################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspI AviII AvrII BbeI BbrPI BcgI BciVI BclI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspHI BspLU11I BsrDI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI MfeI MluI MroI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SalI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccII 19 CGCG CG^CG AflII 1048 CTTAAG C^TTAAG AhdI 234 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 703 ATTAAT AT^TAAT AsnI 703 ATTAAT AT^TAAT AspEI 234 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 916 TGGCCA TGG^CCA BanI 14 GGTGCC G^G[CT][GA]CC BbvCI 630 GCTGAGG CCTCAGC(-5/-2) BbvI 276 GCTGC GCAGC(8/12) BcnI 589 CCCGG CC^[GC]GG BfrI 1048 CTTAAG C^TTAAG BsaBI 723 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 70 CTGCAC GTGCAG(16/14) BsmBI 414 CGTCTC CGTCTC(1/5) BsmI 1320 GCATTC GAATGC(1/-1) Bsp1407I 1248 TGTACA T^GTACA BsrBI 315 GAGCGG CCGCTC(-3/-3) BsrFI 329 GCCGGT [GA]^CCGG[CT] BsrGI 1248 TGTACA T^GTACA BssSI 160 CACGAG CACGAG(-5/-1) BstAPI 621 GCAGTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 19 CGCG CG^CG BstXI 1363 CCAGTACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 329 GCCGGT [GA]^CCGG[CT] DraII 121 AGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 18 CCGCGG C^C[GA][CT]GG Eam1105I 234 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 121 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 1115 GAATTC G^AATTC EcoRV 727 GATATC GAT^ATC EcoT14I 644 CCTTGG C^C[AT][AT]GG FauI 279 GCGGG CCCGC(4/6) KspI 18 CCGCGG CCGC^GG MaeII 413 ACGT A^CGT MamI 723 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 916 TGGCCA TGG^CCA MscI 916 TGGCCA TGG^CCA MspA1I 18 CCGCGG C[AC]G^C[GT]G MvnI 19 CGCG CG^CG NciI 589 CCCGG CC^[GC]GG NdeI 1334 CATATG CA^TATG NspI 626 ACATGC [GA]CATG^[CT] PflMI 849 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 121 AGGTCCT [GA]G^G[AT]CC[CT] PshBI 703 ATTAAT AT^TAAT SacII 18 CCGCGG CCGC^GG SapI 403 GAAGAGC GCTCTTC(1/4) SexAI 1377 ACCTGGT A^CC[AT]GGT SspBI 1248 TGTACA T^GTACA StyI 644 CCTTGG C^C[AT][AT]GG TseI 276 GCTGC G^C[AT]GC Van91I 849 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwNI 595 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG 764 CAGGCTCTG Asp700I 56 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 522 GAACAACTTC BamHI 352 GGATCC G^GATCC 360 GGATCC BbsI 649 GAAGAC GAAGAC(2/6) 964 GAAGAC BpuAI 649 GAAGAC GAAGAC(2/6) 964 GAAGAC BseRI 603 GAGGAG GAGGAG(10/8) 633 GAGGAG BspMI 137 ACCTGC ACCTGC(4/8) 119 GCAGGT BstF5I 238 GGATG GGATG(2/0) 1302 GGATG BstYI 352 GGATCC [GA]^GATC[CT] 360 GGATCC EaeI 591 CGGCCA [CT]^GGCC[GA] 916 TGGCCA EcoNI 39 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 78 CCTGGGGCAGG FokI 238 GGATG GGATG(9/13) 1302 GGATG HapII 330 CCGG C^CGG 590 CCGG HpaII 330 CCGG C^CGG 590 CCGG MflI 352 GGATCC [GA]^GATC[CT] 360 GGATCC MslI 877 CACTGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1054 CATGGTGGTG MspI 330 CCGG C^CGG 590 CCGG SacI 101 GAGCTC GAGCT^C 301 GAGCTC SfaNI 369 GATGC GCATC(5/9) 218 GCATC XhoII 352 GGATCC [GA]^GATC[CT] 360 GGATCC XmnI 56 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 522 GAACAACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaI AvaII BanII BfaI BmyI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI HaeIII HgaI HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PaeR7I PleI RsaI Sau3AI Sau96I ScrFI SmlI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI TthHB8I XhoI