385 8 1101 367 385 17 1452 86 Cuts at: 5773 5785 6131 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1101 385 385 367 346 86 17 12 8 BtgI C'CryG_G Cuts at: 513 1065 2023 513 Size: 552 958 4626 Fragments arranged by size: 4626 958 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5397 Fragments arranged by size: 5397 683 56 BtsI GCAGTG_nn' Cuts at: 3652 4446 4466 3652 Size: 794 20 5322 Fragments arranged by size: 5322 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6136 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6101 DraI TTT'AAA Cuts at: 3440 3440 Size: 6136 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5143 809 Size: 507 3827 1802 Fragments arranged by size: 3827 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2596 2921 3583 Size: 54 1425 50 275 18 766 325 662 Cuts at: 3583 4472 6101 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 766 662 325 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3583 1537 Size: 275 1771 4090 Fragments arranged by size: 4090 1771 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2283 2403 2910 4066 1353 Size: 930 120 507 1156 3423 Fragments arranged by size: 3423 1156 930 507 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4723 5097 5243 387 Size: 1064 138 3134 374 146 1280 Fragments arranged by size: 3134 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2108 2308 2108 Size: 200 5936 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4199 4796 5583 5851 1239 Size: 393 2567 597 787 268 1524 Fragments arranged by size: 2567 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2048 2087 2048 Size: 39 6097 EcoO109I rG'GnC_Cy Cuts at: 2127 2127 Size: 6136 EcoRI G'AATT_C Cuts at: 3120 3445 3823 3120 Size: 325 378 5433 Fragments arranged by size: 5433 378 325 EcoRV GAT'ATC Cuts at: 2734 2734 Size: 6136 FspI TGC'GCA Cuts at: 4642 4642 Size: 6136 HaeII r_GCGC'y Cuts at: 1678 5283 1678 Size: 3605 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2230 2262 2963 2995 4777 Size: 32 135 32 658 32 701 32 1782 Cuts at: 4777 4809 4851 4883 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1782 701 658 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3480 3726 669 Size: 988 281 1542 246 3079 Fragments arranged by size: 3079 1542 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6136 HpaI GTT'AAC Cuts at: 3726 3726 Size: 6136 KpnI G_GTAC'C Cuts at: 3461 3461 Size: 6136 MfeI C'AATT_G Cuts at: 3735 3735 Size: 6136 MluI A'CGCG_T Cuts at: 6012 6012 Size: 6136 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2788 5250 5434 1305 Size: 1483 2462 184 2007 Fragments arranged by size: 2462 2007 1483 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2923 10 Size: 54 1425 343 1091 3223 Fragments arranged by size: 3223 1425 1091 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2886 3063 4094 4453 4612 518 Size: 648 843 877 177 1031 359 159 2042 Fragments arranged by size: 2042 1031 877 843 648 359 177 159 MspA1I CmG'CkG Cuts at: 1606 2025 3552 4229 5377 5622 1606 Size: 419 1527 677 1148 245 2120 Fragments arranged by size: 2120 1527 1148 677 419 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5584 NdeI CA'TA_TG Cuts at: 387 3340 387 Size: 2953 3183 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6136 NotI GC'GGCC_GC Cuts at: 3583 3583 Size: 6136 NspI r_CATG'y Cuts at: 1132 1229 2635 3494 5039 1132 Size: 97 1406 859 1545 2229 Fragments arranged by size: 2229 1545 1406 859 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6136 PciI A'CATG_T Cuts at: 5035 5035 Size: 6136 PflMI CCAn_nnn'nTGG Cuts at: 1108 2860 1108 Size: 1752 4384 PmeI GTTT'AAAC Cuts at: 3440 3440 Size: 6136 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4205 4237 5718 5750 4205 Size: 32 1481 32 4591 Fragments arranged by size: 4591 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2127 2127 Size: 6136 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6136 PsiI TTA'TAA Cuts at: 3706 3706 Size: 6136 PspXI vC'TCGA_Gb Cuts at: 2309 2317 2309 Size: 8 6128 PstI C_TGCA'G Cuts at: 830 3488 830 Size: 2658 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6136 SacI G_AGCT'C Cuts at: 2110 2310 2110 Size: 200 5936 SacII CC_GC'GG Cuts at: 2026 2026 Size: 6136 SalI G'TCGA_C Cuts at: 1936 3478 1936 Size: 1542 4594 SapI GCTCTTCn'nnn_ Cuts at: 2403 2403 Size: 6136 SbfI CC_TGCA'GG Cuts at: 3488 3488 Size: 6136 ScaI AGT'ACT Cuts at: 4384 6096 4384 Size: 1712 4424 SexAI A'CCwGG_T Cuts at: 3382 3382 Size: 6136 SfcI C'TryA_G Cuts at: 826 1045 3484 4619 5300 5491 826 Size: 219 2439 1135 681 191 1471 Fragments arranged by size: 2439 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6136 SmaI CCC'GGG Cuts at: 1872 3463 1872 Size: 1591 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2301 2309 2317 3053 3267 4240 Size: 197 926 358 8 8 736 214 973 Cuts at: 4240 5141 5403 820 Size: 901 262 1553 Fragments arranged by size: 1553 973 926 901 736 358 262 214 197 8 8 SnaBI TAC'GTA Cuts at: 493 3927 493 Size: 3434 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6136 SphI G_CATG'C Cuts at: 1132 3494 1132 Size: 2362 3774 SspI AAT'ATT Cuts at: 5 52 4060 5 Size: 47 4008 2081 Fragments arranged by size: 4008 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6136 StyI C'CwwG_G Cuts at: 513 1065 2649 3575 513 Size: 552 1584 926 3074 Fragments arranged by size: 3074 1584 926 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2100 4163 4180 4333 4518 4963 325 Size: 1255 520 2063 17 153 185 445 1498 Fragments arranged by size: 2063 1498 1255 520 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2628 3253 3370 Size: 274 80 33 51 1443 651 625 117 Cuts at: 3370 4382 6094 96 Size: 1012 1712 138 Fragments arranged by size: 1712 1443 1012 651 625 274 138 117 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2342 2374 6096 6128 2342 Size: 32 3722 32 2350 Fragments arranged by size: 3722 2350 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6136 XbaI T'CTAG_A Cuts at: 3472 3472 Size: 6136 XhoI C'TCGA_G Cuts at: 1943 2301 2309 2317 1943 Size: 358 8 8 5762 Fragments arranged by size: 5762 358 8 8 XmaI C'CCGG_G Cuts at: 1870 3461 1870 Size: 1591 4545 XmnI GAAnn'nnTTC Cuts at: 2065 2531 4265 2065 Size: 466 1734 3936 Fragments arranged by size: 3936 1734 466 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3944 276 Size: 53 83 186 936 2410 2468 Fragments arranged by size: 2468 2410 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI PvuII RsrII SanDI SfiI SfoI SrfI SwaI XcmI