(Linear) (Six-base) MAPSORT of: KIEE3205.seq Check: 373 from: 1 to: 1428 KIEE3205.orf With 182 enzymes: SgfI * March 22, 2012 16:21 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 301 462 1428 Size: 301 161 966 Fragments arranged by size: 966 301 161 AflIII A'CryG_T Cuts at: 0 965 1428 Size: 965 463 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 952 984 1428 Size: 952 32 444 Fragments arranged by size: 952 444 32 AlwNI CAG_nnn'CTG Cuts at: 0 227 330 403 546 1080 1125 1320 1428 Size: 227 103 73 143 534 45 195 108 Fragments arranged by size: 534 227 195 143 108 103 73 45 ApaLI G'TGCA_C Cuts at: 0 442 1428 Size: 442 986 AvaI C'yCGr_G Cuts at: 0 945 983 1294 1428 Size: 945 38 311 134 Fragments arranged by size: 945 311 134 38 BanI G'GyrC_C Cuts at: 0 312 904 1290 1428 Size: 312 592 386 138 Fragments arranged by size: 592 386 312 138 BanII G_rGCy'C Cuts at: 0 661 923 946 992 1247 1428 Size: 661 262 23 46 255 181 Fragments arranged by size: 661 262 255 181 46 23 BbeI G_GCGC'C Cuts at: 0 1294 1428 Size: 1294 134 BbsI GAAGACnn'nnnn_ Cuts at: 0 444 601 1428 Size: 444 157 827 Fragments arranged by size: 827 444 157 BbvCI CC'TCA_GC Cuts at: 0 1010 1286 1428 Size: 1010 276 142 Fragments arranged by size: 1010 276 142 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1133 1167 1428 Size: 1133 34 261 Fragments arranged by size: 1133 261 34 BglI GCCn_nnn'nGGC Cuts at: 0 1190 1202 1428 Size: 1190 12 226 Fragments arranged by size: 1190 226 12 BglII A'GATC_T Cuts at: 0 710 1428 Size: 710 718 Bme1580I G_kGCm'C Cuts at: 0 446 1345 1428 Size: 446 899 83 Fragments arranged by size: 899 446 83 BmgBI CAC'GTC Cuts at: 0 720 1428 Size: 720 708 BmrI ACTGGGnnnn_n' Cuts at: 0 311 1428 Size: 311 1117 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 873 1264 1428 Size: 873 391 164 Fragments arranged by size: 873 391 164 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 508 744 862 979 1081 1201 1234 Size: 202 306 236 118 117 102 120 33 Cuts at: 1234 1428 Size: 194 Fragments arranged by size: 306 236 202 194 120 118 117 102 33 Bpu10I CC'TnA_GC Cuts at: 0 1010 1286 1428 Size: 1010 276 142 Fragments arranged by size: 1010 276 142 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 453 1428 Size: 453 975 BsaI GGTCTCn'nnnn_ Cuts at: 0 201 238 351 1428 Size: 201 37 113 1077 Fragments arranged by size: 1077 201 113 37 BsaAI yAC'GTr Cuts at: 0 966 1167 1257 1428 Size: 966 201 90 171 Fragments arranged by size: 966 201 171 90 BsaHI Gr'CG_yC Cuts at: 0 4 854 1291 1428 Size: 4 850 437 137 Fragments arranged by size: 850 437 137 4 BsaWI w'CCGG_w Cuts at: 0 1421 1428 Size: 1421 7 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 952 982 1252 1282 1428 Size: 952 30 270 30 146 Fragments arranged by size: 952 270 146 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 289 931 1237 1428 Size: 289 642 306 191 Fragments arranged by size: 642 306 289 191 BseYI C'CCAG_C Cuts at: 0 260 683 1234 1428 Size: 260 423 551 194 Fragments arranged by size: 551 423 260 194 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 240 359 918 1069 1150 1379 1428 Size: 240 119 559 151 81 229 49 Fragments arranged by size: 559 240 229 151 119 81 49 BsiHKAI G_wGCw'C Cuts at: 0 446 788 965 992 1247 1428 Size: 446 342 177 27 255 181 Fragments arranged by size: 446 342 255 181 177 27 BsiWI C'GTAC_G Cuts at: 0 1100 1428 Size: 1100 328 BsmBI CGTCTCn'nnnn_ Cuts at: 0 65 861 1428 Size: 65 796 567 Fragments arranged by size: 796 567 65 Bsp1286I G_dGCh'C Cuts at: 0 446 661 788 923 946 965 992 1247 Size: 446 215 127 135 23 19 27 255 Cuts at: 1247 1345 1428 Size: 98 83 Fragments arranged by size: 446 255 215 135 127 98 83 27 23 19 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 639 1351 1428 Size: 639 712 77 Fragments arranged by size: 712 639 77 BsrDI GCAATG_nn' Cuts at: 0 1100 1428 Size: 1100 328 BsrGI T'GTAC_A Cuts at: 0 1322 1428 Size: 1322 106 BssSI C'ACGA_G Cuts at: 0 271 1428 Size: 271 1157 BstAPI GCAn_nnn'nTGC Cuts at: 0 227 1428 Size: 227 1201 BstEII G'GTnAC_C Cuts at: 0 1014 1428 Size: 1014 414 BstYI r'GATC_y Cuts at: 0 578 710 1428 Size: 578 132 718 Fragments arranged by size: 718 578 132 Bsu36I CC'TnA_GG Cuts at: 0 1030 1188 1428 Size: 1030 158 240 Fragments arranged by size: 1030 240 158 BtgI C'CryG_G Cuts at: 0 1391 1428 Size: 1391 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 350 1428 Size: 350 1078 DraI TTT'AAA Cuts at: 0 429 1428 Size: 429 999 DraIII CAC_nnn'GTG Cuts at: 0 1167 1392 1428 Size: 1167 225 36 Fragments arranged by size: 1167 225 36 EaeI y'GGCC_r Cuts at: 0 113 1428 Size: 113 1315 EarI CTCTTCn'nnn_ Cuts at: 0 842 1428 Size: 842 586 EcoICRI GAG'CTC Cuts at: 0 990 1245 1428 Size: 990 255 183 Fragments arranged by size: 990 255 183 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 301 462 508 744 862 979 1081 Size: 202 99 161 46 236 118 117 102 Cuts at: 1081 1201 1234 1428 Size: 120 33 194 Fragments arranged by size: 236 202 194 161 120 118 117 102 99 46 33 EcoNI CCTnn'n_nnAGG Cuts at: 0 468 667 1428 Size: 468 199 761 Fragments arranged by size: 761 468 199 EcoO109I rG'GnC_Cy Cuts at: 0 170 1221 1428 Size: 170 1051 207 Fragments arranged by size: 1051 207 170 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 693 1428 Size: 693 735 HaeII r_GCGC'y Cuts at: 0 25 547 1152 1294 1428 Size: 25 522 605 142 134 Fragments arranged by size: 605 522 142 134 25 KasI G'GCGC_C Cuts at: 0 1290 1428 Size: 1290 138 MscI TGG'CCA Cuts at: 0 115 1428 Size: 115 1313 MslI CAynn'nnrTG Cuts at: 0 727 1428 Size: 727 701 MspA1I CmG'CkG Cuts at: 0 17 30 224 387 902 1428 Size: 17 13 194 163 515 526 Fragments arranged by size: 526 515 194 163 17 13 NarI GG'CG_CC Cuts at: 0 1291 1428 Size: 1291 137 PflMI CCAn_nnn'nTGG Cuts at: 0 1125 1167 1257 1392 1428 Size: 1125 42 90 135 36 Fragments arranged by size: 1125 135 90 42 36 PfoI T'CCnGG_A Cuts at: 0 485 1046 1428 Size: 485 561 382 Fragments arranged by size: 561 485 382 PmlI CAC'GTG Cuts at: 0 966 1257 1428 Size: 966 291 171 Fragments arranged by size: 966 291 171 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 952 984 1428 Size: 952 32 444 Fragments arranged by size: 952 444 32 PpuMI rG'GwC_Cy Cuts at: 0 170 1428 Size: 170 1258 PstI C_TGCA'G Cuts at: 0 29 538 915 1340 1428 Size: 29 509 377 425 88 Fragments arranged by size: 509 425 377 88 29 PvuII CAG'CTG Cuts at: 0 30 387 1428 Size: 30 357 1041 Fragments arranged by size: 1041 357 30 SacI G_AGCT'C Cuts at: 0 992 1247 1428 Size: 992 255 181 Fragments arranged by size: 992 255 181 SanDI GG'GwC_CC Cuts at: 0 170 1428 Size: 170 1258 SexAI A'CCwGG_T Cuts at: 0 1124 1428 Size: 1124 304 SfcI C'TryA_G Cuts at: 0 25 395 534 911 1336 1428 Size: 25 370 139 377 425 92 Fragments arranged by size: 425 377 370 139 92 25 SfoI GGC'GCC Cuts at: 0 1292 1428 Size: 1292 136 SmlI C'TyrA_G Cuts at: 0 468 1428 Size: 468 960 StyI C'CwwG_G Cuts at: 0 191 1428 Size: 191 1237 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 274 330 333 1428 Size: 274 56 3 1095 Fragments arranged by size: 1095 274 56 3 TatI w'GTAC_w Cuts at: 0 1322 1428 Size: 1322 106 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 95 127 880 912 989 1021 1339 1371 Size: 95 32 753 32 77 32 318 32 Cuts at: 1371 1428 Size: 57 Fragments arranged by size: 753 318 95 77 57 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 808 1428 Size: 808 620 XbaI T'CTAG_A Cuts at: 0 291 358 1428 Size: 291 67 1070 Fragments arranged by size: 1070 291 67 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1410 1428 Size: 1410 18 Enzymes that do cut: AcuI AflIII AloI AlwNI ApaLI AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmBI Bsp1286I BspMI BsrDI BsrGI BssSI BstAPI BstEII BstYI Bsu36I BtgI BtgZI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII KasI MscI MslI MspA1I NarI PflMI PfoI PmlI PpiI PpuMI PstI PvuII SacI SanDI SexAI SfcI SfoI SmlI StyI TaqII TatI TstI Tth111I XbaI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BlpI BmtI BsaBI BsiEI BsmI BspEI BspHI BsrBI BsrFI BssHII BstBI BstXI BstZ17I BtsI ClaI CspCI DrdI EagI EciI EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI XhoI XmaI XmnI ZraI