RNF128 ORF clone pFN21AE3187

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE3187

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Clone info.

Species

human

Clone Name

pFN21AE3187 Sequence (with vector) Restriction Map

Product ID

FHC27454

ORF Sequence

DNA sequence (1284 bp)
Protein sequence (428 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3187

Symbol

RNF128

Alias : GRAIL

Description

ring finger protein 128, E3 ubiquitin protein ligase, transcript variant 1

Original Clone ID

cp93187

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1284 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 428 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS14521.1 5.3e-163 100.0 RNF128 CCDS14520.1 7.8e-105 80.5 RNF128 CCDS5784.1 1.5e-51 50.1 RNF133 CCDS2051.1 9.7e-45 41.3 RNF149 CCDS34065.1 2.7e-44 41.6 RNF150 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003137 118 182 PF02225 Protease-associated domain IPR018957 277 317 PF00097 Zinc finger HMMSmart IPR001841 277 317 SM00184 Zinc finger ProfileScan IPR001841 277 318 PS50089 Zinc finger
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 14 GCGFSRLLAWCFLLALSPQAPGS 36 SECONDARY 23 2 207 SIFFVSVSFFIITAATVGYFIFY 229 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_919445 428 300439 100.0 100.0 NP_078815 402 300439 80.5 90.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3187 Download

>KIEE3187  1284 bp
ATGGGGCCGCCGCCTGGGGCCGGGGTCTCCTGCCGCGGTGGCTGCGGCTTTTCCAGATTG
CTGGCATGGTGCTTCCTGCTGGCCCTGAGTCCGCAGGCACCCGGTTCCCGGGGGGCTGAA
GCAGTGTGGACCGCGTACCTCAACGTGTCCTGGCGGGTTCCGCACACGGGAGTGAACCGT
ACGGTGTGGGAGCTGAGCGAGGAGGGCGTGTACGGCCAGGACTCGCCGCTGGAGCCTGTG
GCTGGGGTCCTGGTACCGCCCGACGGGCCCGGGGCGCTTAACGCCTGTAACCCGCACACG
AATTTCACGGTGCCCACGGTTTGGGGAAGCACCGTGCAAGTCTCTTGGTTGGCCCTCATC
CAACGCGGCGGGGGCTGCACCTTCGCAGACAAGATCCATCTGGCTTATGAGAGAGGGGCG
TCTGGAGCCGTCATCTTTAACTTCCCCGGGACCCGCAATGAGGTCATCCCCATGTCTCAC
CCGGGTGCAGTAGACATTGTTGCAATCATGATCGGCAATCTGAAAGGCACAAAAATTCTG
CAATCTATTCAAAGAGGCATACAAGTGACAATGGTCATAGAAGTAGGGAAAAAACATGGC
CCTTGGGTGAATCACTATTCAATTTTTTTCGTTTCTGTGTCCTTTTTTATTATTACGGCG
GCAACTGTGGGCTATTTTATCTTTTATTCTGCTCGAAGGCTACGGAATGCAAGAGCTCAA
AGCAGGAAGCAGAGGCAATTAAAGGCAGATGCTAAAAAAGCTATTGGAAGGCTTCAACTA
CGCACACTGAAACAAGGAGACAAGGAAATTGGCCCTGATGGAGATAGTTGTGCTGTGTGC
ATTGAATTGTATAAACCAAATGATTTGGTACGCATCTTAACGTGCAACCATATTTTCCAT
AAGACATGTGTTGACCCATGGCTGTTAGAACACAGGACTTGCCCCATGTGCAAATGTGAC
ATACTCAAAGCTTTGGGAATTGAGGTGGATGTTGAAGATGGATCAGTGTCTTTACAAGTC
CCTGTATCCAATGAAATATCTAATAGTGCCTCCTCCCATGAAGAGGATAATCGCAGCGAG
ACCGCATCATCTGGATATGCTTCAGTACAGGGAACAGATGAACCGCCTCTGGAGGAACAC
GTGCAGTCAACAAATGAAAGTCTACAGCTGGTAAACCATGAAGCAAATTCTGTGGCAGTG
GATGTTATTCCTCATGTTGACAACCCAACCTTTGAAGAAGACGAAACTCCTAATCAAGAG
ACTGCTGTTCGAGAAATTAAATCT

ORF Protein

Cloned ORF protein sequence for pFN21AE3187 Download

>KIEE3187  428 aa
MGPPPGAGVSCRGGCGFSRLLAWCFLLALSPQAPGSRGAEAVWTAYLNVSWRVPHTGVNR
TVWELSEEGVYGQDSPLEPVAGVLVPPDGPGALNACNPHTNFTVPTVWGSTVQVSWLALI
QRGGGCTFADKIHLAYERGASGAVIFNFPGTRNEVIPMSHPGAVDIVAIMIGNLKGTKIL
QSIQRGIQVTMVIEVGKKHGPWVNHYSIFFVSVSFFIITAATVGYFIFYSARRLRNARAQ
SRKQRQLKADAKKAIGRLQLRTLKQGDKEIGPDGDSCAVCIELYKPNDLVRILTCNHIFH
KTCVDPWLLEHRTCPMCKCDILKALGIEVDVEDGSVSLQVPVSNEISNSASSHEEDNRSE
TASSGYASVQGTDEPPLEEHVQSTNESLQLVNHEANSVAVDVIPHVDNPTFEEDETPNQE
TAVREIKS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3187 Download

>pFN21AE3187  5989 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGGCCGCCGCCTGGGGCCGGGGTCTCCTGCCG
CGGTGGCTGCGGCTTTTCCAGATTGCTGGCATGGTGCTTCCTGCTGGCCCTGAGTCCGCA
GGCACCCGGTTCCCGGGGGGCTGAAGCAGTGTGGACCGCGTACCTCAACGTGTCCTGGCG
GGTTCCGCACACGGGAGTGAACCGTACGGTGTGGGAGCTGAGCGAGGAGGGCGTGTACGG
CCAGGACTCGCCGCTGGAGCCTGTGGCTGGGGTCCTGGTACCGCCCGACGGGCCCGGGGC
GCTTAACGCCTGTAACCCGCACACGAATTTCACGGTGCCCACGGTTTGGGGAAGCACCGT
GCAAGTCTCTTGGTTGGCCCTCATCCAACGCGGCGGGGGCTGCACCTTCGCAGACAAGAT
CCATCTGGCTTATGAGAGAGGGGCGTCTGGAGCCGTCATCTTTAACTTCCCCGGGACCCG
CAATGAGGTCATCCCCATGTCTCACCCGGGTGCAGTAGACATTGTTGCAATCATGATCGG
CAATCTGAAAGGCACAAAAATTCTGCAATCTATTCAAAGAGGCATACAAGTGACAATGGT
CATAGAAGTAGGGAAAAAACATGGCCCTTGGGTGAATCACTATTCAATTTTTTTCGTTTC
TGTGTCCTTTTTTATTATTACGGCGGCAACTGTGGGCTATTTTATCTTTTATTCTGCTCG
AAGGCTACGGAATGCAAGAGCTCAAAGCAGGAAGCAGAGGCAATTAAAGGCAGATGCTAA
AAAAGCTATTGGAAGGCTTCAACTACGCACACTGAAACAAGGAGACAAGGAAATTGGCCC
TGATGGAGATAGTTGTGCTGTGTGCATTGAATTGTATAAACCAAATGATTTGGTACGCAT
CTTAACGTGCAACCATATTTTCCATAAGACATGTGTTGACCCATGGCTGTTAGAACACAG
GACTTGCCCCATGTGCAAATGTGACATACTCAAAGCTTTGGGAATTGAGGTGGATGTTGA
AGATGGATCAGTGTCTTTACAAGTCCCTGTATCCAATGAAATATCTAATAGTGCCTCCTC
CCATGAAGAGGATAATCGCAGCGAGACCGCATCATCTGGATATGCTTCAGTACAGGGAAC
AGATGAACCGCCTCTGGAGGAACACGTGCAGTCAACAAATGAAAGTCTACAGCTGGTAAA
CCATGAAGCAAATTCTGTGGCAGTGGATGTTATTCCTCATGTTGACAACCCAACCTTTGA
AGAAGACGAAACTCCTAATCAAGAGACTGCTGTTCGAGAAATTAAATCTGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT