NARFL ORF clone pFN21AE3205

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE3205

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Clone info.

Species

human

Clone Name

pFN21AE3205 Sequence (with vector) Restriction Map

Product ID

FHC25536

ORF Sequence

DNA sequence (1428 bp)
Protein sequence (476 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3205

Symbol

NARFL

Description

nuclear prelamin A recognition factor-like, transcript variant 1

Original Clone ID

cp93205

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1428 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 476 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10425.1 4.3e-208 100.0 NARFL CCDS76798.1 7.7e-164 100.0 NARFL CCDS32777.1 4e-91 48.1 NARF CCDS42404.1 5.6e-85 50.2 NARF CCDS42403.1 1.2e-81 48.4 NARF The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR004108 105 406 PF02906 Iron hydrogenase IPR003149 415 465 PF02256 Iron hydrogenase HMMSmart IPR003149 410 466 SM00902 Iron hydrogenase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_071938 476 611118 100.0 100.0 NP_036468 456 605349 48.1 95.6 XP_011521843 397 605349 50.2 83.8 XP_005256397 397 605349 50.2 83.8 NP_001033707 397 605349 50.2 83.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3205 Download

>KIEE3205  1428 bp
ATGGCGTCGCCCTTCAGCGGGGCGCTGCAGCTGACGGACCTGGATGACTTCATCGGGCCG
TCTCAGGAGTGCATCAAGCCTGTCAAAGTGGAAAAAAGGGCGGGAAGTGGCGTGGCCAAG
ATTCGCATTGAAGATGACGGGAGCTACTTCCAAATTAACCAAGACGGCGGGACCCGGAGG
CTGGAGAAGGCCAAGGTCTCGCTAAACGACTGCCTGGCGTGCAGCGGCTGCATCACCTCC
GCAGAGACCGTGCTTATCACCCAGCAGAGCCACGAGGAGCTGAAGAAGGTTCTAGATGCT
AACAAGATGGCGGCACCCAGTCAGCAGAGGCTGGTTGTAGTTTCGGTCTCACCACAGTCT
AGAGCATCGCTGGCTGCACGGTTTCAGCTGAATCCTACAGATACTGCCAGGAAATTAACC
TCATTCTTTAAAAAAATAGGGGTGCACTTCGTCTTCGACACCGCCTTCTCAAGGCACTTC
AGCCTCCTGGAGAGCCAGCGAGAGTTTGTGCGGCGATTCCGAGGACAGGCCGACTGCAGA
CAGGCGCTGCCCCTGCTGGCCTCTGCCTGCCCAGGCTGGATCTGCTATGCCGAGAAGACT
CACGGCAGCTTCATCCTCCCCCACATCAGCACCGCCCGGTCCCCGCAGCAGGTCATGGGC
TCCCTGGTCAAGGACTTCTTCGCCCAGCAGCAGCACTTGACCCCTGACAAGATCTACCAC
GTCACAGTGATGCCCTGCTATGACAAAAAGCTGGAAGCCTCCAGACCCGACTTTTTCAAC
CAGGAGCACCAGACACGGGATGTGGACTGTGTCCTCACAACAGGAGAAGTTTTCAGGTTG
CTGGAGGAAGAGGGCGTCTCCCTCCCCGACCTGGAACCAGCCCCTCTGGACAGCCTGTGC
AGCGGTGCCTCTGCAGAGGAGCCCACCAGCCATCGGGGAGGGGGCTCGGGGGGCTACCTG
GAGCACGTGTTCCGGCACGCGGCCCGAGAGCTCTTTGGAATCCATGTGGCTGAGGTTACC
TACAAACCCCTGAGGAACAAAGACTTCCAGGAGGTGACACTGGAGAAGGAGGGCCAGGTG
CTGCTGCACTTCGCAATGGCGTACGGCTTCCGCAACATCCAGAACCTGGTGCAGAGGCTC
AAACGAGGGCGCTGCCCCTACCACTACGTGGAGGTCATGGCCTGCCCCTCAGGCTGCCTG
AACGGCGGGGGCCAGCTCCAGGCCCCAGACAGGCCCAGCAGAGAGCTCCTCCAGCACGTG
GAGAGACTGTACGGCATGGTCCGGGCTGAGGCGCCCGAGGACGCGCCTGGGGTTCAGGAG
CTGTACACACACTGGCTGCAGGGCACGGACTCGGAGTGTGCAGGTCGCTTGCTGCATACG
CAGTACCACGCCGTGGAGAAGGCCAGCACTGGCCTGGGCATCCGGTGG

ORF Protein

Cloned ORF protein sequence for pFN21AE3205 Download

>KIEE3205  476 aa
MASPFSGALQLTDLDDFIGPSQECIKPVKVEKRAGSGVAKIRIEDDGSYFQINQDGGTRR
LEKAKVSLNDCLACSGCITSAETVLITQQSHEELKKVLDANKMAAPSQQRLVVVSVSPQS
RASLAARFQLNPTDTARKLTSFFKKIGVHFVFDTAFSRHFSLLESQREFVRRFRGQADCR
QALPLLASACPGWICYAEKTHGSFILPHISTARSPQQVMGSLVKDFFAQQQHLTPDKIYH
VTVMPCYDKKLEASRPDFFNQEHQTRDVDCVLTTGEVFRLLEEEGVSLPDLEPAPLDSLC
SGASAEEPTSHRGGGSGGYLEHVFRHAARELFGIHVAEVTYKPLRNKDFQEVTLEKEGQV
LLHFAMAYGFRNIQNLVQRLKRGRCPYHYVEVMACPSGCLNGGGQLQAPDRPSRELLQHV
ERLYGMVRAEAPEDAPGVQELYTHWLQGTDSECAGRLLHTQYHAVEKASTGLGIRW

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3205 Download

>pFN21AE3205  6133 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGTCGCCCTTCAGCGGGGCGCTGCAGCTGAC
GGACCTGGATGACTTCATCGGGCCGTCTCAGGAGTGCATCAAGCCTGTCAAAGTGGAAAA
AAGGGCGGGAAGTGGCGTGGCCAAGATTCGCATTGAAGATGACGGGAGCTACTTCCAAAT
TAACCAAGACGGCGGGACCCGGAGGCTGGAGAAGGCCAAGGTCTCGCTAAACGACTGCCT
GGCGTGCAGCGGCTGCATCACCTCCGCAGAGACCGTGCTTATCACCCAGCAGAGCCACGA
GGAGCTGAAGAAGGTTCTAGATGCTAACAAGATGGCGGCACCCAGTCAGCAGAGGCTGGT
TGTAGTTTCGGTCTCACCACAGTCTAGAGCATCGCTGGCTGCACGGTTTCAGCTGAATCC
TACAGATACTGCCAGGAAATTAACCTCATTCTTTAAAAAAATAGGGGTGCACTTCGTCTT
CGACACCGCCTTCTCAAGGCACTTCAGCCTCCTGGAGAGCCAGCGAGAGTTTGTGCGGCG
ATTCCGAGGACAGGCCGACTGCAGACAGGCGCTGCCCCTGCTGGCCTCTGCCTGCCCAGG
CTGGATCTGCTATGCCGAGAAGACTCACGGCAGCTTCATCCTCCCCCACATCAGCACCGC
CCGGTCCCCGCAGCAGGTCATGGGCTCCCTGGTCAAGGACTTCTTCGCCCAGCAGCAGCA
CTTGACCCCTGACAAGATCTACCACGTCACAGTGATGCCCTGCTATGACAAAAAGCTGGA
AGCCTCCAGACCCGACTTTTTCAACCAGGAGCACCAGACACGGGATGTGGACTGTGTCCT
CACAACAGGAGAAGTTTTCAGGTTGCTGGAGGAAGAGGGCGTCTCCCTCCCCGACCTGGA
ACCAGCCCCTCTGGACAGCCTGTGCAGCGGTGCCTCTGCAGAGGAGCCCACCAGCCATCG
GGGAGGGGGCTCGGGGGGCTACCTGGAGCACGTGTTCCGGCACGCGGCCCGAGAGCTCTT
TGGAATCCATGTGGCTGAGGTTACCTACAAACCCCTGAGGAACAAAGACTTCCAGGAGGT
GACACTGGAGAAGGAGGGCCAGGTGCTGCTGCACTTCGCAATGGCGTACGGCTTCCGCAA
CATCCAGAACCTGGTGCAGAGGCTCAAACGAGGGCGCTGCCCCTACCACTACGTGGAGGT
CATGGCCTGCCCCTCAGGCTGCCTGAACGGCGGGGGCCAGCTCCAGGCCCCAGACAGGCC
CAGCAGAGAGCTCCTCCAGCACGTGGAGAGACTGTACGGCATGGTCCGGGCTGAGGCGCC
CGAGGACGCGCCTGGGGTTCAGGAGCTGTACACACACTGGCTGCAGGGCACGGACTCGGA
GTGTGCAGGTCGCTTGCTGCATACGCAGTACCACGCCGTGGAGAAGGCCAGCACTGGCCT
GGGCATCCGGTGGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT