(Linear) (Six-base) MAPSORT of: KIEE3207.seq Check: 3177 from: 1 to: 1431 KIEE3207.orf With 182 enzymes: SgfI * July 3, 2012 17:26 .. AflII C'TTAA_G Cuts at: 0 1048 1431 Size: 1048 383 AhdI GACnn_n'nnGTC Cuts at: 0 239 1431 Size: 239 1192 AleI CACnn'nnGTG Cuts at: 0 881 1431 Size: 881 550 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 213 1431 Size: 213 1218 AlwNI CAG_nnn'CTG Cuts at: 0 600 769 1431 Size: 600 169 662 Fragments arranged by size: 662 600 169 ApoI r'AATT_y Cuts at: 0 499 1115 1208 1431 Size: 499 616 93 223 Fragments arranged by size: 616 499 223 93 AseI AT'TA_AT Cuts at: 0 704 1431 Size: 704 727 AvaI C'yCGr_G Cuts at: 0 296 304 312 1431 Size: 296 8 8 1119 Fragments arranged by size: 1119 296 8 8 BamHI G'GATC_C Cuts at: 0 352 360 1431 Size: 352 8 1071 Fragments arranged by size: 1071 352 8 BanI G'GyrC_C Cuts at: 0 14 1431 Size: 14 1417 BanII G_rGCy'C Cuts at: 0 53 92 105 305 1431 Size: 53 39 13 200 1126 Fragments arranged by size: 1126 200 53 39 13 BbsI GAAGACnn'nnnn_ Cuts at: 0 656 971 1431 Size: 656 315 460 Fragments arranged by size: 656 460 315 BbvCI CC'TCA_GC Cuts at: 0 631 1431 Size: 631 800 BmgBI CAC'GTC Cuts at: 0 414 1431 Size: 414 1017 BmrI ACTGGGnnnn_n' Cuts at: 0 378 491 558 1431 Size: 378 113 67 873 Fragments arranged by size: 873 378 113 67 Bpu10I CC'TnA_GC Cuts at: 0 631 762 1431 Size: 631 131 669 Fragments arranged by size: 669 631 131 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1247 1431 Size: 1247 184 BsaI GGTCTCn'nnnn_ Cuts at: 0 128 314 438 1431 Size: 128 186 124 993 Fragments arranged by size: 993 186 128 124 BsaBI GATnn'nnATC Cuts at: 0 727 1431 Size: 727 704 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 618 648 1431 Size: 618 30 783 Fragments arranged by size: 783 618 30 BseYI C'CCAG_C Cuts at: 0 457 1431 Size: 457 974 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 55 1431 Size: 55 1376 BsiHKAI G_wGCw'C Cuts at: 0 105 161 305 1431 Size: 105 56 144 1126 Fragments arranged by size: 1126 144 105 56 BsmI GAATG_Cn' Cuts at: 0 1320 1431 Size: 1320 111 BsmBI CGTCTCn'nnnn_ Cuts at: 0 420 1431 Size: 420 1011 Bsp1286I G_dGCh'C Cuts at: 0 53 92 105 161 305 1431 Size: 53 39 13 56 144 1126 Fragments arranged by size: 1126 144 56 53 39 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 110 146 1431 Size: 110 36 1285 Fragments arranged by size: 1285 110 36 BsrBI CCG'CTC Cuts at: 0 317 1431 Size: 317 1114 BsrFI r'CCGG_y Cuts at: 0 329 1431 Size: 329 1102 BsrGI T'GTAC_A Cuts at: 0 1248 1431 Size: 1248 183 BssSI C'ACGA_G Cuts at: 0 160 1431 Size: 160 1271 BstAPI GCAn_nnn'nTGC Cuts at: 0 627 1431 Size: 627 804 BstXI CCAn_nnnn'nTGG Cuts at: 0 1370 1431 Size: 1370 61 BstYI r'GATC_y Cuts at: 0 352 360 1431 Size: 352 8 1071 Fragments arranged by size: 1071 352 8 BtgI C'CryG_G Cuts at: 0 18 1431 Size: 18 1413 EaeI y'GGCC_r Cuts at: 0 591 916 1431 Size: 591 325 515 Fragments arranged by size: 591 515 325 EarI CTCTTCn'nnn_ Cuts at: 0 278 398 905 1431 Size: 278 120 507 526 Fragments arranged by size: 526 507 278 120 EcoICRI GAG'CTC Cuts at: 0 103 303 1431 Size: 103 200 1128 Fragments arranged by size: 1128 200 103 EcoNI CCTnn'n_nnAGG Cuts at: 0 43 82 1431 Size: 43 39 1349 Fragments arranged by size: 1349 43 39 EcoO109I rG'GnC_Cy Cuts at: 0 122 1431 Size: 122 1309 EcoRI G'AATT_C Cuts at: 0 1115 1431 Size: 1115 316 EcoRV GAT'ATC Cuts at: 0 729 1431 Size: 729 702 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 225 257 958 990 1431 Size: 225 32 701 32 441 Fragments arranged by size: 701 441 225 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 783 1431 Size: 783 648 MscI TGG'CCA Cuts at: 0 918 1431 Size: 918 513 MslI CAynn'nnrTG Cuts at: 0 881 1058 1431 Size: 881 177 373 Fragments arranged by size: 881 373 177 MspA1I CmG'CkG Cuts at: 0 20 1431 Size: 20 1411 NdeI CA'TA_TG Cuts at: 0 1335 1431 Size: 1335 96 NspI r_CATG'y Cuts at: 0 630 1431 Size: 630 801 PflMI CCAn_nnn'nTGG Cuts at: 0 855 1431 Size: 855 576 PpuMI rG'GwC_Cy Cuts at: 0 122 1431 Size: 122 1309 PspXI vC'TCGA_Gb Cuts at: 0 304 312 1431 Size: 304 8 1119 Fragments arranged by size: 1119 304 8 SacI G_AGCT'C Cuts at: 0 105 305 1431 Size: 105 200 1126 Fragments arranged by size: 1126 200 105 SacII CC_GC'GG Cuts at: 0 21 1431 Size: 21 1410 SapI GCTCTTCn'nnn_ Cuts at: 0 398 1431 Size: 398 1033 SexAI A'CCwGG_T Cuts at: 0 1377 1431 Size: 1377 54 SmlI C'TyrA_G Cuts at: 0 296 304 312 1048 1262 1431 Size: 296 8 8 736 214 169 Fragments arranged by size: 736 296 214 169 8 8 StyI C'CwwG_G Cuts at: 0 644 1431 Size: 644 787 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 95 1431 Size: 95 1336 TatI w'GTAC_w Cuts at: 0 623 1248 1365 1431 Size: 623 625 117 66 Fragments arranged by size: 625 623 117 66 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 337 369 1431 Size: 337 32 1062 Fragments arranged by size: 1062 337 32 XhoI C'TCGA_G Cuts at: 0 296 304 312 1431 Size: 296 8 8 1119 Fragments arranged by size: 1119 296 8 8 XmnI GAAnn'nnTTC Cuts at: 0 60 526 1431 Size: 60 466 905 Fragments arranged by size: 905 466 60 Enzymes that do cut: AflII AhdI AleI AlfI AlwNI ApoI AseI AvaI BamHI BanI BanII BbsI BbvCI BmgBI BmrI Bpu10I BpuEI BsaI BsaBI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstXI BstYI BtgI EaeI EarI EcoICRI EcoNI EcoO109I EcoRI EcoRV Hin4I MmeI MscI MslI MspA1I NdeI NspI PflMI PpuMI PspXI SacI SacII SapI SexAI SmlI StyI TaqII TatI TstI XhoI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmtI BplI BpmI BsaAI BsaHI BsaWI BsaXI BsiEI BsiWI BspEI BspHI BsrDI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI Eco57MI FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XmaI ZraI