(Circular) (Six-base) MAPSORT of: pFN21AE3205.seq Check: 1745 from: 1 to: 6133 pFN21AE3205 6133 bp With 182 enzymes: SgfI * March 28, 2012 14:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3943 278 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3476 1937 Size: 1539 4594 Acc65I G'GTAC_C Cuts at: 3454 3454 Size: 6133 AclI AA'CG_TT Cuts at: 1563 4260 4633 1563 Size: 2697 373 3063 Fragments arranged by size: 3063 2697 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2002 2306 2467 4196 5580 5848 1632 Size: 370 304 161 1729 1384 268 1917 Fragments arranged by size: 1917 1729 1384 370 304 268 161 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5936 AflIII A'CryG_T Cuts at: 1203 2970 5032 6009 1203 Size: 1767 2062 977 1327 Fragments arranged by size: 2062 1767 1327 977 AgeI A'CCGG_T Cuts at: 4964 4964 Size: 6133 AhdI GACnn_n'nnGTC Cuts at: 4862 4862 Size: 6133 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6133 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6133 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2957 2989 2957 Size: 32 6101 AlwNI CAG_nnn'CTG Cuts at: 2232 2335 2408 2551 3085 3130 3325 3876 5448 Size: 103 73 143 534 45 195 551 1572 Cuts at: 5448 2232 Size: 2917 Fragments arranged by size: 2917 1572 551 534 195 143 103 73 45 ApaLI G'TGCA_C Cuts at: 2447 4190 5346 2447 Size: 1743 1156 3234 Fragments arranged by size: 3234 1743 1156 ApoI r'AATT_y Cuts at: 1493 3442 3668 3820 5929 1493 Size: 1949 226 152 2109 1697 Fragments arranged by size: 2109 1949 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4687 160 Size: 872 3655 1606 Fragments arranged by size: 3655 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 AvaI C'yCGr_G Cuts at: 1870 1943 2950 2988 3299 3458 1870 Size: 73 1007 38 311 159 4545 Fragments arranged by size: 4545 1007 311 159 73 38 BamHI G'GATC_C Cuts at: 3463 3830 3463 Size: 367 5766 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2317 2909 3295 3454 4909 Size: 325 217 627 530 592 386 159 1455 Cuts at: 4909 618 Size: 1842 Fragments arranged by size: 1842 1455 627 592 530 386 325 217 159 BanII G_rGCy'C Cuts at: 1391 2666 2928 2951 2997 3252 3452 1391 Size: 1275 262 23 46 255 200 4072 Fragments arranged by size: 4072 1275 262 255 200 46 23 BbeI G_GCGC'C Cuts at: 3299 3299 Size: 6133 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2449 2606 928 Size: 971 550 157 4455 Fragments arranged by size: 4455 971 550 157 BbvCI CC'TCA_GC Cuts at: 3015 3291 3015 Size: 276 5857 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3138 3172 4324 4358 1903 Size: 34 1201 34 1152 34 3678 Fragments arranged by size: 3678 1201 1152 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4025 5235 4025 Size: 1210 4923 BclI T'GATC_A Cuts at: 1548 1548 Size: 6133 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3195 3207 4744 Size: 107 122 71 631 605 1523 12 1537 Cuts at: 4744 136 Size: 1525 Fragments arranged by size: 1537 1525 1523 631 605 122 107 71 12 BglII A'GATC_T Cuts at: 2715 6128 2715 Size: 3413 2720 BlpI GC'TnA_GC Cuts at: 3550 3550 Size: 6133 Bme1580I G_kGCm'C Cuts at: 1790 2451 3350 4194 5350 1790 Size: 661 899 844 1156 2573 Fragments arranged by size: 2573 1156 899 844 661 BmgBI CAC'GTC Cuts at: 1318 2725 1318 Size: 1407 4726 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2316 3864 4822 Size: 416 53 83 399 24 899 1548 958 Cuts at: 4822 442 Size: 1753 Fragments arranged by size: 1753 1548 958 899 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6133 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2878 3269 2878 Size: 391 5742 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2513 2749 2867 2984 3086 3206 3239 Size: 968 306 236 118 117 102 120 33 Cuts at: 3239 4793 1239 Size: 1554 2579 Fragments arranged by size: 2579 1554 968 306 236 120 118 117 102 33 Bpu10I CC'TnA_GC Cuts at: 3015 3291 3015 Size: 276 5857 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2458 4258 5123 5421 2458 Size: 1800 865 298 3170 Fragments arranged by size: 3170 1800 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2206 2243 2356 4796 882 Size: 617 707 37 113 2440 2219 Fragments arranged by size: 2440 2219 707 617 113 37 BsaAI yAC'GTr Cuts at: 493 1204 2971 3172 3262 3924 493 Size: 711 1767 201 90 662 2702 Fragments arranged by size: 2702 1767 711 662 201 90 BsaBI GATnn'nnATC Cuts at: 6127 6127 Size: 6133 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2859 3296 3940 Size: 53 83 186 936 476 850 437 644 Cuts at: 3940 4322 4957 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 850 644 635 476 437 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3426 4566 4964 5238 5385 5796 3426 Size: 1140 398 274 147 411 3763 Fragments arranged by size: 3763 1140 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2957 2987 3257 3287 1178 Size: 30 165 30 1554 30 270 30 4024 Fragments arranged by size: 4024 1554 270 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2294 2936 3242 1186 Size: 1108 642 306 4077 Fragments arranged by size: 4077 1108 642 306 BseYI C'CCAG_C Cuts at: 2265 2688 3239 5336 5832 2265 Size: 423 551 2097 496 2566 Fragments arranged by size: 2566 2097 551 496 423 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2245 2364 2923 3074 3155 3384 1163 Size: 573 509 119 559 151 81 229 3912 Fragments arranged by size: 3912 573 559 509 229 151 119 81 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3583 4344 5372 1540 Size: 275 186 1582 761 1028 2301 Fragments arranged by size: 2301 1582 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2451 2793 2970 2997 3252 4194 4279 5350 2451 Size: 342 177 27 255 942 85 1071 3234 Fragments arranged by size: 3234 1071 942 342 255 177 85 27 BsiWI C'GTAC_G Cuts at: 3105 3105 Size: 6133 BsmI GAATG_Cn' Cuts at: 3644 3737 3644 Size: 93 6040 BsmBI CGTCTCn'nnnn_ Cuts at: 2070 2866 2070 Size: 796 5337 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2451 2666 2793 2928 2951 2970 2997 Size: 399 661 215 127 135 23 19 27 Cuts at: 2997 3252 3350 3452 4194 4279 5350 1391 Size: 255 98 102 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 661 399 255 215 135 127 102 98 85 27 23 19 BspEI T'CCGG_A Cuts at: 5796 5796 Size: 6133 BspHI T'CATG_A Cuts at: 4022 5752 4022 Size: 1730 4403 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2644 3356 3488 844 Size: 1800 712 132 3489 Fragments arranged by size: 3489 1800 712 132 BsrBI CCG'CTC Cuts at: 1394 1823 4020 1394 Size: 429 2197 3507 Fragments arranged by size: 3507 2197 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3105 4628 4802 65 Size: 1183 1857 1523 174 1396 Fragments arranged by size: 1857 1523 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4777 4964 1700 Size: 110 2967 187 2869 Fragments arranged by size: 2967 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3327 96 Size: 3231 2902 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6133 BssSI C'ACGA_G Cuts at: 2276 4193 5205 2276 Size: 1917 1012 3204 Fragments arranged by size: 3204 1917 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2232 794 Size: 1438 4695 BstBI TT'CG_AA Cuts at: 4937 4937 Size: 6133 BstEII G'GTnAC_C Cuts at: 1184 3019 1184 Size: 1835 4298 BstYI r'GATC_y Cuts at: 1972 2583 2715 3463 3830 4215 4232 5684 5770 Size: 611 132 748 367 385 17 1452 86 Cuts at: 5770 5782 6128 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 748 611 385 367 346 132 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3035 3193 3035 Size: 158 5975 BtgI C'CryG_G Cuts at: 513 1065 2004 3396 513 Size: 552 939 1392 3250 Fragments arranged by size: 3250 1392 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2355 487 Size: 683 56 1129 4265 Fragments arranged by size: 4265 1129 683 56 BtsI GCAGTG_nn' Cuts at: 3649 4443 4463 3649 Size: 794 20 5319 Fragments arranged by size: 5319 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6133 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6098 DraI TTT'AAA Cuts at: 2434 3437 2434 Size: 1003 5130 DraIII CAC_nnn'GTG Cuts at: 3172 3397 3172 Size: 225 5908 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5140 809 Size: 507 3824 1802 Fragments arranged by size: 3824 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2118 3580 4469 Size: 54 1425 50 275 18 288 1462 889 Cuts at: 4469 6098 8 Size: 1629 43 Fragments arranged by size: 1629 1462 1425 889 288 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3580 1537 Size: 275 1768 4090 Fragments arranged by size: 4090 1768 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2847 4063 1353 Size: 1494 1216 3423 Fragments arranged by size: 3423 1494 1216 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4720 5094 5240 387 Size: 1064 138 3131 374 146 1280 Fragments arranged by size: 3131 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2995 3250 2995 Size: 255 5878 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2002 2207 2306 2467 2513 2749 2867 Size: 393 370 205 99 161 46 236 118 Cuts at: 2867 2984 3086 3206 3239 4196 4793 5580 5848 Size: 117 102 120 33 957 597 787 268 Cuts at: 5848 1239 Size: 1524 Fragments arranged by size: 1524 957 787 597 393 370 268 236 205 161 120 118 117 102 99 46 33 EcoNI CCTnn'n_nnAGG Cuts at: 2473 2672 2473 Size: 199 5934 EcoO109I rG'GnC_Cy Cuts at: 2175 3226 2175 Size: 1051 5082 EcoRI G'AATT_C Cuts at: 3442 3820 3442 Size: 378 5755 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2698 2698 Size: 6133 FspI TGC'GCA Cuts at: 4639 4639 Size: 6133 HaeII r_GCGC'y Cuts at: 1678 2030 2552 3157 3299 5280 1678 Size: 352 522 605 142 1981 2531 Fragments arranged by size: 2531 1981 605 522 352 142 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4774 4806 4848 4880 1373 Size: 32 135 32 3202 32 42 32 2626 Fragments arranged by size: 3202 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3477 3723 669 Size: 988 281 1539 246 3079 Fragments arranged by size: 3079 1539 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6133 HpaI GTT'AAC Cuts at: 3723 3723 Size: 6133 KasI G'GCGC_C Cuts at: 3295 3295 Size: 6133 KpnI G_GTAC'C Cuts at: 3458 3458 Size: 6133 MfeI C'AATT_G Cuts at: 3732 3732 Size: 6133 MluI A'CGCG_T Cuts at: 6009 6009 Size: 6133 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5247 5431 1305 Size: 3942 184 2007 Fragments arranged by size: 3942 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2120 10 Size: 54 1425 343 288 4023 Fragments arranged by size: 4023 1425 343 288 54 MslI CAynn'nnrTG Cuts at: 518 1166 2732 4091 4450 4609 518 Size: 648 1566 1359 359 159 2042 Fragments arranged by size: 2042 1566 1359 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2022 2035 2229 2392 2907 3549 4226 5374 Size: 416 13 194 163 515 642 677 1148 Cuts at: 5374 5619 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 642 515 416 245 194 163 13 NarI GG'CG_CC Cuts at: 3296 3296 Size: 6133 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4642 Fragments arranged by size: 4642 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6133 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6133 NotI GC'GGCC_GC Cuts at: 3580 3580 Size: 6133 NspI r_CATG'y Cuts at: 1132 1229 3491 5036 1132 Size: 97 2262 1545 2229 Fragments arranged by size: 2262 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6133 PciI A'CATG_T Cuts at: 5032 5032 Size: 6133 PflMI CCAn_nnn'nTGG Cuts at: 1108 3130 3172 3262 3397 1108 Size: 2022 42 90 135 3844 Fragments arranged by size: 3844 2022 135 90 42 PfoI T'CCnGG_A Cuts at: 2490 3051 2490 Size: 561 5572 PmeI GTTT'AAAC Cuts at: 3437 3437 Size: 6133 PmlI CAC'GTG Cuts at: 2971 3262 2971 Size: 291 5842 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2957 2989 4202 4234 5715 5747 2957 Size: 32 1213 32 1481 32 3343 Fragments arranged by size: 3343 1481 1213 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2175 2175 Size: 6133 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6133 PsiI TTA'TAA Cuts at: 3703 3703 Size: 6133 PstI C_TGCA'G Cuts at: 830 2034 2543 2920 3345 3485 830 Size: 1204 509 377 425 140 3478 Fragments arranged by size: 3478 1204 509 425 377 140 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6133 PvuII CAG'CTG Cuts at: 2035 2392 2035 Size: 357 5776 SacI G_AGCT'C Cuts at: 2997 3252 2997 Size: 255 5878 SalI G'TCGA_C Cuts at: 1936 3475 1936 Size: 1539 4594 SanDI GG'GwC_CC Cuts at: 2175 2175 Size: 6133 SbfI CC_TGCA'GG Cuts at: 3485 3485 Size: 6133 ScaI AGT'ACT Cuts at: 4381 6093 4381 Size: 1712 4421 SexAI A'CCwGG_T Cuts at: 3129 3129 Size: 6133 SfcI C'TryA_G Cuts at: 826 1045 2030 2400 2539 2916 3341 3481 4616 Size: 219 985 370 139 377 425 140 1135 Cuts at: 4616 5297 5488 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 985 681 425 377 370 219 191 140 139 SfoI GGC'GCC Cuts at: 3297 3297 Size: 6133 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6133 SmaI CCC'GGG Cuts at: 1872 3460 1872 Size: 1588 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2473 4237 5138 5400 820 Size: 197 926 530 1764 901 262 1553 Fragments arranged by size: 1764 1553 926 901 530 262 197 SnaBI TAC'GTA Cuts at: 493 3924 493 Size: 3431 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6133 SphI G_CATG'C Cuts at: 1132 3491 1132 Size: 2359 3774 SspI AAT'ATT Cuts at: 5 52 4057 5 Size: 47 4005 2081 Fragments arranged by size: 4005 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6133 StyI C'CwwG_G Cuts at: 513 1065 2004 2196 3572 513 Size: 552 939 192 1376 3074 Fragments arranged by size: 3074 1376 939 552 192 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2279 2335 2338 4160 4177 4330 4515 Size: 1255 699 56 3 1822 17 153 185 Cuts at: 4515 4960 325 Size: 445 1498 Fragments arranged by size: 1822 1498 1255 699 445 185 153 56 17 3 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3327 4379 6091 Size: 274 80 33 51 1443 1350 1052 1712 Cuts at: 6091 96 Size: 138 Fragments arranged by size: 1712 1443 1350 1052 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2100 2132 2885 2917 2994 3026 3344 3376 6093 Size: 32 753 32 77 32 318 32 2717 Cuts at: 6093 6125 2100 Size: 32 2108 Fragments arranged by size: 2717 2108 753 318 77 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2813 1316 Size: 1497 4636 XbaI T'CTAG_A Cuts at: 2296 2363 3469 2296 Size: 67 1106 4960 Fragments arranged by size: 4960 1106 67 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3415 3415 Size: 6133 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6133 XmaI C'CCGG_G Cuts at: 1870 3458 1870 Size: 1588 4545 XmnI GAAnn'nnTTC Cuts at: 4262 4262 Size: 6133 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3941 276 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BfrBI BstXI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SapI SfiI SrfI SwaI