Restriction map for
pFN21AE3187
(Circular) (Six-base) MAPSORT of: pFN21AE3187.seq Check: 9390 from: 1 to: 5989
pFN21AE3187 5989 bp
With 182 enzymes: SgfI *
March 28, 2012 12:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3799 278
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2496 3166 3332 1937
Size: 559 670 166 4594
Fragments arranged by size:
4594 670 559 166
Acc65I G'GTAC_C
Cuts at: 2257 3310 2257
Size: 1053 4936
AclI AA'CG_TT
Cuts at: 1563 4116 4489 1563
Size: 2553 373 3063
Fragments arranged by size:
3063 2553 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2142 3090 4052 5436 5704 1632
Size: 510 948 962 1384 268 1917
Fragments arranged by size:
1917 1384 962 948 510 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5792
AflIII A'CryG_T
Cuts at: 1203 2148 2909 3142 4888 5865 1203
Size: 945 761 233 1746 977 1327
Fragments arranged by size:
1746 1327 977 945 761 233
AgeI A'CCGG_T
Cuts at: 4820 4820
Size: 5989
AhdI GACnn_n'nnGTC
Cuts at: 4718 4718
Size: 5989
AleI CACnn'nnGTG
Cuts at: 1166 2174 2487 1166
Size: 1008 313 4668
Fragments arranged by size:
4668 1008 313
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5989
AlwNI CAG_nnn'CTG
Cuts at: 3732 5304 3732
Size: 1572 4417
ApaI G_GGCC'C
Cuts at: 2274 2274
Size: 5989
ApaLI G'TGCA_C
Cuts at: 4046 5202 4046
Size: 1156 4833
ApoI r'AATT_y
Cuts at: 1493 2305 2538 3190 3298 3524 3676 5785 1493
Size: 812 233 652 108 226 152 2109 1697
Fragments arranged by size:
2109 1697 812 652 233 226 152 108
AseI AT'TA_AT
Cuts at: 160 1032 4543 160
Size: 872 3511 1606
Fragments arranged by size:
3511 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
AvaI C'yCGr_G
Cuts at: 1870 1943 2112 2273 2450 2485 3314 1870
Size: 73 169 161 177 35 829 4545
Fragments arranged by size:
4545 829 177 169 161 73 35
BamHI G'GATC_C
Cuts at: 3319 3686 3319
Size: 367 5622
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2101 2257 2314 3310 4765
Size: 325 217 627 314 156 57 996 1455
Cuts at: 4765 618
Size: 1842
Fragments arranged by size:
1842 1455 996 627 325 314 217 156
57
BanII G_rGCy'C
Cuts at: 1391 2274 2722 3308 1391
Size: 883 448 586 4072
Fragments arranged by size:
4072 883 586 448
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3249 928
Size: 971 1350 3668
Fragments arranged by size:
3668 1350 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4180 4214 1903
Size: 34 2243 34 3678
Fragments arranged by size:
3678 2243 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3040 3881 5091 3040
Size: 841 1210 3938
Fragments arranged by size:
3938 1210 841
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5989
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2043 2269 4600
Size: 107 122 71 631 605 371 226 2331
Cuts at: 4600 136
Size: 1525
Fragments arranged by size:
2331 1525 631 605 371 226 122 107
71
BglII A'GATC_T
Cuts at: 5984 5984
Size: 5989
BlpI GC'TnA_GC
Cuts at: 2198 3406 2198
Size: 1208 4781
Bme1580I G_kGCm'C
Cuts at: 1790 2274 2319 4050 5206 1790
Size: 484 45 1731 1156 2573
Fragments arranged by size:
2573 1731 1156 484 45
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5989
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3720 4678 442
Size: 416 53 83 399 24 2303 958 1753
Fragments arranged by size:
2303 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5989
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2255 2448 3155 4649 1239
Size: 1016 193 707 1494 2579
Fragments arranged by size:
2579 1494 1016 707 193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4114 4979 5277 4114
Size: 865 298 4826
Fragments arranged by size:
4826 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2035 3077 4652 882
Size: 617 536 1042 1575 2219
Fragments arranged by size:
2219 1575 1042 617 536
BsaAI yAC'GTr
Cuts at: 493 1204 3145 3780 493
Size: 711 1941 635 2702
Fragments arranged by size:
2702 1941 711 635
BsaBI GATnn'nnATC
Cuts at: 5983 5983
Size: 5989
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2423 3796 4178 4813
Size: 53 83 186 936 890 1373 382 635
Cuts at: 4813 275
Size: 1451
Fragments arranged by size:
1451 1373 936 890 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4422 4820 5094 5241 5652 4422
Size: 398 274 147 411 4759
Fragments arranged by size:
4759 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2198 2228 1178
Size: 30 165 30 795 30 4939
Fragments arranged by size:
4939 795 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2219 3046 1186
Size: 1033 827 4129
Fragments arranged by size:
4129 1033 827
BseYI C'CCAG_C
Cuts at: 2246 5192 5688 2246
Size: 2946 496 2547
Fragments arranged by size:
2946 2547 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2365 2511 3167 1163
Size: 573 629 146 656 3985
Fragments arranged by size:
3985 656 629 573 146
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3439 4200 5228 1540
Size: 275 186 1438 761 1028 2301
Fragments arranged by size:
2301 1438 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2722 4050 4135 5206 2722
Size: 1328 85 1071 3505
Fragments arranged by size:
3505 1328 1071 85
BsiWI C'GTAC_G
Cuts at: 2183 2183
Size: 5989
BsmI GAATG_Cn'
Cuts at: 2716 3500 3593 2716
Size: 784 93 5112
Fragments arranged by size:
5112 784 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2274 2319 2722 3308 4050 4135 5206
Size: 399 484 45 403 586 742 85 1071
Cuts at: 5206 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 586 484 403 399 85
45
BspEI T'CCGG_A
Cuts at: 5652 5652
Size: 5989
BspHI T'CATG_A
Cuts at: 2511 3878 5608 2511
Size: 1367 1730 2892
Fragments arranged by size:
2892 1730 1367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3344 844
Size: 2500 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3876 1394
Size: 429 2053 3507
Fragments arranged by size:
3507 2053 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2467 4484 4658 65
Size: 1183 1219 2017 174 1396
Fragments arranged by size:
2017 1396 1219 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4633 4820 1700
Size: 110 2823 187 2869
Fragments arranged by size:
2869 2823 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5989
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5989
BssSI C'ACGA_G
Cuts at: 4049 5061 4049
Size: 1012 4977
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5989
BstBI TT'CG_AA
Cuts at: 4793 4793
Size: 5989
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5989
BstXI CCAn_nnnn'nTGG
Cuts at: 2065 2065
Size: 5989
BstYI r'GATC_y
Cuts at: 1972 2397 3319 3686 4071 4088 5540 5626 5638
Size: 425 922 367 385 17 1452 86 12
Cuts at: 5638 5984 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 922 425 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2038 2319 2921 513
Size: 552 939 34 281 602 3581
Fragments arranged by size:
3581 939 602 552 281 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5250
Fragments arranged by size:
5250 683 56
BtsI GCAGTG_nn'
Cuts at: 2133 3207 3505 4299 4319 2133
Size: 1074 298 794 20 3803
Fragments arranged by size:
3803 1074 794 298 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5989
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3177 3212 529
Size: 35 2613 35 3306
Fragments arranged by size:
3306 2613 35 35
DraI TTT'AAA
Cuts at: 3293 3293
Size: 5989
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4996 809
Size: 507 3680 1802
Fragments arranged by size:
3680 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2218 3436 4325
Size: 54 1425 50 275 18 388 1218 889
Cuts at: 4325 5954 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1218 889 388 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3436 1537
Size: 275 1624 4090
Fragments arranged by size:
4090 1624 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3060 3919 1353
Size: 1707 859 3423
Fragments arranged by size:
3423 1707 859
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4576 4950 5096 387
Size: 1064 138 2987 374 146 1280
Fragments arranged by size:
2987 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2720 2720
Size: 5989
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2142 2255 2448 3090 3155 4052 4649
Size: 393 510 113 193 642 65 897 597
Cuts at: 4649 5436 5704 1239
Size: 787 268 1524
Fragments arranged by size:
1524 897 787 642 597 510 393 268
193 113 65
EcoO109I rG'GnC_Cy
Cuts at: 2251 2454 2251
Size: 203 5786
EcoRI G'AATT_C
Cuts at: 3298 3676 3298
Size: 378 5611
FspI TGC'GCA
Cuts at: 4495 4495
Size: 5989
HaeII r_GCGC'y
Cuts at: 1678 2282 5136 1678
Size: 604 2854 2531
Fragments arranged by size:
2854 2531 604
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2421 2453 2506 2538 3074
Size: 32 135 32 849 32 53 32 536
Cuts at: 3074 3106 4630 4662 4704 4736 1373
Size: 32 1524 32 42 32 2626
Fragments arranged by size:
2626 1524 849 536 135 53 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2917 3153 3223 3333 3579 669
Size: 988 281 979 236 70 110 246 3079
Fragments arranged by size:
3079 988 979 281 246 236 110 70
HindIII A'AGCT_T
Cuts at: 748 2973 748
Size: 2225 3764
HpaI GTT'AAC
Cuts at: 3579 3579
Size: 5989
KpnI G_GTAC'C
Cuts at: 2261 3314 2261
Size: 1053 4936
MfeI C'AATT_G
Cuts at: 3588 3588
Size: 5989
MluI A'CGCG_T
Cuts at: 5865 5865
Size: 5989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2389 5103 5287 1305
Size: 1084 2714 184 2007
Fragments arranged by size:
2714 2007 1084 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4167
Fragments arranged by size:
4167 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2174 2474 2487 2567 3947 4306 4465
Size: 648 1008 300 13 80 1380 359 159
Cuts at: 4465 518
Size: 2042
Fragments arranged by size:
2042 1380 1008 648 359 300 159 80
13
MspA1I CmG'CkG
Cuts at: 1606 2040 2233 3172 3405 4082 5230 5475 1606
Size: 434 193 939 233 677 1148 245 2120
Fragments arranged by size:
2120 1148 939 677 434 245 233 193
NcoI C'CATG_G
Cuts at: 513 1065 2004 2921 513
Size: 552 939 917 3581
Fragments arranged by size:
3581 939 917 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5989
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5989
NotI GC'GGCC_GC
Cuts at: 3436 3436
Size: 5989
NspI r_CATG'y
Cuts at: 1132 1229 2913 3347 4892 1132
Size: 97 1684 434 1545 2229
Fragments arranged by size:
2229 1684 1545 434 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5989
PciI A'CATG_T
Cuts at: 2909 4888 2909
Size: 1979 4010
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2325 1108
Size: 1217 4772
PmeI GTTT'AAAC
Cuts at: 3293 3293
Size: 5989
PmlI CAC'GTG
Cuts at: 3145 3145
Size: 5989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4058 4090 5571 5603 4058
Size: 32 1481 32 4444
Fragments arranged by size:
4444 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2251 2454 2251
Size: 203 5786
PshAI GACnn'nnGTC
Cuts at: 1613 2576 1613
Size: 963 5026
PsiI TTA'TAA
Cuts at: 3559 3559
Size: 5989
PspOMI G'GGCC_C
Cuts at: 2270 2270
Size: 5989
PstI C_TGCA'G
Cuts at: 830 3341 830
Size: 2511 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5989
PvuII CAG'CTG
Cuts at: 3172 3172
Size: 5989
SacI G_AGCT'C
Cuts at: 2722 2722
Size: 5989
SacII CC_GC'GG
Cuts at: 2041 2041
Size: 5989
SalI G'TCGA_C
Cuts at: 1936 3331 1936
Size: 1395 4594
SanDI GG'GwC_CC
Cuts at: 2454 2454
Size: 5989
SbfI CC_TGCA'GG
Cuts at: 3341 3341
Size: 5989
ScaI AGT'ACT
Cuts at: 4237 5949 4237
Size: 1712 4277
SfcI C'TryA_G
Cuts at: 826 1045 3167 3337 4472 5153 5344 826
Size: 219 2122 170 1135 681 191 1471
Fragments arranged by size:
2122 1471 1135 681 219 191 170
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5989
SmaI CCC'GGG
Cuts at: 1872 2114 2275 2452 2487 3316 1872
Size: 242 161 177 35 829 4545
Fragments arranged by size:
4545 829 242 177 161 35
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4093 4994 5256 820
Size: 197 926 2150 901 262 1553
Fragments arranged by size:
2150 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3780 493
Size: 3287 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5989
SphI G_CATG'C
Cuts at: 1132 3347 1132
Size: 2215 3774
SspI AAT'ATT
Cuts at: 5 52 3913 5
Size: 47 3861 2081
Fragments arranged by size:
3861 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5989
StyI C'CwwG_G
Cuts at: 513 1065 2004 2606 2921 3428 513
Size: 552 939 602 315 507 3074
Fragments arranged by size:
3074 939 602 552 507 315
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2599 4016 4033 4186 4371 4816 325
Size: 1255 1019 1417 17 153 185 445 1498
Fragments arranged by size:
1498 1417 1255 1019 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3109 4235 5947
Size: 274 80 33 51 1443 1132 1126 1712
Cuts at: 5947 96
Size: 138
Fragments arranged by size:
1712 1443 1132 1126 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2322 2354 2973 3005 5949 5981 2322
Size: 32 619 32 2944 32 2330
Fragments arranged by size:
2944 2330 619 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5989
XbaI T'CTAG_A
Cuts at: 3325 3325
Size: 5989
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5989
XmaI C'CCGG_G
Cuts at: 1870 2112 2273 2450 2485 3314 1870
Size: 242 161 177 35 829 4545
Fragments arranged by size:
4545 829 242 177 161 35
XmnI GAAnn'nnTTC
Cuts at: 4118 4118
Size: 5989
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3797 276
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PspXI PsrI RsrII SapI
SexAI SfiI SfoI SrfI SwaI XcmI