Restriction map for
pFN21AE3205
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaBI BseAI BsiEI
BsmI BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BssHII Bst1107I BstBI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflIII 965 ACGTGT A^C[GA][CT]GT
Alw44I 442 GTGCAC G^TGCAC
AlwI 578 GGATC GGATC(4/5)
ApaLI 442 GTGCAC G^TGCAC
AspI 805 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 113 TGGCCA TGG^CCA
BbeI 1290 GGCGCC GGCGC^C
BcgI 1144 CGAGGGCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 710 AGATCT A^GATCT
BsaWI 1421 TCCGGT [AT]^CCGG[AT]
BsiWI 1100 CGTACG C^GTACG
Bsp1407I 1322 TGTACA T^GTACA
BsrDI 1093 GCAATG GCAATG(2/0)
BsrGI 1322 TGTACA T^GTACA
BssSI 271 CACGAG CACGAG(-5/-1)
BstAPI 221 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1014 GGTTACC G^GT[ACGT]ACC
BstPI 1014 GGTTACC G^GT[ACGT]ACC
DraI 427 TTTAAA TTT^AAA
DsaI 1391 CCGTGG C^C[GA][CT]GG
EaeI 113 TGGCCA [CT]^GGCC[GA]
EarI 847 GAAGAG CTCTTC(1/4)
EcoO65I 1014 GGTTACC G^GT[ACGT]ACC
EcoT14I 191 CCAAGG C^C[AT][AT]GG
KasI 1290 GGCGCC G^GCGCC
Ksp632I 847 GAAGAG CTCTTC(1/4)
MluNI 113 TGGCCA TGG^CCA
MscI 113 TGGCCA TGG^CCA
MslI 723 CACAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1290 GGCGCC GG^CGCC
PflFI 805 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 169 GGGACCC [GA]G^G[AT]CC[CT]
SexAI 1124 ACCTGGT A^CC[AT]GGT
SfoI 1290 GGCGCC GGC^GCC
SmlI 468 CTCAAG C^T[CT][GA]AG
SspBI 1322 TGTACA T^GTACA
StyI 191 CCAAGG C^C[AT][AT]GG
TaqI 455 TCGA T^CGA
Tth111I 805 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 455 TCGA T^CGA
XcmI 1403 CCAGCACTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 35 enzymes) #
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AccII 978 CGCG CG^CG
1302 CGCG
AocI 1029 CCTGAGG CC^T[ACGT]AGG
1187 CCTCAGG
BbrPI 964 CACGTG CAC^GTG
1255 CACGTG
BbsI 594 GAAGAC GAAGAC(2/6)
451 GTCTTC
BbvCI 1009 GCTGAGG CCTCAGC(-5/-2)
1285 GCTGAGG
BfaI 292 CTAG C^TAG
359 CTAG
BglI 1184 GCCCCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1196 GCCTGAACGGC
BpuAI 594 GAAGAC GAAGAC(2/6)
451 GTCTTC
BsmBI 59 CGTCTC CGTCTC(1/5)
855 CGTCTC
BsmFI 169 GGGAC GGGAC(10/14)
639 GTCCC
BspMI 648 GCAGGT ACCTGC(4/8)
1360 GCAGGT
BstUI 978 CGCG CG^CG
1302 CGCG
BstYI 578 GGATCT [GA]^GATC[CT]
710 AGATCT
Bsu36I 1029 CCTGAGG CC^T[ACGT]AGG
1187 CCTCAGG
DpnII 579 GATC ^GATC
711 GATC
DraII 169 GGGACCC [GA]G^G[ACGT]CC[CT]
1220 AGGCCCC
DraIII 1162 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
1387 CACGCCGTG
Eco81I 1029 CCTGAGG CC^T[ACGT]AGG
1187 CCTCAGG
EcoNI 464 CCTTCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
663 CCTGGTCAAGG
EcoO109I 169 GGGACCC [GA]G^G[ACGT]CC[CT]
1220 AGGCCCC
MaeI 292 CTAG C^TAG
359 CTAG
MboI 579 GATC ^GATC
711 GATC
MflI 578 GGATCT [GA]^GATC[CT]
710 AGATCT
MvnI 978 CGCG CG^CG
1302 CGCG
NdeII 579 GATC ^GATC
711 GATC
PleI 597 GACTC GAGTC(4/5)
1348 GACTC
PmaCI 964 CACGTG CAC^GTG
1255 CACGTG
PmlI 964 CACGTG CAC^GTG
1255 CACGTG
PvuII 28 CAGCTG CAG^CTG
385 CAGCTG
SacI 988 GAGCTC GAGCT^C
1243 GAGCTC
Sau3AI 579 GATC ^GATC
711 GATC
Tsp45I 721 GTCAC ^GT[GC]AC
1054 GTGAC
Tsp509I 153 AATT ^AATT
413 AATT
XbaI 291 TCTAGA T^CTAGA
358 TCTAGA
XhoII 578 GGATCT [GA]^GATC[CT]
710 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboII MnlI MseI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV PflMI PstI RsaI Sau96I
ScrFI SfaNI SfcI TfiI Tru9I
TseI TspRI Van91I