################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BamHI BbeI BbvCI BcgI BclI BfaI BfrI BglII BlnI BsaBI BsaWI BseAI BsiEI BsmBI Bsp1407I BspDI BspEI BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HpaI KasI MaeI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PstI PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 252 GGTACC G^GTACC AcyI 417 GGCGTC G[GA]^CG[CT]C ApaI 265 GGGCCC GGGCC^C Asp718I 252 GGTACC G^GTACC AspHI 713 GAGCTC G[AT]GC[AT]^C BbrPI 1138 CACGTG CAC^GTG BbsI 1237 GAAGAC GAAGAC(2/6) BciVI 1024 GTATCC GTATCC(6/5) BlpI 192 GCTGAGC GC^T[ACGT]AGC Bpu1102I 192 GCTGAGC GC^T[ACGT]AGC BpuAI 1237 GAAGAC GAAGAC(2/6) BsaAI 1138 CACGTG [CT]AC^GT[GA] BsaHI 417 GGCGTC G[GA]^CG[CT]C BsiHKAI 713 GAGCTC G[AT]GC[AT]^C BsiWI 178 CGTACG C^GTACG BsmI 705 GAATGC GAATGC(1/-1) BspHI 506 TCATGA T^CATGA BspLU11I 904 ACATGT A^CATGT BsrDI 455 GCAATG GCAATG(2/0) BstXI 53 CCAGATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 392 AGATCC [GA]^GATC[CT] CelII 192 GCTGAGC GC^T[ACGT]AGC CfoI 274 GCGC GCG^C EaeI 213 CGGCCA [CT]^GGCC[GA] EarI 1060 GAAGAG CTCTTC(1/4) HaeII 273 GGCGCT [GA]GCGC^[CT] HgaI 418 GCGTC GACGC(5/10) HhaI 274 GCGC GCG^C Hin1I 417 GGCGTC G[GA]^CG[CT]C HinP1I 274 GCGC G^CGC HindIII 968 AAGCTT A^AGCTT KpnI 252 GGTACC GGTAC^C Ksp632I 1060 GAAGAG CTCTTC(1/4) KspI 33 CCGCGG CCGC^GG MflI 392 AGATCC [GA]^GATC[CT] NcoI 916 CCATGG C^CATGG NspI 904 ACATGT [GA]CATG^[CT] PflMI 314 CCACGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1138 CACGTG CAC^GTG PmlI 1138 CACGTG CAC^GTG PshAI 567 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1165 CAGCTG CAG^CTG RcaI 506 TCATGA T^CATGA SacI 713 GAGCTC GAGCT^C SacII 33 CCGCGG CCGC^GG SfcI 1162 CTACAG C^T[GA][CT]AG TfiI 609 GAATC G^A[AT]TC Van91I 314 CCACGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 392 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccI 490 GTAGAC GT^[AC][GT]AC 1160 GTCTAC AlwI 393 GATCC GGATC(4/5) 1000 GGATC BanII 265 GGGCCC G[GA]GC[CT]^C 713 GAGCTC BglI 32 GCCGCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 258 GCCCGACGGGC BsaI 1078 GAGACC GGTCTC(1/5) 24 GGTCTC BseRI 1050 CTCCTC GAGGAG(10/8) 199 GAGGAG BsmFI 448 GGGAC GGGAC(10/14) 1018 GTCCC DdeI 85 CTGAG C^T[ACGT]AG 193 CTGAG DraII 245 GGGTCCT [GA]G^G[ACGT]CC[CT] 448 GGGACCC EcoO109I 245 GGGTCCT [GA]G^G[ACGT]CC[CT] 448 GGGACCC EcoT14I 601 CCTTGG C^C[AT][AT]GG 916 CCATGG HphI 606 GGTGA GGTGA(8/7) 477 TCACC PleI 220 GACTC GAGTC(4/5) 87 GAGTC PpuMI 245 GGGTCCT [GA]G^G[AT]CC[CT] 448 GGGACCC StyI 601 CCTTGG C^C[AT][AT]GG 916 CCATGG TaqI 693 TCGA T^CGA 1269 TCGA Tsp45I 565 GTGAC ^GT[GC]AC 956 GTGAC TthHB8I 693 TCGA T^CGA 1269 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI ApoI AvaI AvaII BanI BbvI BcnI BmyI BpmI BsaJI BsgI BsiYI BslI BsmAI BsoBI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I Cfr13I DpnII DsaI EcoRII FauI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII ItaI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SmaI Tru9I TseI Tsp509I TspRI XmaCI XmaI